Noninvasive NMR studies of metabolism in cultured Catharanthus roseus cells
Nuclear magnetic resonance (NMR) spectroscopy provides a unique modality for the study of tissue-cultured plant cells. One of its major attractions is that it allows noninvasive studies of plant material. In addition, it can provide insight into the pH in the vacuole and cytoplasm, and into the comp...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 1999-03, Vol.35 (2), p.144-151 |
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| container_title | In vitro cellular & developmental biology. Plant |
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| creator | Vogel, H.J Lundberg, P Bagh, K |
| description | Nuclear magnetic resonance (NMR) spectroscopy provides a unique modality for the study of tissue-cultured plant cells. One of its major attractions is that it allows noninvasive studies of plant material. In addition, it can provide insight into the pH in the vacuole and cytoplasm, and into the compartmentalization of certain metabolites. In this review we show how phosphorus-31 NMR is used to study intracellular pH, phosphate uptake and storage, and energy metabolism in suspension cells of Catharanthus roseus. In addition, multinuclear NMR studies of the uptake of ammonium and the gradients of K+over the membrane are discussed as well. The use of two-dimensional NMR for the study of whole cell extracts is also described. Finally, we show how nitrogen-14 and nitrogen-15 NMR are used to obtain information about the assimilation of inorganic sources in developing carrot somatic embryos. These NMR studies provide a unique insight into the metabolism of tissue-cultured plant cells. |
| doi_str_mv | 10.1007/s11627-999-0024-y |
| format | Article |
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One of its major attractions is that it allows noninvasive studies of plant material. In addition, it can provide insight into the pH in the vacuole and cytoplasm, and into the compartmentalization of certain metabolites. In this review we show how phosphorus-31 NMR is used to study intracellular pH, phosphate uptake and storage, and energy metabolism in suspension cells of Catharanthus roseus. In addition, multinuclear NMR studies of the uptake of ammonium and the gradients of K+over the membrane are discussed as well. The use of two-dimensional NMR for the study of whole cell extracts is also described. Finally, we show how nitrogen-14 and nitrogen-15 NMR are used to obtain information about the assimilation of inorganic sources in developing carrot somatic embryos. These NMR studies provide a unique insight into the metabolism of tissue-cultured plant cells.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-999-0024-y</identifier><language>eng</language><publisher>Wallingford: Society for In Vitro Biology</publisher><subject>Agronomy. Soil science and plant productions ; Amino acid metabolism ; ammonium compounds ; Biological and medical sciences ; Biotechnology ; Catharanthus roseus ; Cell extracts ; cell suspension culture ; Cellular metabolism ; Cultured cells ; Cytoplasm ; Economic plant physiology ; Energy metabolism ; Fundamental and applied biological sciences. Psychology ; glutamate-ammonia ligase ; In vitro culture ; metabolism ; metabolites ; Nuclear magnetic resonance ; nuclear magnetic resonance spectroscopy ; nutrient reserves ; nutrient uptake ; phosphates ; Plant cells ; Plant physiology and development ; Plants ; Tissue cultures, protoplasts ; Vacuoles</subject><ispartof>In vitro cellular & developmental biology. 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Plant</title><description>Nuclear magnetic resonance (NMR) spectroscopy provides a unique modality for the study of tissue-cultured plant cells. One of its major attractions is that it allows noninvasive studies of plant material. In addition, it can provide insight into the pH in the vacuole and cytoplasm, and into the compartmentalization of certain metabolites. In this review we show how phosphorus-31 NMR is used to study intracellular pH, phosphate uptake and storage, and energy metabolism in suspension cells of Catharanthus roseus. In addition, multinuclear NMR studies of the uptake of ammonium and the gradients of K+over the membrane are discussed as well. The use of two-dimensional NMR for the study of whole cell extracts is also described. Finally, we show how nitrogen-14 and nitrogen-15 NMR are used to obtain information about the assimilation of inorganic sources in developing carrot somatic embryos. These NMR studies provide a unique insight into the metabolism of tissue-cultured plant cells.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino acid metabolism</subject><subject>ammonium compounds</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Catharanthus roseus</subject><subject>Cell extracts</subject><subject>cell suspension culture</subject><subject>Cellular metabolism</subject><subject>Cultured cells</subject><subject>Cytoplasm</subject><subject>Economic plant physiology</subject><subject>Energy metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glutamate-ammonia ligase</subject><subject>In vitro culture</subject><subject>metabolism</subject><subject>metabolites</subject><subject>Nuclear magnetic resonance</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>nutrient reserves</subject><subject>nutrient uptake</subject><subject>phosphates</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Tissue cultures, protoplasts</subject><subject>Vacuoles</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNpFkEFr3DAQhU1Jocm2P6BQqA-hNyczsmxJx7KkTcgmgbR7FmNZahy8dqKxA_vvq8UhOb2B981j5mXZV4QzBFDnjFgLVRhjCgAhi_2H7BilqgpRa3OUZqhkUUlVf8pOmB8BAAHVcXZ9Ow7d8ELcvfj89uY-52luO8_5GPKdn6gZ-453eTfkbu6nOfo2X9P0QJGG6WHmPI7skzjf9_w5-xioZ__lVVfZ9tfF3_Vlsbn7fbX-uSlcKfVUBNCgGulQq1Y30BrhsUXSAcgbhVQZWVHdVLqtJZTGeeGJgtBGkwuqMeUq-7HkPsXxefY82V3Hhwto8OPMFlWJUCuRQFxAl87k6IN9it2O4t4i2ENtdqnNptrsoTa7Tzunr-HEjvqQHnUdvy9qaZRWCfu2YI88jfHNlsKUqOpkf1_sQKOlfzElbP8IwBISoGppyv9iYYC_</recordid><startdate>19990301</startdate><enddate>19990301</enddate><creator>Vogel, H.J</creator><creator>Lundberg, P</creator><creator>Bagh, K</creator><general>Society for In Vitro Biology</general><general>Cambridge University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19990301</creationdate><title>Noninvasive NMR studies of metabolism in cultured Catharanthus roseus cells</title><author>Vogel, H.J ; Lundberg, P ; Bagh, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-f0807b4c187d8b0d92e1d1a8f0ae971a5945a6b58d64039ce2eaaf2898acf7b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Agronomy. 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Psychology</topic><topic>glutamate-ammonia ligase</topic><topic>In vitro culture</topic><topic>metabolism</topic><topic>metabolites</topic><topic>Nuclear magnetic resonance</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>nutrient reserves</topic><topic>nutrient uptake</topic><topic>phosphates</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Tissue cultures, protoplasts</topic><topic>Vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vogel, H.J</creatorcontrib><creatorcontrib>Lundberg, P</creatorcontrib><creatorcontrib>Bagh, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>In vitro cellular & developmental biology. 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In this review we show how phosphorus-31 NMR is used to study intracellular pH, phosphate uptake and storage, and energy metabolism in suspension cells of Catharanthus roseus. In addition, multinuclear NMR studies of the uptake of ammonium and the gradients of K+over the membrane are discussed as well. The use of two-dimensional NMR for the study of whole cell extracts is also described. Finally, we show how nitrogen-14 and nitrogen-15 NMR are used to obtain information about the assimilation of inorganic sources in developing carrot somatic embryos. These NMR studies provide a unique insight into the metabolism of tissue-cultured plant cells.</abstract><cop>Wallingford</cop><pub>Society for In Vitro Biology</pub><doi>10.1007/s11627-999-0024-y</doi><tpages>8</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 1999-03, Vol.35 (2), p.144-151 |
| issn | 1054-5476 1475-2689 |
| language | eng |
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| subjects | Agronomy. Soil science and plant productions Amino acid metabolism ammonium compounds Biological and medical sciences Biotechnology Catharanthus roseus Cell extracts cell suspension culture Cellular metabolism Cultured cells Cytoplasm Economic plant physiology Energy metabolism Fundamental and applied biological sciences. Psychology glutamate-ammonia ligase In vitro culture metabolism metabolites Nuclear magnetic resonance nuclear magnetic resonance spectroscopy nutrient reserves nutrient uptake phosphates Plant cells Plant physiology and development Plants Tissue cultures, protoplasts Vacuoles |
| title | Noninvasive NMR studies of metabolism in cultured Catharanthus roseus cells |
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