Menstrual blood contains immune cells with inflammatory and anti-inflammatory properties
Aim Successful pregnancy requires balanced regulation of immune cells at the feto‐maternal interface. Systemic monitoring of the immune system cannot precisely outline local immune status in the uterus. In this survey, endometrial immune milieu was investigated using a non‐invasive method of analysi...
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| Veröffentlicht in: | The journal of obstetrics and gynaecology research 2015-11, Vol.41 (11), p.1803-1812 |
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| container_title | The journal of obstetrics and gynaecology research |
| container_volume | 41 |
| creator | Hosseini, Samira Shokri, Fazel Tokhmechy, Reihaneh Savadi-Shiraz, Elham Jeddi-Tehrani, Mahmood Rahbari, Marjaneh Zarnani, Amir-Hassan |
| description | Aim
Successful pregnancy requires balanced regulation of immune cells at the feto‐maternal interface. Systemic monitoring of the immune system cannot precisely outline local immune status in the uterus. In this survey, endometrial immune milieu was investigated using a non‐invasive method of analysis of menstrual blood (MB). The results were compared with peripheral blood (PB).
Method
PB and MB of healthy fertile women (n = 15) were collected simultaneously on the second day of their menstrual cycle. T and natural killer T cell subpopulations were immunophenotyped by flow cytometry.
Results
Among examined cell populations, the frequency of CD4 + Foxp3+, CD4 + Foxp3 + CD25‐, CD4 + Foxp3 + CD25+ and IL17+ T cells (P = 0.022, 0.028, 0.017 and 0.005, respectively) and TCRαβ+, CD45RO+, CD16‐, IFNγ + and IL17+ NKT (CD56 + CD3+) cells (P = 0.010, 0.037, 0.038, 0.015 and 0.021, respectively) were significantly higher in MB compared with PB. Conversely, PB contained a higher percentage of CD16+ T cells (P = 0.025) in comparison with MB.
Conclusion
MB contains cells of an inflammatory and anti‐inflammatory nature, implying the existence of finely tuned cell homeostasis during menstruation. Our results imply that MB could be viewed as an easy‐to access specimen for monitoring endometrial immune cells, especially those that have preferential endometrial localization. |
| doi_str_mv | 10.1111/jog.12801 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1728259474</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1728259474</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4571-2714079687ef35331fcfae347577093e2479cc787950d47a7ed2a04fc4ee4dea3</originalsourceid><addsrcrecordid>eNp1kE1PwzAMhiMEgjE48AdQj3DoljRp3R7RgAEbTEJDcIuy1oVAP0bSauzfk7ENiQOWLEfW4zf2S8gJoz3mov9ev_ZYEFO2QzpMCPAphNGue3PB_JhCdEAOrX2nlEHC4n1yEEQcKARxh7zcY2Ub06rCmxV1nXlpXTVKV9bTZdlW6KVYFNZb6ObN01VeqLJUTW2Wnqoyl432_3Tnpp6jaTTaI7KXq8Li8aZ2ydP11XRw448nw9vBxdhPRQjMD4AJCkkUA-Y85Jzlaa6QCwgBaMIxEJCkKcSQhDQToACzQFGRpwJRZKh4l5ytdd3Xny3aRpbarpZWFdatlcydGYSJAOHQ8zWamtpag7mcG10qs5SMypWR0hkpf4x07OlGtp2VmP2SW-cc0F8DC13g8n8leTcZbiX99YS2DX79TijzISPgEMrnh6GMpsPLx1EwkiP-DRnhjOE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1728259474</pqid></control><display><type>article</type><title>Menstrual blood contains immune cells with inflammatory and anti-inflammatory properties</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Hosseini, Samira ; Shokri, Fazel ; Tokhmechy, Reihaneh ; Savadi-Shiraz, Elham ; Jeddi-Tehrani, Mahmood ; Rahbari, Marjaneh ; Zarnani, Amir-Hassan</creator><creatorcontrib>Hosseini, Samira ; Shokri, Fazel ; Tokhmechy, Reihaneh ; Savadi-Shiraz, Elham ; Jeddi-Tehrani, Mahmood ; Rahbari, Marjaneh ; Zarnani, Amir-Hassan</creatorcontrib><description>Aim
Successful pregnancy requires balanced regulation of immune cells at the feto‐maternal interface. Systemic monitoring of the immune system cannot precisely outline local immune status in the uterus. In this survey, endometrial immune milieu was investigated using a non‐invasive method of analysis of menstrual blood (MB). The results were compared with peripheral blood (PB).
Method
PB and MB of healthy fertile women (n = 15) were collected simultaneously on the second day of their menstrual cycle. T and natural killer T cell subpopulations were immunophenotyped by flow cytometry.
Results
Among examined cell populations, the frequency of CD4 + Foxp3+, CD4 + Foxp3 + CD25‐, CD4 + Foxp3 + CD25+ and IL17+ T cells (P = 0.022, 0.028, 0.017 and 0.005, respectively) and TCRαβ+, CD45RO+, CD16‐, IFNγ + and IL17+ NKT (CD56 + CD3+) cells (P = 0.010, 0.037, 0.038, 0.015 and 0.021, respectively) were significantly higher in MB compared with PB. Conversely, PB contained a higher percentage of CD16+ T cells (P = 0.025) in comparison with MB.
Conclusion
MB contains cells of an inflammatory and anti‐inflammatory nature, implying the existence of finely tuned cell homeostasis during menstruation. Our results imply that MB could be viewed as an easy‐to access specimen for monitoring endometrial immune cells, especially those that have preferential endometrial localization.</description><identifier>ISSN: 1341-8076</identifier><identifier>EISSN: 1447-0756</identifier><identifier>DOI: 10.1111/jog.12801</identifier><identifier>PMID: 26370728</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Adult ; Female ; Flow Cytometry ; Humans ; Immunophenotyping ; Inflammation - blood ; Inflammation - immunology ; interleukin 17 ; Killer Cells, Natural - immunology ; menstrual blood ; Menstruation - blood ; Menstruation - immunology ; NKT cell ; regulatory T cells ; T-Lymphocytes - immunology</subject><ispartof>The journal of obstetrics and gynaecology research, 2015-11, Vol.41 (11), p.1803-1812</ispartof><rights>2015 Japan Society of Obstetrics and Gynecology</rights><rights>2015 Japan Society of Obstetrics and Gynecology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4571-2714079687ef35331fcfae347577093e2479cc787950d47a7ed2a04fc4ee4dea3</citedby><cites>FETCH-LOGICAL-c4571-2714079687ef35331fcfae347577093e2479cc787950d47a7ed2a04fc4ee4dea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjog.12801$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjog.12801$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26370728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hosseini, Samira</creatorcontrib><creatorcontrib>Shokri, Fazel</creatorcontrib><creatorcontrib>Tokhmechy, Reihaneh</creatorcontrib><creatorcontrib>Savadi-Shiraz, Elham</creatorcontrib><creatorcontrib>Jeddi-Tehrani, Mahmood</creatorcontrib><creatorcontrib>Rahbari, Marjaneh</creatorcontrib><creatorcontrib>Zarnani, Amir-Hassan</creatorcontrib><title>Menstrual blood contains immune cells with inflammatory and anti-inflammatory properties</title><title>The journal of obstetrics and gynaecology research</title><addtitle>J. Obstet. Gynaecol. Res</addtitle><description>Aim
Successful pregnancy requires balanced regulation of immune cells at the feto‐maternal interface. Systemic monitoring of the immune system cannot precisely outline local immune status in the uterus. In this survey, endometrial immune milieu was investigated using a non‐invasive method of analysis of menstrual blood (MB). The results were compared with peripheral blood (PB).
Method
PB and MB of healthy fertile women (n = 15) were collected simultaneously on the second day of their menstrual cycle. T and natural killer T cell subpopulations were immunophenotyped by flow cytometry.
Results
Among examined cell populations, the frequency of CD4 + Foxp3+, CD4 + Foxp3 + CD25‐, CD4 + Foxp3 + CD25+ and IL17+ T cells (P = 0.022, 0.028, 0.017 and 0.005, respectively) and TCRαβ+, CD45RO+, CD16‐, IFNγ + and IL17+ NKT (CD56 + CD3+) cells (P = 0.010, 0.037, 0.038, 0.015 and 0.021, respectively) were significantly higher in MB compared with PB. Conversely, PB contained a higher percentage of CD16+ T cells (P = 0.025) in comparison with MB.
Conclusion
MB contains cells of an inflammatory and anti‐inflammatory nature, implying the existence of finely tuned cell homeostasis during menstruation. Our results imply that MB could be viewed as an easy‐to access specimen for monitoring endometrial immune cells, especially those that have preferential endometrial localization.</description><subject>Adult</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Inflammation - blood</subject><subject>Inflammation - immunology</subject><subject>interleukin 17</subject><subject>Killer Cells, Natural - immunology</subject><subject>menstrual blood</subject><subject>Menstruation - blood</subject><subject>Menstruation - immunology</subject><subject>NKT cell</subject><subject>regulatory T cells</subject><subject>T-Lymphocytes - immunology</subject><issn>1341-8076</issn><issn>1447-0756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1PwzAMhiMEgjE48AdQj3DoljRp3R7RgAEbTEJDcIuy1oVAP0bSauzfk7ENiQOWLEfW4zf2S8gJoz3mov9ev_ZYEFO2QzpMCPAphNGue3PB_JhCdEAOrX2nlEHC4n1yEEQcKARxh7zcY2Ub06rCmxV1nXlpXTVKV9bTZdlW6KVYFNZb6ObN01VeqLJUTW2Wnqoyl432_3Tnpp6jaTTaI7KXq8Li8aZ2ydP11XRw448nw9vBxdhPRQjMD4AJCkkUA-Y85Jzlaa6QCwgBaMIxEJCkKcSQhDQToACzQFGRpwJRZKh4l5ytdd3Xny3aRpbarpZWFdatlcydGYSJAOHQ8zWamtpag7mcG10qs5SMypWR0hkpf4x07OlGtp2VmP2SW-cc0F8DC13g8n8leTcZbiX99YS2DX79TijzISPgEMrnh6GMpsPLx1EwkiP-DRnhjOE</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>Hosseini, Samira</creator><creator>Shokri, Fazel</creator><creator>Tokhmechy, Reihaneh</creator><creator>Savadi-Shiraz, Elham</creator><creator>Jeddi-Tehrani, Mahmood</creator><creator>Rahbari, Marjaneh</creator><creator>Zarnani, Amir-Hassan</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201511</creationdate><title>Menstrual blood contains immune cells with inflammatory and anti-inflammatory properties</title><author>Hosseini, Samira ; Shokri, Fazel ; Tokhmechy, Reihaneh ; Savadi-Shiraz, Elham ; Jeddi-Tehrani, Mahmood ; Rahbari, Marjaneh ; Zarnani, Amir-Hassan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4571-2714079687ef35331fcfae347577093e2479cc787950d47a7ed2a04fc4ee4dea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Inflammation - blood</topic><topic>Inflammation - immunology</topic><topic>interleukin 17</topic><topic>Killer Cells, Natural - immunology</topic><topic>menstrual blood</topic><topic>Menstruation - blood</topic><topic>Menstruation - immunology</topic><topic>NKT cell</topic><topic>regulatory T cells</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hosseini, Samira</creatorcontrib><creatorcontrib>Shokri, Fazel</creatorcontrib><creatorcontrib>Tokhmechy, Reihaneh</creatorcontrib><creatorcontrib>Savadi-Shiraz, Elham</creatorcontrib><creatorcontrib>Jeddi-Tehrani, Mahmood</creatorcontrib><creatorcontrib>Rahbari, Marjaneh</creatorcontrib><creatorcontrib>Zarnani, Amir-Hassan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of obstetrics and gynaecology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hosseini, Samira</au><au>Shokri, Fazel</au><au>Tokhmechy, Reihaneh</au><au>Savadi-Shiraz, Elham</au><au>Jeddi-Tehrani, Mahmood</au><au>Rahbari, Marjaneh</au><au>Zarnani, Amir-Hassan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Menstrual blood contains immune cells with inflammatory and anti-inflammatory properties</atitle><jtitle>The journal of obstetrics and gynaecology research</jtitle><addtitle>J. Obstet. Gynaecol. Res</addtitle><date>2015-11</date><risdate>2015</risdate><volume>41</volume><issue>11</issue><spage>1803</spage><epage>1812</epage><pages>1803-1812</pages><issn>1341-8076</issn><eissn>1447-0756</eissn><abstract>Aim
Successful pregnancy requires balanced regulation of immune cells at the feto‐maternal interface. Systemic monitoring of the immune system cannot precisely outline local immune status in the uterus. In this survey, endometrial immune milieu was investigated using a non‐invasive method of analysis of menstrual blood (MB). The results were compared with peripheral blood (PB).
Method
PB and MB of healthy fertile women (n = 15) were collected simultaneously on the second day of their menstrual cycle. T and natural killer T cell subpopulations were immunophenotyped by flow cytometry.
Results
Among examined cell populations, the frequency of CD4 + Foxp3+, CD4 + Foxp3 + CD25‐, CD4 + Foxp3 + CD25+ and IL17+ T cells (P = 0.022, 0.028, 0.017 and 0.005, respectively) and TCRαβ+, CD45RO+, CD16‐, IFNγ + and IL17+ NKT (CD56 + CD3+) cells (P = 0.010, 0.037, 0.038, 0.015 and 0.021, respectively) were significantly higher in MB compared with PB. Conversely, PB contained a higher percentage of CD16+ T cells (P = 0.025) in comparison with MB.
Conclusion
MB contains cells of an inflammatory and anti‐inflammatory nature, implying the existence of finely tuned cell homeostasis during menstruation. Our results imply that MB could be viewed as an easy‐to access specimen for monitoring endometrial immune cells, especially those that have preferential endometrial localization.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>26370728</pmid><doi>10.1111/jog.12801</doi><tpages>10</tpages></addata></record> |
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| subjects | Adult Female Flow Cytometry Humans Immunophenotyping Inflammation - blood Inflammation - immunology interleukin 17 Killer Cells, Natural - immunology menstrual blood Menstruation - blood Menstruation - immunology NKT cell regulatory T cells T-Lymphocytes - immunology |
| title | Menstrual blood contains immune cells with inflammatory and anti-inflammatory properties |
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