Circulating microRNAs in obese and lean heart failure patients: A case–control study with computational target prediction analysis
MicroRNAs (miRs) regulate processes involved in both cardiac remodeling and obesity. We investigated if the expression of selected miRs in patients with heart failure (HF) is influenced by the presence of obesity. In this case–control study, we compared plasma levels of miR-21, -130b, -221, -423-5p,...
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| Veröffentlicht in: | Gene 2015-12, Vol.574 (1), p.1-10 |
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| creator | Thomé, Juliana Gil Mendoza, Mariana Recamonde Cheuiche, Amanda Veiga La Porta, Vanessa Laubert Silvello, Daiane dos Santos, Kátia Gonçalves Andrades, Michael Everton Clausell, Nadine Rohde, Luis Eduardo Biolo, Andréia |
| description | MicroRNAs (miRs) regulate processes involved in both cardiac remodeling and obesity. We investigated if the expression of selected miRs in patients with heart failure (HF) is influenced by the presence of obesity.
In this case–control study, we compared plasma levels of miR-21, -130b, -221, -423-5p, and the -221/-130b ratio in 57 age- and gender-matched subjects: 40 HF patients (20 obese HF and 20 lean HF) and 17 lean healthy controls. Body composition was estimated by bioelectrical impedance analysis. MiRs were measured by quantitative reverse transcription-PCR. Bioinformatics analysis was performed based on miRs findings to predict their putative targets and investigate their biological function.
HF was associated with increased miR-423-5p levels in both lean and obese patients (P |
| doi_str_mv | 10.1016/j.gene.2015.07.068 |
| format | Article |
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In this case–control study, we compared plasma levels of miR-21, -130b, -221, -423-5p, and the -221/-130b ratio in 57 age- and gender-matched subjects: 40 HF patients (20 obese HF and 20 lean HF) and 17 lean healthy controls. Body composition was estimated by bioelectrical impedance analysis. MiRs were measured by quantitative reverse transcription-PCR. Bioinformatics analysis was performed based on miRs findings to predict their putative targets and investigate their biological function.
HF was associated with increased miR-423-5p levels in both lean and obese patients (P<0.05 vs. controls) without differences between HF groups. MiR-130b levels were reduced in obese HF patients compared with HF lean (P=0.036) and controls (P=0.025). MiR-221 levels were non-significantly increased in obese HF patients. MiR-21 levels were not different among the groups. MiR-221/-130b ratio was increased in obese HF patients, and was positively associated with body fat percentage (r=0.43; P=0.002), body mass index (r=0.44; P=0.002), and waist circumference (r=0.40; P=0.020). Computational prediction of target genes followed by functional enrichment analysis indicated a relevant role of miR-130b and miR-221 in modulating the expression of genes associated to cardiovascular and endocrine diseases, and suggested their influence in important signaling mechanisms and in numerous processes related to the circulatory and endocrine systems.
In HF patients, the presence of obesity is associated with a differential expression of selected miRs and the miR-221/-130b ratio had significant correlations with adiposity parameters. Computational target prediction analysis identified several interrelated pathways targeted by miR-130b and miR-221 with a known relationship with endocrine and cardiovascular diseases, representing potential mechanisms to be further validated.
•In HF patients, obesity is associated with altered expression of miR-130b and miR-221.•The ratio miR-221/-130b has positive correlation with adiposity parameters.•Predicted miR targets are highly enriched in cardiovascular and endocrine diseases.•Bioinformatics analysis reveals that these miRs modulate important signaling pathways.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2015.07.068</identifier><identifier>PMID: 26211628</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adiposity ; Body Composition - genetics ; Body Mass Index ; Case-Control Studies ; Computational Biology - methods ; Female ; Heart failure ; Heart Failure - blood ; Heart Failure - genetics ; Humans ; Male ; MicroRNA ; MicroRNAs - blood ; MicroRNAs - genetics ; Middle Aged ; Obesity ; Obesity - blood ; Obesity - genetics ; Target prediction ; Thinness - blood ; Thinness - genetics ; Waist Circumference - genetics</subject><ispartof>Gene, 2015-12, Vol.574 (1), p.1-10</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-239ad00f12cffc12c61b56dae856a57da59d8fe2203f264e03af7f285a7973653</citedby><cites>FETCH-LOGICAL-c433t-239ad00f12cffc12c61b56dae856a57da59d8fe2203f264e03af7f285a7973653</cites><orcidid>0000-0001-5231-2071</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2015.07.068$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26211628$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomé, Juliana Gil</creatorcontrib><creatorcontrib>Mendoza, Mariana Recamonde</creatorcontrib><creatorcontrib>Cheuiche, Amanda Veiga</creatorcontrib><creatorcontrib>La Porta, Vanessa Laubert</creatorcontrib><creatorcontrib>Silvello, Daiane</creatorcontrib><creatorcontrib>dos Santos, Kátia Gonçalves</creatorcontrib><creatorcontrib>Andrades, Michael Everton</creatorcontrib><creatorcontrib>Clausell, Nadine</creatorcontrib><creatorcontrib>Rohde, Luis Eduardo</creatorcontrib><creatorcontrib>Biolo, Andréia</creatorcontrib><title>Circulating microRNAs in obese and lean heart failure patients: A case–control study with computational target prediction analysis</title><title>Gene</title><addtitle>Gene</addtitle><description>MicroRNAs (miRs) regulate processes involved in both cardiac remodeling and obesity. We investigated if the expression of selected miRs in patients with heart failure (HF) is influenced by the presence of obesity.
In this case–control study, we compared plasma levels of miR-21, -130b, -221, -423-5p, and the -221/-130b ratio in 57 age- and gender-matched subjects: 40 HF patients (20 obese HF and 20 lean HF) and 17 lean healthy controls. Body composition was estimated by bioelectrical impedance analysis. MiRs were measured by quantitative reverse transcription-PCR. Bioinformatics analysis was performed based on miRs findings to predict their putative targets and investigate their biological function.
HF was associated with increased miR-423-5p levels in both lean and obese patients (P<0.05 vs. controls) without differences between HF groups. MiR-130b levels were reduced in obese HF patients compared with HF lean (P=0.036) and controls (P=0.025). MiR-221 levels were non-significantly increased in obese HF patients. MiR-21 levels were not different among the groups. MiR-221/-130b ratio was increased in obese HF patients, and was positively associated with body fat percentage (r=0.43; P=0.002), body mass index (r=0.44; P=0.002), and waist circumference (r=0.40; P=0.020). Computational prediction of target genes followed by functional enrichment analysis indicated a relevant role of miR-130b and miR-221 in modulating the expression of genes associated to cardiovascular and endocrine diseases, and suggested their influence in important signaling mechanisms and in numerous processes related to the circulatory and endocrine systems.
In HF patients, the presence of obesity is associated with a differential expression of selected miRs and the miR-221/-130b ratio had significant correlations with adiposity parameters. Computational target prediction analysis identified several interrelated pathways targeted by miR-130b and miR-221 with a known relationship with endocrine and cardiovascular diseases, representing potential mechanisms to be further validated.
•In HF patients, obesity is associated with altered expression of miR-130b and miR-221.•The ratio miR-221/-130b has positive correlation with adiposity parameters.•Predicted miR targets are highly enriched in cardiovascular and endocrine diseases.•Bioinformatics analysis reveals that these miRs modulate important signaling pathways.</description><subject>Adiposity</subject><subject>Body Composition - genetics</subject><subject>Body Mass Index</subject><subject>Case-Control Studies</subject><subject>Computational Biology - methods</subject><subject>Female</subject><subject>Heart failure</subject><subject>Heart Failure - blood</subject><subject>Heart Failure - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>MicroRNA</subject><subject>MicroRNAs - blood</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>Obesity</subject><subject>Obesity - blood</subject><subject>Obesity - genetics</subject><subject>Target prediction</subject><subject>Thinness - blood</subject><subject>Thinness - genetics</subject><subject>Waist Circumference - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAYRS0EotPCC7BAXrJJsJ3xTxCb0QgKUkWlCtaWx_k89Shxgu0UzY4Fb9A35ElwNKVLhBe2ZN17Fvcg9IqSmhIq3h7qPQSoGaG8JrImQj1BK6pkWxHSqKdoRRqpKkppe4bOUzqQcjhnz9EZE4xSwdQK_dr6aOfeZB_2ePA2jjdfNgn7gMcdJMAmdLgHE_AtmJixM76fI-CpFCDk9A5vsDUJfv-8t2PIcexxynN3xD98vsV2HKY5l-gYTI-ziXvIeIrQebv8Fbjpj8mnF-iZM32Clw_vBfr28cPX7afq6vry83ZzVdl10-SKNa3pCHGUWedsuQXdcdEZUFwYLjvD2045YIw0jok1kMY46ZjiRrayEby5QG9O3CmO32dIWQ8-Weh7E2Cck6aSSdEqLun_RGlLFV0vVHaKlvFSiuD0FP1g4lFTohdR-qAXUXoRpYnURVQpvX7gz7sBusfKXzMl8P4UgDLInYeoky2T2zJeBJt1N_p_8f8AAnynMw</recordid><startdate>20151210</startdate><enddate>20151210</enddate><creator>Thomé, Juliana Gil</creator><creator>Mendoza, Mariana Recamonde</creator><creator>Cheuiche, Amanda Veiga</creator><creator>La Porta, Vanessa Laubert</creator><creator>Silvello, Daiane</creator><creator>dos Santos, Kátia Gonçalves</creator><creator>Andrades, Michael Everton</creator><creator>Clausell, Nadine</creator><creator>Rohde, Luis Eduardo</creator><creator>Biolo, Andréia</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0001-5231-2071</orcidid></search><sort><creationdate>20151210</creationdate><title>Circulating microRNAs in obese and lean heart failure patients: A case–control study with computational target prediction analysis</title><author>Thomé, Juliana Gil ; Mendoza, Mariana Recamonde ; Cheuiche, Amanda Veiga ; La Porta, Vanessa Laubert ; Silvello, Daiane ; dos Santos, Kátia Gonçalves ; Andrades, Michael Everton ; Clausell, Nadine ; Rohde, Luis Eduardo ; Biolo, Andréia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-239ad00f12cffc12c61b56dae856a57da59d8fe2203f264e03af7f285a7973653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adiposity</topic><topic>Body Composition - genetics</topic><topic>Body Mass Index</topic><topic>Case-Control Studies</topic><topic>Computational Biology - methods</topic><topic>Female</topic><topic>Heart failure</topic><topic>Heart Failure - blood</topic><topic>Heart Failure - genetics</topic><topic>Humans</topic><topic>Male</topic><topic>MicroRNA</topic><topic>MicroRNAs - blood</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>Obesity</topic><topic>Obesity - blood</topic><topic>Obesity - genetics</topic><topic>Target prediction</topic><topic>Thinness - blood</topic><topic>Thinness - genetics</topic><topic>Waist Circumference - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomé, Juliana Gil</creatorcontrib><creatorcontrib>Mendoza, Mariana Recamonde</creatorcontrib><creatorcontrib>Cheuiche, Amanda Veiga</creatorcontrib><creatorcontrib>La Porta, Vanessa Laubert</creatorcontrib><creatorcontrib>Silvello, Daiane</creatorcontrib><creatorcontrib>dos Santos, Kátia Gonçalves</creatorcontrib><creatorcontrib>Andrades, Michael Everton</creatorcontrib><creatorcontrib>Clausell, Nadine</creatorcontrib><creatorcontrib>Rohde, Luis Eduardo</creatorcontrib><creatorcontrib>Biolo, Andréia</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomé, Juliana Gil</au><au>Mendoza, Mariana Recamonde</au><au>Cheuiche, Amanda Veiga</au><au>La Porta, Vanessa Laubert</au><au>Silvello, Daiane</au><au>dos Santos, Kátia Gonçalves</au><au>Andrades, Michael Everton</au><au>Clausell, Nadine</au><au>Rohde, Luis Eduardo</au><au>Biolo, Andréia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating microRNAs in obese and lean heart failure patients: A case–control study with computational target prediction analysis</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2015-12-10</date><risdate>2015</risdate><volume>574</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>MicroRNAs (miRs) regulate processes involved in both cardiac remodeling and obesity. We investigated if the expression of selected miRs in patients with heart failure (HF) is influenced by the presence of obesity.
In this case–control study, we compared plasma levels of miR-21, -130b, -221, -423-5p, and the -221/-130b ratio in 57 age- and gender-matched subjects: 40 HF patients (20 obese HF and 20 lean HF) and 17 lean healthy controls. Body composition was estimated by bioelectrical impedance analysis. MiRs were measured by quantitative reverse transcription-PCR. Bioinformatics analysis was performed based on miRs findings to predict their putative targets and investigate their biological function.
HF was associated with increased miR-423-5p levels in both lean and obese patients (P<0.05 vs. controls) without differences between HF groups. MiR-130b levels were reduced in obese HF patients compared with HF lean (P=0.036) and controls (P=0.025). MiR-221 levels were non-significantly increased in obese HF patients. MiR-21 levels were not different among the groups. MiR-221/-130b ratio was increased in obese HF patients, and was positively associated with body fat percentage (r=0.43; P=0.002), body mass index (r=0.44; P=0.002), and waist circumference (r=0.40; P=0.020). Computational prediction of target genes followed by functional enrichment analysis indicated a relevant role of miR-130b and miR-221 in modulating the expression of genes associated to cardiovascular and endocrine diseases, and suggested their influence in important signaling mechanisms and in numerous processes related to the circulatory and endocrine systems.
In HF patients, the presence of obesity is associated with a differential expression of selected miRs and the miR-221/-130b ratio had significant correlations with adiposity parameters. Computational target prediction analysis identified several interrelated pathways targeted by miR-130b and miR-221 with a known relationship with endocrine and cardiovascular diseases, representing potential mechanisms to be further validated.
•In HF patients, obesity is associated with altered expression of miR-130b and miR-221.•The ratio miR-221/-130b has positive correlation with adiposity parameters.•Predicted miR targets are highly enriched in cardiovascular and endocrine diseases.•Bioinformatics analysis reveals that these miRs modulate important signaling pathways.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26211628</pmid><doi>10.1016/j.gene.2015.07.068</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5231-2071</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adiposity Body Composition - genetics Body Mass Index Case-Control Studies Computational Biology - methods Female Heart failure Heart Failure - blood Heart Failure - genetics Humans Male MicroRNA MicroRNAs - blood MicroRNAs - genetics Middle Aged Obesity Obesity - blood Obesity - genetics Target prediction Thinness - blood Thinness - genetics Waist Circumference - genetics |
| title | Circulating microRNAs in obese and lean heart failure patients: A case–control study with computational target prediction analysis |
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