High yield expression of novel glutaminase free l-asparaginase II of Pectobacterium carotovorum MTCC 1428 in Bacillus subtilis WB800N

Gene encoding glutaminase-free l -asparaginase II ( ans B2 ) from Pectobacterium carotovorum MTCC 1428 was cloned into pHT43, transformed in Bacillus subtilis WB800N and optimised the expression levels of recombinant enzyme. A three-fold higher enzyme production was observed with an efficient transf...

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Veröffentlicht in:	Bioprocess and biosystems engineering 2015-11, Vol.38 (11), p.2271-2284
Hauptverfasser:	Chityala, Sushma, Venkata Dasu, Veeranki, Ahmad, Jamal, Prakasham, Reddy Shetty
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Asparaginase - biosynthesis Asparaginase - genetics Bacillus subtilis Bacillus subtilis - genetics Bacillus subtilis - metabolism Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Biotechnology Chemistry Chemistry and Materials Science Environmental Engineering/Biotechnology Food Science Gene Expression Genes Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Membranes Original Paper Pectobacterium Pectobacterium carotovorum - enzymology Pectobacterium carotovorum - genetics Recombinant Proteins - biosynthesis Recombinant Proteins - genetics
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