Effect of varicocelectomy on sperm functional characteristics and DNA methylation
Summary In individuals with varicocele, DNA is damaged due to high level of oxidative stress, and varicocelectomy can overcome this effect. Damaged DNA is less liable to DNA methylation, and antioxidant therapy appears to have the potential to reduce sperm oxidative stress and DNA damage and thereby...
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| Veröffentlicht in: | Andrologia 2015-10, Vol.47 (8), p.904-909 |
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| creator | Tavalaee, M. Bahreinian, M. Barekat, F. Abbasi, H. Nasr-Esfahani, M. H. |
| description | Summary
In individuals with varicocele, DNA is damaged due to high level of oxidative stress, and varicocelectomy can overcome this effect. Damaged DNA is less liable to DNA methylation, and antioxidant therapy appears to have the potential to reduce sperm oxidative stress and DNA damage and thereby maintain DNA methylation, while effect of varicocelectomy on DNA methylation patterns has remained unclear. In the light of these considerations, we aimed to examine the effect of varicocelectomy on sperm DNA methylation and functional characteristics. Fifty‐two men with left‐sided varicocele (grade II &III) were included. Sperm parameters, DNA fragmentation, protamine deficiency, oxidative stress and global DNA methylation were evaluated before and 3 months after surgery. Our data show that sperm concentration, percentages of spermatozoon with abnormal morphology, DNA fragmentation, protamine deficiency and oxidative stress significantly improved after surgery. Percentage of sperm motility, global DNA methylation and intensity of DNA methylation also improved after surgery, although the differences were not significant when compared with before surgery. Categorisation of individuals to subgroups revealed that improvement of DNA methylation appears to take place in oligozoospermic individuals, which are more severely affected by state of varicocele. However, this is a preliminary study, and further studies are required to solidify this conclusion. |
| doi_str_mv | 10.1111/and.12345 |
| format | Article |
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In individuals with varicocele, DNA is damaged due to high level of oxidative stress, and varicocelectomy can overcome this effect. Damaged DNA is less liable to DNA methylation, and antioxidant therapy appears to have the potential to reduce sperm oxidative stress and DNA damage and thereby maintain DNA methylation, while effect of varicocelectomy on DNA methylation patterns has remained unclear. In the light of these considerations, we aimed to examine the effect of varicocelectomy on sperm DNA methylation and functional characteristics. Fifty‐two men with left‐sided varicocele (grade II &III) were included. Sperm parameters, DNA fragmentation, protamine deficiency, oxidative stress and global DNA methylation were evaluated before and 3 months after surgery. Our data show that sperm concentration, percentages of spermatozoon with abnormal morphology, DNA fragmentation, protamine deficiency and oxidative stress significantly improved after surgery. Percentage of sperm motility, global DNA methylation and intensity of DNA methylation also improved after surgery, although the differences were not significant when compared with before surgery. Categorisation of individuals to subgroups revealed that improvement of DNA methylation appears to take place in oligozoospermic individuals, which are more severely affected by state of varicocele. However, this is a preliminary study, and further studies are required to solidify this conclusion.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/and.12345</identifier><identifier>PMID: 25234073</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Adult ; DNA Fragmentation ; DNA methylation ; DNA Methylation - physiology ; Flow Cytometry ; Humans ; In Situ Nick-End Labeling ; Male ; Middle Aged ; Oxidative Stress ; protamine deficiency sperm parameters ; Spermatic Cord - surgery ; Spermatozoa - abnormalities ; Spermatozoa - metabolism ; Spermatozoa - physiology ; Varicocele - surgery ; varicocelectomy ; Young Adult</subject><ispartof>Andrologia, 2015-10, Vol.47 (8), p.904-909</ispartof><rights>2014 Blackwell Verlag GmbH</rights><rights>2014 Blackwell Verlag GmbH.</rights><rights>Copyright © 2015 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4595-a3ff8e05f960b0cd09abac3db3a8b9b707b5959f39d39b98fd5aae630e0c3b233</citedby><cites>FETCH-LOGICAL-c4595-a3ff8e05f960b0cd09abac3db3a8b9b707b5959f39d39b98fd5aae630e0c3b233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fand.12345$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fand.12345$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25234073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tavalaee, M.</creatorcontrib><creatorcontrib>Bahreinian, M.</creatorcontrib><creatorcontrib>Barekat, F.</creatorcontrib><creatorcontrib>Abbasi, H.</creatorcontrib><creatorcontrib>Nasr-Esfahani, M. H.</creatorcontrib><title>Effect of varicocelectomy on sperm functional characteristics and DNA methylation</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Summary
In individuals with varicocele, DNA is damaged due to high level of oxidative stress, and varicocelectomy can overcome this effect. Damaged DNA is less liable to DNA methylation, and antioxidant therapy appears to have the potential to reduce sperm oxidative stress and DNA damage and thereby maintain DNA methylation, while effect of varicocelectomy on DNA methylation patterns has remained unclear. In the light of these considerations, we aimed to examine the effect of varicocelectomy on sperm DNA methylation and functional characteristics. Fifty‐two men with left‐sided varicocele (grade II &III) were included. Sperm parameters, DNA fragmentation, protamine deficiency, oxidative stress and global DNA methylation were evaluated before and 3 months after surgery. Our data show that sperm concentration, percentages of spermatozoon with abnormal morphology, DNA fragmentation, protamine deficiency and oxidative stress significantly improved after surgery. Percentage of sperm motility, global DNA methylation and intensity of DNA methylation also improved after surgery, although the differences were not significant when compared with before surgery. Categorisation of individuals to subgroups revealed that improvement of DNA methylation appears to take place in oligozoospermic individuals, which are more severely affected by state of varicocele. However, this is a preliminary study, and further studies are required to solidify this conclusion.</description><subject>Adult</subject><subject>DNA Fragmentation</subject><subject>DNA methylation</subject><subject>DNA Methylation - physiology</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Oxidative Stress</subject><subject>protamine deficiency sperm parameters</subject><subject>Spermatic Cord - surgery</subject><subject>Spermatozoa - abnormalities</subject><subject>Spermatozoa - metabolism</subject><subject>Spermatozoa - physiology</subject><subject>Varicocele - surgery</subject><subject>varicocelectomy</subject><subject>Young Adult</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1O3DAUha2qVRlRFrwAssSmXQRucuM4Xg6_rUCDWrViadmOLQJJPLWTlnn7ehpgUakSd2NZ-s7nn0PIfg5HeZpjNTRHeYEle0MWeYkig4IXb8kCEDArWSV2yF6M95CmZJyX5XuyU7AUAI4L8vXcOWtG6h39pUJrvLFd2vt-Q_1A49qGnrppMGPrB9VRc6eCMqMNbRxbE2k6nJ6tlrS3492mU1vqA3nnVBft3tO6S35cnH8__Zxd31x-OV1eZ6ZkgmUKnastMCcq0GAaEEorg41GVWuhOXCdMOFQNCi0qF3DlLIVggWDukDcJR9n7zr4n5ONo-zbmG7fqcH6KcqcF7wSAIy9AgXB8wrZ1nr4D3rvp5CePlMMa8x5oj7NlAk-xmCdXIe2V2Ejc5DbVmT6GPm3lcQePBkn3dvmhXzuIAHHM_C77ezm_ya5XJ09K7M5kVqwjy8JFR5kxZEzebu6lOykWF3V7Ep-wz9WjqQ1</recordid><startdate>201510</startdate><enddate>201510</enddate><creator>Tavalaee, M.</creator><creator>Bahreinian, M.</creator><creator>Barekat, F.</creator><creator>Abbasi, H.</creator><creator>Nasr-Esfahani, M. H.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201510</creationdate><title>Effect of varicocelectomy on sperm functional characteristics and DNA methylation</title><author>Tavalaee, M. ; Bahreinian, M. ; Barekat, F. ; Abbasi, H. ; Nasr-Esfahani, M. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4595-a3ff8e05f960b0cd09abac3db3a8b9b707b5959f39d39b98fd5aae630e0c3b233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>DNA Fragmentation</topic><topic>DNA methylation</topic><topic>DNA Methylation - physiology</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Oxidative Stress</topic><topic>protamine deficiency sperm parameters</topic><topic>Spermatic Cord - surgery</topic><topic>Spermatozoa - abnormalities</topic><topic>Spermatozoa - metabolism</topic><topic>Spermatozoa - physiology</topic><topic>Varicocele - surgery</topic><topic>varicocelectomy</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tavalaee, M.</creatorcontrib><creatorcontrib>Bahreinian, M.</creatorcontrib><creatorcontrib>Barekat, F.</creatorcontrib><creatorcontrib>Abbasi, H.</creatorcontrib><creatorcontrib>Nasr-Esfahani, M. H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tavalaee, M.</au><au>Bahreinian, M.</au><au>Barekat, F.</au><au>Abbasi, H.</au><au>Nasr-Esfahani, M. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of varicocelectomy on sperm functional characteristics and DNA methylation</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2015-10</date><risdate>2015</risdate><volume>47</volume><issue>8</issue><spage>904</spage><epage>909</epage><pages>904-909</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Summary
In individuals with varicocele, DNA is damaged due to high level of oxidative stress, and varicocelectomy can overcome this effect. Damaged DNA is less liable to DNA methylation, and antioxidant therapy appears to have the potential to reduce sperm oxidative stress and DNA damage and thereby maintain DNA methylation, while effect of varicocelectomy on DNA methylation patterns has remained unclear. In the light of these considerations, we aimed to examine the effect of varicocelectomy on sperm DNA methylation and functional characteristics. Fifty‐two men with left‐sided varicocele (grade II &III) were included. Sperm parameters, DNA fragmentation, protamine deficiency, oxidative stress and global DNA methylation were evaluated before and 3 months after surgery. Our data show that sperm concentration, percentages of spermatozoon with abnormal morphology, DNA fragmentation, protamine deficiency and oxidative stress significantly improved after surgery. Percentage of sperm motility, global DNA methylation and intensity of DNA methylation also improved after surgery, although the differences were not significant when compared with before surgery. Categorisation of individuals to subgroups revealed that improvement of DNA methylation appears to take place in oligozoospermic individuals, which are more severely affected by state of varicocele. However, this is a preliminary study, and further studies are required to solidify this conclusion.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>25234073</pmid><doi>10.1111/and.12345</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult DNA Fragmentation DNA methylation DNA Methylation - physiology Flow Cytometry Humans In Situ Nick-End Labeling Male Middle Aged Oxidative Stress protamine deficiency sperm parameters Spermatic Cord - surgery Spermatozoa - abnormalities Spermatozoa - metabolism Spermatozoa - physiology Varicocele - surgery varicocelectomy Young Adult |
| title | Effect of varicocelectomy on sperm functional characteristics and DNA methylation |
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