Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Captive Wildlife at Zhengzhou Zoo, China
Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are common gastrointestinal protists in humans and animals. Two hundred and three fecal specimens from 80 wildlife species were collected in Zhengzhou Zoo and their genomic DNA extracted. Three intestinal pathogens were characteri...
Gespeichert in:
| Veröffentlicht in: | The Journal of eukaryotic microbiology 2015-11, Vol.62 (6), p.833-839 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 839 |
|---|---|
| container_issue | 6 |
| container_start_page | 833 |
| container_title | The Journal of eukaryotic microbiology |
| container_volume | 62 |
| creator | Li, Junqiang Qi, Meng Chang, Yankai Wang, Rongjun Li, Tongyi Dong, Haiju Zhang, Longxian |
| description | Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are common gastrointestinal protists in humans and animals. Two hundred and three fecal specimens from 80 wildlife species were collected in Zhengzhou Zoo and their genomic DNA extracted. Three intestinal pathogens were characterized with a DNA sequence analysis of different loci. Cryptosporidium felis, C. baileyi, and avian genotype III were identified in three specimens (1.5%), the manul, red‐crowned crane, and cockatiel, respectively. Giardia duodenalis was also found in five specimens (2.5%) firstly: assemblage B in a white‐cheeked gibbon and beaver, and assemblage F in a Chinese leopard and two Siberian tigers, respectively. Thirteen genotypes of E. bieneusi (seven previously reported genotypes and six new genotypes) were detected in 32 specimens (15.8%), of which most were reported for the first time. A phylogenetic analysis of E. bieneusi showed that five genotypes (three known and two new) clustered in group 1; three known genotypes clustered in group 2; one known genotype clustered in group 4; and the remaining four genotypes clustered in a new group. In conclusion, zoonotic Cryptosporidium spp., G. duodenalis, and E. bieneusi are maintained in wildlife and transmitted between them. Zoonotic disease outbreaks of these infectious agents possibly originate in wildlife reservoirs. |
| doi_str_mv | 10.1111/jeu.12269 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1727438544</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1727438544</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4539-22417943b5ef33ad30e399899759d69a5bcc2979d85d8f803c8bfad44746bb2a3</originalsourceid><addsrcrecordid>eNp1kc1u1DAUhSMEoj-w4AXASypNpv6N4yUKwxRUQKgdKnVj3cROxyUTBzsBZl6CV8YwbXfczb2L75wrnZNlLwiekzSnt3aaE0oL9Sg7JELgXHJcPk43LopcUMYPsqMYbzEmBSXkaXZAC1ZyUdLD7PdH39lm6iCgag0BmtEGt4PR-R75FlVhO4w-Dj4446YNisMwn6Glg2AcIDN5Y3voXJwh6A1a9Entm-3odz7pa2d7O0WHXI8qGEb3w6Ir15nOtRbBiK7Xtr_Zrf2Err2fpf-uh2fZkxa6aJ_f7eNs9W5xWZ3l55-X76s353nDBVM5pZxIxVktbMsYGIYtU6pUSgplCgWibhqqpDKlMGVbYtaUdQuGc8mLuqbAjrPXe98h-O-TjaPeuNjYroPe-ilqIqnkrBScJ_RkjzbBxxhsq4fgNhC2mmD9N3-d8tf_8k_syzvbqd5Y80DeB56A0z3w03V2-38n_WGxurfM9woXR_vrQQHhmy4kk0JffVpq_PXiy5kkl_pt4l_t-Ra8hpvgol5d0FR9qp9SxgT7A44IqVQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1727438544</pqid></control><display><type>article</type><title>Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Captive Wildlife at Zhengzhou Zoo, China</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Li, Junqiang ; Qi, Meng ; Chang, Yankai ; Wang, Rongjun ; Li, Tongyi ; Dong, Haiju ; Zhang, Longxian</creator><creatorcontrib>Li, Junqiang ; Qi, Meng ; Chang, Yankai ; Wang, Rongjun ; Li, Tongyi ; Dong, Haiju ; Zhang, Longxian</creatorcontrib><description>Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are common gastrointestinal protists in humans and animals. Two hundred and three fecal specimens from 80 wildlife species were collected in Zhengzhou Zoo and their genomic DNA extracted. Three intestinal pathogens were characterized with a DNA sequence analysis of different loci. Cryptosporidium felis, C. baileyi, and avian genotype III were identified in three specimens (1.5%), the manul, red‐crowned crane, and cockatiel, respectively. Giardia duodenalis was also found in five specimens (2.5%) firstly: assemblage B in a white‐cheeked gibbon and beaver, and assemblage F in a Chinese leopard and two Siberian tigers, respectively. Thirteen genotypes of E. bieneusi (seven previously reported genotypes and six new genotypes) were detected in 32 specimens (15.8%), of which most were reported for the first time. A phylogenetic analysis of E. bieneusi showed that five genotypes (three known and two new) clustered in group 1; three known genotypes clustered in group 2; one known genotype clustered in group 4; and the remaining four genotypes clustered in a new group. In conclusion, zoonotic Cryptosporidium spp., G. duodenalis, and E. bieneusi are maintained in wildlife and transmitted between them. Zoonotic disease outbreaks of these infectious agents possibly originate in wildlife reservoirs.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/jeu.12269</identifier><identifier>PMID: 26384582</identifier><language>eng</language><publisher>United States: Society of Protozoologists</publisher><subject>Animals ; Animals, Zoo - parasitology ; birds ; captive animals ; China ; Cryptosporidiosis - genetics ; Cryptosporidiosis - parasitology ; Cryptosporidium ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; disease outbreaks ; DNA ; DNA, Protozoan - genetics ; Enterocytozoon - genetics ; Enterocytozoon - isolation & purification ; feces ; Feces - parasitology ; Female ; gastrointestinal system ; Genotype ; Genotyping ; Giardia lamblia ; Giardia lamblia - genetics ; Giardia lamblia - isolation & purification ; Giardiasis - genetics ; Giardiasis - parasitology ; Giardiasis - veterinary ; Grus japonensis ; humans ; loci ; Male ; Microsporidiosis - genetics ; Microsporidiosis - parasitology ; Microsporidiosis - veterinary ; nucleotide sequences ; Panthera pardus ; Panthera tigris altaica ; pathogens ; Phylogeny ; sequence analysis ; Sequence Analysis, DNA ; wildlife ; zoonoses ; Zoonoses - parasitology ; zoos</subject><ispartof>The Journal of eukaryotic microbiology, 2015-11, Vol.62 (6), p.833-839</ispartof><rights>2015 The Author(s) Journal of Eukaryotic Microbiology © 2015 International Society of Protistologists</rights><rights>2015 The Author(s) Journal of Eukaryotic Microbiology © 2015 International Society of Protistologists.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4539-22417943b5ef33ad30e399899759d69a5bcc2979d85d8f803c8bfad44746bb2a3</citedby><cites>FETCH-LOGICAL-c4539-22417943b5ef33ad30e399899759d69a5bcc2979d85d8f803c8bfad44746bb2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjeu.12269$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjeu.12269$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26384582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Junqiang</creatorcontrib><creatorcontrib>Qi, Meng</creatorcontrib><creatorcontrib>Chang, Yankai</creatorcontrib><creatorcontrib>Wang, Rongjun</creatorcontrib><creatorcontrib>Li, Tongyi</creatorcontrib><creatorcontrib>Dong, Haiju</creatorcontrib><creatorcontrib>Zhang, Longxian</creatorcontrib><title>Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Captive Wildlife at Zhengzhou Zoo, China</title><title>The Journal of eukaryotic microbiology</title><addtitle>J. Eukaryot. Microbiol</addtitle><description>Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are common gastrointestinal protists in humans and animals. Two hundred and three fecal specimens from 80 wildlife species were collected in Zhengzhou Zoo and their genomic DNA extracted. Three intestinal pathogens were characterized with a DNA sequence analysis of different loci. Cryptosporidium felis, C. baileyi, and avian genotype III were identified in three specimens (1.5%), the manul, red‐crowned crane, and cockatiel, respectively. Giardia duodenalis was also found in five specimens (2.5%) firstly: assemblage B in a white‐cheeked gibbon and beaver, and assemblage F in a Chinese leopard and two Siberian tigers, respectively. Thirteen genotypes of E. bieneusi (seven previously reported genotypes and six new genotypes) were detected in 32 specimens (15.8%), of which most were reported for the first time. A phylogenetic analysis of E. bieneusi showed that five genotypes (three known and two new) clustered in group 1; three known genotypes clustered in group 2; one known genotype clustered in group 4; and the remaining four genotypes clustered in a new group. In conclusion, zoonotic Cryptosporidium spp., G. duodenalis, and E. bieneusi are maintained in wildlife and transmitted between them. Zoonotic disease outbreaks of these infectious agents possibly originate in wildlife reservoirs.</description><subject>Animals</subject><subject>Animals, Zoo - parasitology</subject><subject>birds</subject><subject>captive animals</subject><subject>China</subject><subject>Cryptosporidiosis - genetics</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>disease outbreaks</subject><subject>DNA</subject><subject>DNA, Protozoan - genetics</subject><subject>Enterocytozoon - genetics</subject><subject>Enterocytozoon - isolation & purification</subject><subject>feces</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>gastrointestinal system</subject><subject>Genotype</subject><subject>Genotyping</subject><subject>Giardia lamblia</subject><subject>Giardia lamblia - genetics</subject><subject>Giardia lamblia - isolation & purification</subject><subject>Giardiasis - genetics</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - veterinary</subject><subject>Grus japonensis</subject><subject>humans</subject><subject>loci</subject><subject>Male</subject><subject>Microsporidiosis - genetics</subject><subject>Microsporidiosis - parasitology</subject><subject>Microsporidiosis - veterinary</subject><subject>nucleotide sequences</subject><subject>Panthera pardus</subject><subject>Panthera tigris altaica</subject><subject>pathogens</subject><subject>Phylogeny</subject><subject>sequence analysis</subject><subject>Sequence Analysis, DNA</subject><subject>wildlife</subject><subject>zoonoses</subject><subject>Zoonoses - parasitology</subject><subject>zoos</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAUhSMEoj-w4AXASypNpv6N4yUKwxRUQKgdKnVj3cROxyUTBzsBZl6CV8YwbXfczb2L75wrnZNlLwiekzSnt3aaE0oL9Sg7JELgXHJcPk43LopcUMYPsqMYbzEmBSXkaXZAC1ZyUdLD7PdH39lm6iCgag0BmtEGt4PR-R75FlVhO4w-Dj4446YNisMwn6Glg2AcIDN5Y3voXJwh6A1a9Entm-3odz7pa2d7O0WHXI8qGEb3w6Ir15nOtRbBiK7Xtr_Zrf2Err2fpf-uh2fZkxa6aJ_f7eNs9W5xWZ3l55-X76s353nDBVM5pZxIxVktbMsYGIYtU6pUSgplCgWibhqqpDKlMGVbYtaUdQuGc8mLuqbAjrPXe98h-O-TjaPeuNjYroPe-ilqIqnkrBScJ_RkjzbBxxhsq4fgNhC2mmD9N3-d8tf_8k_syzvbqd5Y80DeB56A0z3w03V2-38n_WGxurfM9woXR_vrQQHhmy4kk0JffVpq_PXiy5kkl_pt4l_t-Ra8hpvgol5d0FR9qp9SxgT7A44IqVQ</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>Li, Junqiang</creator><creator>Qi, Meng</creator><creator>Chang, Yankai</creator><creator>Wang, Rongjun</creator><creator>Li, Tongyi</creator><creator>Dong, Haiju</creator><creator>Zhang, Longxian</creator><general>Society of Protozoologists</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201511</creationdate><title>Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Captive Wildlife at Zhengzhou Zoo, China</title><author>Li, Junqiang ; Qi, Meng ; Chang, Yankai ; Wang, Rongjun ; Li, Tongyi ; Dong, Haiju ; Zhang, Longxian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4539-22417943b5ef33ad30e399899759d69a5bcc2979d85d8f803c8bfad44746bb2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Animals, Zoo - parasitology</topic><topic>birds</topic><topic>captive animals</topic><topic>China</topic><topic>Cryptosporidiosis - genetics</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>disease outbreaks</topic><topic>DNA</topic><topic>DNA, Protozoan - genetics</topic><topic>Enterocytozoon - genetics</topic><topic>Enterocytozoon - isolation & purification</topic><topic>feces</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>gastrointestinal system</topic><topic>Genotype</topic><topic>Genotyping</topic><topic>Giardia lamblia</topic><topic>Giardia lamblia - genetics</topic><topic>Giardia lamblia - isolation & purification</topic><topic>Giardiasis - genetics</topic><topic>Giardiasis - parasitology</topic><topic>Giardiasis - veterinary</topic><topic>Grus japonensis</topic><topic>humans</topic><topic>loci</topic><topic>Male</topic><topic>Microsporidiosis - genetics</topic><topic>Microsporidiosis - parasitology</topic><topic>Microsporidiosis - veterinary</topic><topic>nucleotide sequences</topic><topic>Panthera pardus</topic><topic>Panthera tigris altaica</topic><topic>pathogens</topic><topic>Phylogeny</topic><topic>sequence analysis</topic><topic>Sequence Analysis, DNA</topic><topic>wildlife</topic><topic>zoonoses</topic><topic>Zoonoses - parasitology</topic><topic>zoos</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Junqiang</creatorcontrib><creatorcontrib>Qi, Meng</creatorcontrib><creatorcontrib>Chang, Yankai</creatorcontrib><creatorcontrib>Wang, Rongjun</creatorcontrib><creatorcontrib>Li, Tongyi</creatorcontrib><creatorcontrib>Dong, Haiju</creatorcontrib><creatorcontrib>Zhang, Longxian</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Junqiang</au><au>Qi, Meng</au><au>Chang, Yankai</au><au>Wang, Rongjun</au><au>Li, Tongyi</au><au>Dong, Haiju</au><au>Zhang, Longxian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Captive Wildlife at Zhengzhou Zoo, China</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J. Eukaryot. Microbiol</addtitle><date>2015-11</date><risdate>2015</risdate><volume>62</volume><issue>6</issue><spage>833</spage><epage>839</epage><pages>833-839</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><abstract>Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are common gastrointestinal protists in humans and animals. Two hundred and three fecal specimens from 80 wildlife species were collected in Zhengzhou Zoo and their genomic DNA extracted. Three intestinal pathogens were characterized with a DNA sequence analysis of different loci. Cryptosporidium felis, C. baileyi, and avian genotype III were identified in three specimens (1.5%), the manul, red‐crowned crane, and cockatiel, respectively. Giardia duodenalis was also found in five specimens (2.5%) firstly: assemblage B in a white‐cheeked gibbon and beaver, and assemblage F in a Chinese leopard and two Siberian tigers, respectively. Thirteen genotypes of E. bieneusi (seven previously reported genotypes and six new genotypes) were detected in 32 specimens (15.8%), of which most were reported for the first time. A phylogenetic analysis of E. bieneusi showed that five genotypes (three known and two new) clustered in group 1; three known genotypes clustered in group 2; one known genotype clustered in group 4; and the remaining four genotypes clustered in a new group. In conclusion, zoonotic Cryptosporidium spp., G. duodenalis, and E. bieneusi are maintained in wildlife and transmitted between them. Zoonotic disease outbreaks of these infectious agents possibly originate in wildlife reservoirs.</abstract><cop>United States</cop><pub>Society of Protozoologists</pub><pmid>26384582</pmid><doi>10.1111/jeu.12269</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1066-5234 |
| ispartof | The Journal of eukaryotic microbiology, 2015-11, Vol.62 (6), p.833-839 |
| issn | 1066-5234 1550-7408 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1727438544 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Animals, Zoo - parasitology birds captive animals China Cryptosporidiosis - genetics Cryptosporidiosis - parasitology Cryptosporidium Cryptosporidium - genetics Cryptosporidium - isolation & purification disease outbreaks DNA DNA, Protozoan - genetics Enterocytozoon - genetics Enterocytozoon - isolation & purification feces Feces - parasitology Female gastrointestinal system Genotype Genotyping Giardia lamblia Giardia lamblia - genetics Giardia lamblia - isolation & purification Giardiasis - genetics Giardiasis - parasitology Giardiasis - veterinary Grus japonensis humans loci Male Microsporidiosis - genetics Microsporidiosis - parasitology Microsporidiosis - veterinary nucleotide sequences Panthera pardus Panthera tigris altaica pathogens Phylogeny sequence analysis Sequence Analysis, DNA wildlife zoonoses Zoonoses - parasitology zoos |
| title | Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Captive Wildlife at Zhengzhou Zoo, China |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T18%3A36%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Characterization%20of%20Cryptosporidium%20spp.,%20Giardia%20duodenalis,%20and%20Enterocytozoon%20bieneusi%20in%20Captive%20Wildlife%20at%20Zhengzhou%20Zoo,%20China&rft.jtitle=The%20Journal%20of%20eukaryotic%20microbiology&rft.au=Li,%20Junqiang&rft.date=2015-11&rft.volume=62&rft.issue=6&rft.spage=833&rft.epage=839&rft.pages=833-839&rft.issn=1066-5234&rft.eissn=1550-7408&rft_id=info:doi/10.1111/jeu.12269&rft_dat=%3Cproquest_cross%3E1727438544%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1727438544&rft_id=info:pmid/26384582&rfr_iscdi=true |