Voltage-gated, margatoxin-sensitive potassium channels, but not calcium-gated, iberiotoxin-sensitive potassium channels modulate acetylcholine release in rat striatal slices
We evaluated the effects of iberiotoxin, an inhibitor of Slo-type Ca 2+-activated potassium channels and two inhibitors of Shaker-type voltage-gated potassium channels margatoxin and dendrotoxin on acetylcholine outflow in rat striatal slices. An in vitro perfusion with 100 nM margatoxin or dendroto...
Gespeichert in:
| Veröffentlicht in: | Neuroscience letters 1999-03, Vol.263 (2), p.208-210 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 210 |
|---|---|
| container_issue | 2 |
| container_start_page | 208 |
| container_title | Neuroscience letters |
| container_volume | 263 |
| creator | Fischer, Hanspeter S Saria, Alois |
| description | We evaluated the effects of iberiotoxin, an inhibitor of Slo-type Ca
2+-activated potassium channels and two inhibitors of Shaker-type voltage-gated potassium channels margatoxin and dendrotoxin on acetylcholine outflow in rat striatal slices. An in vitro perfusion with 100 nM margatoxin or dendrotoxin induced a concentration-dependent and tetrodotoxin-sensitive enhancement in spontaneous acetylcholine release. In contrast, a perfusion with iberiotoxin did neither modulate basal, nor electrically- or
N-methyl-
d-aspartate-induced transmitter release. Therefore, Slo-type Ca
2+-activated K
+-channels do not seem to contribute significantly to cholinergic neurotransmission within rat striatal slices. As the Kv1.2 subtype represents the only common high affinity binding site of margatoxin and dendrotoxin and the effects of these toxins are not additive, this subtype is suggested to be the channel utilized by margatoxin and dendrotoxin to release acetylcholine in this model. |
| doi_str_mv | 10.1016/S0304-3940(99)00116-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17313958</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0304394099001160</els_id><sourcerecordid>17313958</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-9e643e95111ce1781774ce55bfe88ed582bd8b63aa3c2f40c02d3cc5c6590dba3</originalsourceid><addsrcrecordid>eNqFkctu1TAQhi0EoqeFRwB5gVCRGrDjOIlXCFXcpEosuGwtZzJpjRz74HEq-lC8I-k5h8uuK1vy949H_8fYEyleSiHbV5-FEk2lTCNOjXkhhJRtJe6xjey7uupMV99nm7_IETsm-i6E0FI3D9mRFLVUsqs37Ne3FIq7xOrSFRzP-Ozyeks_fawII_nir5FvU3FEfpk5XLkYMdAZH5bCYyocXID15U_eD5h9unsAn9O4hDXDHWC5CXCVgo_IMwZ0hNxHnl3hVLJ3xQVOwQPSI_ZgcoHw8eE8YV_fvf1y_qG6-PT-4_mbiwqaWpbKYNsoNFpKCSi7XnZdA6j1MGHf46j7ehj7oVXOKainRoCoRwWgodVGjINTJ-z5fu42px8LUrGzJ8AQXMS0kJWdksrofgX1HoSciDJOdpv92uGNlcLeerI7T_ZWgjXG7jxZseaeHj5YhhnH_1J7MSvw7AA4Wiuesovg6R_XNU2_w17vsbVSvPaYLYHHCDj6jFDsmPwdm_wGX-y0mA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17313958</pqid></control><display><type>article</type><title>Voltage-gated, margatoxin-sensitive potassium channels, but not calcium-gated, iberiotoxin-sensitive potassium channels modulate acetylcholine release in rat striatal slices</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Fischer, Hanspeter S ; Saria, Alois</creator><creatorcontrib>Fischer, Hanspeter S ; Saria, Alois</creatorcontrib><description>We evaluated the effects of iberiotoxin, an inhibitor of Slo-type Ca
2+-activated potassium channels and two inhibitors of Shaker-type voltage-gated potassium channels margatoxin and dendrotoxin on acetylcholine outflow in rat striatal slices. An in vitro perfusion with 100 nM margatoxin or dendrotoxin induced a concentration-dependent and tetrodotoxin-sensitive enhancement in spontaneous acetylcholine release. In contrast, a perfusion with iberiotoxin did neither modulate basal, nor electrically- or
N-methyl-
d-aspartate-induced transmitter release. Therefore, Slo-type Ca
2+-activated K
+-channels do not seem to contribute significantly to cholinergic neurotransmission within rat striatal slices. As the Kv1.2 subtype represents the only common high affinity binding site of margatoxin and dendrotoxin and the effects of these toxins are not additive, this subtype is suggested to be the channel utilized by margatoxin and dendrotoxin to release acetylcholine in this model.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(99)00116-0</identifier><identifier>PMID: 10213172</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Acetylcholine - metabolism ; Acetylcholine release ; Animals ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Corpus Striatum - drug effects ; Corpus Striatum - physiology ; Dendrotoxin ; Elapid Venoms - pharmacology ; Fundamental and applied biological sciences. Psychology ; Iberiotoxin ; In vitro perfusion ; In Vitro Techniques ; Kv1.2 Potassium Channel ; Large-Conductance Calcium-Activated Potassium Channel alpha Subunits ; Large-Conductance Calcium-Activated Potassium Channels ; Margatoxin ; N-Methylaspartate - pharmacology ; Neurotoxins - pharmacology ; Peptides - pharmacology ; Potassium channels ; Potassium Channels - drug effects ; Potassium Channels - physiology ; Potassium Channels, Calcium-Activated ; Potassium Channels, Voltage-Gated ; Rats ; Scorpion Venoms - pharmacology ; Shaker Superfamily of Potassium Channels ; Striatum slices ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 1999-03, Vol.263 (2), p.208-210</ispartof><rights>1999 Elsevier Science Ireland Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-9e643e95111ce1781774ce55bfe88ed582bd8b63aa3c2f40c02d3cc5c6590dba3</citedby><cites>FETCH-LOGICAL-c421t-9e643e95111ce1781774ce55bfe88ed582bd8b63aa3c2f40c02d3cc5c6590dba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304394099001160$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1744872$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10213172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fischer, Hanspeter S</creatorcontrib><creatorcontrib>Saria, Alois</creatorcontrib><title>Voltage-gated, margatoxin-sensitive potassium channels, but not calcium-gated, iberiotoxin-sensitive potassium channels modulate acetylcholine release in rat striatal slices</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>We evaluated the effects of iberiotoxin, an inhibitor of Slo-type Ca
2+-activated potassium channels and two inhibitors of Shaker-type voltage-gated potassium channels margatoxin and dendrotoxin on acetylcholine outflow in rat striatal slices. An in vitro perfusion with 100 nM margatoxin or dendrotoxin induced a concentration-dependent and tetrodotoxin-sensitive enhancement in spontaneous acetylcholine release. In contrast, a perfusion with iberiotoxin did neither modulate basal, nor electrically- or
N-methyl-
d-aspartate-induced transmitter release. Therefore, Slo-type Ca
2+-activated K
+-channels do not seem to contribute significantly to cholinergic neurotransmission within rat striatal slices. As the Kv1.2 subtype represents the only common high affinity binding site of margatoxin and dendrotoxin and the effects of these toxins are not additive, this subtype is suggested to be the channel utilized by margatoxin and dendrotoxin to release acetylcholine in this model.</description><subject>Acetylcholine - metabolism</subject><subject>Acetylcholine release</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - physiology</subject><subject>Dendrotoxin</subject><subject>Elapid Venoms - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Iberiotoxin</subject><subject>In vitro perfusion</subject><subject>In Vitro Techniques</subject><subject>Kv1.2 Potassium Channel</subject><subject>Large-Conductance Calcium-Activated Potassium Channel alpha Subunits</subject><subject>Large-Conductance Calcium-Activated Potassium Channels</subject><subject>Margatoxin</subject><subject>N-Methylaspartate - pharmacology</subject><subject>Neurotoxins - pharmacology</subject><subject>Peptides - pharmacology</subject><subject>Potassium channels</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - physiology</subject><subject>Potassium Channels, Calcium-Activated</subject><subject>Potassium Channels, Voltage-Gated</subject><subject>Rats</subject><subject>Scorpion Venoms - pharmacology</subject><subject>Shaker Superfamily of Potassium Channels</subject><subject>Striatum slices</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAQhi0EoqeFRwB5gVCRGrDjOIlXCFXcpEosuGwtZzJpjRz74HEq-lC8I-k5h8uuK1vy949H_8fYEyleSiHbV5-FEk2lTCNOjXkhhJRtJe6xjey7uupMV99nm7_IETsm-i6E0FI3D9mRFLVUsqs37Ne3FIq7xOrSFRzP-Ozyeks_fawII_nir5FvU3FEfpk5XLkYMdAZH5bCYyocXID15U_eD5h9unsAn9O4hDXDHWC5CXCVgo_IMwZ0hNxHnl3hVLJ3xQVOwQPSI_ZgcoHw8eE8YV_fvf1y_qG6-PT-4_mbiwqaWpbKYNsoNFpKCSi7XnZdA6j1MGHf46j7ehj7oVXOKainRoCoRwWgodVGjINTJ-z5fu42px8LUrGzJ8AQXMS0kJWdksrofgX1HoSciDJOdpv92uGNlcLeerI7T_ZWgjXG7jxZseaeHj5YhhnH_1J7MSvw7AA4Wiuesovg6R_XNU2_w17vsbVSvPaYLYHHCDj6jFDsmPwdm_wGX-y0mA</recordid><startdate>19990326</startdate><enddate>19990326</enddate><creator>Fischer, Hanspeter S</creator><creator>Saria, Alois</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19990326</creationdate><title>Voltage-gated, margatoxin-sensitive potassium channels, but not calcium-gated, iberiotoxin-sensitive potassium channels modulate acetylcholine release in rat striatal slices</title><author>Fischer, Hanspeter S ; Saria, Alois</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-9e643e95111ce1781774ce55bfe88ed582bd8b63aa3c2f40c02d3cc5c6590dba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acetylcholine - metabolism</topic><topic>Acetylcholine release</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - physiology</topic><topic>Dendrotoxin</topic><topic>Elapid Venoms - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Iberiotoxin</topic><topic>In vitro perfusion</topic><topic>In Vitro Techniques</topic><topic>Kv1.2 Potassium Channel</topic><topic>Large-Conductance Calcium-Activated Potassium Channel alpha Subunits</topic><topic>Large-Conductance Calcium-Activated Potassium Channels</topic><topic>Margatoxin</topic><topic>N-Methylaspartate - pharmacology</topic><topic>Neurotoxins - pharmacology</topic><topic>Peptides - pharmacology</topic><topic>Potassium channels</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>Potassium Channels, Calcium-Activated</topic><topic>Potassium Channels, Voltage-Gated</topic><topic>Rats</topic><topic>Scorpion Venoms - pharmacology</topic><topic>Shaker Superfamily of Potassium Channels</topic><topic>Striatum slices</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fischer, Hanspeter S</creatorcontrib><creatorcontrib>Saria, Alois</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fischer, Hanspeter S</au><au>Saria, Alois</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Voltage-gated, margatoxin-sensitive potassium channels, but not calcium-gated, iberiotoxin-sensitive potassium channels modulate acetylcholine release in rat striatal slices</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1999-03-26</date><risdate>1999</risdate><volume>263</volume><issue>2</issue><spage>208</spage><epage>210</epage><pages>208-210</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>We evaluated the effects of iberiotoxin, an inhibitor of Slo-type Ca
2+-activated potassium channels and two inhibitors of Shaker-type voltage-gated potassium channels margatoxin and dendrotoxin on acetylcholine outflow in rat striatal slices. An in vitro perfusion with 100 nM margatoxin or dendrotoxin induced a concentration-dependent and tetrodotoxin-sensitive enhancement in spontaneous acetylcholine release. In contrast, a perfusion with iberiotoxin did neither modulate basal, nor electrically- or
N-methyl-
d-aspartate-induced transmitter release. Therefore, Slo-type Ca
2+-activated K
+-channels do not seem to contribute significantly to cholinergic neurotransmission within rat striatal slices. As the Kv1.2 subtype represents the only common high affinity binding site of margatoxin and dendrotoxin and the effects of these toxins are not additive, this subtype is suggested to be the channel utilized by margatoxin and dendrotoxin to release acetylcholine in this model.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>10213172</pmid><doi>10.1016/S0304-3940(99)00116-0</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1999-03, Vol.263 (2), p.208-210 |
| issn | 0304-3940 1872-7972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17313958 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetylcholine - metabolism Acetylcholine release Animals Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Corpus Striatum - drug effects Corpus Striatum - physiology Dendrotoxin Elapid Venoms - pharmacology Fundamental and applied biological sciences. Psychology Iberiotoxin In vitro perfusion In Vitro Techniques Kv1.2 Potassium Channel Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Large-Conductance Calcium-Activated Potassium Channels Margatoxin N-Methylaspartate - pharmacology Neurotoxins - pharmacology Peptides - pharmacology Potassium channels Potassium Channels - drug effects Potassium Channels - physiology Potassium Channels, Calcium-Activated Potassium Channels, Voltage-Gated Rats Scorpion Venoms - pharmacology Shaker Superfamily of Potassium Channels Striatum slices Vertebrates: nervous system and sense organs |
| title | Voltage-gated, margatoxin-sensitive potassium channels, but not calcium-gated, iberiotoxin-sensitive potassium channels modulate acetylcholine release in rat striatal slices |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T23%3A29%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Voltage-gated,%20margatoxin-sensitive%20potassium%20channels,%20but%20not%20calcium-gated,%20iberiotoxin-sensitive%20potassium%20channels%20modulate%20acetylcholine%20release%20in%20rat%20striatal%20slices&rft.jtitle=Neuroscience%20letters&rft.au=Fischer,%20Hanspeter%20S&rft.date=1999-03-26&rft.volume=263&rft.issue=2&rft.spage=208&rft.epage=210&rft.pages=208-210&rft.issn=0304-3940&rft.eissn=1872-7972&rft.coden=NELED5&rft_id=info:doi/10.1016/S0304-3940(99)00116-0&rft_dat=%3Cproquest_cross%3E17313958%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17313958&rft_id=info:pmid/10213172&rft_els_id=S0304394099001160&rfr_iscdi=true |