In vitro analysis of a mammalian retinal progenitor that gives rise to neurons and glia
In vivo lineage studies have shown that retinal cells arise from multipotential progenitors whose fates are regulated by cell–cell interactions. To understand the mechanism underlying their maintenance and differentiation, we have analyzed the differentiation potential of progenitors derived from em...
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| Veröffentlicht in: | Brain research 1999-06, Vol.831 (1), p.1-10 |
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| creator | Ahmad, Iqbal Dooley, Constance M Thoreson, Wallace B Rogers, Jim A Afiat, Sanaz |
| description | In vivo lineage studies have shown that retinal cells arise from multipotential progenitors whose fates are regulated by cell–cell interactions. To understand the mechanism underlying their maintenance and differentiation, we have analyzed the differentiation potential of progenitors derived from embryonic rat retina in vitro. These progenitors proliferate and remain undifferentiated in vitro in the presence of epidermal growth factor (EGF) and display properties similar to stem cells. In addition to expressing nestin, the neuroectodermal stem cell marker, retinal progenitors are multipotential. Upon withdrawal of EGF and addition of serum, the progenitors downregulate the expression of nestin and express cell-type specific markers corresponding to neurons and glia. In addition to expressing cell-type specific markers, retinal progenitors and their progeny could be distinguished on the basis of their distinct voltage gated current profile. A proportion of progenitors is lineage restricted and the fate of these cells can be influenced by the microenvironment, suggesting that stage-specific interactions mediated by the local environment influence the progression of progenitors towards acquisition of differentiated phenotypes. |
| doi_str_mv | 10.1016/S0006-8993(99)01376-1 |
| format | Article |
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To understand the mechanism underlying their maintenance and differentiation, we have analyzed the differentiation potential of progenitors derived from embryonic rat retina in vitro. These progenitors proliferate and remain undifferentiated in vitro in the presence of epidermal growth factor (EGF) and display properties similar to stem cells. In addition to expressing nestin, the neuroectodermal stem cell marker, retinal progenitors are multipotential. Upon withdrawal of EGF and addition of serum, the progenitors downregulate the expression of nestin and express cell-type specific markers corresponding to neurons and glia. In addition to expressing cell-type specific markers, retinal progenitors and their progeny could be distinguished on the basis of their distinct voltage gated current profile. 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Psychology ; Neuroglia - cytology ; Neuroglia - drug effects ; Neurons - cytology ; Neurons - drug effects ; Phenotype ; Progenitor ; Rats ; Rats, Sprague-Dawley ; Retina ; Retina - cytology ; Retina - drug effects ; Retina - embryology ; Rod photoreceptor ; Stem cell ; Stem Cells - drug effects ; Stem Cells - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1999-06, Vol.831 (1), p.1-10</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-18780b77d534db38fa49d3dee310252c8cb8287f034f5379c9df27b64c9ff7eb3</citedby><cites>FETCH-LOGICAL-c390t-18780b77d534db38fa49d3dee310252c8cb8287f034f5379c9df27b64c9ff7eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-8993(99)01376-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1870717$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10411978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahmad, Iqbal</creatorcontrib><creatorcontrib>Dooley, Constance M</creatorcontrib><creatorcontrib>Thoreson, Wallace B</creatorcontrib><creatorcontrib>Rogers, Jim A</creatorcontrib><creatorcontrib>Afiat, Sanaz</creatorcontrib><title>In vitro analysis of a mammalian retinal progenitor that gives rise to neurons and glia</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>In vivo lineage studies have shown that retinal cells arise from multipotential progenitors whose fates are regulated by cell–cell interactions. To understand the mechanism underlying their maintenance and differentiation, we have analyzed the differentiation potential of progenitors derived from embryonic rat retina in vitro. These progenitors proliferate and remain undifferentiated in vitro in the presence of epidermal growth factor (EGF) and display properties similar to stem cells. In addition to expressing nestin, the neuroectodermal stem cell marker, retinal progenitors are multipotential. Upon withdrawal of EGF and addition of serum, the progenitors downregulate the expression of nestin and express cell-type specific markers corresponding to neurons and glia. In addition to expressing cell-type specific markers, retinal progenitors and their progeny could be distinguished on the basis of their distinct voltage gated current profile. A proportion of progenitors is lineage restricted and the fate of these cells can be influenced by the microenvironment, suggesting that stage-specific interactions mediated by the local environment influence the progression of progenitors towards acquisition of differentiated phenotypes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Lineage</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Differentiation</subject><subject>Electrophysiology</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. 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Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - drug effects</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Phenotype</topic><topic>Progenitor</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Retina</topic><topic>Retina - cytology</topic><topic>Retina - drug effects</topic><topic>Retina - embryology</topic><topic>Rod photoreceptor</topic><topic>Stem cell</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahmad, Iqbal</creatorcontrib><creatorcontrib>Dooley, Constance M</creatorcontrib><creatorcontrib>Thoreson, Wallace B</creatorcontrib><creatorcontrib>Rogers, Jim A</creatorcontrib><creatorcontrib>Afiat, Sanaz</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahmad, Iqbal</au><au>Dooley, Constance M</au><au>Thoreson, Wallace B</au><au>Rogers, Jim A</au><au>Afiat, Sanaz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro analysis of a mammalian retinal progenitor that gives rise to neurons and glia</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1999-06-12</date><risdate>1999</risdate><volume>831</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>In vivo lineage studies have shown that retinal cells arise from multipotential progenitors whose fates are regulated by cell–cell interactions. To understand the mechanism underlying their maintenance and differentiation, we have analyzed the differentiation potential of progenitors derived from embryonic rat retina in vitro. These progenitors proliferate and remain undifferentiated in vitro in the presence of epidermal growth factor (EGF) and display properties similar to stem cells. In addition to expressing nestin, the neuroectodermal stem cell marker, retinal progenitors are multipotential. Upon withdrawal of EGF and addition of serum, the progenitors downregulate the expression of nestin and express cell-type specific markers corresponding to neurons and glia. In addition to expressing cell-type specific markers, retinal progenitors and their progeny could be distinguished on the basis of their distinct voltage gated current profile. 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| subjects | Animals Biological and medical sciences Cell Differentiation - drug effects Cell Differentiation - physiology Cell Lineage Cells, Cultured Coculture Techniques Differentiation Electrophysiology Epidermal Growth Factor - pharmacology Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Neuroglia - cytology Neuroglia - drug effects Neurons - cytology Neurons - drug effects Phenotype Progenitor Rats Rats, Sprague-Dawley Retina Retina - cytology Retina - drug effects Retina - embryology Rod photoreceptor Stem cell Stem Cells - drug effects Stem Cells - physiology Vertebrates: nervous system and sense organs |
| title | In vitro analysis of a mammalian retinal progenitor that gives rise to neurons and glia |
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