A Monoclonal Antibody Reactive with a 40-kDa Molecule on Fetal Thymocytes and Tumor Cells Blocks Proliferation and Stimulates Aggregation and Apoptosis

E710.2.3 is a murine thymic lymphoma cell line with an immature phenotype (CD4-CD8-) that proliferates in response to thymocytes or PMA when cultured at low density and proliferates spontaneously when grown at high density. To identify functional molecules on this cell line, we screened for mAbs tha...

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Veröffentlicht in:The Journal of immunology (1950) 1999-08, Vol.163 (3), p.1306-1314
Hauptverfasser: Fernandes, Dancella M, Baird, Allison M, Berg, Leslie J, Rock, Kenneth L
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Baird, Allison M
Berg, Leslie J
Rock, Kenneth L
description E710.2.3 is a murine thymic lymphoma cell line with an immature phenotype (CD4-CD8-) that proliferates in response to thymocytes or PMA when cultured at low density and proliferates spontaneously when grown at high density. To identify functional molecules on this cell line, we screened for mAbs that could block its proliferation. A hamster mAb, DMF10.62.3, inhibited the spontaneous, thymocyte-induced, and PMA-stimulated proliferation of E710.2.3 in vitro and induced these cells to undergo apoptosis. The mAb also caused homotypic aggregation of E710.2.3, which was inhibited by cytochalasin B, trifluoperazine, a combination of sodium azide and 2-deoxyglucose, EDTA, incubation at 4 degrees C, or treatment with paraformaldehyde. The DMF10 62.3 mAb stained a number of immortalized murine and human cell lines and, where tested, blocked their proliferation and caused death to varying extents by apoptosis. The molecule recognized by the mAb DMF10.62.3 was expressed on day 14 fetal thymus Thy1.2-positive cells. However, it was not detected on adult murine thymocytes, splenocytes, or bone marrow cells or on splenic LPS-activated B cells or Con A-activated T cells. The Ab immunoprecipitated a 40-kDa molecule from E710.2.3 that was not glycosylphosphatidylinositol linked. The data suggest that the molecule recognized by DMF62.3 is a novel cell surface molecule that may be involved in cell proliferation and/or cell death.
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To identify functional molecules on this cell line, we screened for mAbs that could block its proliferation. A hamster mAb, DMF10.62.3, inhibited the spontaneous, thymocyte-induced, and PMA-stimulated proliferation of E710.2.3 in vitro and induced these cells to undergo apoptosis. The mAb also caused homotypic aggregation of E710.2.3, which was inhibited by cytochalasin B, trifluoperazine, a combination of sodium azide and 2-deoxyglucose, EDTA, incubation at 4 degrees C, or treatment with paraformaldehyde. The DMF10 62.3 mAb stained a number of immortalized murine and human cell lines and, where tested, blocked their proliferation and caused death to varying extents by apoptosis. The molecule recognized by the mAb DMF10.62.3 was expressed on day 14 fetal thymus Thy1.2-positive cells. However, it was not detected on adult murine thymocytes, splenocytes, or bone marrow cells or on splenic LPS-activated B cells or Con A-activated T cells. 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The Ab immunoprecipitated a 40-kDa molecule from E710.2.3 that was not glycosylphosphatidylinositol linked. The data suggest that the molecule recognized by DMF62.3 is a novel cell surface molecule that may be involved in cell proliferation and/or cell death.</description><subject>Animals</subject><subject>Antibodies, Blocking - metabolism</subject><subject>Antibodies, Blocking - pharmacology</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antigens, Neoplasm - biosynthesis</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Antigens, Surface - biosynthesis</subject><subject>Antigens, Surface - immunology</subject><subject>Apoptosis - immunology</subject><subject>Cell Aggregation - immunology</subject><subject>Cell Death - immunology</subject><subject>Cell Line, Transformed</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Fetus - cytology</subject><subject>Fetus - immunology</subject><subject>Fetus - metabolism</subject><subject>Glycosylphosphatidylinositols - metabolism</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Humans</subject><subject>Immunosuppressive Agents - immunology</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphoma, T-Cell</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Weight</subject><subject>Precipitin Tests</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - pathology</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkd9u0zAYRy0EYmXwBEjIV3CV4n9x0stQ2Ia0aROUa8txvrTenLjYDlGfhNedqw6xK1985_xk6SD0npKlIGL1-d4OwzR6t6SSL_mSciJfoAUtS1JISeRLtCCEsYJWsjpDb2K8J4RIwsRrdEaJoCVh9QL9bfCNH71xftQON2Oyre8O-Adok-wfwLNNO6yxIMXDV51RB2ZygP2ILyBlY7M7DN4cEkSsxw5vpsEHvAbnIv7ivHmI-C54Z3sIOtlsHaGfyQ6T00en2W4DbP-fmr3fJx9tfIte9dpFePf0nqNfF98266vi-vby-7q5Lowgq1T0LaNccCrqrjZ1y3grpYRW046wbtUBtPlaGs4k1ZWEiptairJiLZW6FV3Pz9HH0-4--N8TxKQGG03-vx7BT1HRitOa0TKD_ASa4GMM0Kt9sIMOB0WJOvZQ_3qo3ENxdeyRrQ9P81M7QPfMOQXIwKcTsLPb3WwDqDho5zJO1TzPz6YeAcqGmB4</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>Fernandes, Dancella M</creator><creator>Baird, Allison M</creator><creator>Berg, Leslie J</creator><creator>Rock, Kenneth L</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19990801</creationdate><title>A Monoclonal Antibody Reactive with a 40-kDa Molecule on Fetal Thymocytes and Tumor Cells Blocks Proliferation and Stimulates Aggregation and Apoptosis</title><author>Fernandes, Dancella M ; Baird, Allison M ; Berg, Leslie J ; Rock, Kenneth L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-fb21343148d8c8b23b666eba1d02d9deeb3435c3261a76e73c864572b16ab4df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibodies, Blocking - metabolism</topic><topic>Antibodies, Blocking - pharmacology</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Antigens, Neoplasm - biosynthesis</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Antigens, Surface - biosynthesis</topic><topic>Antigens, Surface - immunology</topic><topic>Apoptosis - immunology</topic><topic>Cell Aggregation - immunology</topic><topic>Cell Death - immunology</topic><topic>Cell Line, Transformed</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Fetus - cytology</topic><topic>Fetus - immunology</topic><topic>Fetus - metabolism</topic><topic>Glycosylphosphatidylinositols - metabolism</topic><topic>Hematopoietic Stem Cells - immunology</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Humans</topic><topic>Immunosuppressive Agents - immunology</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphoma, T-Cell</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Weight</topic><topic>Precipitin Tests</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - pathology</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandes, Dancella M</creatorcontrib><creatorcontrib>Baird, Allison M</creatorcontrib><creatorcontrib>Berg, Leslie J</creatorcontrib><creatorcontrib>Rock, Kenneth L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandes, Dancella M</au><au>Baird, Allison M</au><au>Berg, Leslie J</au><au>Rock, Kenneth L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Monoclonal Antibody Reactive with a 40-kDa Molecule on Fetal Thymocytes and Tumor Cells Blocks Proliferation and Stimulates Aggregation and Apoptosis</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>163</volume><issue>3</issue><spage>1306</spage><epage>1314</epage><pages>1306-1314</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>E710.2.3 is a murine thymic lymphoma cell line with an immature phenotype (CD4-CD8-) that proliferates in response to thymocytes or PMA when cultured at low density and proliferates spontaneously when grown at high density. 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The Ab immunoprecipitated a 40-kDa molecule from E710.2.3 that was not glycosylphosphatidylinositol linked. The data suggest that the molecule recognized by DMF62.3 is a novel cell surface molecule that may be involved in cell proliferation and/or cell death.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>10415028</pmid><doi>10.4049/jimmunol.163.3.1306</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
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subjects Animals
Antibodies, Blocking - metabolism
Antibodies, Blocking - pharmacology
Antibodies, Monoclonal - metabolism
Antibodies, Monoclonal - pharmacology
Antigens, Neoplasm - biosynthesis
Antigens, Neoplasm - immunology
Antigens, Surface - biosynthesis
Antigens, Surface - immunology
Apoptosis - immunology
Cell Aggregation - immunology
Cell Death - immunology
Cell Line, Transformed
Cricetinae
Cricetulus
Fetus - cytology
Fetus - immunology
Fetus - metabolism
Glycosylphosphatidylinositols - metabolism
Hematopoietic Stem Cells - immunology
Hematopoietic Stem Cells - metabolism
Humans
Immunosuppressive Agents - immunology
Immunosuppressive Agents - pharmacology
Lymphocyte Activation - drug effects
Lymphocyte Activation - immunology
Lymphoma, T-Cell
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Weight
Precipitin Tests
T-Lymphocytes - immunology
T-Lymphocytes - pathology
Tetradecanoylphorbol Acetate - pharmacology
Tumor Cells, Cultured
title A Monoclonal Antibody Reactive with a 40-kDa Molecule on Fetal Thymocytes and Tumor Cells Blocks Proliferation and Stimulates Aggregation and Apoptosis
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