Semiautomated metabolic staining assay for Bacillus cereus emetic toxin
This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, o...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1999-04, Vol.65 (4), p.1811-1812 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Finlay, W.J.J Logan, N.A Sutherland, A.D |
| description | This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic. |
| doi_str_mv | 10.1128/aem.65.4.1811-1812.1999 |
| format | Article |
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Psychology</subject><subject>Humans</subject><subject>Metabolism</subject><subject>Microbiology</subject><subject>Tests</subject><subject>Tetrazolium Salts - metabolism</subject><subject>Thiazoles - metabolism</subject><subject>Tumor Cells, Cultured</subject><subject>Vacuoles</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkk1v1DAQhi0EokvhL9CAELcsHsfxh9RLqUpBKuLQcrYmjrPrKomLnQD99zjKihYuM4d53hmP3yHkBOgWgKkP6IatqLd8CwqgzIFtQWv9hGyAalXWVSWekg2lWpeMcXpEXqR0SynlVKjn5Ago0IopvSGX127wOE9hwMm1xeAmbELvbZEm9KMfdwWmhPdFF2LxEa3v-zkV1kWXk8t0Jqfw248vybMO--ReHfIxufl0cXP-ubz6dvnl_OyqtDVXU6mFa4HKthGaMw2WMtch78C2TVNzySg2tZaCOU2Vo4o7kJK1UkhtZSWa6picrm3v5mZwrXXjFLE3d9EPGO9NQG_-rYx-b3bhp9HAap3l7w_yGH7MLk1m8Mm6vsfRhTkZkExxKSCDb_8Db8Mcx7yZYbTWNYBSGZIrZGNIKbru7zuAmsUmc3bx1YjacLPYtARmFpuy8vXjNR7pVl8y8O4AYLLYdxFH69MDJ_P_wIK9WbG93-1_-egMpsHk43iYmpmTlekwGNzF3Ob7NaNQ0TxIMZDVHxi4sGc</recordid><startdate>19990401</startdate><enddate>19990401</enddate><creator>Finlay, W.J.J</creator><creator>Logan, N.A</creator><creator>Sutherland, A.D</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7U7</scope><scope>5PM</scope></search><sort><creationdate>19990401</creationdate><title>Semiautomated metabolic staining assay for Bacillus cereus emetic toxin</title><author>Finlay, W.J.J ; Logan, N.A ; Sutherland, A.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-96ed107db694291c02efa4f1cdbb54720ab59762e908e084e1772d7679c736b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacillus cereus</topic><topic>Bacillus cereus - isolation & purification</topic><topic>Bacillus cereus - metabolism</topic><topic>Bacteria</topic><topic>Bacterial Toxins - biosynthesis</topic><topic>Bacterial Toxins - toxicity</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Coloring Agents - metabolism</topic><topic>Disease</topic><topic>Emetics</topic><topic>food contamination</topic><topic>Food Microbiology</topic><topic>Foodborne Diseases - microbiology</topic><topic>Fundamental and applied biological sciences. 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| ispartof | Applied and Environmental Microbiology, 1999-04, Vol.65 (4), p.1811-1812 |
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| language | eng |
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| source | MEDLINE; American Society for Microbiology Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacillus cereus Bacillus cereus - isolation & purification Bacillus cereus - metabolism Bacteria Bacterial Toxins - biosynthesis Bacterial Toxins - toxicity Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Coloring Agents - metabolism Disease Emetics food contamination Food Microbiology Foodborne Diseases - microbiology Fundamental and applied biological sciences. Psychology Humans Metabolism Microbiology Tests Tetrazolium Salts - metabolism Thiazoles - metabolism Tumor Cells, Cultured Vacuoles |
| title | Semiautomated metabolic staining assay for Bacillus cereus emetic toxin |
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