Degradation of polyethylene by Penicillium simplicissimum isolated from local dumpsite of Shivamogga district
Penicillium simplicissimum was isolated from a local dumpsite of Shivamogga district for use in the biodegradation of polyethylene. Degradation was carried out using autoclaved, UV-treated and surface-sterilized polyethylene. Degradation was monitored by observing weight loss and changes in physical...
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| Veröffentlicht in: | Environment, development and sustainability development and sustainability, 2015-08, Vol.17 (4), p.731-745 |
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| creator | Sowmya, H. V. Ramalingappa Krishnappa, M. Thippeswamy, B. |
| description | Penicillium simplicissimum
was isolated from a local dumpsite of Shivamogga district for use in the biodegradation of polyethylene. Degradation was carried out using autoclaved, UV-treated and surface-sterilized polyethylene. Degradation was monitored by observing weight loss and changes in physical structure by scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
P. simplicissimum
was able to degrade treated polyethylene (38 %) more efficiently than autoclaved (16 %) and surface-sterilized polyethylene (7.7 %). Enzymes responsible for polyethylene degradation were screened from
P. simplicissimum
. Enzymes were identified as laccase and manganese peroxidase. These enzymes were produced in large amount, enzyme activity was calculated using spectrophotometric method, and crude extraction of enzymes was carried out. Molecular weight of laccase was determined as 66 kDa and that of manganese peroxidase was 60 kDa. Capacity of crude enzymes to degrade polyethylene was also determined. By observing these results, we can conclude that
P. simplicissimum
may act as solution for the problem caused by polyethylene in nature. |
| doi_str_mv | 10.1007/s10668-014-9571-4 |
| format | Article |
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was isolated from a local dumpsite of Shivamogga district for use in the biodegradation of polyethylene. Degradation was carried out using autoclaved, UV-treated and surface-sterilized polyethylene. Degradation was monitored by observing weight loss and changes in physical structure by scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
P. simplicissimum
was able to degrade treated polyethylene (38 %) more efficiently than autoclaved (16 %) and surface-sterilized polyethylene (7.7 %). Enzymes responsible for polyethylene degradation were screened from
P. simplicissimum
. Enzymes were identified as laccase and manganese peroxidase. These enzymes were produced in large amount, enzyme activity was calculated using spectrophotometric method, and crude extraction of enzymes was carried out. Molecular weight of laccase was determined as 66 kDa and that of manganese peroxidase was 60 kDa. Capacity of crude enzymes to degrade polyethylene was also determined. By observing these results, we can conclude that
P. simplicissimum
may act as solution for the problem caused by polyethylene in nature.</description><identifier>ISSN: 1387-585X</identifier><identifier>EISSN: 1573-2975</identifier><identifier>DOI: 10.1007/s10668-014-9571-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Autoclaving ; Biodegradation ; Bioremediation ; Chemicals ; Degradation ; Earth and Environmental Science ; Ecology ; Economic Geology ; Economic Growth ; Environment ; Environmental degradation ; Environmental Economics ; Environmental Management ; Environmental science ; Enzymatic activity ; Enzymes ; Fourier transforms ; Fungi ; Infrared spectroscopy ; Laboratories ; Laccase ; Magnetic resonance spectroscopy ; Manganese ; Manganese peroxidase ; Molecular weight ; NMR ; Nuclear magnetic resonance ; Penicillium simplicissimum ; Peroxidase ; Plastics ; Polyethylene ; Polyethylenes ; Proteins ; Scanning electron microscopy ; Sodium ; Spectrophotometry ; Spectrum analysis ; Studies ; Sustainability ; Sustainable Development</subject><ispartof>Environment, development and sustainability, 2015-08, Vol.17 (4), p.731-745</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-5ee199737d5007e1f3b50b24a88abcc2b32a87b7ef33e88cb1877488cd612ebe3</citedby><cites>FETCH-LOGICAL-c528t-5ee199737d5007e1f3b50b24a88abcc2b32a87b7ef33e88cb1877488cd612ebe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10668-014-9571-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10668-014-9571-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27902,27903,41466,42535,51296</link.rule.ids></links><search><creatorcontrib>Sowmya, H. V.</creatorcontrib><creatorcontrib>Ramalingappa</creatorcontrib><creatorcontrib>Krishnappa, M.</creatorcontrib><creatorcontrib>Thippeswamy, B.</creatorcontrib><title>Degradation of polyethylene by Penicillium simplicissimum isolated from local dumpsite of Shivamogga district</title><title>Environment, development and sustainability</title><addtitle>Environ Dev Sustain</addtitle><description>Penicillium simplicissimum
was isolated from a local dumpsite of Shivamogga district for use in the biodegradation of polyethylene. Degradation was carried out using autoclaved, UV-treated and surface-sterilized polyethylene. Degradation was monitored by observing weight loss and changes in physical structure by scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
P. simplicissimum
was able to degrade treated polyethylene (38 %) more efficiently than autoclaved (16 %) and surface-sterilized polyethylene (7.7 %). Enzymes responsible for polyethylene degradation were screened from
P. simplicissimum
. Enzymes were identified as laccase and manganese peroxidase. These enzymes were produced in large amount, enzyme activity was calculated using spectrophotometric method, and crude extraction of enzymes was carried out. Molecular weight of laccase was determined as 66 kDa and that of manganese peroxidase was 60 kDa. Capacity of crude enzymes to degrade polyethylene was also determined. By observing these results, we can conclude that
P. simplicissimum
may act as solution for the problem caused by polyethylene in nature.</description><subject>Autoclaving</subject><subject>Biodegradation</subject><subject>Bioremediation</subject><subject>Chemicals</subject><subject>Degradation</subject><subject>Earth and Environmental Science</subject><subject>Ecology</subject><subject>Economic Geology</subject><subject>Economic Growth</subject><subject>Environment</subject><subject>Environmental degradation</subject><subject>Environmental Economics</subject><subject>Environmental Management</subject><subject>Environmental science</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Fourier transforms</subject><subject>Fungi</subject><subject>Infrared spectroscopy</subject><subject>Laboratories</subject><subject>Laccase</subject><subject>Magnetic resonance spectroscopy</subject><subject>Manganese</subject><subject>Manganese peroxidase</subject><subject>Molecular weight</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Penicillium simplicissimum</subject><subject>Peroxidase</subject><subject>Plastics</subject><subject>Polyethylene</subject><subject>Polyethylenes</subject><subject>Proteins</subject><subject>Scanning electron microscopy</subject><subject>Sodium</subject><subject>Spectrophotometry</subject><subject>Spectrum analysis</subject><subject>Studies</subject><subject>Sustainability</subject><subject>Sustainable Development</subject><issn>1387-585X</issn><issn>1573-2975</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqNkU9r3DAQxU1poNtNP0BvglxycaORLEs-lvRfIJBAEshNyPJ4V4tsuZId2G8fLZtDCARyGOYN_N6D4RXFd6A_gFJ5kYDWtSopVGUjJJTVp2IFQvKSNVJ8zporWQolHr8UX1PaUcpow-pVMfzCTTSdmV0YSejJFPwe5-3e44ik3ZNbHJ113rtlIMkNk89XyiKfLgVvZuxIH8NAfLDGk24ZpuRmPETdbd2TGcJmY0jn0hydnU-Lk974hN9e9rp4-PP7_vJfeX3z9-ry53VpBVNzKRChaSSXnci_IfS8FbRllVHKtNayljOjZCux5xyVsi0oKassuhoYtsjXxfkxd4rh_4Jp1oNLFr03I4YlaZCcUpWHfQClDSgKjH4ErXgFTXVIPXuD7sISx_yzhrrhktEMZwqOlI0hpYi9nqIbTNxroPpQqz7WqnOt-lCrrrKHHT0ps-MG46vkd03Pf0Gl7g</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Sowmya, H. 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V. ; Ramalingappa ; Krishnappa, M. ; Thippeswamy, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-5ee199737d5007e1f3b50b24a88abcc2b32a87b7ef33e88cb1877488cd612ebe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Autoclaving</topic><topic>Biodegradation</topic><topic>Bioremediation</topic><topic>Chemicals</topic><topic>Degradation</topic><topic>Earth and Environmental Science</topic><topic>Ecology</topic><topic>Economic Geology</topic><topic>Economic Growth</topic><topic>Environment</topic><topic>Environmental degradation</topic><topic>Environmental Economics</topic><topic>Environmental Management</topic><topic>Environmental science</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Fourier transforms</topic><topic>Fungi</topic><topic>Infrared spectroscopy</topic><topic>Laboratories</topic><topic>Laccase</topic><topic>Magnetic resonance spectroscopy</topic><topic>Manganese</topic><topic>Manganese peroxidase</topic><topic>Molecular weight</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Penicillium simplicissimum</topic><topic>Peroxidase</topic><topic>Plastics</topic><topic>Polyethylene</topic><topic>Polyethylenes</topic><topic>Proteins</topic><topic>Scanning electron microscopy</topic><topic>Sodium</topic><topic>Spectrophotometry</topic><topic>Spectrum analysis</topic><topic>Studies</topic><topic>Sustainability</topic><topic>Sustainable Development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sowmya, H. V.</creatorcontrib><creatorcontrib>Ramalingappa</creatorcontrib><creatorcontrib>Krishnappa, M.</creatorcontrib><creatorcontrib>Thippeswamy, B.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>International Bibliography of the Social Sciences (IBSS)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>International Bibliography of the Social Sciences</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>International Bibliography of the Social Sciences</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>Civil Engineering Abstracts</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Engineering Collection</collection><collection>ABI/INFORM Global</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Research Database</collection><jtitle>Environment, development and sustainability</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sowmya, H. V.</au><au>Ramalingappa</au><au>Krishnappa, M.</au><au>Thippeswamy, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degradation of polyethylene by Penicillium simplicissimum isolated from local dumpsite of Shivamogga district</atitle><jtitle>Environment, development and sustainability</jtitle><stitle>Environ Dev Sustain</stitle><date>2015-08-01</date><risdate>2015</risdate><volume>17</volume><issue>4</issue><spage>731</spage><epage>745</epage><pages>731-745</pages><issn>1387-585X</issn><eissn>1573-2975</eissn><abstract>Penicillium simplicissimum
was isolated from a local dumpsite of Shivamogga district for use in the biodegradation of polyethylene. Degradation was carried out using autoclaved, UV-treated and surface-sterilized polyethylene. Degradation was monitored by observing weight loss and changes in physical structure by scanning electron microscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.
P. simplicissimum
was able to degrade treated polyethylene (38 %) more efficiently than autoclaved (16 %) and surface-sterilized polyethylene (7.7 %). Enzymes responsible for polyethylene degradation were screened from
P. simplicissimum
. Enzymes were identified as laccase and manganese peroxidase. These enzymes were produced in large amount, enzyme activity was calculated using spectrophotometric method, and crude extraction of enzymes was carried out. Molecular weight of laccase was determined as 66 kDa and that of manganese peroxidase was 60 kDa. Capacity of crude enzymes to degrade polyethylene was also determined. By observing these results, we can conclude that
P. simplicissimum
may act as solution for the problem caused by polyethylene in nature.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10668-014-9571-4</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Environment, development and sustainability, 2015-08, Vol.17 (4), p.731-745 |
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| subjects | Autoclaving Biodegradation Bioremediation Chemicals Degradation Earth and Environmental Science Ecology Economic Geology Economic Growth Environment Environmental degradation Environmental Economics Environmental Management Environmental science Enzymatic activity Enzymes Fourier transforms Fungi Infrared spectroscopy Laboratories Laccase Magnetic resonance spectroscopy Manganese Manganese peroxidase Molecular weight NMR Nuclear magnetic resonance Penicillium simplicissimum Peroxidase Plastics Polyethylene Polyethylenes Proteins Scanning electron microscopy Sodium Spectrophotometry Spectrum analysis Studies Sustainability Sustainable Development |
| title | Degradation of polyethylene by Penicillium simplicissimum isolated from local dumpsite of Shivamogga district |
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