A colorimetric assay for screening microcystin class compounds in aquatic systems
Secondary metabolites produced by water-blooming cyanobacteria in eutrophic waters include some potent hepatotoxins. These compounds also have tumour-promoting properties, attributable to their inhibition and activation of protein phosphatases and kinases respectively. The inhibitory effect of these...
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| Veröffentlicht in: | Chemosphere (Oxford) 1999-02, Vol.38 (5), p.1113-1122 |
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| container_title | Chemosphere (Oxford) |
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| creator | Wong, Bryan S.F. Lam, Paul K.S. Xu, Lihong Zhang, Yongyuan Richardson, Bruce J. |
| description | Secondary metabolites produced by water-blooming cyanobacteria in eutrophic waters include some potent hepatotoxins. These compounds also have tumour-promoting properties, attributable to their inhibition and activation of protein phosphatases and kinases respectively. The inhibitory effect of these toxins on protein phosphatases have been employed in a commonly used radiometric assay, involving the use of a
32p-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow
p-nitrophenol using
p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay. |
| doi_str_mv | 10.1016/S0045-6535(98)00354-3 |
| format | Article |
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32p-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow
p-nitrophenol using
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32p-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow
p-nitrophenol using
p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay.</description><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Environmental pollutants toxicology</subject><subject>Fundamental and applied biological sciences. 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32p-labeled substrate, for the detection and quantitation of these compounds. This paper investigates and describes a colorimetric method in which the activity of protein phosphatase 2A is determined by measuring the rate of colour production from the release of yellow
p-nitrophenol using
p-nitrophenyl phosphate as the substrate. Results of this study suggest that the colorimetric protein phosphatase inhibition assay is a simple, inexpensive tool for screening substances that may have tumour-promoting characteristics in aquatic systems. The detection limit of the colorimetric method is comparable to the radiometric assay.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0045-6535(98)00354-3</doi><tpages>10</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Animal, plant and microbial ecology Applied ecology Biological and medical sciences Ecotoxicology, biological effects of pollution Environmental pollutants toxicology Fundamental and applied biological sciences. Psychology Medical sciences Techniques Toxicology Water |
| title | A colorimetric assay for screening microcystin class compounds in aquatic systems |
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