Circulating brain derived neurotrophic factor (BDNF) and frequency of BDNF positive T cells in peripheral blood in human ischemic stroke: Effect on outcome
Abstract The aim of this study was to measure the levels of circulating BDNF and the frequency of BDNF-producing T cells after acute ischaemic stroke. Serum BDNF levels were measured by ELISA. Flow cytometry was used to enumerate peripheral blood leukocytes that were labelled with antibodies against...
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| Veröffentlicht in: | Journal of neuroimmunology 2015-09, Vol.286, p.42-47 |
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| creator | Chan, Adeline Yan, Jun Csurhes, Peter Greer, Judith McCombe, Pamela |
| description | Abstract The aim of this study was to measure the levels of circulating BDNF and the frequency of BDNF-producing T cells after acute ischaemic stroke. Serum BDNF levels were measured by ELISA. Flow cytometry was used to enumerate peripheral blood leukocytes that were labelled with antibodies against markers of T cells, T regulatory cells (Tregs), and intracellular BDNF. There was a slight increase in serum BDNF levels after stroke. There was no overall difference between stroke patients and controls in the frequency of CD4+ and CD8+ BDNF+ cells, although a subgroup of stroke patients showed high frequencies of these cells. However, there was an increase in the percentage of BDNF+ Treg cells in the CD4+ population in stroke patients compared to controls. Patients with high percentages of CD4+ BDNF+ Treg cells had a better outcome at 6 months than those with lower levels. These groups did not differ in age, gender or initial stroke severity. Enhancement of BDNF production after stroke could be a useful means of improving neuroprotection and recovery after stroke. |
| doi_str_mv | 10.1016/j.jneuroim.2015.06.013 |
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Serum BDNF levels were measured by ELISA. Flow cytometry was used to enumerate peripheral blood leukocytes that were labelled with antibodies against markers of T cells, T regulatory cells (Tregs), and intracellular BDNF. There was a slight increase in serum BDNF levels after stroke. There was no overall difference between stroke patients and controls in the frequency of CD4+ and CD8+ BDNF+ cells, although a subgroup of stroke patients showed high frequencies of these cells. However, there was an increase in the percentage of BDNF+ Treg cells in the CD4+ population in stroke patients compared to controls. Patients with high percentages of CD4+ BDNF+ Treg cells had a better outcome at 6 months than those with lower levels. These groups did not differ in age, gender or initial stroke severity. Enhancement of BDNF production after stroke could be a useful means of improving neuroprotection and recovery after stroke.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/j.jneuroim.2015.06.013</identifier><identifier>PMID: 26298323</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acute ischemic stroke ; Adult ; Aged ; Aged, 80 and over ; Allergy and Immunology ; Antigens, CD - metabolism ; Brain derived neurotrophic factor ; Brain-Derived Neurotrophic Factor - metabolism ; Cells, Cultured ; Enzyme-Linked Immunosorbent Assay ; Female ; Flow Cytometry ; FoxP3 ; Humans ; Male ; Middle Aged ; Neurology ; Neuroprotection ; Protective immunity ; Regulatory T cells ; Severity of Illness Index ; Stroke - blood ; Stroke - immunology ; Stroke outcome ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; Time Factors ; Young Adult</subject><ispartof>Journal of neuroimmunology, 2015-09, Vol.286, p.42-47</ispartof><rights>Elsevier B.V.</rights><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-4d4ddb28b6b2249959b14178c7ffea56b863ec69196b5a87f8b5d9b007a39563</citedby><cites>FETCH-LOGICAL-c456t-4d4ddb28b6b2249959b14178c7ffea56b863ec69196b5a87f8b5d9b007a39563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jneuroim.2015.06.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26298323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chan, Adeline</creatorcontrib><creatorcontrib>Yan, Jun</creatorcontrib><creatorcontrib>Csurhes, Peter</creatorcontrib><creatorcontrib>Greer, Judith</creatorcontrib><creatorcontrib>McCombe, Pamela</creatorcontrib><title>Circulating brain derived neurotrophic factor (BDNF) and frequency of BDNF positive T cells in peripheral blood in human ischemic stroke: Effect on outcome</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>Abstract The aim of this study was to measure the levels of circulating BDNF and the frequency of BDNF-producing T cells after acute ischaemic stroke. Serum BDNF levels were measured by ELISA. Flow cytometry was used to enumerate peripheral blood leukocytes that were labelled with antibodies against markers of T cells, T regulatory cells (Tregs), and intracellular BDNF. There was a slight increase in serum BDNF levels after stroke. There was no overall difference between stroke patients and controls in the frequency of CD4+ and CD8+ BDNF+ cells, although a subgroup of stroke patients showed high frequencies of these cells. However, there was an increase in the percentage of BDNF+ Treg cells in the CD4+ population in stroke patients compared to controls. Patients with high percentages of CD4+ BDNF+ Treg cells had a better outcome at 6 months than those with lower levels. These groups did not differ in age, gender or initial stroke severity. Enhancement of BDNF production after stroke could be a useful means of improving neuroprotection and recovery after stroke.</description><subject>Acute ischemic stroke</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Allergy and Immunology</subject><subject>Antigens, CD - metabolism</subject><subject>Brain derived neurotrophic factor</subject><subject>Brain-Derived Neurotrophic Factor - metabolism</subject><subject>Cells, Cultured</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>FoxP3</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neurology</subject><subject>Neuroprotection</subject><subject>Protective immunity</subject><subject>Regulatory T cells</subject><subject>Severity of Illness Index</subject><subject>Stroke - blood</subject><subject>Stroke - immunology</subject><subject>Stroke outcome</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>Time Factors</subject><subject>Young Adult</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks9u1DAQxiMEokvhFSofyyHBdmIn4YCApQWkCg7s3fKfCettEgc7qbTPwssyYVsOXOBkafSbbzzzfVl2wWjBKJOvDsVhhCUGPxScMlFQWVBWPso2rKl53lScPc42CIpc1Lw5y56ldKAIllX7NDvjkrdNyctN9nPro116PfvxOzFR-5E4iP4OHPmtP8cw7b0lnbZziOTy_Ycv1y-JHh3pIvxYYLRHEjqylskUkp-xleyIhb5PBMUmFJv2EHVPTB-CW2v7ZdAj8cnuYUDphDNu4TW56jqwMwkjCctswwDPsyed7hO8uH_Ps9311W77Kb_5-vHz9t1Nbish57xylXOGN0Yazqu2Fa1hFasbW6OgFtI0sgQrW9ZKI3RTd40RrjWU1rpshSzPs8uT7BQDbpRmNeDfcAE9QliSYjWvZU0bxv4DpVLUkskSUXlCbQwpRejUFP2g41ExqlYL1UE9WKhWCxWVCi3Exov7GYsZwP1pe_AMgbcnAPAmdx6iStajEeB8xAMqF_y_Z7z5S8L2fvRW97dwhHQISxzx4oqpxBVV39YgrTnC_FBMESt_ATwyxnY</recordid><startdate>20150915</startdate><enddate>20150915</enddate><creator>Chan, Adeline</creator><creator>Yan, Jun</creator><creator>Csurhes, Peter</creator><creator>Greer, Judith</creator><creator>McCombe, Pamela</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope></search><sort><creationdate>20150915</creationdate><title>Circulating brain derived neurotrophic factor (BDNF) and frequency of BDNF positive T cells in peripheral blood in human ischemic stroke: Effect on outcome</title><author>Chan, Adeline ; Yan, Jun ; Csurhes, Peter ; Greer, Judith ; McCombe, Pamela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-4d4ddb28b6b2249959b14178c7ffea56b863ec69196b5a87f8b5d9b007a39563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acute ischemic stroke</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Allergy and Immunology</topic><topic>Antigens, CD - metabolism</topic><topic>Brain derived neurotrophic factor</topic><topic>Brain-Derived Neurotrophic Factor - metabolism</topic><topic>Cells, Cultured</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>FoxP3</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neurology</topic><topic>Neuroprotection</topic><topic>Protective immunity</topic><topic>Regulatory T cells</topic><topic>Severity of Illness Index</topic><topic>Stroke - blood</topic><topic>Stroke - immunology</topic><topic>Stroke outcome</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>Time Factors</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chan, Adeline</creatorcontrib><creatorcontrib>Yan, Jun</creatorcontrib><creatorcontrib>Csurhes, Peter</creatorcontrib><creatorcontrib>Greer, Judith</creatorcontrib><creatorcontrib>McCombe, Pamela</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chan, Adeline</au><au>Yan, Jun</au><au>Csurhes, Peter</au><au>Greer, Judith</au><au>McCombe, Pamela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating brain derived neurotrophic factor (BDNF) and frequency of BDNF positive T cells in peripheral blood in human ischemic stroke: Effect on outcome</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>2015-09-15</date><risdate>2015</risdate><volume>286</volume><spage>42</spage><epage>47</epage><pages>42-47</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><abstract>Abstract The aim of this study was to measure the levels of circulating BDNF and the frequency of BDNF-producing T cells after acute ischaemic stroke. Serum BDNF levels were measured by ELISA. Flow cytometry was used to enumerate peripheral blood leukocytes that were labelled with antibodies against markers of T cells, T regulatory cells (Tregs), and intracellular BDNF. There was a slight increase in serum BDNF levels after stroke. There was no overall difference between stroke patients and controls in the frequency of CD4+ and CD8+ BDNF+ cells, although a subgroup of stroke patients showed high frequencies of these cells. However, there was an increase in the percentage of BDNF+ Treg cells in the CD4+ population in stroke patients compared to controls. Patients with high percentages of CD4+ BDNF+ Treg cells had a better outcome at 6 months than those with lower levels. These groups did not differ in age, gender or initial stroke severity. Enhancement of BDNF production after stroke could be a useful means of improving neuroprotection and recovery after stroke.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26298323</pmid><doi>10.1016/j.jneuroim.2015.06.013</doi><tpages>6</tpages></addata></record> |
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| subjects | Acute ischemic stroke Adult Aged Aged, 80 and over Allergy and Immunology Antigens, CD - metabolism Brain derived neurotrophic factor Brain-Derived Neurotrophic Factor - metabolism Cells, Cultured Enzyme-Linked Immunosorbent Assay Female Flow Cytometry FoxP3 Humans Male Middle Aged Neurology Neuroprotection Protective immunity Regulatory T cells Severity of Illness Index Stroke - blood Stroke - immunology Stroke outcome T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism Time Factors Young Adult |
| title | Circulating brain derived neurotrophic factor (BDNF) and frequency of BDNF positive T cells in peripheral blood in human ischemic stroke: Effect on outcome |
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