Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease
•We generated a recombinant VLP vaccine against FMDV.•VLP can produce high neutralizing antibody against FMDV.•The enhanced protection effect was demonstrated in pigs, mice and guinea pigs. Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work,...
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| Veröffentlicht in: | Antiviral research 2015-05, Vol.117, p.39-43 |
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| container_title | Antiviral research |
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| creator | Dong, Yan-mei Zhang, Guo-guang Huang, Xiao-jun Chen, Liang Chen, Hao-tai |
| description | •We generated a recombinant VLP vaccine against FMDV.•VLP can produce high neutralizing antibody against FMDV.•The enhanced protection effect was demonstrated in pigs, mice and guinea pigs.
Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work, the coding genes of 141–160 epitope peptide (EP141–160) of VP1 were inserted into the coat protein (CP) genes of MS2 in prokaryotic expression vector, and the recombinant protein self-assembled into virus-like particles (VLP). Results showed that the CP–EP141–160 VLP had a strong immunoreaction with the FMD virus (FMDV) antigen in vitro, and also had an effective immune response in mice. Further virus challenge tests were carried out on guinea pigs and swine, high-titer neutralizing antibodies were produced and the CP–EP141–160 VLP vaccine could protect most of the animals against FMDV. |
| doi_str_mv | 10.1016/j.antiviral.2015.01.005 |
| format | Article |
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Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work, the coding genes of 141–160 epitope peptide (EP141–160) of VP1 were inserted into the coat protein (CP) genes of MS2 in prokaryotic expression vector, and the recombinant protein self-assembled into virus-like particles (VLP). Results showed that the CP–EP141–160 VLP had a strong immunoreaction with the FMD virus (FMDV) antigen in vitro, and also had an effective immune response in mice. Further virus challenge tests were carried out on guinea pigs and swine, high-titer neutralizing antibodies were produced and the CP–EP141–160 VLP vaccine could protect most of the animals against FMDV.</description><identifier>ISSN: 0166-3542</identifier><identifier>EISSN: 1872-9096</identifier><identifier>DOI: 10.1016/j.antiviral.2015.01.005</identifier><identifier>PMID: 25676866</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Neutralizing - biosynthesis ; Antibodies, Viral - biosynthesis ; Antibodies, Viral - immunology ; Capsid Proteins - genetics ; Capsid Proteins - immunology ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; Foot-and-mouth disease (FMD) ; Foot-and-Mouth Disease - immunology ; Foot-and-Mouth Disease - prevention & control ; Foot-and-mouth disease virus ; Foot-and-Mouth Disease Virus - genetics ; Foot-and-Mouth Disease Virus - immunology ; Freund's Adjuvant ; Guinea Pigs ; Immunity ; Levivirus - genetics ; Mice ; Neutralization Tests ; Swine ; Vaccine ; Vaccines, Virus-Like Particle - administration & dosage ; Vaccines, Virus-Like Particle - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - immunology ; Virus-like particles (VLP)</subject><ispartof>Antiviral research, 2015-05, Vol.117, p.39-43</ispartof><rights>2015</rights><rights>Copyright © 2015. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-62929960b8c3bc27ff7540cbbae86a6ae8a64562b9c6e6319090951944e3e5683</citedby><cites>FETCH-LOGICAL-c404t-62929960b8c3bc27ff7540cbbae86a6ae8a64562b9c6e6319090951944e3e5683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.antiviral.2015.01.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25676866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Yan-mei</creatorcontrib><creatorcontrib>Zhang, Guo-guang</creatorcontrib><creatorcontrib>Huang, Xiao-jun</creatorcontrib><creatorcontrib>Chen, Liang</creatorcontrib><creatorcontrib>Chen, Hao-tai</creatorcontrib><title>Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease</title><title>Antiviral research</title><addtitle>Antiviral Res</addtitle><description>•We generated a recombinant VLP vaccine against FMDV.•VLP can produce high neutralizing antibody against FMDV.•The enhanced protection effect was demonstrated in pigs, mice and guinea pigs.
Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work, the coding genes of 141–160 epitope peptide (EP141–160) of VP1 were inserted into the coat protein (CP) genes of MS2 in prokaryotic expression vector, and the recombinant protein self-assembled into virus-like particles (VLP). Results showed that the CP–EP141–160 VLP had a strong immunoreaction with the FMD virus (FMDV) antigen in vitro, and also had an effective immune response in mice. Further virus challenge tests were carried out on guinea pigs and swine, high-titer neutralizing antibodies were produced and the CP–EP141–160 VLP vaccine could protect most of the animals against FMDV.</description><subject>Animals</subject><subject>Antibodies, Neutralizing - biosynthesis</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibodies, Viral - immunology</subject><subject>Capsid Proteins - genetics</subject><subject>Capsid Proteins - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes</subject><subject>Foot-and-mouth disease (FMD)</subject><subject>Foot-and-Mouth Disease - immunology</subject><subject>Foot-and-Mouth Disease - prevention & control</subject><subject>Foot-and-mouth disease virus</subject><subject>Foot-and-Mouth Disease Virus - genetics</subject><subject>Foot-and-Mouth Disease Virus - immunology</subject><subject>Freund's Adjuvant</subject><subject>Guinea Pigs</subject><subject>Immunity</subject><subject>Levivirus - genetics</subject><subject>Mice</subject><subject>Neutralization Tests</subject><subject>Swine</subject><subject>Vaccine</subject><subject>Vaccines, Virus-Like Particle - administration & dosage</subject><subject>Vaccines, Virus-Like Particle - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - immunology</subject><subject>Virus-like particles (VLP)</subject><issn>0166-3542</issn><issn>1872-9096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhi1UBMvHX2hz7CXp2Ekm9hGtCq20FSDas-U4E-ptNtnazkr8e4wW9rqXmcsz8-p9GPvCoeDA8du6MGN0O-fNUAjgdQG8AKhP2ILLRuQKFH5ii0RiXtaVOGcXIawBABslz9i5qLFBibhgjw9-2rjgxufs15PINtQ5E6nL0us55IP7R9nW-OjsQNnOWOtGysyzcWOIWT9NMTdjl2-mOf7NOhfIBLpip70ZAl2_70v25_b77-WPfHV_93N5s8ptBVXMUSihFEIrbdla0fR9U1dg29aQRINpGqxqFK2ySFjyVAlUzVVVUUk1yvKSfd3_3frp_0wh6tTD0jCYkaY5aN6UQookAo6j2HDBpeRlQps9av0Ugqdeb73bGP-iOeg39XqtD-r1m3oNXCf16fLze8jcJo2Huw_XCbjZA5Ss7Bx5Hayj0SblnmzU3eSOhrwCdjqYfw</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Dong, Yan-mei</creator><creator>Zhang, Guo-guang</creator><creator>Huang, Xiao-jun</creator><creator>Chen, Liang</creator><creator>Chen, Hao-tai</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20150501</creationdate><title>Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease</title><author>Dong, Yan-mei ; Zhang, Guo-guang ; Huang, Xiao-jun ; Chen, Liang ; Chen, Hao-tai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-62929960b8c3bc27ff7540cbbae86a6ae8a64562b9c6e6319090951944e3e5683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antibodies, Neutralizing - biosynthesis</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibodies, Viral - immunology</topic><topic>Capsid Proteins - genetics</topic><topic>Capsid Proteins - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes</topic><topic>Foot-and-mouth disease (FMD)</topic><topic>Foot-and-Mouth Disease - immunology</topic><topic>Foot-and-Mouth Disease - prevention & control</topic><topic>Foot-and-mouth disease virus</topic><topic>Foot-and-Mouth Disease Virus - genetics</topic><topic>Foot-and-Mouth Disease Virus - immunology</topic><topic>Freund's Adjuvant</topic><topic>Guinea Pigs</topic><topic>Immunity</topic><topic>Levivirus - genetics</topic><topic>Mice</topic><topic>Neutralization Tests</topic><topic>Swine</topic><topic>Vaccine</topic><topic>Vaccines, Virus-Like Particle - administration & dosage</topic><topic>Vaccines, Virus-Like Particle - immunology</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - immunology</topic><topic>Virus-like particles (VLP)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Yan-mei</creatorcontrib><creatorcontrib>Zhang, Guo-guang</creatorcontrib><creatorcontrib>Huang, Xiao-jun</creatorcontrib><creatorcontrib>Chen, Liang</creatorcontrib><creatorcontrib>Chen, Hao-tai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Antiviral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Yan-mei</au><au>Zhang, Guo-guang</au><au>Huang, Xiao-jun</au><au>Chen, Liang</au><au>Chen, Hao-tai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease</atitle><jtitle>Antiviral research</jtitle><addtitle>Antiviral Res</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>117</volume><spage>39</spage><epage>43</epage><pages>39-43</pages><issn>0166-3542</issn><eissn>1872-9096</eissn><abstract>•We generated a recombinant VLP vaccine against FMDV.•VLP can produce high neutralizing antibody against FMDV.•The enhanced protection effect was demonstrated in pigs, mice and guinea pigs.
Foot-and-mouth disease (FMD) has caused severe economic losses to millions of farmers worldwide. In this work, the coding genes of 141–160 epitope peptide (EP141–160) of VP1 were inserted into the coat protein (CP) genes of MS2 in prokaryotic expression vector, and the recombinant protein self-assembled into virus-like particles (VLP). Results showed that the CP–EP141–160 VLP had a strong immunoreaction with the FMD virus (FMDV) antigen in vitro, and also had an effective immune response in mice. Further virus challenge tests were carried out on guinea pigs and swine, high-titer neutralizing antibodies were produced and the CP–EP141–160 VLP vaccine could protect most of the animals against FMDV.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25676866</pmid><doi>10.1016/j.antiviral.2015.01.005</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Antibodies, Neutralizing - biosynthesis Antibodies, Viral - biosynthesis Antibodies, Viral - immunology Capsid Proteins - genetics Capsid Proteins - immunology Enzyme-Linked Immunosorbent Assay Epitopes Foot-and-mouth disease (FMD) Foot-and-Mouth Disease - immunology Foot-and-Mouth Disease - prevention & control Foot-and-mouth disease virus Foot-and-Mouth Disease Virus - genetics Foot-and-Mouth Disease Virus - immunology Freund's Adjuvant Guinea Pigs Immunity Levivirus - genetics Mice Neutralization Tests Swine Vaccine Vaccines, Virus-Like Particle - administration & dosage Vaccines, Virus-Like Particle - immunology Viral Vaccines - administration & dosage Viral Vaccines - immunology Virus-like particles (VLP) |
| title | Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease |
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