US3 protein kinase of herpes simplex virus type 2 plays a role in protecting corneal epithelial cells from apoptosis in infected mice
S Asano, T Honda, F Goshima, D Watanabe, Y Miyake, Y Sugiura and Y Nishiyama Research Institute for Disease Mechanism and Control, Department of Ophthalmology, Nagoya University School of Medicine, Japan. To clarify the biological role of US3 protein kinase of herpes simplex virus type 2 (HSV-2) in...
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| Veröffentlicht in: | Journal of general virology 1999-01, Vol.80 (1), p.51-56 |
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| creator | Asano, S Honda, T Goshima, F Watanabe, D Miyake, Y Sugiura, Y Nishiyama, Y |
| description | S Asano, T Honda, F Goshima, D Watanabe, Y Miyake, Y Sugiura and Y Nishiyama
Research Institute for Disease Mechanism and Control, Department of Ophthalmology, Nagoya University School of Medicine, Japan.
To clarify the biological role of US3 protein kinase of herpes simplex
virus type 2 (HSV-2) in vivo, the expression of the viral antigen, the
appearance of apoptotic bodies and DNA fragmentation were examined
immunohistologically after corneal infection of mice with three different
kinds of HSV-2 strain 186: the wild-type virus, a US3- deficient mutant
(L1BR1) and its revertant (L1B-11). In both wild-type 186- and
L1B-11-infected mice, viral antigen was diffusely found in the corneal
epithelium; no apoptotic changes were detected in the epithelial cells.
Whereas, in L1BR1-infected mice, HSV-immunoreactivity was localized around
the virus-inoculated sites, and a large number of apoptotic bodies were
observed in the corneal epithelium with dual- positive reactions for both
HSV-immunostaining and TUNEL staining. These results suggest that the US3
protein kinase plays an important role in protecting HSV-2-infected cells
from apoptotic death in vivo. |
| doi_str_mv | 10.1099/0022-1317-80-1-51 |
| format | Article |
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Research Institute for Disease Mechanism and Control, Department of Ophthalmology, Nagoya University School of Medicine, Japan.
To clarify the biological role of US3 protein kinase of herpes simplex
virus type 2 (HSV-2) in vivo, the expression of the viral antigen, the
appearance of apoptotic bodies and DNA fragmentation were examined
immunohistologically after corneal infection of mice with three different
kinds of HSV-2 strain 186: the wild-type virus, a US3- deficient mutant
(L1BR1) and its revertant (L1B-11). In both wild-type 186- and
L1B-11-infected mice, viral antigen was diffusely found in the corneal
epithelium; no apoptotic changes were detected in the epithelial cells.
Whereas, in L1BR1-infected mice, HSV-immunoreactivity was localized around
the virus-inoculated sites, and a large number of apoptotic bodies were
observed in the corneal epithelium with dual- positive reactions for both
HSV-immunostaining and TUNEL staining. These results suggest that the US3
protein kinase plays an important role in protecting HSV-2-infected cells
from apoptotic death in vivo.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-80-1-51</identifier><identifier>PMID: 9934683</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Animals ; Apoptosis ; Disease Models, Animal ; Epithelial Cells - cytology ; Epithelium, Corneal - cytology ; Epithelium, Corneal - immunology ; Herpes Genitalis - immunology ; Herpes Genitalis - pathology ; Herpes Genitalis - virology ; Herpes simplex virus 2 ; Herpesvirus 2, Human - enzymology ; Herpesvirus 2, Human - genetics ; Herpesvirus 2, Human - immunology ; Humans ; Male ; Mice ; Mice, Inbred ICR ; Mutagenesis ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - immunology ; Protein-Serine-Threonine Kinases - physiology ; Rabbits ; Viral Proteins</subject><ispartof>Journal of general virology, 1999-01, Vol.80 (1), p.51-56</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-fe1a366e5976073675a6d16bb6e5aa19aaa2326514fc0b95a1d5247474953f3c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3732,3733,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9934683$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asano, S</creatorcontrib><creatorcontrib>Honda, T</creatorcontrib><creatorcontrib>Goshima, F</creatorcontrib><creatorcontrib>Watanabe, D</creatorcontrib><creatorcontrib>Miyake, Y</creatorcontrib><creatorcontrib>Sugiura, Y</creatorcontrib><creatorcontrib>Nishiyama, Y</creatorcontrib><title>US3 protein kinase of herpes simplex virus type 2 plays a role in protecting corneal epithelial cells from apoptosis in infected mice</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>S Asano, T Honda, F Goshima, D Watanabe, Y Miyake, Y Sugiura and Y Nishiyama
Research Institute for Disease Mechanism and Control, Department of Ophthalmology, Nagoya University School of Medicine, Japan.
To clarify the biological role of US3 protein kinase of herpes simplex
virus type 2 (HSV-2) in vivo, the expression of the viral antigen, the
appearance of apoptotic bodies and DNA fragmentation were examined
immunohistologically after corneal infection of mice with three different
kinds of HSV-2 strain 186: the wild-type virus, a US3- deficient mutant
(L1BR1) and its revertant (L1B-11). In both wild-type 186- and
L1B-11-infected mice, viral antigen was diffusely found in the corneal
epithelium; no apoptotic changes were detected in the epithelial cells.
Whereas, in L1BR1-infected mice, HSV-immunoreactivity was localized around
the virus-inoculated sites, and a large number of apoptotic bodies were
observed in the corneal epithelium with dual- positive reactions for both
HSV-immunostaining and TUNEL staining. These results suggest that the US3
protein kinase plays an important role in protecting HSV-2-infected cells
from apoptotic death in vivo.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Disease Models, Animal</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelium, Corneal - cytology</subject><subject>Epithelium, Corneal - immunology</subject><subject>Herpes Genitalis - immunology</subject><subject>Herpes Genitalis - pathology</subject><subject>Herpes Genitalis - virology</subject><subject>Herpes simplex virus 2</subject><subject>Herpesvirus 2, Human - enzymology</subject><subject>Herpesvirus 2, Human - genetics</subject><subject>Herpesvirus 2, Human - immunology</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Mutagenesis</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Protein-Serine-Threonine Kinases - immunology</subject><subject>Protein-Serine-Threonine Kinases - physiology</subject><subject>Rabbits</subject><subject>Viral Proteins</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9u1DAQxq0KVLaFB-CANCduAU8c2-sjqvoHqRIH6NlyspNdlyQ2drZlH6DvjUOXojl4NDO_b8YfY--Rf0JuzGfO67pCgbpa8woriSdshY2SVV26r9jqpf-GneV8zzk2jdSn7NQY0ai1WLGnu-8CYgoz-Ql--sllgtDDjlKkDNmPcaDf8ODTPsN8iAQ1xMEdMjhIYSAo1F-6m_20hS6kidwAFP28o8GXtKNhyNCnMIKLIc4h-7xQfuoLRBsYfUdv2eveDZneHd9zdnd1-ePiprr9dv314stt1TVKz1VP6IRSJI1WXAulpVMbVG1bSs6hcc7VolYSm77jrZEON7JudAkjRS86cc4-PuuWm3_tKc929Hm50E0U9tmiruW6MboM4vNgl0LOiXobkx9dOljkdrHeLtbaxVq7LhUrsTAfjuL7dqTNC3H0-v_ynd_uHn0iu6Wp_D6F1gdbLP4n9AfK-Y2p</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Asano, S</creator><creator>Honda, T</creator><creator>Goshima, F</creator><creator>Watanabe, D</creator><creator>Miyake, Y</creator><creator>Sugiura, Y</creator><creator>Nishiyama, Y</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>19990101</creationdate><title>US3 protein kinase of herpes simplex virus type 2 plays a role in protecting corneal epithelial cells from apoptosis in infected mice</title><author>Asano, S ; Honda, T ; Goshima, F ; Watanabe, D ; Miyake, Y ; Sugiura, Y ; Nishiyama, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-fe1a366e5976073675a6d16bb6e5aa19aaa2326514fc0b95a1d5247474953f3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Disease Models, Animal</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelium, Corneal - cytology</topic><topic>Epithelium, Corneal - immunology</topic><topic>Herpes Genitalis - immunology</topic><topic>Herpes Genitalis - pathology</topic><topic>Herpes Genitalis - virology</topic><topic>Herpes simplex virus 2</topic><topic>Herpesvirus 2, Human - enzymology</topic><topic>Herpesvirus 2, Human - genetics</topic><topic>Herpesvirus 2, Human - immunology</topic><topic>Humans</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Mutagenesis</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Protein-Serine-Threonine Kinases - immunology</topic><topic>Protein-Serine-Threonine Kinases - physiology</topic><topic>Rabbits</topic><topic>Viral Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asano, S</creatorcontrib><creatorcontrib>Honda, T</creatorcontrib><creatorcontrib>Goshima, F</creatorcontrib><creatorcontrib>Watanabe, D</creatorcontrib><creatorcontrib>Miyake, Y</creatorcontrib><creatorcontrib>Sugiura, Y</creatorcontrib><creatorcontrib>Nishiyama, Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asano, S</au><au>Honda, T</au><au>Goshima, F</au><au>Watanabe, D</au><au>Miyake, Y</au><au>Sugiura, Y</au><au>Nishiyama, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>US3 protein kinase of herpes simplex virus type 2 plays a role in protecting corneal epithelial cells from apoptosis in infected mice</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>80</volume><issue>1</issue><spage>51</spage><epage>56</epage><pages>51-56</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>S Asano, T Honda, F Goshima, D Watanabe, Y Miyake, Y Sugiura and Y Nishiyama
Research Institute for Disease Mechanism and Control, Department of Ophthalmology, Nagoya University School of Medicine, Japan.
To clarify the biological role of US3 protein kinase of herpes simplex
virus type 2 (HSV-2) in vivo, the expression of the viral antigen, the
appearance of apoptotic bodies and DNA fragmentation were examined
immunohistologically after corneal infection of mice with three different
kinds of HSV-2 strain 186: the wild-type virus, a US3- deficient mutant
(L1BR1) and its revertant (L1B-11). In both wild-type 186- and
L1B-11-infected mice, viral antigen was diffusely found in the corneal
epithelium; no apoptotic changes were detected in the epithelial cells.
Whereas, in L1BR1-infected mice, HSV-immunoreactivity was localized around
the virus-inoculated sites, and a large number of apoptotic bodies were
observed in the corneal epithelium with dual- positive reactions for both
HSV-immunostaining and TUNEL staining. These results suggest that the US3
protein kinase plays an important role in protecting HSV-2-infected cells
from apoptotic death in vivo.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>9934683</pmid><doi>10.1099/0022-1317-80-1-51</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Apoptosis Disease Models, Animal Epithelial Cells - cytology Epithelium, Corneal - cytology Epithelium, Corneal - immunology Herpes Genitalis - immunology Herpes Genitalis - pathology Herpes Genitalis - virology Herpes simplex virus 2 Herpesvirus 2, Human - enzymology Herpesvirus 2, Human - genetics Herpesvirus 2, Human - immunology Humans Male Mice Mice, Inbred ICR Mutagenesis Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - immunology Protein-Serine-Threonine Kinases - physiology Rabbits Viral Proteins |
| title | US3 protein kinase of herpes simplex virus type 2 plays a role in protecting corneal epithelial cells from apoptosis in infected mice |
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