Bone Marrow-Engrafted Cells after Mice Umbilical Cord Blood Transplantation Differentiate into Osteoblastic Cells in Response to Fracture and Placement of Titanium Screws
As the in vivo function of bone marrow-engrafted umbilical cord blood (UCB)-derived mesenchymal cells (UCBCs) after UCB transplantation is unknown, we examined in vivo osteoblastic differentiation using mouse UCB transplantation and fracture models. UCBCs obtained from GFP transgenic mice were intra...
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| Veröffentlicht in: | Experimental Animals 2012, Vol.61(4), pp.427-433 |
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| creator | Uchida, Kentaro Ueno, Masaki Naruse, Kouji Urabe, Ken Onuma, Kenji Sakai, Rina Itoman, Moritoshi Takaso, Masashi |
| description | As the in vivo function of bone marrow-engrafted umbilical cord blood (UCB)-derived mesenchymal cells (UCBCs) after UCB transplantation is unknown, we examined in vivo osteoblastic differentiation using mouse UCB transplantation and fracture models. UCBCs obtained from GFP transgenic mice were intravenously injected into irradiated C57BL/6 mice. After three months, the in vivo osteoblastic differentiation potential of bone marrow-engrafted UCBCs was examined histologically using a mouse fracture model. GFP-positive UCBCs were detected in the bone marrow of recipient mice. On day 7, UCBCs were observed in the fracture gap and surrounding the titanium screws of the fixation device. The UCBCs were also positive for alkaline phosphatase and von Kossa staining. By day 14, UCBCs were observed around and within a formed intramedullary callus. The newly formed woven bone consisted of ALP- and von Kossa-positive cells. Our findings suggest that UCBCs contribute to the fracture healing process after bone marrow engraftment and that UCBC transplantation can fully reconstruct not only hematopoietic cells but also mesenchymal cell lineages. |
| doi_str_mv | 10.1538/expanim.61.427 |
| format | Article |
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UCBCs obtained from GFP transgenic mice were intravenously injected into irradiated C57BL/6 mice. After three months, the in vivo osteoblastic differentiation potential of bone marrow-engrafted UCBCs was examined histologically using a mouse fracture model. GFP-positive UCBCs were detected in the bone marrow of recipient mice. On day 7, UCBCs were observed in the fracture gap and surrounding the titanium screws of the fixation device. The UCBCs were also positive for alkaline phosphatase and von Kossa staining. By day 14, UCBCs were observed around and within a formed intramedullary callus. The newly formed woven bone consisted of ALP- and von Kossa-positive cells. Our findings suggest that UCBCs contribute to the fracture healing process after bone marrow engraftment and that UCBC transplantation can fully reconstruct not only hematopoietic cells but also mesenchymal cell lineages.</description><identifier>ISSN: 1341-1357</identifier><identifier>EISSN: 1881-7122</identifier><identifier>DOI: 10.1538/expanim.61.427</identifier><identifier>PMID: 22850642</identifier><language>eng</language><publisher>Japan: Japanese Association for Laboratory Animal Science</publisher><subject>Animals ; Bone Marrow Cells ; Bone Plates ; Bone Screws ; Bony Callus - cytology ; Bony Callus - physiology ; Cell Differentiation ; Cell Lineage ; Cord Blood Stem Cell Transplantation ; Disease Models, Animal ; Female ; Femoral Fractures - physiopathology ; Fetal Stem Cells - chemistry ; Fetal Stem Cells - cytology ; Flow Cytometry ; Fracture Healing ; Green Fluorescent Proteins - chemistry ; Humans ; in vivo ; Injections, Intravenous ; mesenchymal cells ; Mice ; Mice, Inbred C57BL ; osteoblastic differentiation potential ; Osteoblasts - cytology ; Osteogenesis ; Titanium ; transplantation ; Transplantation Chimera ; umbilical cord blood</subject><ispartof>Experimental Animals, 2012, Vol.61(4), pp.427-433</ispartof><rights>2012 Japanese Association for Laboratory Animal Science</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-9808cee81a75a1d391768cc338d0bcc08c5ddbd14ae22b06b29a0dd009af5c683</citedby><cites>FETCH-LOGICAL-c483t-9808cee81a75a1d391768cc338d0bcc08c5ddbd14ae22b06b29a0dd009af5c683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22850642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uchida, Kentaro</creatorcontrib><creatorcontrib>Ueno, Masaki</creatorcontrib><creatorcontrib>Naruse, Kouji</creatorcontrib><creatorcontrib>Urabe, Ken</creatorcontrib><creatorcontrib>Onuma, Kenji</creatorcontrib><creatorcontrib>Sakai, Rina</creatorcontrib><creatorcontrib>Itoman, Moritoshi</creatorcontrib><creatorcontrib>Takaso, Masashi</creatorcontrib><title>Bone Marrow-Engrafted Cells after Mice Umbilical Cord Blood Transplantation Differentiate into Osteoblastic Cells in Response to Fracture and Placement of Titanium Screws</title><title>Experimental Animals</title><addtitle>Exp Anim</addtitle><description>As the in vivo function of bone marrow-engrafted umbilical cord blood (UCB)-derived mesenchymal cells (UCBCs) after UCB transplantation is unknown, we examined in vivo osteoblastic differentiation using mouse UCB transplantation and fracture models. UCBCs obtained from GFP transgenic mice were intravenously injected into irradiated C57BL/6 mice. After three months, the in vivo osteoblastic differentiation potential of bone marrow-engrafted UCBCs was examined histologically using a mouse fracture model. GFP-positive UCBCs were detected in the bone marrow of recipient mice. On day 7, UCBCs were observed in the fracture gap and surrounding the titanium screws of the fixation device. The UCBCs were also positive for alkaline phosphatase and von Kossa staining. By day 14, UCBCs were observed around and within a formed intramedullary callus. The newly formed woven bone consisted of ALP- and von Kossa-positive cells. Our findings suggest that UCBCs contribute to the fracture healing process after bone marrow engraftment and that UCBC transplantation can fully reconstruct not only hematopoietic cells but also mesenchymal cell lineages.</description><subject>Animals</subject><subject>Bone Marrow Cells</subject><subject>Bone Plates</subject><subject>Bone Screws</subject><subject>Bony Callus - cytology</subject><subject>Bony Callus - physiology</subject><subject>Cell Differentiation</subject><subject>Cell Lineage</subject><subject>Cord Blood Stem Cell Transplantation</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Femoral Fractures - physiopathology</subject><subject>Fetal Stem Cells - chemistry</subject><subject>Fetal Stem Cells - cytology</subject><subject>Flow Cytometry</subject><subject>Fracture Healing</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Humans</subject><subject>in vivo</subject><subject>Injections, Intravenous</subject><subject>mesenchymal cells</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>osteoblastic differentiation potential</subject><subject>Osteoblasts - cytology</subject><subject>Osteogenesis</subject><subject>Titanium</subject><subject>transplantation</subject><subject>Transplantation Chimera</subject><subject>umbilical cord blood</subject><issn>1341-1357</issn><issn>1881-7122</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtv1TAQRiMEog_YskRessnFjzycZXv7AKlVEdyuo4k9Ka4cO9iOCn-JX4nLDVdiNSPN8dGMv6J4x-iG1UJ-xJ8zODNtGrapePuiOGZSsrJlnL_MvahYyUTdHhUnMT5SytuWd6-LI85lTZuKHxe_z71Dcgsh-Kfy0j0EGBNqskVrI3nuA7k1Csn9NBhrFFiy9UGTc-u9JrsALs4WXIJkvCMXZhwxoEsGEhLjkid3MaEfLMRk1Go1jnzFOHsXkWTiKoBKS0ACTpMvFhRO2UD8SHYm5duWiXxTAZ_im-LVCDbi27WeFvdXl7vtp_Lm7vrz9uymVJUUqewklQpRMmhrYFp0rG2kUkJITQel8rDWetCsAuR8oM3AO6BaU9rBWKtGitPiw947B_9jwZj6yUSVVweHfok9awWXnAreZnSzR1XwMQYc-zmYCcKvntH-OZ9-zadvWF_9ffB-dS_DhPqA_wskA2d74DEmeMADACF_oMX_fKv0MFPfIfToxB8QPqkP</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Uchida, Kentaro</creator><creator>Ueno, Masaki</creator><creator>Naruse, Kouji</creator><creator>Urabe, Ken</creator><creator>Onuma, Kenji</creator><creator>Sakai, Rina</creator><creator>Itoman, Moritoshi</creator><creator>Takaso, Masashi</creator><general>Japanese Association for Laboratory Animal Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope></search><sort><creationdate>2012</creationdate><title>Bone Marrow-Engrafted Cells after Mice Umbilical Cord Blood Transplantation Differentiate into Osteoblastic Cells in Response to Fracture and Placement of Titanium Screws</title><author>Uchida, Kentaro ; Ueno, Masaki ; Naruse, Kouji ; Urabe, Ken ; Onuma, Kenji ; Sakai, Rina ; Itoman, Moritoshi ; Takaso, Masashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-9808cee81a75a1d391768cc338d0bcc08c5ddbd14ae22b06b29a0dd009af5c683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Bone Marrow Cells</topic><topic>Bone Plates</topic><topic>Bone Screws</topic><topic>Bony Callus - cytology</topic><topic>Bony Callus - physiology</topic><topic>Cell Differentiation</topic><topic>Cell Lineage</topic><topic>Cord Blood Stem Cell Transplantation</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Femoral Fractures - physiopathology</topic><topic>Fetal Stem Cells - chemistry</topic><topic>Fetal Stem Cells - cytology</topic><topic>Flow Cytometry</topic><topic>Fracture Healing</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Humans</topic><topic>in vivo</topic><topic>Injections, Intravenous</topic><topic>mesenchymal cells</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>osteoblastic differentiation potential</topic><topic>Osteoblasts - cytology</topic><topic>Osteogenesis</topic><topic>Titanium</topic><topic>transplantation</topic><topic>Transplantation Chimera</topic><topic>umbilical cord blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uchida, Kentaro</creatorcontrib><creatorcontrib>Ueno, Masaki</creatorcontrib><creatorcontrib>Naruse, Kouji</creatorcontrib><creatorcontrib>Urabe, Ken</creatorcontrib><creatorcontrib>Onuma, Kenji</creatorcontrib><creatorcontrib>Sakai, Rina</creatorcontrib><creatorcontrib>Itoman, Moritoshi</creatorcontrib><creatorcontrib>Takaso, Masashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Experimental Animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uchida, Kentaro</au><au>Ueno, Masaki</au><au>Naruse, Kouji</au><au>Urabe, Ken</au><au>Onuma, Kenji</au><au>Sakai, Rina</au><au>Itoman, Moritoshi</au><au>Takaso, Masashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone Marrow-Engrafted Cells after Mice Umbilical Cord Blood Transplantation Differentiate into Osteoblastic Cells in Response to Fracture and Placement of Titanium Screws</atitle><jtitle>Experimental Animals</jtitle><addtitle>Exp Anim</addtitle><date>2012</date><risdate>2012</risdate><volume>61</volume><issue>4</issue><spage>427</spage><epage>433</epage><pages>427-433</pages><issn>1341-1357</issn><eissn>1881-7122</eissn><abstract>As the in vivo function of bone marrow-engrafted umbilical cord blood (UCB)-derived mesenchymal cells (UCBCs) after UCB transplantation is unknown, we examined in vivo osteoblastic differentiation using mouse UCB transplantation and fracture models. UCBCs obtained from GFP transgenic mice were intravenously injected into irradiated C57BL/6 mice. After three months, the in vivo osteoblastic differentiation potential of bone marrow-engrafted UCBCs was examined histologically using a mouse fracture model. GFP-positive UCBCs were detected in the bone marrow of recipient mice. On day 7, UCBCs were observed in the fracture gap and surrounding the titanium screws of the fixation device. The UCBCs were also positive for alkaline phosphatase and von Kossa staining. By day 14, UCBCs were observed around and within a formed intramedullary callus. The newly formed woven bone consisted of ALP- and von Kossa-positive cells. Our findings suggest that UCBCs contribute to the fracture healing process after bone marrow engraftment and that UCBC transplantation can fully reconstruct not only hematopoietic cells but also mesenchymal cell lineages.</abstract><cop>Japan</cop><pub>Japanese Association for Laboratory Animal Science</pub><pmid>22850642</pmid><doi>10.1538/expanim.61.427</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bone Marrow Cells Bone Plates Bone Screws Bony Callus - cytology Bony Callus - physiology Cell Differentiation Cell Lineage Cord Blood Stem Cell Transplantation Disease Models, Animal Female Femoral Fractures - physiopathology Fetal Stem Cells - chemistry Fetal Stem Cells - cytology Flow Cytometry Fracture Healing Green Fluorescent Proteins - chemistry Humans in vivo Injections, Intravenous mesenchymal cells Mice Mice, Inbred C57BL osteoblastic differentiation potential Osteoblasts - cytology Osteogenesis Titanium transplantation Transplantation Chimera umbilical cord blood |
| title | Bone Marrow-Engrafted Cells after Mice Umbilical Cord Blood Transplantation Differentiate into Osteoblastic Cells in Response to Fracture and Placement of Titanium Screws |
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