Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera)
Two sets of PCR primers were designed from DNA sequence which flanked the 3′ end of thePyrenopeziza brassicae mating type idiomorphs (MAT-1 and MAT-2). The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of 50 isolates of P. brassicae , which included both mat...
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| Veröffentlicht in: | Physiological and molecular plant pathology 1999-08, Vol.55 (2), p.111-119 |
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| creator | FOSTER, S.J SINGH, G FITT, B.D.L ASHBY, A.M |
| description | Two sets of PCR primers were designed from DNA sequence which flanked the 3′ end of thePyrenopeziza brassicae mating type idiomorphs (MAT-1 and MAT-2). The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of 50 isolates of P. brassicae , which included both mating types. The 750 bp PCR product was also amplified from DNA extracted from Brassica napus tissue infected with P. brassicae . The 750 bp PCR product was not produced when these diagnostic primers were used in PCR reactions containing DNA from a number of other fungal species, including fungal pathogens of oilseed rape and Tapesia yallundae , which is closely related to P. brassicae . The second set of primers were designed to amplify different sized products from the two mating types of P. brassicae . These mating type specific primers facilitated the amplification of a 440 bp product from all MAT-1 isolates tested and a 535 bp product from 30% of the MAT-2 isolates tested. The value of molecular diagnostic techniques for the identification of P. brassicae in oilseed rape crops, for distinguishing between mating types of the fungus and in disease forecasting is discussed. |
| doi_str_mv | 10.1006/pmpp.1999.0207 |
| format | Article |
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The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of 50 isolates of P. brassicae , which included both mating types. The 750 bp PCR product was also amplified from DNA extracted from Brassica napus tissue infected with P. brassicae . The 750 bp PCR product was not produced when these diagnostic primers were used in PCR reactions containing DNA from a number of other fungal species, including fungal pathogens of oilseed rape and Tapesia yallundae , which is closely related to P. brassicae . The second set of primers were designed to amplify different sized products from the two mating types of P. brassicae . These mating type specific primers facilitated the amplification of a 440 bp product from all MAT-1 isolates tested and a 535 bp product from 30% of the MAT-2 isolates tested. The value of molecular diagnostic techniques for the identification of P. brassicae in oilseed rape crops, for distinguishing between mating types of the fungus and in disease forecasting is discussed.</description><identifier>ISSN: 0885-5765</identifier><identifier>EISSN: 1096-1178</identifier><identifier>DOI: 10.1006/pmpp.1999.0207</identifier><identifier>CODEN: PMPPEZ</identifier><language>eng</language><publisher>London: Elsevier India Pvt Ltd</publisher><subject>Alternaria brassicae ; Biological and medical sciences ; Botrytis cinerea ; Brassica napus ; Brassica napus ssp. oleifera ; Brassica napus var. napus ; diagnosis ; diagnostic techniques ; disease diagnosis ; DNA ; DNA primers ; Fundamental and applied biological sciences. Psychology ; fungal diseases of plants ; Fungal plant pathogens ; Fusarium oxysporum ; Generalities. Techniques ; identification ; infection ; leaf spotting ; Leptosphaeria maculans ; mating type ; molecular sequence data ; Nectria haematococca ; Neurospora crassa ; nucleotide sequences ; PCR ; Phytopathology. Animal pests. Plant and forest protection ; polymerase chain reaction ; Pyrenopeziza brassicae ; Sclerotinia sclerotiorum ; Tapesia yallundae ; Verticillium dahliae</subject><ispartof>Physiological and molecular plant pathology, 1999-08, Vol.55 (2), p.111-119</ispartof><rights>1999 Academic Press</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-4805bc372ee83cdc87f5bf93f1868e10d776088d92ecdeeb0da32fb31388bd193</citedby><cites>FETCH-LOGICAL-c370t-4805bc372ee83cdc87f5bf93f1868e10d776088d92ecdeeb0da32fb31388bd193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/pmpp.1999.0207$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1902648$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>FOSTER, S.J</creatorcontrib><creatorcontrib>SINGH, G</creatorcontrib><creatorcontrib>FITT, B.D.L</creatorcontrib><creatorcontrib>ASHBY, A.M</creatorcontrib><title>Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera)</title><title>Physiological and molecular plant pathology</title><description>Two sets of PCR primers were designed from DNA sequence which flanked the 3′ end of thePyrenopeziza brassicae mating type idiomorphs (MAT-1 and MAT-2). The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of 50 isolates of P. brassicae , which included both mating types. The 750 bp PCR product was also amplified from DNA extracted from Brassica napus tissue infected with P. brassicae . The 750 bp PCR product was not produced when these diagnostic primers were used in PCR reactions containing DNA from a number of other fungal species, including fungal pathogens of oilseed rape and Tapesia yallundae , which is closely related to P. brassicae . The second set of primers were designed to amplify different sized products from the two mating types of P. brassicae . These mating type specific primers facilitated the amplification of a 440 bp product from all MAT-1 isolates tested and a 535 bp product from 30% of the MAT-2 isolates tested. The value of molecular diagnostic techniques for the identification of P. brassicae in oilseed rape crops, for distinguishing between mating types of the fungus and in disease forecasting is discussed.</description><subject>Alternaria brassicae</subject><subject>Biological and medical sciences</subject><subject>Botrytis cinerea</subject><subject>Brassica napus</subject><subject>Brassica napus ssp. oleifera</subject><subject>Brassica napus var. napus</subject><subject>diagnosis</subject><subject>diagnostic techniques</subject><subject>disease diagnosis</subject><subject>DNA</subject><subject>DNA primers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal diseases of plants</subject><subject>Fungal plant pathogens</subject><subject>Fusarium oxysporum</subject><subject>Generalities. Techniques</subject><subject>identification</subject><subject>infection</subject><subject>leaf spotting</subject><subject>Leptosphaeria maculans</subject><subject>mating type</subject><subject>molecular sequence data</subject><subject>Nectria haematococca</subject><subject>Neurospora crassa</subject><subject>nucleotide sequences</subject><subject>PCR</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>polymerase chain reaction</subject><subject>Pyrenopeziza brassicae</subject><subject>Sclerotinia sclerotiorum</subject><subject>Tapesia yallundae</subject><subject>Verticillium dahliae</subject><issn>0885-5765</issn><issn>1096-1178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNp1kU2LFDEQhhtRcFy9erUOIu6h26Q_k6OO7iosKOqeQzpdmYl0JzHJ7DL7j_yXppkBT56qoJ63vt6ieElJRQnp3_nF-4pyzitSk-FRsaGE9yWlA3tcbAhjXdkNffe0eBbjL0IIbyndFH8-4h3Ozi9oEzgN37bfYZQRJ5iM3FkXk1GQUO2t-X3ACNoFSHuEdO9gkcnYHaSjz4VVewxonccH8yBhDDJGoyTC29ns9glmlBqid-kSnIV7YxMGcGaOmIcF6TP44awBK_0hQoy-Ajej0Rjk5fPiiZaZfnGOF8Xt1aef28_lzdfrL9v3N6VqBpLKlpFuzGmNyBo1KTbobtS80ZT1DCmZhqHPz5h4jWpCHMkkm1qPDW0YGyfKm4vizamvD249OYnFRIXzLC26QxR0yGzd9hmsTqAKLsaAWvhgFhmOghKxOiJWR8TqiFgdyYLX584yKjnrIK0y8Z-Kk7pvWcZenTAtnZC7kJHbHzWhDal5W3PWZIKdCMx_uDMYRFQGrcLJBFRJTM78b4e_3D2r2A</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>FOSTER, S.J</creator><creator>SINGH, G</creator><creator>FITT, B.D.L</creator><creator>ASHBY, A.M</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19990801</creationdate><title>Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera)</title><author>FOSTER, S.J ; SINGH, G ; FITT, B.D.L ; ASHBY, A.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-4805bc372ee83cdc87f5bf93f1868e10d776088d92ecdeeb0da32fb31388bd193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Alternaria brassicae</topic><topic>Biological and medical sciences</topic><topic>Botrytis cinerea</topic><topic>Brassica napus</topic><topic>Brassica napus ssp. oleifera</topic><topic>Brassica napus var. napus</topic><topic>diagnosis</topic><topic>diagnostic techniques</topic><topic>disease diagnosis</topic><topic>DNA</topic><topic>DNA primers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal diseases of plants</topic><topic>Fungal plant pathogens</topic><topic>Fusarium oxysporum</topic><topic>Generalities. Techniques</topic><topic>identification</topic><topic>infection</topic><topic>leaf spotting</topic><topic>Leptosphaeria maculans</topic><topic>mating type</topic><topic>molecular sequence data</topic><topic>Nectria haematococca</topic><topic>Neurospora crassa</topic><topic>nucleotide sequences</topic><topic>PCR</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>polymerase chain reaction</topic><topic>Pyrenopeziza brassicae</topic><topic>Sclerotinia sclerotiorum</topic><topic>Tapesia yallundae</topic><topic>Verticillium dahliae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FOSTER, S.J</creatorcontrib><creatorcontrib>SINGH, G</creatorcontrib><creatorcontrib>FITT, B.D.L</creatorcontrib><creatorcontrib>ASHBY, A.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Physiological and molecular plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FOSTER, S.J</au><au>SINGH, G</au><au>FITT, B.D.L</au><au>ASHBY, A.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera)</atitle><jtitle>Physiological and molecular plant pathology</jtitle><date>1999-08-01</date><risdate>1999</risdate><volume>55</volume><issue>2</issue><spage>111</spage><epage>119</epage><pages>111-119</pages><issn>0885-5765</issn><eissn>1096-1178</eissn><coden>PMPPEZ</coden><abstract>Two sets of PCR primers were designed from DNA sequence which flanked the 3′ end of thePyrenopeziza brassicae mating type idiomorphs (MAT-1 and MAT-2). The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of 50 isolates of P. brassicae , which included both mating types. The 750 bp PCR product was also amplified from DNA extracted from Brassica napus tissue infected with P. brassicae . The 750 bp PCR product was not produced when these diagnostic primers were used in PCR reactions containing DNA from a number of other fungal species, including fungal pathogens of oilseed rape and Tapesia yallundae , which is closely related to P. brassicae . The second set of primers were designed to amplify different sized products from the two mating types of P. brassicae . These mating type specific primers facilitated the amplification of a 440 bp product from all MAT-1 isolates tested and a 535 bp product from 30% of the MAT-2 isolates tested. The value of molecular diagnostic techniques for the identification of P. brassicae in oilseed rape crops, for distinguishing between mating types of the fungus and in disease forecasting is discussed.</abstract><cop>London</cop><pub>Elsevier India Pvt Ltd</pub><doi>10.1006/pmpp.1999.0207</doi><tpages>9</tpages></addata></record> |
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| subjects | Alternaria brassicae Biological and medical sciences Botrytis cinerea Brassica napus Brassica napus ssp. oleifera Brassica napus var. napus diagnosis diagnostic techniques disease diagnosis DNA DNA primers Fundamental and applied biological sciences. Psychology fungal diseases of plants Fungal plant pathogens Fusarium oxysporum Generalities. Techniques identification infection leaf spotting Leptosphaeria maculans mating type molecular sequence data Nectria haematococca Neurospora crassa nucleotide sequences PCR Phytopathology. Animal pests. Plant and forest protection polymerase chain reaction Pyrenopeziza brassicae Sclerotinia sclerotiorum Tapesia yallundae Verticillium dahliae |
| title | Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera) |
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