The effect of hydrogen peroxide upon β-adrenoceptor density and function in C6 rat glioma cells
Hydrogen peroxide has been suggested to play an important role in the pathogenesis of Alzheimer fn3 fn3 s disease; [ 3H](−)CGP-12177[(−)-4-(3-t-butylamino-2-hydroxypropoxy)-[5,7, 3H] benzimidazol-2-one]; ICI 118,551, ((±)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(l-methylethyl)amino]-2-butanol...
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| Veröffentlicht in: | Neurochemistry international 1999, Vol.34 (1), p.63-70 |
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| creator | Bergström, D. Jacobsson, S.o. Fowler, C.J. |
| description | Hydrogen peroxide has been suggested to play an important role in the pathogenesis of Alzheimer
fn3
fn3
s disease; [
3H](−)CGP-12177[(−)-4-(3-t-butylamino-2-hydroxypropoxy)-[5,7,
3H] benzimidazol-2-one]; ICI 118,551, ((±)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(l-methylethyl)amino]-2-butanol; PBN,
N-t-butyl-
α-phenylnitrone; Gpp[NH]p, 5′-guanylylimidodiphosphate; LDH, lactate dehydrogenase, FBS, fetal bovine scrum; PBS, phosphate buffered saline.
s disease. In the present study, the effects of hydrogen peroxide upon the functional integrity of β-adrenoceptors have been investigated in C6 glioma cells. Treatment of cells for 24 h with hydrogen peroxide in serum-free medium produced a concentration-dependent cell toxicity, seen both using cell counting and LDH release into medium as end point. There were no large nor consistent changes in either the density of cell surface β
1, or
β
2-adrenoceptors, measured using the hydrophilic ligand [
3H](−)-CGP 12177, nor in either basal, forskolin and isoprenaline-stimulated cAMP responses, following hydrogen peroxide treatment. It is concluded that the decreased adenylyl cyclase activity and responsiveness to G
s stimulation found in post-mortem brain samples from Alzheimers disease autopsy cases is unlikely to be mediated by hydrogen peroxide. |
| doi_str_mv | 10.1016/S0197-0186(98)00070-9 |
| format | Article |
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fn3
fn3
s disease; [
3H](−)CGP-12177[(−)-4-(3-t-butylamino-2-hydroxypropoxy)-[5,7,
3H] benzimidazol-2-one]; ICI 118,551, ((±)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(l-methylethyl)amino]-2-butanol; PBN,
N-t-butyl-
α-phenylnitrone; Gpp[NH]p, 5′-guanylylimidodiphosphate; LDH, lactate dehydrogenase, FBS, fetal bovine scrum; PBS, phosphate buffered saline.
s disease. In the present study, the effects of hydrogen peroxide upon the functional integrity of β-adrenoceptors have been investigated in C6 glioma cells. Treatment of cells for 24 h with hydrogen peroxide in serum-free medium produced a concentration-dependent cell toxicity, seen both using cell counting and LDH release into medium as end point. There were no large nor consistent changes in either the density of cell surface β
1, or
β
2-adrenoceptors, measured using the hydrophilic ligand [
3H](−)-CGP 12177, nor in either basal, forskolin and isoprenaline-stimulated cAMP responses, following hydrogen peroxide treatment. It is concluded that the decreased adenylyl cyclase activity and responsiveness to G
s stimulation found in post-mortem brain samples from Alzheimers disease autopsy cases is unlikely to be mediated by hydrogen peroxide.</description><identifier>ISSN: 0197-0186</identifier><identifier>EISSN: 1872-9754</identifier><identifier>DOI: 10.1016/S0197-0186(98)00070-9</identifier><identifier>PMID: 10100197</identifier><identifier>CODEN: NEUIDS</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>(-)—CGP 12177 ; Adenylyl cyclase ; Adenylyl Cyclases - metabolism ; Adrenergic beta-Agonists - pharmacology ; adrenergic receptors ; Alzheimer's disease ; Animals ; Biological and medical sciences ; Cell Death - drug effects ; Colforsin - pharmacology ; Culture Media, Serum-Free ; Cyclic AMP - metabolism ; Fundamental and applied biological sciences. Psychology ; Glioma - metabolism ; GTP-Binding Protein alpha Subunits, Gs - physiology ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Isolated neuron and nerve. Neuroglia ; Isoproterenol - pharmacology ; L-Lactate Dehydrogenase - metabolism ; Propanolamines - metabolism ; Rats ; Receptors, Adrenergic, beta - drug effects ; Receptors, Adrenergic, beta - physiology ; Tumor Cells, Cultured ; Vertebrates: nervous system and sense organs</subject><ispartof>Neurochemistry international, 1999, Vol.34 (1), p.63-70</ispartof><rights>1999 Elsevier Science Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-157c98a9789e1d27e01b60300ac8c007b6889f15c90aea10abeb695552ec9cc13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0197-0186(98)00070-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,4025,27927,27928,27929,45999</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1698239$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10100197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bergström, D.</creatorcontrib><creatorcontrib>Jacobsson, S.o.</creatorcontrib><creatorcontrib>Fowler, C.J.</creatorcontrib><title>The effect of hydrogen peroxide upon β-adrenoceptor density and function in C6 rat glioma cells</title><title>Neurochemistry international</title><addtitle>Neurochem Int</addtitle><description>Hydrogen peroxide has been suggested to play an important role in the pathogenesis of Alzheimer
fn3
fn3
s disease; [
3H](−)CGP-12177[(−)-4-(3-t-butylamino-2-hydroxypropoxy)-[5,7,
3H] benzimidazol-2-one]; ICI 118,551, ((±)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(l-methylethyl)amino]-2-butanol; PBN,
N-t-butyl-
α-phenylnitrone; Gpp[NH]p, 5′-guanylylimidodiphosphate; LDH, lactate dehydrogenase, FBS, fetal bovine scrum; PBS, phosphate buffered saline.
s disease. In the present study, the effects of hydrogen peroxide upon the functional integrity of β-adrenoceptors have been investigated in C6 glioma cells. Treatment of cells for 24 h with hydrogen peroxide in serum-free medium produced a concentration-dependent cell toxicity, seen both using cell counting and LDH release into medium as end point. There were no large nor consistent changes in either the density of cell surface β
1, or
β
2-adrenoceptors, measured using the hydrophilic ligand [
3H](−)-CGP 12177, nor in either basal, forskolin and isoprenaline-stimulated cAMP responses, following hydrogen peroxide treatment. It is concluded that the decreased adenylyl cyclase activity and responsiveness to G
s stimulation found in post-mortem brain samples from Alzheimers disease autopsy cases is unlikely to be mediated by hydrogen peroxide.</description><subject>(-)—CGP 12177</subject><subject>Adenylyl cyclase</subject><subject>Adenylyl Cyclases - metabolism</subject><subject>Adrenergic beta-Agonists - pharmacology</subject><subject>adrenergic receptors</subject><subject>Alzheimer's disease</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Death - drug effects</subject><subject>Colforsin - pharmacology</subject><subject>Culture Media, Serum-Free</subject><subject>Cyclic AMP - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glioma - metabolism</subject><subject>GTP-Binding Protein alpha Subunits, Gs - physiology</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Isoproterenol - pharmacology</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Propanolamines - metabolism</subject><subject>Rats</subject><subject>Receptors, Adrenergic, beta - drug effects</subject><subject>Receptors, Adrenergic, beta - physiology</subject><subject>Tumor Cells, Cultured</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0197-0186</issn><issn>1872-9754</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhq2qqN0ufYRWPlSoPQTG2TqxTxVaQUGqxIFyNs540rrK2sFOEPtaPAjPRNJdATdOPvibf359w9iZgNcCRPXmMwhdFyBUdanVFQDUUOgDthCqLgtdy-tDtviDHLOTnJ9mSIM8YsdTAsyfC_b1_pE4tS3hwGPLH7cuxQcKvKcUf3hHfOxj4L9-FtYlChGpH2LijkL2w5bb4Hg7Bhz8BPnA1xVPduAPnY8by5G6Lr9kL1rbZTrdv0v25f27-_WH4u7T7cf127sCr0sxFELWqJXVtdIkXFkTiKaCFYBFhVPvplJKt0KiBktWgG2oqbSUsiTUiGK1ZK92uX2K30bKg9n4PDewgeKYjajLSmpVTqDcgZhizola0ye_sWlrBJhZrXlWa2ZvRivzrNboae58v2BsNuT-mdq5nICLPWAz2q5NNqDPf7lq3j7n3Owwmmx895RMRk8Byfk0XcG46P_T5De5EJXm</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Bergström, D.</creator><creator>Jacobsson, S.o.</creator><creator>Fowler, C.J.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>1999</creationdate><title>The effect of hydrogen peroxide upon β-adrenoceptor density and function in C6 rat glioma cells</title><author>Bergström, D. ; Jacobsson, S.o. ; Fowler, C.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-157c98a9789e1d27e01b60300ac8c007b6889f15c90aea10abeb695552ec9cc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>(-)—CGP 12177</topic><topic>Adenylyl cyclase</topic><topic>Adenylyl Cyclases - metabolism</topic><topic>Adrenergic beta-Agonists - pharmacology</topic><topic>adrenergic receptors</topic><topic>Alzheimer's disease</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Death - drug effects</topic><topic>Colforsin - pharmacology</topic><topic>Culture Media, Serum-Free</topic><topic>Cyclic AMP - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glioma - metabolism</topic><topic>GTP-Binding Protein alpha Subunits, Gs - physiology</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Isoproterenol - pharmacology</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Propanolamines - metabolism</topic><topic>Rats</topic><topic>Receptors, Adrenergic, beta - drug effects</topic><topic>Receptors, Adrenergic, beta - physiology</topic><topic>Tumor Cells, Cultured</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bergström, D.</creatorcontrib><creatorcontrib>Jacobsson, S.o.</creatorcontrib><creatorcontrib>Fowler, C.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Neurochemistry international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bergström, D.</au><au>Jacobsson, S.o.</au><au>Fowler, C.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of hydrogen peroxide upon β-adrenoceptor density and function in C6 rat glioma cells</atitle><jtitle>Neurochemistry international</jtitle><addtitle>Neurochem Int</addtitle><date>1999</date><risdate>1999</risdate><volume>34</volume><issue>1</issue><spage>63</spage><epage>70</epage><pages>63-70</pages><issn>0197-0186</issn><eissn>1872-9754</eissn><coden>NEUIDS</coden><abstract>Hydrogen peroxide has been suggested to play an important role in the pathogenesis of Alzheimer
fn3
fn3
s disease; [
3H](−)CGP-12177[(−)-4-(3-t-butylamino-2-hydroxypropoxy)-[5,7,
3H] benzimidazol-2-one]; ICI 118,551, ((±)-1-[2,3-(dihydro-7-methyl-1H-inden-4-yl)oxy]-3-[(l-methylethyl)amino]-2-butanol; PBN,
N-t-butyl-
α-phenylnitrone; Gpp[NH]p, 5′-guanylylimidodiphosphate; LDH, lactate dehydrogenase, FBS, fetal bovine scrum; PBS, phosphate buffered saline.
s disease. In the present study, the effects of hydrogen peroxide upon the functional integrity of β-adrenoceptors have been investigated in C6 glioma cells. Treatment of cells for 24 h with hydrogen peroxide in serum-free medium produced a concentration-dependent cell toxicity, seen both using cell counting and LDH release into medium as end point. There were no large nor consistent changes in either the density of cell surface β
1, or
β
2-adrenoceptors, measured using the hydrophilic ligand [
3H](−)-CGP 12177, nor in either basal, forskolin and isoprenaline-stimulated cAMP responses, following hydrogen peroxide treatment. It is concluded that the decreased adenylyl cyclase activity and responsiveness to G
s stimulation found in post-mortem brain samples from Alzheimers disease autopsy cases is unlikely to be mediated by hydrogen peroxide.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10100197</pmid><doi>10.1016/S0197-0186(98)00070-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Neurochemistry international, 1999, Vol.34 (1), p.63-70 |
| issn | 0197-0186 1872-9754 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17265982 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | (-)—CGP 12177 Adenylyl cyclase Adenylyl Cyclases - metabolism Adrenergic beta-Agonists - pharmacology adrenergic receptors Alzheimer's disease Animals Biological and medical sciences Cell Death - drug effects Colforsin - pharmacology Culture Media, Serum-Free Cyclic AMP - metabolism Fundamental and applied biological sciences. Psychology Glioma - metabolism GTP-Binding Protein alpha Subunits, Gs - physiology Hydrogen peroxide Hydrogen Peroxide - pharmacology Isolated neuron and nerve. Neuroglia Isoproterenol - pharmacology L-Lactate Dehydrogenase - metabolism Propanolamines - metabolism Rats Receptors, Adrenergic, beta - drug effects Receptors, Adrenergic, beta - physiology Tumor Cells, Cultured Vertebrates: nervous system and sense organs |
| title | The effect of hydrogen peroxide upon β-adrenoceptor density and function in C6 rat glioma cells |
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