Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of...
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| Veröffentlicht in: | Fish & shellfish immunology 2015-07, Vol.45 (1), p.157-166 |
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| creator | Rivas-Aravena, Andrea Fuentes, Yazmin Cartagena, Julio Brito, Tania Poggio, Verónica La Torre, José Mendoza, Hegaly Gonzalez-Nilo, Fernando Sandino, Ana María Spencer, Eugenio |
| description | Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.
•Nanoparticles of chitosan with a virine of ISAV and the replicon of SAV administered orally, protected Atlantic salmon against ISAV.•Replicon of SAV strengthened the protection provided by a virine of ISAV in Atlantic salmon.•Replicon of salmon alphavirus strengthened the antiviral immune response triggered by the vaccine. |
| doi_str_mv | 10.1016/j.fsi.2015.03.033 |
| format | Article |
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•Nanoparticles of chitosan with a virine of ISAV and the replicon of SAV administered orally, protected Atlantic salmon against ISAV.•Replicon of SAV strengthened the protection provided by a virine of ISAV in Atlantic salmon.•Replicon of salmon alphavirus strengthened the antiviral immune response triggered by the vaccine.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2015.03.033</identifier><identifier>PMID: 25862072</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adjuvant ; Adjuvants, Immunologic - administration & dosage ; Adjuvants, Immunologic - pharmacology ; Administration, Oral ; Alphavirus ; Alphavirus - genetics ; Alphavirus - immunology ; Animals ; Atlantic salmon ; Fish Diseases - prevention & control ; Fish Diseases - virology ; Infectious salmon anemia virus ; ISAV ; Isavirus - immunology ; Marine ; Nanoparticles ; Nanoparticles - chemistry ; Orthomyxoviridae Infections - prevention & control ; Orthomyxoviridae Infections - veterinary ; Orthomyxoviridae Infections - virology ; Replicon ; Salmo salar ; Salmon alphavirus ; Togaviridae ; Vaccine ; Vaccines, Inactivated - immunology ; Viral Vaccines - pharmacology</subject><ispartof>Fish & shellfish immunology, 2015-07, Vol.45 (1), p.157-166</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-22e79a7d8e9d7ea0a2412d37ff47deacd6c6b9beafac398738d58ad150f073f63</citedby><cites>FETCH-LOGICAL-c499t-22e79a7d8e9d7ea0a2412d37ff47deacd6c6b9beafac398738d58ad150f073f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464815001424$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25862072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rivas-Aravena, Andrea</creatorcontrib><creatorcontrib>Fuentes, Yazmin</creatorcontrib><creatorcontrib>Cartagena, Julio</creatorcontrib><creatorcontrib>Brito, Tania</creatorcontrib><creatorcontrib>Poggio, Verónica</creatorcontrib><creatorcontrib>La Torre, José</creatorcontrib><creatorcontrib>Mendoza, Hegaly</creatorcontrib><creatorcontrib>Gonzalez-Nilo, Fernando</creatorcontrib><creatorcontrib>Sandino, Ana María</creatorcontrib><creatorcontrib>Spencer, Eugenio</creatorcontrib><title>Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.
•Nanoparticles of chitosan with a virine of ISAV and the replicon of SAV administered orally, protected Atlantic salmon against ISAV.•Replicon of SAV strengthened the protection provided by a virine of ISAV in Atlantic salmon.•Replicon of salmon alphavirus strengthened the antiviral immune response triggered by the vaccine.</description><subject>Adjuvant</subject><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Administration, Oral</subject><subject>Alphavirus</subject><subject>Alphavirus - genetics</subject><subject>Alphavirus - immunology</subject><subject>Animals</subject><subject>Atlantic salmon</subject><subject>Fish Diseases - prevention & control</subject><subject>Fish Diseases - virology</subject><subject>Infectious salmon anemia virus</subject><subject>ISAV</subject><subject>Isavirus - immunology</subject><subject>Marine</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Orthomyxoviridae Infections - prevention & control</subject><subject>Orthomyxoviridae Infections - veterinary</subject><subject>Orthomyxoviridae Infections - virology</subject><subject>Replicon</subject><subject>Salmo salar</subject><subject>Salmon alphavirus</subject><subject>Togaviridae</subject><subject>Vaccine</subject><subject>Vaccines, Inactivated - immunology</subject><subject>Viral Vaccines - pharmacology</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0Eou3CD-CCfOSSxR-JnYjTqgVaqRIHPq7WrD0uXiVxsJNI8OtxtIUjSCPNaPS8rzTzEvKKsz1nXL097X0Oe8F4s2eylHxCLjnrmqrrav10mxtW1apuL8hVzifGmJKKPScXommVYFpckl83uGIfpwHHmUZPgY4wxgnSHGyP1REyOhoT9HQFa8OI1MdED3MPYyFohn6II4UHCGOe6d3nwze65DA-UCjbfvoOa0hLpgmnPtiNzBTcaVmL_AV55qHP-PKx78jXD--_XN9W958-3l0f7itbd91cCYG6A-1a7JxGYCBqLpzU3tfaIVinrDp2RwQPVnatlq1rWnC8YZ5p6ZXckTdn3ynFHwvm2QwhW-zLCRiXbLiWohVNrfj_UdUKwVpRXr0j_IzaFHNO6M2UwgDpp-HMbOmYkynpmC0dw2SpTfP60X45Duj-Kv7EUYB3ZwDLP9aAyWQbcLToQkI7GxfDP-x_Ay9koZ4</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Rivas-Aravena, Andrea</creator><creator>Fuentes, Yazmin</creator><creator>Cartagena, Julio</creator><creator>Brito, Tania</creator><creator>Poggio, Verónica</creator><creator>La Torre, José</creator><creator>Mendoza, Hegaly</creator><creator>Gonzalez-Nilo, Fernando</creator><creator>Sandino, Ana María</creator><creator>Spencer, Eugenio</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20150701</creationdate><title>Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant</title><author>Rivas-Aravena, Andrea ; Fuentes, Yazmin ; Cartagena, Julio ; Brito, Tania ; Poggio, Verónica ; La Torre, José ; Mendoza, Hegaly ; Gonzalez-Nilo, Fernando ; Sandino, Ana María ; Spencer, Eugenio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-22e79a7d8e9d7ea0a2412d37ff47deacd6c6b9beafac398738d58ad150f073f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adjuvant</topic><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Administration, Oral</topic><topic>Alphavirus</topic><topic>Alphavirus - genetics</topic><topic>Alphavirus - immunology</topic><topic>Animals</topic><topic>Atlantic salmon</topic><topic>Fish Diseases - prevention & control</topic><topic>Fish Diseases - virology</topic><topic>Infectious salmon anemia virus</topic><topic>ISAV</topic><topic>Isavirus - immunology</topic><topic>Marine</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Orthomyxoviridae Infections - prevention & control</topic><topic>Orthomyxoviridae Infections - veterinary</topic><topic>Orthomyxoviridae Infections - virology</topic><topic>Replicon</topic><topic>Salmo salar</topic><topic>Salmon alphavirus</topic><topic>Togaviridae</topic><topic>Vaccine</topic><topic>Vaccines, Inactivated - immunology</topic><topic>Viral Vaccines - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rivas-Aravena, Andrea</creatorcontrib><creatorcontrib>Fuentes, Yazmin</creatorcontrib><creatorcontrib>Cartagena, Julio</creatorcontrib><creatorcontrib>Brito, Tania</creatorcontrib><creatorcontrib>Poggio, Verónica</creatorcontrib><creatorcontrib>La Torre, José</creatorcontrib><creatorcontrib>Mendoza, Hegaly</creatorcontrib><creatorcontrib>Gonzalez-Nilo, Fernando</creatorcontrib><creatorcontrib>Sandino, Ana María</creatorcontrib><creatorcontrib>Spencer, Eugenio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rivas-Aravena, Andrea</au><au>Fuentes, Yazmin</au><au>Cartagena, Julio</au><au>Brito, Tania</au><au>Poggio, Verónica</au><au>La Torre, José</au><au>Mendoza, Hegaly</au><au>Gonzalez-Nilo, Fernando</au><au>Sandino, Ana María</au><au>Spencer, Eugenio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>45</volume><issue>1</issue><spage>157</spage><epage>166</epage><pages>157-166</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.
•Nanoparticles of chitosan with a virine of ISAV and the replicon of SAV administered orally, protected Atlantic salmon against ISAV.•Replicon of SAV strengthened the protection provided by a virine of ISAV in Atlantic salmon.•Replicon of salmon alphavirus strengthened the antiviral immune response triggered by the vaccine.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25862072</pmid><doi>10.1016/j.fsi.2015.03.033</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adjuvant Adjuvants, Immunologic - administration & dosage Adjuvants, Immunologic - pharmacology Administration, Oral Alphavirus Alphavirus - genetics Alphavirus - immunology Animals Atlantic salmon Fish Diseases - prevention & control Fish Diseases - virology Infectious salmon anemia virus ISAV Isavirus - immunology Marine Nanoparticles Nanoparticles - chemistry Orthomyxoviridae Infections - prevention & control Orthomyxoviridae Infections - veterinary Orthomyxoviridae Infections - virology Replicon Salmo salar Salmon alphavirus Togaviridae Vaccine Vaccines, Inactivated - immunology Viral Vaccines - pharmacology |
| title | Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant |
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