Efficient genetic engineering of human intestinal organoids using electroporation

This protocol describes how to grow untransformed human colonic organoids and deliver genes of interest into the organoids via the piggyBac transposon or gene editing using the CRISPR-Cas9 system. Gene modification in untransformed human intestinal cells is an attractive approach for studying gene f...

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Veröffentlicht in:	Nature protocols 2015-10, Vol.10 (10), p.1474-1485
Hauptverfasser:	Fujii, Masayuki, Matano, Mami, Nanki, Kosaku, Sato, Toshiro
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1407/651 631/1647/2300/1514 631/1647/767/2199 631/532/2437 Analytical Chemistry Animals Biological Techniques Cell Culture Techniques Cell Line Computational Biology/Bioinformatics Electroporation Flow Cytometry Genetic engineering Genetic Engineering - methods Genetic vectors Growth factors Humans Intestines - cytology Life Sciences Methods Mice Microarrays Organic Chemistry Organoids - cytology protocol
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