An erythromycin resistance cassette and mini-transposon for constructing transcriptional fusions to cat
A new cassette (Er-Cm cassette) and mini-transposon (mTn) (Tn MaxErCm) based on the previously described mTn, Tn Max2 [Haas et al., Gene 130, 23–31.], have been constructed. The cassette and mTn make use of an erythromycin resistance (Er R) marker encoded by ermC′. Both the Er-Cm cassette and Tn Max...
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| Veröffentlicht in: | Gene 1999-03, Vol.229 (1), p.59-65 |
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| creator | Baron, G.S Nano, F.E |
| description | A new cassette (Er-Cm cassette) and mini-transposon (mTn) (Tn
MaxErCm) based on the previously described mTn, Tn
Max2 [Haas et al., Gene 130, 23–31.], have been constructed. The cassette and mTn make use of an erythromycin resistance (Er
R) marker encoded by
ermC′. Both the Er-Cm cassette and Tn
MaxErCm also carry a promoterless
cat gene to allow the construction of transcriptional fusions and the measurement of transcriptional activity by assaying for chloramphenicol acetyltransferase. We show the function of these genetic elements by analyzing the regulation of expression of the
mglA gene of
Francisella novicida and by using Tn
MaxErCm to probe for promoter activity within an
F. novicida recombinant clone. The reporter cassette and mTn described here further expand the family of Tn
Max transposons and facilitate the study of gene expression in organisms where direct Tn mutagenesis methods are unavailable. |
| doi_str_mv | 10.1016/S0378-1119(99)00032-3 |
| format | Article |
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MaxErCm) based on the previously described mTn, Tn
Max2 [Haas et al., Gene 130, 23–31.], have been constructed. The cassette and mTn make use of an erythromycin resistance (Er
R) marker encoded by
ermC′. Both the Er-Cm cassette and Tn
MaxErCm also carry a promoterless
cat gene to allow the construction of transcriptional fusions and the measurement of transcriptional activity by assaying for chloramphenicol acetyltransferase. We show the function of these genetic elements by analyzing the regulation of expression of the
mglA gene of
Francisella novicida and by using Tn
MaxErCm to probe for promoter activity within an
F. novicida recombinant clone. The reporter cassette and mTn described here further expand the family of Tn
Max transposons and facilitate the study of gene expression in organisms where direct Tn mutagenesis methods are unavailable.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(99)00032-3</identifier><identifier>PMID: 10095104</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacterial Proteins - genetics ; Chloramphenicol O-Acetyltransferase - genetics ; DNA Transposable Elements - genetics ; DNA, Bacterial - genetics ; Drug Resistance - genetics ; ermC ; Erythromycin - pharmacology ; F. novicida ; Francisella - genetics ; Francisella novicida ; Gene Expression Regulation, Bacterial - genetics ; Genes, Reporter - genetics ; Genetic Markers - genetics ; mglA ; Mutagenesis, Insertional ; orifd ; Plasmids - genetics ; reporter gene ; Tn MaxErCm ; Transcription, Genetic - genetics</subject><ispartof>Gene, 1999-03, Vol.229 (1), p.59-65</ispartof><rights>1999 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-b9235f9c76add0e056376b3658309abe1d02aa5952b124cb252d5c447baedb13</citedby><cites>FETCH-LOGICAL-c392t-b9235f9c76add0e056376b3658309abe1d02aa5952b124cb252d5c447baedb13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(99)00032-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10095104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baron, G.S</creatorcontrib><creatorcontrib>Nano, F.E</creatorcontrib><title>An erythromycin resistance cassette and mini-transposon for constructing transcriptional fusions to cat</title><title>Gene</title><addtitle>Gene</addtitle><description>A new cassette (Er-Cm cassette) and mini-transposon (mTn) (Tn
MaxErCm) based on the previously described mTn, Tn
Max2 [Haas et al., Gene 130, 23–31.], have been constructed. The cassette and mTn make use of an erythromycin resistance (Er
R) marker encoded by
ermC′. Both the Er-Cm cassette and Tn
MaxErCm also carry a promoterless
cat gene to allow the construction of transcriptional fusions and the measurement of transcriptional activity by assaying for chloramphenicol acetyltransferase. We show the function of these genetic elements by analyzing the regulation of expression of the
mglA gene of
Francisella novicida and by using Tn
MaxErCm to probe for promoter activity within an
F. novicida recombinant clone. The reporter cassette and mTn described here further expand the family of Tn
Max transposons and facilitate the study of gene expression in organisms where direct Tn mutagenesis methods are unavailable.</description><subject>Bacterial Proteins - genetics</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance - genetics</subject><subject>ermC</subject><subject>Erythromycin - pharmacology</subject><subject>F. novicida</subject><subject>Francisella - genetics</subject><subject>Francisella novicida</subject><subject>Gene Expression Regulation, Bacterial - genetics</subject><subject>Genes, Reporter - genetics</subject><subject>Genetic Markers - genetics</subject><subject>mglA</subject><subject>Mutagenesis, Insertional</subject><subject>orifd</subject><subject>Plasmids - genetics</subject><subject>reporter gene</subject><subject>Tn MaxErCm</subject><subject>Transcription, Genetic - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLBDEMgIsouj5-gtKT6GG0j-3M9iQivkDwoPfSaTNa2WnXpiPsv3d2V8SbpyTkS0I-Qo45u-CM15cvTDazinOuz7Q-Z4xJUcktMuGzRldjNdsmk19kj-wjfowQU0rskj3OmFacTSfk7TpSyMvynlO_dCHSDBiw2OiAOosIpQC10dM-xFCVbCMuEqZIu5SpSxFLHlwJ8Y2uey6HRQkp2jntBhwTpCWNi8oh2ensHOHoJx6Q17vb15uH6un5_vHm-qlyUotStVpI1WnX1NZ7BkzVsqlbWauZZNq2wD0T1iqtRMvF1LVCCa_cdNq0FnzL5QE53axd5PQ5ABbTB3Qwn9sIaUDDG9FIruQIqg3ockLM0JlFDr3NS8OZWQk2a8FmZc9obdaCzWru5OfA0Pbg_0xtjI7A1QaA8cuvANmgCzDq9CGDK8an8M-Jb39wjRw</recordid><startdate>19990318</startdate><enddate>19990318</enddate><creator>Baron, G.S</creator><creator>Nano, F.E</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19990318</creationdate><title>An erythromycin resistance cassette and mini-transposon for constructing transcriptional fusions to cat</title><author>Baron, G.S ; Nano, F.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-b9235f9c76add0e056376b3658309abe1d02aa5952b124cb252d5c447baedb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance - genetics</topic><topic>ermC</topic><topic>Erythromycin - pharmacology</topic><topic>F. novicida</topic><topic>Francisella - genetics</topic><topic>Francisella novicida</topic><topic>Gene Expression Regulation, Bacterial - genetics</topic><topic>Genes, Reporter - genetics</topic><topic>Genetic Markers - genetics</topic><topic>mglA</topic><topic>Mutagenesis, Insertional</topic><topic>orifd</topic><topic>Plasmids - genetics</topic><topic>reporter gene</topic><topic>Tn MaxErCm</topic><topic>Transcription, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baron, G.S</creatorcontrib><creatorcontrib>Nano, F.E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baron, G.S</au><au>Nano, F.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An erythromycin resistance cassette and mini-transposon for constructing transcriptional fusions to cat</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1999-03-18</date><risdate>1999</risdate><volume>229</volume><issue>1</issue><spage>59</spage><epage>65</epage><pages>59-65</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A new cassette (Er-Cm cassette) and mini-transposon (mTn) (Tn
MaxErCm) based on the previously described mTn, Tn
Max2 [Haas et al., Gene 130, 23–31.], have been constructed. The cassette and mTn make use of an erythromycin resistance (Er
R) marker encoded by
ermC′. Both the Er-Cm cassette and Tn
MaxErCm also carry a promoterless
cat gene to allow the construction of transcriptional fusions and the measurement of transcriptional activity by assaying for chloramphenicol acetyltransferase. We show the function of these genetic elements by analyzing the regulation of expression of the
mglA gene of
Francisella novicida and by using Tn
MaxErCm to probe for promoter activity within an
F. novicida recombinant clone. The reporter cassette and mTn described here further expand the family of Tn
Max transposons and facilitate the study of gene expression in organisms where direct Tn mutagenesis methods are unavailable.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10095104</pmid><doi>10.1016/S0378-1119(99)00032-3</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1999-03, Vol.229 (1), p.59-65 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17273153 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Bacterial Proteins - genetics Chloramphenicol O-Acetyltransferase - genetics DNA Transposable Elements - genetics DNA, Bacterial - genetics Drug Resistance - genetics ermC Erythromycin - pharmacology F. novicida Francisella - genetics Francisella novicida Gene Expression Regulation, Bacterial - genetics Genes, Reporter - genetics Genetic Markers - genetics mglA Mutagenesis, Insertional orifd Plasmids - genetics reporter gene Tn MaxErCm Transcription, Genetic - genetics |
| title | An erythromycin resistance cassette and mini-transposon for constructing transcriptional fusions to cat |
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