Suppression and overexpression of ubiquitin extension protein S27a affects cell proliferation and in vitro regeneration in Nicotiana benthamiana
Ubiquitin is a highly conserved 76-amino-acid protein found in all eukaryotic cells. Ubiquitin's expression is encoded and expressed as multimeric head-to-tail repeats (polyubiquitins) that are post-translationally cleaved into monomers, or fused with ribosomal proteins S27a and L40. S27a is hi...
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Veröffentlicht in: | Plant science (Limerick) 2009-04, Vol.176 (4), p.566-574 |
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creator | Hanania, Uri Velcheva, Margarita Sahar, Nachman Flaishman, Moshe Or, Etti Dgani, Oded Perl, Avihai |
description | Ubiquitin is a highly conserved 76-amino-acid protein found in all eukaryotic cells. Ubiquitin's expression is encoded and expressed as multimeric head-to-tail repeats (polyubiquitins) that are post-translationally cleaved into monomers, or fused with ribosomal proteins S27a and L40. S27a is highly expressed in meristematic tissues, pollen and ovules and its ubiquitin moiety is thought to act as a chaperone in ribosome biogenesis prior to cleavage. This study suggests that the ribosomal protein S27a plays a critical role in the allocation of meristematic cells that differentiate into lateral structures such as leaves and flowers. S27a was also found to regulate floral meristem development, possibly through the control of cell proliferation as well as cell identity. Overexpression of S27a was correlated with increased proliferation of undifferentiated cells and arrest of morphologically “normal” shoot and leaf development. The ubiquitin moiety did not affect the localization of S27a, but it did affect its protein level: expression of S27a without the ubiquitin moiety caused a severe reduction in S27a protein level. |
doi_str_mv | 10.1016/j.plantsci.2009.01.019 |
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Ubiquitin's expression is encoded and expressed as multimeric head-to-tail repeats (polyubiquitins) that are post-translationally cleaved into monomers, or fused with ribosomal proteins S27a and L40. S27a is highly expressed in meristematic tissues, pollen and ovules and its ubiquitin moiety is thought to act as a chaperone in ribosome biogenesis prior to cleavage. This study suggests that the ribosomal protein S27a plays a critical role in the allocation of meristematic cells that differentiate into lateral structures such as leaves and flowers. S27a was also found to regulate floral meristem development, possibly through the control of cell proliferation as well as cell identity. Overexpression of S27a was correlated with increased proliferation of undifferentiated cells and arrest of morphologically “normal” shoot and leaf development. The ubiquitin moiety did not affect the localization of S27a, but it did affect its protein level: expression of S27a without the ubiquitin moiety caused a severe reduction in S27a protein level.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/j.plantsci.2009.01.019</identifier><identifier>PMID: 26493147</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; apical meristems ; Biological and medical sciences ; cell differentiation ; cell proliferation ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; General agronomy. Plant production ; N. benthamiana ; Nicotiana benthamiana ; Phytopathology. Animal pests. Plant and forest protection ; Plant physiology and development ; plant proteins ; plant regeneration ; protein content ; ribosomal proteins ; Shoot apical meristem ; shoot meristems ; Tissue cultures, protoplasts ; Tobacco rattle virus ; ubiquitin ; Ubiquitin extension S27a ; Vegetative propagation. Micropropagation ; Vegetative propagation. Sowing and planting. Harvesting</subject><ispartof>Plant science (Limerick), 2009-04, Vol.176 (4), p.566-574</ispartof><rights>2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright © 2009. 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Ubiquitin's expression is encoded and expressed as multimeric head-to-tail repeats (polyubiquitins) that are post-translationally cleaved into monomers, or fused with ribosomal proteins S27a and L40. S27a is highly expressed in meristematic tissues, pollen and ovules and its ubiquitin moiety is thought to act as a chaperone in ribosome biogenesis prior to cleavage. This study suggests that the ribosomal protein S27a plays a critical role in the allocation of meristematic cells that differentiate into lateral structures such as leaves and flowers. S27a was also found to regulate floral meristem development, possibly through the control of cell proliferation as well as cell identity. Overexpression of S27a was correlated with increased proliferation of undifferentiated cells and arrest of morphologically “normal” shoot and leaf development. The ubiquitin moiety did not affect the localization of S27a, but it did affect its protein level: expression of S27a without the ubiquitin moiety caused a severe reduction in S27a protein level.</description><subject>Agronomy. Soil science and plant productions</subject><subject>apical meristems</subject><subject>Biological and medical sciences</subject><subject>cell differentiation</subject><subject>cell proliferation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>General agronomy. Plant production</subject><subject>N. benthamiana</subject><subject>Nicotiana benthamiana</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>plant regeneration</subject><subject>protein content</subject><subject>ribosomal proteins</subject><subject>Shoot apical meristem</subject><subject>shoot meristems</subject><subject>Tissue cultures, protoplasts</subject><subject>Tobacco rattle virus</subject><subject>ubiquitin</subject><subject>Ubiquitin extension S27a</subject><subject>Vegetative propagation. Micropropagation</subject><subject>Vegetative propagation. Sowing and planting. Harvesting</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAQxy0EokvhK5RckLhk8SOJ7Ruo4iVVcFh6tibOpHiVtbe2syrfgo-Mw-6WI9JIHv3nNw_PEHLF6JpR1r3brvcT-JysW3NK9ZqyYvoJWTElRc15q5-SVQFVrZuWX5AXKW0ppbxt5XNywbtGC9bIFfm9mff7iCm54CvwQxUOGPHhUQpjNffufnbZ-QofMvq_8j6GjEXZcAkVjCPanCqL07REJjdihHyuWLCDyzFUEe_QnyNF_eZsyA48VD36_BN2i_-SPBthSvjq9F6S208ff1x_qW--f_56_eGmtg3nudYAoGk_tLJvcEBlB0GpAgEglcC-Z6Kz2vZNbztUgxQNKz5K3TDFW0WVuCRvj3XLwPczpmx2Li0_AI9hToZJLru26VpR0O6I2hhSijiafXQ7iL8Mo2a5htma8zXMcg1DWTFdEq9OPeZ-h8Nj2nn9BXhzAiBZmMYI3rr0j2O8Y0rRwr0-ciMEA3exMLcbTpkozSlT3dLq_ZHAsrODw2jKMOgtDi6W45ghuP9N-wet17lN</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Hanania, Uri</creator><creator>Velcheva, Margarita</creator><creator>Sahar, Nachman</creator><creator>Flaishman, Moshe</creator><creator>Or, Etti</creator><creator>Dgani, Oded</creator><creator>Perl, Avihai</creator><general>Elsevier Ireland Ltd</general><general>[Ireland]: Elsevier Science Ireland Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Suppression and overexpression of ubiquitin extension protein S27a affects cell proliferation and in vitro regeneration in Nicotiana benthamiana</title><author>Hanania, Uri ; Velcheva, Margarita ; Sahar, Nachman ; Flaishman, Moshe ; Or, Etti ; Dgani, Oded ; Perl, Avihai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-9aaa90bd57b4ede8cd3008a3aa783ebb136c9cb4bc6e8d7341b4be79418258083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>apical meristems</topic><topic>Biological and medical sciences</topic><topic>cell differentiation</topic><topic>cell proliferation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>General agronomy. Plant production</topic><topic>N. benthamiana</topic><topic>Nicotiana benthamiana</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>plant regeneration</topic><topic>protein content</topic><topic>ribosomal proteins</topic><topic>Shoot apical meristem</topic><topic>shoot meristems</topic><topic>Tissue cultures, protoplasts</topic><topic>Tobacco rattle virus</topic><topic>ubiquitin</topic><topic>Ubiquitin extension S27a</topic><topic>Vegetative propagation. Micropropagation</topic><topic>Vegetative propagation. Sowing and planting. Harvesting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hanania, Uri</creatorcontrib><creatorcontrib>Velcheva, Margarita</creatorcontrib><creatorcontrib>Sahar, Nachman</creatorcontrib><creatorcontrib>Flaishman, Moshe</creatorcontrib><creatorcontrib>Or, Etti</creatorcontrib><creatorcontrib>Dgani, Oded</creatorcontrib><creatorcontrib>Perl, Avihai</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hanania, Uri</au><au>Velcheva, Margarita</au><au>Sahar, Nachman</au><au>Flaishman, Moshe</au><au>Or, Etti</au><au>Dgani, Oded</au><au>Perl, Avihai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppression and overexpression of ubiquitin extension protein S27a affects cell proliferation and in vitro regeneration in Nicotiana benthamiana</atitle><jtitle>Plant science (Limerick)</jtitle><addtitle>Plant Sci</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>176</volume><issue>4</issue><spage>566</spage><epage>574</epage><pages>566-574</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>Ubiquitin is a highly conserved 76-amino-acid protein found in all eukaryotic cells. Ubiquitin's expression is encoded and expressed as multimeric head-to-tail repeats (polyubiquitins) that are post-translationally cleaved into monomers, or fused with ribosomal proteins S27a and L40. S27a is highly expressed in meristematic tissues, pollen and ovules and its ubiquitin moiety is thought to act as a chaperone in ribosome biogenesis prior to cleavage. This study suggests that the ribosomal protein S27a plays a critical role in the allocation of meristematic cells that differentiate into lateral structures such as leaves and flowers. S27a was also found to regulate floral meristem development, possibly through the control of cell proliferation as well as cell identity. Overexpression of S27a was correlated with increased proliferation of undifferentiated cells and arrest of morphologically “normal” shoot and leaf development. The ubiquitin moiety did not affect the localization of S27a, but it did affect its protein level: expression of S27a without the ubiquitin moiety caused a severe reduction in S27a protein level.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>26493147</pmid><doi>10.1016/j.plantsci.2009.01.019</doi><tpages>9</tpages></addata></record> |
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subjects | Agronomy. Soil science and plant productions apical meristems Biological and medical sciences cell differentiation cell proliferation Fundamental and applied biological sciences. Psychology gene expression regulation General agronomy. Plant production N. benthamiana Nicotiana benthamiana Phytopathology. Animal pests. Plant and forest protection Plant physiology and development plant proteins plant regeneration protein content ribosomal proteins Shoot apical meristem shoot meristems Tissue cultures, protoplasts Tobacco rattle virus ubiquitin Ubiquitin extension S27a Vegetative propagation. Micropropagation Vegetative propagation. Sowing and planting. Harvesting |
title | Suppression and overexpression of ubiquitin extension protein S27a affects cell proliferation and in vitro regeneration in Nicotiana benthamiana |
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