Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice

Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each puri...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2015-12, Vol.120 (6), p.715-719
Hauptverfasser:	Yoshimoto, Mai, Otsuki, Takahiro, Itagaki, Kohei, Kato, Tatsuya, Kohsaka, Tetsuya, Matsumoto, Yumino, Ike, Kazunori, Park, Enoch Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigen Antigens, Protozoan - genetics Antigens, Protozoan - immunology Antigens, Protozoan - isolation & purification BmNPV bacmid Bombyx Coccidiosis - immunology Coccidiosis - prevention & control Female Hemolymph - chemistry Immunization Larva Mice Mice, Inbred BALB C Neospora - chemistry Neospora - genetics Neospora - immunology Neospora caninum Protozoan Vaccines - chemistry Protozoan Vaccines - immunology Recombinant Proteins - isolation & purification Silkworm Subunit vaccine Th1 Cells - immunology Th2 Cells - immunology Vaccines, Subunit - chemistry Vaccines, Subunit - immunology
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