Osteoprogenitor Cell Therapy in an Equine Fracture Model
Objective To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model. Study Design Experimental. Animals Adult horses (n = 10). Methods Segmental ostectomies of the 4th metacarpal...
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| Veröffentlicht in: | Veterinary surgery 2012-10, Vol.41 (7), p.773-783 |
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| creator | McDuffee, Laurie A. Pack, LeeAnn Lores, Marcos Wright, Glenda M. Esparza-Gonzalez, Blanca Masaoud, Elmabrok |
| description | Objective
To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model.
Study Design
Experimental.
Animals
Adult horses (n = 10).
Methods
Segmental ostectomies of the 4th metacarpal bone (MC4) were performed bilaterally in 10 horses. There was 1 treatment and 1 control limb in each horse. Bone defects were randomly injected with either fibrin glue and osteoprogenitor cells or fibrin glue alone. Radiography was performed every week until the study endpoint at 12 weeks. After euthanasia, bone healing was evaluated using radiography and histology. Analysis of radiographic data was conducted using a linear‐mixed model. Analysis of histologic data was conducted using a general linear model. Statistical significance was set at P < .05.
Results
Radiographic grayscale data as a measure of bone healing revealed no significant difference between treatment and control limbs. Radiographic scoring results also showed that the treatment effect was not significant. Histologic analysis was consistent with radiographic analysis showing no significant difference between the area of bone present in treatment and control limbs.
Conclusion
Injection of periosteal‐derived osteoprogenitors in a fibrin glue carrier into surgically created ostectomies of MC4 does not accelerate bone healing when compared with fibrin glue alone. |
| doi_str_mv | 10.1111/j.1532-950X.2012.01024.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1732828885</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1113982333</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5894-46683fb07e28e2ec1a156742cb66869f687b7fa72e2e6089f36daf97924a3c513</originalsourceid><addsrcrecordid>eNqNkTtPwzAUhS0EglL4CygSC0uCfe34MTCgqi1vBp6b5aYOpKRJsRvR_nscCh1Y4C62dL5zfX0uQhHBCQl1PElISiFWKX5OABNIMMHAksUG6qyFTdTBhJOYMqV20K73E4yxYoxuox0AiRkw2kHy1s9tPXP1i62Kee2ini3L6P7VOjNbRkUVmSrqvzdFZaOBM9m8cTa6rse23ENbuSm93f8-u-hh0L_vncVXt8Pz3ulVnKVSsZhxLmk-wsKCtGAzYkjKBYNsFASuci7FSORGQBA5liqnfGxyJRQwQ7OU0C46WvUNM7431s_1tPBZGNJUtm68JoKCBCll-jdKCFUSaKguOvyFTurGVeEjLQVMCZA8UHJFZa723tlcz1wxNW6pCW45oie6zVu3eet2EfprEXoRrAffDzSjqR2vjT_JB-BkBXwUpV3-u7F-vHtob8Efr_xFWOBi7TfuTXNBRaqfboZ6eDnAlF3caEw_AaU3oqI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1112497286</pqid></control><display><type>article</type><title>Osteoprogenitor Cell Therapy in an Equine Fracture Model</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>McDuffee, Laurie A. ; Pack, LeeAnn ; Lores, Marcos ; Wright, Glenda M. ; Esparza-Gonzalez, Blanca ; Masaoud, Elmabrok</creator><creatorcontrib>McDuffee, Laurie A. ; Pack, LeeAnn ; Lores, Marcos ; Wright, Glenda M. ; Esparza-Gonzalez, Blanca ; Masaoud, Elmabrok</creatorcontrib><description>Objective
To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model.
Study Design
Experimental.
Animals
Adult horses (n = 10).
Methods
Segmental ostectomies of the 4th metacarpal bone (MC4) were performed bilaterally in 10 horses. There was 1 treatment and 1 control limb in each horse. Bone defects were randomly injected with either fibrin glue and osteoprogenitor cells or fibrin glue alone. Radiography was performed every week until the study endpoint at 12 weeks. After euthanasia, bone healing was evaluated using radiography and histology. Analysis of radiographic data was conducted using a linear‐mixed model. Analysis of histologic data was conducted using a general linear model. Statistical significance was set at P < .05.
Results
Radiographic grayscale data as a measure of bone healing revealed no significant difference between treatment and control limbs. Radiographic scoring results also showed that the treatment effect was not significant. Histologic analysis was consistent with radiographic analysis showing no significant difference between the area of bone present in treatment and control limbs.
Conclusion
Injection of periosteal‐derived osteoprogenitors in a fibrin glue carrier into surgically created ostectomies of MC4 does not accelerate bone healing when compared with fibrin glue alone.</description><identifier>ISSN: 0161-3499</identifier><identifier>EISSN: 1532-950X</identifier><identifier>DOI: 10.1111/j.1532-950X.2012.01024.x</identifier><identifier>PMID: 22804243</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; Disease Models, Animal ; Fibrin Tissue Adhesive - therapeutic use ; Fracture Healing - physiology ; Fractures ; Fractures, Bone - therapy ; Fractures, Bone - veterinary ; Fractures, Ununited - veterinary ; Horses ; Horses - injuries ; Metacarpal Bones - injuries ; Metacarpal Bones - physiology ; Orthopedics ; Osteogenesis - physiology ; Periosteum - cytology ; Random Allocation ; Stem Cell Transplantation - methods ; Stem Cell Transplantation - veterinary ; Stem Cells - cytology ; Stem Cells - physiology ; Surgical outcomes ; Veterinary medicine</subject><ispartof>Veterinary surgery, 2012-10, Vol.41 (7), p.773-783</ispartof><rights>Copyright 2012 by The American College of Veterinary Surgeons</rights><rights>Copyright 2012 by The American College of Veterinary Surgeons.</rights><rights>2012 American College of Veterinary Surgeons</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5894-46683fb07e28e2ec1a156742cb66869f687b7fa72e2e6089f36daf97924a3c513</citedby><cites>FETCH-LOGICAL-c5894-46683fb07e28e2ec1a156742cb66869f687b7fa72e2e6089f36daf97924a3c513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1532-950X.2012.01024.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1532-950X.2012.01024.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22804243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McDuffee, Laurie A.</creatorcontrib><creatorcontrib>Pack, LeeAnn</creatorcontrib><creatorcontrib>Lores, Marcos</creatorcontrib><creatorcontrib>Wright, Glenda M.</creatorcontrib><creatorcontrib>Esparza-Gonzalez, Blanca</creatorcontrib><creatorcontrib>Masaoud, Elmabrok</creatorcontrib><title>Osteoprogenitor Cell Therapy in an Equine Fracture Model</title><title>Veterinary surgery</title><addtitle>Veterinary Surgery</addtitle><description>Objective
To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model.
Study Design
Experimental.
Animals
Adult horses (n = 10).
Methods
Segmental ostectomies of the 4th metacarpal bone (MC4) were performed bilaterally in 10 horses. There was 1 treatment and 1 control limb in each horse. Bone defects were randomly injected with either fibrin glue and osteoprogenitor cells or fibrin glue alone. Radiography was performed every week until the study endpoint at 12 weeks. After euthanasia, bone healing was evaluated using radiography and histology. Analysis of radiographic data was conducted using a linear‐mixed model. Analysis of histologic data was conducted using a general linear model. Statistical significance was set at P < .05.
Results
Radiographic grayscale data as a measure of bone healing revealed no significant difference between treatment and control limbs. Radiographic scoring results also showed that the treatment effect was not significant. Histologic analysis was consistent with radiographic analysis showing no significant difference between the area of bone present in treatment and control limbs.
Conclusion
Injection of periosteal‐derived osteoprogenitors in a fibrin glue carrier into surgically created ostectomies of MC4 does not accelerate bone healing when compared with fibrin glue alone.</description><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>Fibrin Tissue Adhesive - therapeutic use</subject><subject>Fracture Healing - physiology</subject><subject>Fractures</subject><subject>Fractures, Bone - therapy</subject><subject>Fractures, Bone - veterinary</subject><subject>Fractures, Ununited - veterinary</subject><subject>Horses</subject><subject>Horses - injuries</subject><subject>Metacarpal Bones - injuries</subject><subject>Metacarpal Bones - physiology</subject><subject>Orthopedics</subject><subject>Osteogenesis - physiology</subject><subject>Periosteum - cytology</subject><subject>Random Allocation</subject><subject>Stem Cell Transplantation - methods</subject><subject>Stem Cell Transplantation - veterinary</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - physiology</subject><subject>Surgical outcomes</subject><subject>Veterinary medicine</subject><issn>0161-3499</issn><issn>1532-950X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkTtPwzAUhS0EglL4CygSC0uCfe34MTCgqi1vBp6b5aYOpKRJsRvR_nscCh1Y4C62dL5zfX0uQhHBCQl1PElISiFWKX5OABNIMMHAksUG6qyFTdTBhJOYMqV20K73E4yxYoxuox0AiRkw2kHy1s9tPXP1i62Kee2ini3L6P7VOjNbRkUVmSrqvzdFZaOBM9m8cTa6rse23ENbuSm93f8-u-hh0L_vncVXt8Pz3ulVnKVSsZhxLmk-wsKCtGAzYkjKBYNsFASuci7FSORGQBA5liqnfGxyJRQwQ7OU0C46WvUNM7431s_1tPBZGNJUtm68JoKCBCll-jdKCFUSaKguOvyFTurGVeEjLQVMCZA8UHJFZa723tlcz1wxNW6pCW45oie6zVu3eet2EfprEXoRrAffDzSjqR2vjT_JB-BkBXwUpV3-u7F-vHtob8Efr_xFWOBi7TfuTXNBRaqfboZ6eDnAlF3caEw_AaU3oqI</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>McDuffee, Laurie A.</creator><creator>Pack, LeeAnn</creator><creator>Lores, Marcos</creator><creator>Wright, Glenda M.</creator><creator>Esparza-Gonzalez, Blanca</creator><creator>Masaoud, Elmabrok</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>201210</creationdate><title>Osteoprogenitor Cell Therapy in an Equine Fracture Model</title><author>McDuffee, Laurie A. ; Pack, LeeAnn ; Lores, Marcos ; Wright, Glenda M. ; Esparza-Gonzalez, Blanca ; Masaoud, Elmabrok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5894-46683fb07e28e2ec1a156742cb66869f687b7fa72e2e6089f36daf97924a3c513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Disease Models, Animal</topic><topic>Fibrin Tissue Adhesive - therapeutic use</topic><topic>Fracture Healing - physiology</topic><topic>Fractures</topic><topic>Fractures, Bone - therapy</topic><topic>Fractures, Bone - veterinary</topic><topic>Fractures, Ununited - veterinary</topic><topic>Horses</topic><topic>Horses - injuries</topic><topic>Metacarpal Bones - injuries</topic><topic>Metacarpal Bones - physiology</topic><topic>Orthopedics</topic><topic>Osteogenesis - physiology</topic><topic>Periosteum - cytology</topic><topic>Random Allocation</topic><topic>Stem Cell Transplantation - methods</topic><topic>Stem Cell Transplantation - veterinary</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - physiology</topic><topic>Surgical outcomes</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McDuffee, Laurie A.</creatorcontrib><creatorcontrib>Pack, LeeAnn</creatorcontrib><creatorcontrib>Lores, Marcos</creatorcontrib><creatorcontrib>Wright, Glenda M.</creatorcontrib><creatorcontrib>Esparza-Gonzalez, Blanca</creatorcontrib><creatorcontrib>Masaoud, Elmabrok</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Veterinary surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McDuffee, Laurie A.</au><au>Pack, LeeAnn</au><au>Lores, Marcos</au><au>Wright, Glenda M.</au><au>Esparza-Gonzalez, Blanca</au><au>Masaoud, Elmabrok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteoprogenitor Cell Therapy in an Equine Fracture Model</atitle><jtitle>Veterinary surgery</jtitle><addtitle>Veterinary Surgery</addtitle><date>2012-10</date><risdate>2012</risdate><volume>41</volume><issue>7</issue><spage>773</spage><epage>783</epage><pages>773-783</pages><issn>0161-3499</issn><eissn>1532-950X</eissn><abstract>Objective
To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model.
Study Design
Experimental.
Animals
Adult horses (n = 10).
Methods
Segmental ostectomies of the 4th metacarpal bone (MC4) were performed bilaterally in 10 horses. There was 1 treatment and 1 control limb in each horse. Bone defects were randomly injected with either fibrin glue and osteoprogenitor cells or fibrin glue alone. Radiography was performed every week until the study endpoint at 12 weeks. After euthanasia, bone healing was evaluated using radiography and histology. Analysis of radiographic data was conducted using a linear‐mixed model. Analysis of histologic data was conducted using a general linear model. Statistical significance was set at P < .05.
Results
Radiographic grayscale data as a measure of bone healing revealed no significant difference between treatment and control limbs. Radiographic scoring results also showed that the treatment effect was not significant. Histologic analysis was consistent with radiographic analysis showing no significant difference between the area of bone present in treatment and control limbs.
Conclusion
Injection of periosteal‐derived osteoprogenitors in a fibrin glue carrier into surgically created ostectomies of MC4 does not accelerate bone healing when compared with fibrin glue alone.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>22804243</pmid><doi>10.1111/j.1532-950X.2012.01024.x</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0161-3499 |
| ispartof | Veterinary surgery, 2012-10, Vol.41 (7), p.773-783 |
| issn | 0161-3499 1532-950X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1732828885 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Disease Models, Animal Fibrin Tissue Adhesive - therapeutic use Fracture Healing - physiology Fractures Fractures, Bone - therapy Fractures, Bone - veterinary Fractures, Ununited - veterinary Horses Horses - injuries Metacarpal Bones - injuries Metacarpal Bones - physiology Orthopedics Osteogenesis - physiology Periosteum - cytology Random Allocation Stem Cell Transplantation - methods Stem Cell Transplantation - veterinary Stem Cells - cytology Stem Cells - physiology Surgical outcomes Veterinary medicine |
| title | Osteoprogenitor Cell Therapy in an Equine Fracture Model |
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