The assembly and migration of SeqA–Gfp fusion in living cells of Escherichia coli

SeqA protein, which binds to hemi‐methylated GATC sequences of DNA, is localized to discrete fluorescent foci in wild‐type Escherichia coli cells. In this work, we observed cellular localization of the SeqA–Gfp fusion in living cells. SeqA–Gfp was localized to a discrete focus or foci in wild‐type a...

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Veröffentlicht in:	Molecular microbiology 1999-03, Vol.31 (6), p.1775-1782
Hauptverfasser:	Onogi, Toshinari, Niki, Hironori, Yamazoe, Mitsuyoshi, Hiraga, Sota
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Outer Membrane Proteins Bacterial Proteins - metabolism Cell Nucleus - physiology Chromosomal Proteins, Non-Histone DNA Replication - physiology DNA-Binding Proteins Electrophoresis, Polyacrylamide Gel Escherichia coli Escherichia coli - cytology Escherichia coli - physiology Escherichia coli Proteins Flow Cytometry Green Fluorescent Proteins Luminescent Proteins - metabolism Models, Genetic Mutagenesis Plasmids Precipitin Tests Recombinant Fusion Proteins - metabolism Time Factors Transcription Factors
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