Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces
The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Strep...
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| Veröffentlicht in: | Journal of microbiological methods 2015-06, Vol.113, p.1-3 |
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| container_title | Journal of microbiological methods |
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| creator | Knirschova, Renata Novakova, Renata Mingyar, Erik Bekeova, Carmen Homerova, Dagmar Kormanec, Jan |
| description | The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.
•Reporter system for assessment of gene expression in Streptomyces is described•The system uses the bpsA gene, encoding a biosynthetic enzyme for indigoidine•The reporter was used to construct a plasmid for the deletion of genes•The utility of this system was confirmed by deletion of the actinorhodin genes |
| doi_str_mv | 10.1016/j.mimet.2015.03.017 |
| format | Article |
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•Reporter system for assessment of gene expression in Streptomyces is described•The system uses the bpsA gene, encoding a biosynthetic enzyme for indigoidine•The reporter was used to construct a plasmid for the deletion of genes•The utility of this system was confirmed by deletion of the actinorhodin genes</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2015.03.017</identifier><identifier>PMID: 25801098</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Actinorhodin ; Gene disruption ; Genes, Bacterial ; Genes, Reporter ; Indigoidine ; Peptide Synthases - genetics ; Piperidones - metabolism ; Plasmids ; Promoter Regions, Genetic ; Promoter-probe vector ; Recombination, Genetic ; Reporter gene ; Sequence Deletion ; Streptomyces ; Streptomyces aureofaciens ; Streptomyces aureofaciens - enzymology ; Streptomyces aureofaciens - genetics</subject><ispartof>Journal of microbiological methods, 2015-06, Vol.113, p.1-3</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-a038e17053adec5c29aadd075df89b9293ff7228e32a61b6d3d15f5f740c8f153</citedby><cites>FETCH-LOGICAL-c392t-a038e17053adec5c29aadd075df89b9293ff7228e32a61b6d3d15f5f740c8f153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2015.03.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25801098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Knirschova, Renata</creatorcontrib><creatorcontrib>Novakova, Renata</creatorcontrib><creatorcontrib>Mingyar, Erik</creatorcontrib><creatorcontrib>Bekeova, Carmen</creatorcontrib><creatorcontrib>Homerova, Dagmar</creatorcontrib><creatorcontrib>Kormanec, Jan</creatorcontrib><title>Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.
•Reporter system for assessment of gene expression in Streptomyces is described•The system uses the bpsA gene, encoding a biosynthetic enzyme for indigoidine•The reporter was used to construct a plasmid for the deletion of genes•The utility of this system was confirmed by deletion of the actinorhodin genes</description><subject>Actinorhodin</subject><subject>Gene disruption</subject><subject>Genes, Bacterial</subject><subject>Genes, Reporter</subject><subject>Indigoidine</subject><subject>Peptide Synthases - genetics</subject><subject>Piperidones - metabolism</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Promoter-probe vector</subject><subject>Recombination, Genetic</subject><subject>Reporter gene</subject><subject>Sequence Deletion</subject><subject>Streptomyces</subject><subject>Streptomyces aureofaciens</subject><subject>Streptomyces aureofaciens - enzymology</subject><subject>Streptomyces aureofaciens - genetics</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EokvhCZCQj1wS_Ge9dg4cqqpQpEocoGfLsceLV0kcbG-l9IF4Thy2yxFOI8385pvR9yH0lpKWErr7cGjHMEJpGaGiJbwlVD5DG6okaxQX3XO0qZRsJKHsAr3K-UAqyLfqJbpgQhFKOrVBv-5LGMKjKSFOOHpscII5pgIJ5yUXGHFvMjhcp-UH4H44Ap7DfoSp4DC5sI_BhakOQszLVJESLN7D2pnzFfYxYQcF7Fl_TnGMq7qprYdQFmwmV5EBzsS6nKs2_lbqKyWOi4X8Gr3wZsjw5qleovtPN9-vb5u7r5-_XF_dNZZ3rDSGcAVUEsGNAyss64xxjkjhvOr6jnXce8mYAs7MjvY7xx0VXni5JVb56s4len_SrX_-PEIuegzZwjCYCeIxayo5U4wqrv6P7hTphOjktqL8hNoUc07g9ZzCaNKiKdFrlvqg_2Sp1yw14bpmWbfePR049iO4vzvn8Crw8QRAdeQhQNLZBpgsuJCq49rF8M8DvwE6BLTa</recordid><startdate>201506</startdate><enddate>201506</enddate><creator>Knirschova, Renata</creator><creator>Novakova, Renata</creator><creator>Mingyar, Erik</creator><creator>Bekeova, Carmen</creator><creator>Homerova, Dagmar</creator><creator>Kormanec, Jan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201506</creationdate><title>Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces</title><author>Knirschova, Renata ; Novakova, Renata ; Mingyar, Erik ; Bekeova, Carmen ; Homerova, Dagmar ; Kormanec, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-a038e17053adec5c29aadd075df89b9293ff7228e32a61b6d3d15f5f740c8f153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Actinorhodin</topic><topic>Gene disruption</topic><topic>Genes, Bacterial</topic><topic>Genes, Reporter</topic><topic>Indigoidine</topic><topic>Peptide Synthases - genetics</topic><topic>Piperidones - metabolism</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Promoter-probe vector</topic><topic>Recombination, Genetic</topic><topic>Reporter gene</topic><topic>Sequence Deletion</topic><topic>Streptomyces</topic><topic>Streptomyces aureofaciens</topic><topic>Streptomyces aureofaciens - enzymology</topic><topic>Streptomyces aureofaciens - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knirschova, Renata</creatorcontrib><creatorcontrib>Novakova, Renata</creatorcontrib><creatorcontrib>Mingyar, Erik</creatorcontrib><creatorcontrib>Bekeova, Carmen</creatorcontrib><creatorcontrib>Homerova, Dagmar</creatorcontrib><creatorcontrib>Kormanec, Jan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knirschova, Renata</au><au>Novakova, Renata</au><au>Mingyar, Erik</au><au>Bekeova, Carmen</au><au>Homerova, Dagmar</au><au>Kormanec, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2015-06</date><risdate>2015</risdate><volume>113</volume><spage>1</spage><epage>3</epage><pages>1-3</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>The integrative promoter-probe plasmid pBPSA1 was constructed using a promoterless Streptomyces aureofaciens CCM3239 bpsA gene encoding a non-ribosomal peptide synthase for the biosynthesis of a blue pigment, indigoidine. bpsA was also used to prepare pAMR4 plasmid for the deletion of genes in Streptomyces with facile identification of double crossover recombination.
•Reporter system for assessment of gene expression in Streptomyces is described•The system uses the bpsA gene, encoding a biosynthetic enzyme for indigoidine•The reporter was used to construct a plasmid for the deletion of genes•The utility of this system was confirmed by deletion of the actinorhodin genes</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25801098</pmid><doi>10.1016/j.mimet.2015.03.017</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2015-06, Vol.113, p.1-3 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Actinorhodin Gene disruption Genes, Bacterial Genes, Reporter Indigoidine Peptide Synthases - genetics Piperidones - metabolism Plasmids Promoter Regions, Genetic Promoter-probe vector Recombination, Genetic Reporter gene Sequence Deletion Streptomyces Streptomyces aureofaciens Streptomyces aureofaciens - enzymology Streptomyces aureofaciens - genetics |
| title | Utilization of a reporter system based on the blue pigment indigoidine biosynthetic gene bpsA for detection of promoter activity and deletion of genes in Streptomyces |
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