Calretinin and parvalbumin immunoreactive interneurons in the retrosplenial cortex of the rat brain: Qualitative and quantitative analyses

Abstract The retrosplenial cortex (RSC) is a mesocortical region broadly involved with memory and navigation. It shares many characteristics with the perirhinal cortex (PRC), both of which appear to be significantly involved in the spreading of epileptic activity. We hypothesized that RSC possesses...

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Veröffentlicht in:Brain research 2015-11, Vol.1627, p.201-215
Hauptverfasser: Salaj, Martin, Druga, Rastislav, Cerman, Jiří, Kubová, Hana, Barinka, Filip
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Druga, Rastislav
Cerman, Jiří
Kubová, Hana
Barinka, Filip
description Abstract The retrosplenial cortex (RSC) is a mesocortical region broadly involved with memory and navigation. It shares many characteristics with the perirhinal cortex (PRC), both of which appear to be significantly involved in the spreading of epileptic activity. We hypothesized that RSC possesses an interneuronal composition similar to that of PRC. To prove the hypothesis we studied the general pattern of calretinin (CR) and parvalbumin (PV) immunoreactivity in the RSC of the rat brain, its optical density as well as the morphological features and density of CR- and PV-immunoreactive (CR+ and PV+) interneurons. We also analyzed the overall neuronal density on Nissl-stained sections in RSC. Finally, we compared our results with our earlier analysis of PRC ( Barinka et al., 2012 ). Compared to PRC, RSC was observed to have a higher intensity of PV staining and lower intensity of CR staining of neuropil. Vertically-oriented bipolar neurons were the most common morphological type among CR+ neurons. The staining pattern did not allow for a similarly detailed analysis of somatodendritic morphology of PV+ neurons. RSC possessed lower absolute (i.e., neurons/mm3 ) and relative (i.e., percentage of the overall neuronal population) densities of CR+ neurons and similar absolute and lower relative densities of PV+ neurons relative to PRC. CR: PV neuronal ratio in RSC (1:2 in area 29 and 1:2.2 in area 30) differed from PRC (1:1.2 in area 35 and 1:1.7 in area 36). In conclusion, RSC, although similar in many aspects to PRC, differs strikingly in the interneuronal composition relative to PRC.
doi_str_mv 10.1016/j.brainres.2015.09.031
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It shares many characteristics with the perirhinal cortex (PRC), both of which appear to be significantly involved in the spreading of epileptic activity. We hypothesized that RSC possesses an interneuronal composition similar to that of PRC. To prove the hypothesis we studied the general pattern of calretinin (CR) and parvalbumin (PV) immunoreactivity in the RSC of the rat brain, its optical density as well as the morphological features and density of CR- and PV-immunoreactive (CR+ and PV+) interneurons. We also analyzed the overall neuronal density on Nissl-stained sections in RSC. Finally, we compared our results with our earlier analysis of PRC ( Barinka et al., 2012 ). Compared to PRC, RSC was observed to have a higher intensity of PV staining and lower intensity of CR staining of neuropil. Vertically-oriented bipolar neurons were the most common morphological type among CR+ neurons. The staining pattern did not allow for a similarly detailed analysis of somatodendritic morphology of PV+ neurons. RSC possessed lower absolute (i.e., neurons/mm3 ) and relative (i.e., percentage of the overall neuronal population) densities of CR+ neurons and similar absolute and lower relative densities of PV+ neurons relative to PRC. CR: PV neuronal ratio in RSC (1:2 in area 29 and 1:2.2 in area 30) differed from PRC (1:1.2 in area 35 and 1:1.7 in area 36). 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The staining pattern did not allow for a similarly detailed analysis of somatodendritic morphology of PV+ neurons. RSC possessed lower absolute (i.e., neurons/mm3 ) and relative (i.e., percentage of the overall neuronal population) densities of CR+ neurons and similar absolute and lower relative densities of PV+ neurons relative to PRC. CR: PV neuronal ratio in RSC (1:2 in area 29 and 1:2.2 in area 30) differed from PRC (1:1.2 in area 35 and 1:1.7 in area 36). In conclusion, RSC, although similar in many aspects to PRC, differs strikingly in the interneuronal composition relative to PRC.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Calbindin 2 - metabolism</subject><subject>Calcium-binding proteins</subject><subject>Calretinin</subject><subject>Cerebral Cortex - cytology</subject><subject>Densitometry</subject><subject>Interneurons</subject><subject>Interneurons - metabolism</subject><subject>Male</subject><subject>Neurology</subject><subject>Parvalbumin</subject><subject>Parvalbumins - metabolism</subject><subject>Perirhinal cortex</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Retrosplenial cortex</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUsuOFCEUJUbjtKO_MGHppkoeVRS4MJqOr2QSY9Q1oalbkZaCHqA69i_41VLTM5q4cUUunAf3novQFSUtJVS82Le7ZFxIkFtGaN8S1RJOH6ANlQNrBOvIQ7QhhIhGKsUv0JOc97XkXJHH6IKJrlNC9hv0a2t8guKCC9iEER9MOhq_W-Zau3leQkxgbHFHwC4USAGWFEOuBS7fAVdqivngITjjsY2pwE8cp_ObKfj2ky_x58V4V8ytzOpys5hQ_l4Yf8qQn6JHk_EZnt2dl-jbu7dftx-a60_vP27fXDe2Y7w0vBPCAp9oN05CDEYJ6K2cYJKT7DkQ2yuuJBuZUr3aMS6tGW0vQapRENURfomen3UPKd4skIueXbbgvQkQl6zpwOmgqJBDhYoz1NYuc4JJH5KbTTppSvSag97r-xz0moMmStccKvHqzmPZzTD-od0PvgJenwFQOz06SDpbB8HC6BLYosfo_u_x6h8J62uO1vgfcIK8j0uqk6396Mw00V_WbViXgfaEDEz1_DfXwbWo</recordid><startdate>20151119</startdate><enddate>20151119</enddate><creator>Salaj, Martin</creator><creator>Druga, Rastislav</creator><creator>Cerman, Jiří</creator><creator>Kubová, Hana</creator><creator>Barinka, Filip</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3947-8522</orcidid></search><sort><creationdate>20151119</creationdate><title>Calretinin and parvalbumin immunoreactive interneurons in the retrosplenial cortex of the rat brain: Qualitative and quantitative analyses</title><author>Salaj, Martin ; Druga, Rastislav ; Cerman, Jiří ; Kubová, Hana ; Barinka, Filip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-3466ce3f14df667a96e5c8fef8f853e0c593982d29959b238cadc58e89d609403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Calbindin 2 - metabolism</topic><topic>Calcium-binding proteins</topic><topic>Calretinin</topic><topic>Cerebral Cortex - cytology</topic><topic>Densitometry</topic><topic>Interneurons</topic><topic>Interneurons - metabolism</topic><topic>Male</topic><topic>Neurology</topic><topic>Parvalbumin</topic><topic>Parvalbumins - metabolism</topic><topic>Perirhinal cortex</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Retrosplenial cortex</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salaj, Martin</creatorcontrib><creatorcontrib>Druga, Rastislav</creatorcontrib><creatorcontrib>Cerman, Jiří</creatorcontrib><creatorcontrib>Kubová, Hana</creatorcontrib><creatorcontrib>Barinka, Filip</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salaj, Martin</au><au>Druga, Rastislav</au><au>Cerman, Jiří</au><au>Kubová, Hana</au><au>Barinka, Filip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calretinin and parvalbumin immunoreactive interneurons in the retrosplenial cortex of the rat brain: Qualitative and quantitative analyses</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2015-11-19</date><risdate>2015</risdate><volume>1627</volume><spage>201</spage><epage>215</epage><pages>201-215</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>Abstract The retrosplenial cortex (RSC) is a mesocortical region broadly involved with memory and navigation. 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subjects Analysis of Variance
Animals
Calbindin 2 - metabolism
Calcium-binding proteins
Calretinin
Cerebral Cortex - cytology
Densitometry
Interneurons
Interneurons - metabolism
Male
Neurology
Parvalbumin
Parvalbumins - metabolism
Perirhinal cortex
Rats
Rats, Wistar
Retrosplenial cortex
title Calretinin and parvalbumin immunoreactive interneurons in the retrosplenial cortex of the rat brain: Qualitative and quantitative analyses
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