Preparation of a cyanine-based fluorescent probe for highly selective detection of glutathione and its use in living cells and tissues of mice

Changes in the levels of cellular thiols (e.g., glutathione) are linked to many diseases. This protocol is for the synthesis of CPDSA, a fluorescent turn-on glutathione probe with near-infrared emission. In-cell and in vivo assays are also described. Glutathione (GSH) is a major endogenous antioxida...

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