Substitution at Residue 214 of Human Thymidylate Synthase Alters Nucleotide Binding and Isomerization of Ligand−Protein Complexes

Based on crystal structures of bacterial thymidylate synthases (TS), a glutamine corresponding to residue 214 in human TS (hTS) is located in a region that is postulated to be critical for conformational changes that occur upon ligand binding. Previous steady-state kinetic studies indicated that rep...

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