Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice
Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each puri...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2015-12, Vol.120 (6), p.715-719 |
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| container_title | Journal of bioscience and bioengineering |
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| creator | Yoshimoto, Mai Otsuki, Takahiro Itagaki, Kohei Kato, Tatsuya Kohsaka, Tetsuya Matsumoto, Yumino Ike, Kazunori Park, Enoch Y. |
| description | Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each purified recombinant antigen was immunized with Freund's incomplete adjuvant (FIA) to mice, recombinant NcSAG1 induced a Th2 immune response in immunized mice and produced a SAG1-specific antibody. In the experiment where NcSAG1-immunized mice were challenged with N. caninum, the cerebral N. caninum burden was significantly reduced compared with that of either the FIA- or PBS-immunized mice. Recombinant NcSRS2 or NcMIC3 induced both Th1 and Th2 immune responses, but NcMIC3-immunization did not induce significant production of NcMIC3-specific antibodies. These results suggest that the silkworm can produce recombinant antigens of N. caninum, which can be used as a recombinant vaccine against N. caninum. |
| doi_str_mv | 10.1016/j.jbiosc.2015.04.002 |
| format | Article |
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From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each purified recombinant antigen was immunized with Freund's incomplete adjuvant (FIA) to mice, recombinant NcSAG1 induced a Th2 immune response in immunized mice and produced a SAG1-specific antibody. In the experiment where NcSAG1-immunized mice were challenged with N. caninum, the cerebral N. caninum burden was significantly reduced compared with that of either the FIA- or PBS-immunized mice. Recombinant NcSRS2 or NcMIC3 induced both Th1 and Th2 immune responses, but NcMIC3-immunization did not induce significant production of NcMIC3-specific antibodies. These results suggest that the silkworm can produce recombinant antigens of N. caninum, which can be used as a recombinant vaccine against N. caninum.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2015.04.002</identifier><identifier>PMID: 25935502</identifier><language>eng</language><publisher>Japan: Elsevier B.V</publisher><subject>Animals ; Antigen ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Antigens, Protozoan - isolation & purification ; BmNPV bacmid ; Bombyx ; Coccidiosis - immunology ; Coccidiosis - prevention & control ; Female ; Hemolymph - chemistry ; Immunization ; Larva ; Mice ; Mice, Inbred BALB C ; Neospora - chemistry ; Neospora - genetics ; Neospora - immunology ; Neospora caninum ; Protozoan Vaccines - chemistry ; Protozoan Vaccines - immunology ; Recombinant Proteins - isolation & purification ; Silkworm ; Subunit vaccine ; Th1 Cells - immunology ; Th2 Cells - immunology ; Vaccines, Subunit - chemistry ; Vaccines, Subunit - immunology</subject><ispartof>Journal of bioscience and bioengineering, 2015-12, Vol.120 (6), p.715-719</ispartof><rights>2015 The Society for Biotechnology, Japan</rights><rights>Copyright © 2015 The Society for Biotechnology, Japan. 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All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-d4310c4166e15d29a9e6a84de4e873aca8c131112ea97e44a99dd89131cbe3533</citedby><cites>FETCH-LOGICAL-c525t-d4310c4166e15d29a9e6a84de4e873aca8c131112ea97e44a99dd89131cbe3533</cites><orcidid>0000-0002-7840-1424</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiosc.2015.04.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25935502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshimoto, Mai</creatorcontrib><creatorcontrib>Otsuki, Takahiro</creatorcontrib><creatorcontrib>Itagaki, Kohei</creatorcontrib><creatorcontrib>Kato, Tatsuya</creatorcontrib><creatorcontrib>Kohsaka, Tetsuya</creatorcontrib><creatorcontrib>Matsumoto, Yumino</creatorcontrib><creatorcontrib>Ike, Kazunori</creatorcontrib><creatorcontrib>Park, Enoch Y.</creatorcontrib><title>Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each purified recombinant antigen was immunized with Freund's incomplete adjuvant (FIA) to mice, recombinant NcSAG1 induced a Th2 immune response in immunized mice and produced a SAG1-specific antibody. In the experiment where NcSAG1-immunized mice were challenged with N. caninum, the cerebral N. caninum burden was significantly reduced compared with that of either the FIA- or PBS-immunized mice. Recombinant NcSRS2 or NcMIC3 induced both Th1 and Th2 immune responses, but NcMIC3-immunization did not induce significant production of NcMIC3-specific antibodies. These results suggest that the silkworm can produce recombinant antigens of N. caninum, which can be used as a recombinant vaccine against N. caninum.</description><subject>Animals</subject><subject>Antigen</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Antigens, Protozoan - isolation & purification</subject><subject>BmNPV bacmid</subject><subject>Bombyx</subject><subject>Coccidiosis - immunology</subject><subject>Coccidiosis - prevention & control</subject><subject>Female</subject><subject>Hemolymph - chemistry</subject><subject>Immunization</subject><subject>Larva</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neospora - chemistry</subject><subject>Neospora - genetics</subject><subject>Neospora - immunology</subject><subject>Neospora caninum</subject><subject>Protozoan Vaccines - chemistry</subject><subject>Protozoan Vaccines - immunology</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Silkworm</subject><subject>Subunit vaccine</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - immunology</subject><subject>Vaccines, Subunit - chemistry</subject><subject>Vaccines, Subunit - immunology</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu2zAQJIIWebV_UAQ89iKVS1KWeAkQBEkaIEguyZmgyVVLVyIdUnLRD-h_5Fv6ZaVhu8eedrGcmV3OEPIJWA0MFl9W9WrpY7Y1Z9DUTNaM8SNyCkK2lZQc3m37TlXQcnFCznJeMQYta-GYnPBGiaZh_JT8vtmYYTaTj4HGnia0cVz6YMJEHzHmdUyGWhN8mEdahv4bhkzXc_K9R0f7FEea_fDjZ0wjHUzaGKR9THT6jnSd4oT2oPxY_3k7CPnQ7x98oKO3-IG8782Q8eO-npOX25vn66_Vw9Pd_fXVQ2Ub3kyVkwKYlbBYIDSOK6NwYTrpUGLXCmNNZ0EAAEejWpTSKOVcp8rMLlE0QpyTzzvdctvrjHnSo88Wh8EEjHPW0IriIleiLVC5g9oUc07Y63Xyo0m_NDC9DUCv9C4AvQ1AM6kLtdAu9hvm5YjuH-ngeAFc7gBY_rnxmHS2HoNF54v5k3bR_3_DX2Prm9E</recordid><startdate>201512</startdate><enddate>201512</enddate><creator>Yoshimoto, Mai</creator><creator>Otsuki, Takahiro</creator><creator>Itagaki, Kohei</creator><creator>Kato, Tatsuya</creator><creator>Kohsaka, Tetsuya</creator><creator>Matsumoto, Yumino</creator><creator>Ike, Kazunori</creator><creator>Park, Enoch Y.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7840-1424</orcidid></search><sort><creationdate>201512</creationdate><title>Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice</title><author>Yoshimoto, Mai ; Otsuki, Takahiro ; Itagaki, Kohei ; Kato, Tatsuya ; Kohsaka, Tetsuya ; Matsumoto, Yumino ; Ike, Kazunori ; Park, Enoch Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-d4310c4166e15d29a9e6a84de4e873aca8c131112ea97e44a99dd89131cbe3533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antigen</topic><topic>Antigens, Protozoan - genetics</topic><topic>Antigens, Protozoan - immunology</topic><topic>Antigens, Protozoan - isolation & purification</topic><topic>BmNPV bacmid</topic><topic>Bombyx</topic><topic>Coccidiosis - immunology</topic><topic>Coccidiosis - prevention & control</topic><topic>Female</topic><topic>Hemolymph - chemistry</topic><topic>Immunization</topic><topic>Larva</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neospora - chemistry</topic><topic>Neospora - genetics</topic><topic>Neospora - immunology</topic><topic>Neospora caninum</topic><topic>Protozoan Vaccines - chemistry</topic><topic>Protozoan Vaccines - immunology</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Silkworm</topic><topic>Subunit vaccine</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - immunology</topic><topic>Vaccines, Subunit - chemistry</topic><topic>Vaccines, Subunit - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshimoto, Mai</creatorcontrib><creatorcontrib>Otsuki, Takahiro</creatorcontrib><creatorcontrib>Itagaki, Kohei</creatorcontrib><creatorcontrib>Kato, Tatsuya</creatorcontrib><creatorcontrib>Kohsaka, Tetsuya</creatorcontrib><creatorcontrib>Matsumoto, Yumino</creatorcontrib><creatorcontrib>Ike, Kazunori</creatorcontrib><creatorcontrib>Park, Enoch Y.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshimoto, Mai</au><au>Otsuki, Takahiro</au><au>Itagaki, Kohei</au><au>Kato, Tatsuya</au><au>Kohsaka, Tetsuya</au><au>Matsumoto, Yumino</au><au>Ike, Kazunori</au><au>Park, Enoch Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2015-12</date><risdate>2015</risdate><volume>120</volume><issue>6</issue><spage>715</spage><epage>719</epage><pages>715-719</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Three antigens (NcSAG1, NcSRS2 and NcMIC3) from Neospora caninum were expressed using the BmNPV bacmid system in silkworm larvae and purified from the hemolymph. From 20 silkworm larvae, 1.5, 1.2 and 1.4 mg of purified recombinant NcSAG1, NcSRS2 and NcMIC3 were obtained, respectively. When each purified recombinant antigen was immunized with Freund's incomplete adjuvant (FIA) to mice, recombinant NcSAG1 induced a Th2 immune response in immunized mice and produced a SAG1-specific antibody. In the experiment where NcSAG1-immunized mice were challenged with N. caninum, the cerebral N. caninum burden was significantly reduced compared with that of either the FIA- or PBS-immunized mice. Recombinant NcSRS2 or NcMIC3 induced both Th1 and Th2 immune responses, but NcMIC3-immunization did not induce significant production of NcMIC3-specific antibodies. These results suggest that the silkworm can produce recombinant antigens of N. caninum, which can be used as a recombinant vaccine against N. caninum.</abstract><cop>Japan</cop><pub>Elsevier B.V</pub><pmid>25935502</pmid><doi>10.1016/j.jbiosc.2015.04.002</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-7840-1424</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of bioscience and bioengineering, 2015-12, Vol.120 (6), p.715-719 |
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| subjects | Animals Antigen Antigens, Protozoan - genetics Antigens, Protozoan - immunology Antigens, Protozoan - isolation & purification BmNPV bacmid Bombyx Coccidiosis - immunology Coccidiosis - prevention & control Female Hemolymph - chemistry Immunization Larva Mice Mice, Inbred BALB C Neospora - chemistry Neospora - genetics Neospora - immunology Neospora caninum Protozoan Vaccines - chemistry Protozoan Vaccines - immunology Recombinant Proteins - isolation & purification Silkworm Subunit vaccine Th1 Cells - immunology Th2 Cells - immunology Vaccines, Subunit - chemistry Vaccines, Subunit - immunology |
| title | Evaluation of recombinant Neospora caninum antigens purified from silkworm larvae for the protection of N. caninum infection in mice |
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