Air samplings in a Campylobacter jejuni positive laying hen flock
The air in laying hen houses contains high concentrations of airborne bacteria. The numbers of these bacteria can be influenced by the efficiency of the chosen sampling method. In the presented study, AGI-30 Impingers and the Coriolis(®)µ air Sampler were compared in terms of their efficiency in sam...
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| description | The air in laying hen houses contains high concentrations of airborne bacteria. The numbers of these bacteria can be influenced by the efficiency of the chosen sampling method. In the presented study, AGI-30 Impingers and the Coriolis(®)µ air Sampler were compared in terms of their efficiency in sampling aerobic mesophilic bacteria in a laying hen house. Measurements were conducted in a laying hen flock with high prevalences of C. jejuni in order to investigate if culturable cells of this organism can also be detected by the applied methods. Airborne dust was also analyzed for the presence of C. jejuni specific DNA to assess the possible occurrence of non-culturable C. jejuni in the hen house air. The numbers of mesophilic airborne bacteria ranged from 8 × 10(4) - 2 × 10(6) CFU/m(-3) when sampled using AGI-30 Impingers, and from 2 × 10(5) - 4 × 10(6) CFU/m -3 when sampled using a Coriolis(®)µ air Sampler. The concentrations detected simultaneously by both devices correlated well (rPearson = 0.755), but the Coriolis(®)µ air Sampler showed a significantly higher sampling efficiency (p |
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The numbers of these bacteria can be influenced by the efficiency of the chosen sampling method. In the presented study, AGI-30 Impingers and the Coriolis(®)µ air Sampler were compared in terms of their efficiency in sampling aerobic mesophilic bacteria in a laying hen house. Measurements were conducted in a laying hen flock with high prevalences of C. jejuni in order to investigate if culturable cells of this organism can also be detected by the applied methods. Airborne dust was also analyzed for the presence of C. jejuni specific DNA to assess the possible occurrence of non-culturable C. jejuni in the hen house air. The numbers of mesophilic airborne bacteria ranged from 8 × 10(4) - 2 × 10(6) CFU/m(-3) when sampled using AGI-30 Impingers, and from 2 × 10(5) - 4 × 10(6) CFU/m -3 when sampled using a Coriolis(®)µ air Sampler. The concentrations detected simultaneously by both devices correlated well (rPearson = 0.755), but the Coriolis(®)µ air Sampler showed a significantly higher sampling efficiency (p<0.001). Although, the within flock prevalence of C. jejuni was high during the experiments (between 70-93%), neither of the air sampling methods could detect culturable C. jejuni from the air. However, C. jejuni specific DNA was detected in 15 out of 18 airborne dust samples by mapA PCR. Based on the results, it can be concluded that airborne culturable C. jejuni were not detectable, even with an efficient air sampler, because of their low concentration. Therefore, the risk of airborne infection to poultry workers on inhaling airborne C. jejuni seems negligible. Also, the transmission of culturable C. jejuni to neighboring farms by the airborne route is unlikely. Otherwise, the detection of airborne C. jejuni specific DNA suggests that non-culturable cells could appear in the hen house air, and in future it should be verified whether sampling stress of the air sampling methods could induce the non-culturable state.</description><identifier>ISSN: 1232-1966</identifier><identifier>EISSN: 1898-2263</identifier><identifier>PMID: 23540207</identifier><language>eng</language><publisher>Poland: Institute of Rural Health</publisher><subject>Air Microbiology ; Air sampling ; Airborne bacteria ; Airborne microorganisms ; Animals ; Bacteria ; Campylobacter ; Campylobacter jejuni ; Campylobacter jejuni - isolation & purification ; Chickens - microbiology ; Deoxyribonucleic acid ; DNA ; Dust ; Efficiency ; Farms ; Female ; Housing, Animal ; Mesophilic bacteria ; Oviposition ; Sampling methods ; Time Factors</subject><ispartof>Annals of Agricultural and Environmental Medicine, 2013, Vol.20 (1), p.16-20</ispartof><rights>2013. This work is published under https://creativecommons.org/licenses/by-nc/3.0/pl/deed.en (the “License”). 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The numbers of these bacteria can be influenced by the efficiency of the chosen sampling method. In the presented study, AGI-30 Impingers and the Coriolis(®)µ air Sampler were compared in terms of their efficiency in sampling aerobic mesophilic bacteria in a laying hen house. Measurements were conducted in a laying hen flock with high prevalences of C. jejuni in order to investigate if culturable cells of this organism can also be detected by the applied methods. Airborne dust was also analyzed for the presence of C. jejuni specific DNA to assess the possible occurrence of non-culturable C. jejuni in the hen house air. The numbers of mesophilic airborne bacteria ranged from 8 × 10(4) - 2 × 10(6) CFU/m(-3) when sampled using AGI-30 Impingers, and from 2 × 10(5) - 4 × 10(6) CFU/m -3 when sampled using a Coriolis(®)µ air Sampler. The concentrations detected simultaneously by both devices correlated well (rPearson = 0.755), but the Coriolis(®)µ air Sampler showed a significantly higher sampling efficiency (p<0.001). Although, the within flock prevalence of C. jejuni was high during the experiments (between 70-93%), neither of the air sampling methods could detect culturable C. jejuni from the air. However, C. jejuni specific DNA was detected in 15 out of 18 airborne dust samples by mapA PCR. Based on the results, it can be concluded that airborne culturable C. jejuni were not detectable, even with an efficient air sampler, because of their low concentration. Therefore, the risk of airborne infection to poultry workers on inhaling airborne C. jejuni seems negligible. Also, the transmission of culturable C. jejuni to neighboring farms by the airborne route is unlikely. 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Schulz, Jochen ; Hartung, Joerg</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p272t-31e8bc860296076b244351f87cf973ee85197efe44e03b6a75f0c76fb713663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Air Microbiology</topic><topic>Air sampling</topic><topic>Airborne bacteria</topic><topic>Airborne microorganisms</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Campylobacter</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>Chickens - microbiology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Dust</topic><topic>Efficiency</topic><topic>Farms</topic><topic>Female</topic><topic>Housing, Animal</topic><topic>Mesophilic bacteria</topic><topic>Oviposition</topic><topic>Sampling methods</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahmed, Marwa Fawzy El Metwaly</creatorcontrib><creatorcontrib>Schulz, Jochen</creatorcontrib><creatorcontrib>Hartung, Joerg</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Pollution Abstracts</collection><collection>Risk Abstracts</collection><collection>Safety Science and Risk</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Annals of Agricultural and Environmental Medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahmed, Marwa Fawzy El Metwaly</au><au>Schulz, Jochen</au><au>Hartung, Joerg</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Air samplings in a Campylobacter jejuni positive laying hen flock</atitle><jtitle>Annals of Agricultural and Environmental Medicine</jtitle><addtitle>Ann Agric Environ Med</addtitle><date>2013</date><risdate>2013</risdate><volume>20</volume><issue>1</issue><spage>16</spage><epage>20</epage><pages>16-20</pages><issn>1232-1966</issn><eissn>1898-2263</eissn><abstract>The air in laying hen houses contains high concentrations of airborne bacteria. The numbers of these bacteria can be influenced by the efficiency of the chosen sampling method. In the presented study, AGI-30 Impingers and the Coriolis(®)µ air Sampler were compared in terms of their efficiency in sampling aerobic mesophilic bacteria in a laying hen house. Measurements were conducted in a laying hen flock with high prevalences of C. jejuni in order to investigate if culturable cells of this organism can also be detected by the applied methods. Airborne dust was also analyzed for the presence of C. jejuni specific DNA to assess the possible occurrence of non-culturable C. jejuni in the hen house air. The numbers of mesophilic airborne bacteria ranged from 8 × 10(4) - 2 × 10(6) CFU/m(-3) when sampled using AGI-30 Impingers, and from 2 × 10(5) - 4 × 10(6) CFU/m -3 when sampled using a Coriolis(®)µ air Sampler. The concentrations detected simultaneously by both devices correlated well (rPearson = 0.755), but the Coriolis(®)µ air Sampler showed a significantly higher sampling efficiency (p<0.001). Although, the within flock prevalence of C. jejuni was high during the experiments (between 70-93%), neither of the air sampling methods could detect culturable C. jejuni from the air. However, C. jejuni specific DNA was detected in 15 out of 18 airborne dust samples by mapA PCR. Based on the results, it can be concluded that airborne culturable C. jejuni were not detectable, even with an efficient air sampler, because of their low concentration. Therefore, the risk of airborne infection to poultry workers on inhaling airborne C. jejuni seems negligible. Also, the transmission of culturable C. jejuni to neighboring farms by the airborne route is unlikely. Otherwise, the detection of airborne C. jejuni specific DNA suggests that non-culturable cells could appear in the hen house air, and in future it should be verified whether sampling stress of the air sampling methods could induce the non-culturable state.</abstract><cop>Poland</cop><pub>Institute of Rural Health</pub><pmid>23540207</pmid><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Air Microbiology Air sampling Airborne bacteria Airborne microorganisms Animals Bacteria Campylobacter Campylobacter jejuni Campylobacter jejuni - isolation & purification Chickens - microbiology Deoxyribonucleic acid DNA Dust Efficiency Farms Female Housing, Animal Mesophilic bacteria Oviposition Sampling methods Time Factors |
| title | Air samplings in a Campylobacter jejuni positive laying hen flock |
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