Combining confocal and single molecule localisation microscopy: A correlative approach to multi-scale tissue imaging

•A method of multi-scale imaging of human heart was developed.•This was achieved by correlatively imaging with confocal and dSTORM.•Correlative imaging reveals nanoscale colocalisation of membrane receptors.•Methods for labelling, imaging, registering and analysing data are described. Many biologica...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2015-10, Vol.88, p.98-108
Hauptverfasser:	Crossman, David J., Hou, Yufeng, Jayasinghe, Izzy, Baddeley, David, Soeller, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Correlative microscopy dSTORM Human heart tissue Humans Membrane Proteins Microscopy, Confocal - methods Microscopy, Fluorescence - methods Molecular Imaging - methods Myocardium - cytology Myocardium - metabolism Ryanodine Receptor Calcium Release Channel Single molecule localisation microscopy Super-resolution
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