A Dye-Decolorizing Peroxidase from Vibrio cholerae

The dye-decolorizing peroxidase (DyP) protein from Vibrio cholerae (VcDyP) was expressed in Escherichia coli, and its DyP activity was assayed by monitoring degradation of a typical anthraquinone dye, reactive blue 19 (RB19). Its kinetic activity was obtained by fitting the data to the Michaelis–Men...

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Veröffentlicht in:	Biochemistry (Easton) 2015-11, Vol.54 (43), p.6610-6621
Hauptverfasser:	Uchida, Takeshi, Sasaki, Miho, Tanaka, Yoshikazu, Ishimori, Koichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Catalytic Domain - genetics Coloring Agents - metabolism Crystallography, X-Ray Hydrogen-Ion Concentration Kinetics Models, Molecular Mutagenesis, Site-Directed Peroxidases - chemistry Peroxidases - genetics Peroxidases - metabolism Protein Conformation Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Spectrophotometry Spectrum Analysis, Raman Vibrio cholerae - enzymology Vibrio cholerae - genetics
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