Biocatalytic and antibacterial visualization of green synthesized silver nanoparticles using Hemidesmus indicus
In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV–visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmi...
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| Veröffentlicht in: | Microbial pathogenesis 2015-05, Vol.82, p.43-49 |
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| creator | Latha, M. Sumathi, M. Manikandan, R. Arumugam, A. Prabhu, N.M. |
| description | In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV–visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 ± 0.2 mm) against isolated bacteria S. sonnei at 40 μg/ml.
[Display omitted]
•Green synthesis of silver nanoparticles is using Hemidesmus indicus.•Silver nanoparticles were investigated by FTIR, XRD, EDX, TEM and UV–vis spectroscopy.•Antibacterial activity is demonstrated using poultry pathogenic bacteria Shigella sonnei.•S. sonnei and silver nanoparticles interaction study in CLSM. |
| doi_str_mv | 10.1016/j.micpath.2015.03.008 |
| format | Article |
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[Display omitted]
•Green synthesis of silver nanoparticles is using Hemidesmus indicus.•Silver nanoparticles were investigated by FTIR, XRD, EDX, TEM and UV–vis spectroscopy.•Antibacterial activity is demonstrated using poultry pathogenic bacteria Shigella sonnei.•S. sonnei and silver nanoparticles interaction study in CLSM.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2015.03.008</identifier><identifier>PMID: 25797527</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - metabolism ; Anti-Bacterial Agents - pharmacology ; Biosynthesis ; CLSM ; Gastrointestinal Tract - microbiology ; Hemidesmus - metabolism ; Hemidesmus indicus ; Microbial Sensitivity Tests ; Microscopy, Electron, Transmission ; Nanoparticles - chemistry ; Nanoparticles - metabolism ; Nanoparticles - microbiology ; Plant Extracts - metabolism ; Plant Leaves - metabolism ; Poultry ; Shigella sonnei ; Shigella sonnei - drug effects ; Shigella sonnei - isolation & purification ; Silver - chemistry ; Silver - metabolism ; Silver - pharmacology ; Silver nanoparticles ; Spectrum Analysis ; X-Ray Diffraction</subject><ispartof>Microbial pathogenesis, 2015-05, Vol.82, p.43-49</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-857f522b13ac64b5f9534050f03521b815b6d3958b1e8dcad491c5d004942b583</citedby><cites>FETCH-LOGICAL-c398t-857f522b13ac64b5f9534050f03521b815b6d3958b1e8dcad491c5d004942b583</cites><orcidid>0000-0003-1141-0526</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2015.03.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25797527$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Latha, M.</creatorcontrib><creatorcontrib>Sumathi, M.</creatorcontrib><creatorcontrib>Manikandan, R.</creatorcontrib><creatorcontrib>Arumugam, A.</creatorcontrib><creatorcontrib>Prabhu, N.M.</creatorcontrib><title>Biocatalytic and antibacterial visualization of green synthesized silver nanoparticles using Hemidesmus indicus</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV–visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 ± 0.2 mm) against isolated bacteria S. sonnei at 40 μg/ml.
[Display omitted]
•Green synthesis of silver nanoparticles is using Hemidesmus indicus.•Silver nanoparticles were investigated by FTIR, XRD, EDX, TEM and UV–vis spectroscopy.•Antibacterial activity is demonstrated using poultry pathogenic bacteria Shigella sonnei.•S. sonnei and silver nanoparticles interaction study in CLSM.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Biosynthesis</subject><subject>CLSM</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Hemidesmus - metabolism</subject><subject>Hemidesmus indicus</subject><subject>Microbial Sensitivity Tests</subject><subject>Microscopy, Electron, Transmission</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - metabolism</subject><subject>Nanoparticles - microbiology</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Leaves - metabolism</subject><subject>Poultry</subject><subject>Shigella sonnei</subject><subject>Shigella sonnei - drug effects</subject><subject>Shigella sonnei - isolation & purification</subject><subject>Silver - chemistry</subject><subject>Silver - metabolism</subject><subject>Silver - pharmacology</subject><subject>Silver nanoparticles</subject><subject>Spectrum Analysis</subject><subject>X-Ray Diffraction</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotvCI4B85JJlbMeJc0JQAUWqxAXOlmNP2lklzmI7K22fvql26bWH0Vy-_x9pPsY-CNgKEM3n3XYiv3flfitB6C2oLYB5xTYCuqYSEsxrtgFjZFWDgAt2mfMOALpadW_ZhdRt12rZbtj8jWbvihuPhTx3MaxTqHe-YCI38gPlxY304ArNkc8Dv0uIkedjLPeY6QEDzzQeMPHo4rx3aa0ZMfMlU7zjNzhRwDwtmVMM5Jf8jr0Z3Jjx_Xlfsb8_vv-5vqluf__8df31tvKqM6Uyuh20lL1Qzjd1r4dOqxo0DKC0FL0Rum-C6rTpBZrgXag74XUAqLta9tqoK_bp1LtP878Fc7ETZY_j6CLOS7aiVdIoqBt4GW1a09ZGGbGi-oT6NOeccLD7RJNLRyvAPmmxO3vWYp-0WFB21bLmPp5PLP2E4Tn138MKfDkBuP7kQJhs9oTRY6CEvtgw0wsnHgHLBqKK</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>Latha, M.</creator><creator>Sumathi, M.</creator><creator>Manikandan, R.</creator><creator>Arumugam, A.</creator><creator>Prabhu, N.M.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-1141-0526</orcidid></search><sort><creationdate>201505</creationdate><title>Biocatalytic and antibacterial visualization of green synthesized silver nanoparticles using Hemidesmus indicus</title><author>Latha, M. ; Sumathi, M. ; Manikandan, R. ; Arumugam, A. ; Prabhu, N.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-857f522b13ac64b5f9534050f03521b815b6d3958b1e8dcad491c5d004942b583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Biosynthesis</topic><topic>CLSM</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>Hemidesmus - metabolism</topic><topic>Hemidesmus indicus</topic><topic>Microbial Sensitivity Tests</topic><topic>Microscopy, Electron, Transmission</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - metabolism</topic><topic>Nanoparticles - microbiology</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Leaves - metabolism</topic><topic>Poultry</topic><topic>Shigella sonnei</topic><topic>Shigella sonnei - drug effects</topic><topic>Shigella sonnei - isolation & purification</topic><topic>Silver - chemistry</topic><topic>Silver - metabolism</topic><topic>Silver - pharmacology</topic><topic>Silver nanoparticles</topic><topic>Spectrum Analysis</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Latha, M.</creatorcontrib><creatorcontrib>Sumathi, M.</creatorcontrib><creatorcontrib>Manikandan, R.</creatorcontrib><creatorcontrib>Arumugam, A.</creatorcontrib><creatorcontrib>Prabhu, N.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Latha, M.</au><au>Sumathi, M.</au><au>Manikandan, R.</au><au>Arumugam, A.</au><au>Prabhu, N.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biocatalytic and antibacterial visualization of green synthesized silver nanoparticles using Hemidesmus indicus</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2015-05</date><risdate>2015</risdate><volume>82</volume><spage>43</spage><epage>49</epage><pages>43-49</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV–visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 ± 0.2 mm) against isolated bacteria S. sonnei at 40 μg/ml.
[Display omitted]
•Green synthesis of silver nanoparticles is using Hemidesmus indicus.•Silver nanoparticles were investigated by FTIR, XRD, EDX, TEM and UV–vis spectroscopy.•Antibacterial activity is demonstrated using poultry pathogenic bacteria Shigella sonnei.•S. sonnei and silver nanoparticles interaction study in CLSM.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25797527</pmid><doi>10.1016/j.micpath.2015.03.008</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1141-0526</orcidid></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Biosynthesis CLSM Gastrointestinal Tract - microbiology Hemidesmus - metabolism Hemidesmus indicus Microbial Sensitivity Tests Microscopy, Electron, Transmission Nanoparticles - chemistry Nanoparticles - metabolism Nanoparticles - microbiology Plant Extracts - metabolism Plant Leaves - metabolism Poultry Shigella sonnei Shigella sonnei - drug effects Shigella sonnei - isolation & purification Silver - chemistry Silver - metabolism Silver - pharmacology Silver nanoparticles Spectrum Analysis X-Ray Diffraction |
| title | Biocatalytic and antibacterial visualization of green synthesized silver nanoparticles using Hemidesmus indicus |
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