Induction of Arylhydrocarbon Receptor Expression in Embryoblast Cells of Rabbit Preimplantation Blastocysts upon Degeneration of Rauber's Polar Trophoblast
The arylhydrocarbon receptor (AhR) is a ligand-activated transcription factor and mediates carcinogenic, teratogenic, and toxic effects of xenobiotics such as dioxin and coplanar polychlorinated biphenyls. The AhR nuclear translocator (ARNT) is involved in AhR signal transduction. We have analyzed t...
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| Veröffentlicht in: | Toxicology and applied pharmacology 1999-06, Vol.157 (2), p.125-133 |
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| creator | Tscheudschilsuren, G. Küchenhoff, A. Klonisch, T. Tetens, F. Fischer, B. |
| description | The arylhydrocarbon receptor (AhR) is a ligand-activated transcription factor and mediates carcinogenic, teratogenic, and toxic effects of xenobiotics such as dioxin and coplanar polychlorinated biphenyls. The AhR nuclear translocator (ARNT) is involved in AhR signal transduction. We have analyzed the expression of AhR and ARNT mRNA and AhR protein in Day 3 pc (postcoitum) rabbit morulae and Days 4 and 6 pc blastocysts using RT–PCR, nested PCR, whole mount in situ hybridization, and whole mount immunohistochemistry with subsequent confocal laser scanning analysis. AhR and ARNT transcripts were detected in all stages investigated, indicating coexpression of both transcription factors. AhR protein was localized in the cytoplasm. It was detected in Day 3 pc morulae and in blastocysts. In Day 4 pc blastocysts, only trophoblast cells but not embryoblast cells were immunopositive. However, at Day 6 pc, the embryoblast cells also expressed AhR protein and this expression was correlated with the degeneration of Rauber's trophoblast layer. |
| doi_str_mv | 10.1006/taap.1999.8667 |
| format | Article |
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The AhR nuclear translocator (ARNT) is involved in AhR signal transduction. We have analyzed the expression of AhR and ARNT mRNA and AhR protein in Day 3 pc (postcoitum) rabbit morulae and Days 4 and 6 pc blastocysts using RT–PCR, nested PCR, whole mount in situ hybridization, and whole mount immunohistochemistry with subsequent confocal laser scanning analysis. AhR and ARNT transcripts were detected in all stages investigated, indicating coexpression of both transcription factors. AhR protein was localized in the cytoplasm. It was detected in Day 3 pc morulae and in blastocysts. In Day 4 pc blastocysts, only trophoblast cells but not embryoblast cells were immunopositive. However, at Day 6 pc, the embryoblast cells also expressed AhR protein and this expression was correlated with the degeneration of Rauber's trophoblast layer.</description><identifier>ISSN: 0041-008X</identifier><identifier>EISSN: 1096-0333</identifier><identifier>DOI: 10.1006/taap.1999.8667</identifier><identifier>PMID: 10366545</identifier><identifier>CODEN: TXAPA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>AhR nuclear translocator (ARNT) ; Animals ; Aryl Hydrocarbon Receptor Nuclear Translocator ; arylhydrocarbon receptor (AhR) ; Biological and medical sciences ; Blastocyst - chemistry ; Blastocyst - cytology ; Blastocyst - metabolism ; Blotting, Western ; Cytoplasm - chemistry ; Cytoplasm - metabolism ; DNA-Binding Proteins ; Embryonic Development ; Environmental pollutants toxicology ; Female ; Gene Expression Regulation, Developmental ; General aspects ; Immunohistochemistry ; In Situ Hybridization ; Medical sciences ; Pregnancy ; preimplantation embryos ; rabbit ; Rabbits ; Rauber's polar trophoblast ; Receptors, Aryl Hydrocarbon - analysis ; Receptors, Aryl Hydrocarbon - genetics ; Receptors, Aryl Hydrocarbon - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Sequence Homology, Nucleic Acid ; Toxicology ; Transcription Factors - genetics ; Trophoblasts - cytology ; Trophoblasts - metabolism ; Trophoblasts - physiology</subject><ispartof>Toxicology and applied pharmacology, 1999-06, Vol.157 (2), p.125-133</ispartof><rights>1999 Academic Press</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-8769a16182ac1500d7f7d1ce1818b29f4774bee66efc907eac892d03bac4e4863</citedby><cites>FETCH-LOGICAL-c466t-8769a16182ac1500d7f7d1ce1818b29f4774bee66efc907eac892d03bac4e4863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0041008X99986672$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1850574$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10366545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tscheudschilsuren, G.</creatorcontrib><creatorcontrib>Küchenhoff, A.</creatorcontrib><creatorcontrib>Klonisch, T.</creatorcontrib><creatorcontrib>Tetens, F.</creatorcontrib><creatorcontrib>Fischer, B.</creatorcontrib><title>Induction of Arylhydrocarbon Receptor Expression in Embryoblast Cells of Rabbit Preimplantation Blastocysts upon Degeneration of Rauber's Polar Trophoblast</title><title>Toxicology and applied pharmacology</title><addtitle>Toxicol Appl Pharmacol</addtitle><description>The arylhydrocarbon receptor (AhR) is a ligand-activated transcription factor and mediates carcinogenic, teratogenic, and toxic effects of xenobiotics such as dioxin and coplanar polychlorinated biphenyls. The AhR nuclear translocator (ARNT) is involved in AhR signal transduction. We have analyzed the expression of AhR and ARNT mRNA and AhR protein in Day 3 pc (postcoitum) rabbit morulae and Days 4 and 6 pc blastocysts using RT–PCR, nested PCR, whole mount in situ hybridization, and whole mount immunohistochemistry with subsequent confocal laser scanning analysis. AhR and ARNT transcripts were detected in all stages investigated, indicating coexpression of both transcription factors. AhR protein was localized in the cytoplasm. It was detected in Day 3 pc morulae and in blastocysts. In Day 4 pc blastocysts, only trophoblast cells but not embryoblast cells were immunopositive. However, at Day 6 pc, the embryoblast cells also expressed AhR protein and this expression was correlated with the degeneration of Rauber's trophoblast layer.</description><subject>AhR nuclear translocator (ARNT)</subject><subject>Animals</subject><subject>Aryl Hydrocarbon Receptor Nuclear Translocator</subject><subject>arylhydrocarbon receptor (AhR)</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - chemistry</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - metabolism</subject><subject>Blotting, Western</subject><subject>Cytoplasm - chemistry</subject><subject>Cytoplasm - metabolism</subject><subject>DNA-Binding Proteins</subject><subject>Embryonic Development</subject><subject>Environmental pollutants toxicology</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>General aspects</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Medical sciences</subject><subject>Pregnancy</subject><subject>preimplantation embryos</subject><subject>rabbit</subject><subject>Rabbits</subject><subject>Rauber's polar trophoblast</subject><subject>Receptors, Aryl Hydrocarbon - analysis</subject><subject>Receptors, Aryl Hydrocarbon - genetics</subject><subject>Receptors, Aryl Hydrocarbon - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Toxicology</subject><subject>Transcription Factors - genetics</subject><subject>Trophoblasts - cytology</subject><subject>Trophoblasts - metabolism</subject><subject>Trophoblasts - physiology</subject><issn>0041-008X</issn><issn>1096-0333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv1DAUhSMEokNhyxJlgegqg504fizbYYBKlahGRWJnXTs31CgTB9tB5Lf0z5KQQbBhdaWr75z7OFn2kpItJYS_TQDDliqltpJz8SjbUKJ4QaqqepxtCGG0IER-OcuexfiNEKIYo0-zM0oqzmtWb7KH674ZbXK-z32bX4apu5-a4C0EM7cOaHFIPuT7n0PAGBfM9fn-aMLkTQcx5TvsurhoD2CMS_ltQHccOugT_Ha9Wihvp5hiPg5z4x1-xR4D_Jl5gNFguIj5re8g5HfBD_er9_PsSQtdxBenep59fr-_230sbj59uN5d3hSWcZ4KKbgCyqkswdKakEa0oqEWqaTSlKplQjCDyDm2VhGBYKUqG1IZsAyZ5NV59mb1HYL_PmJM-uiine-CHv0YNRVlXSqxgNsVtMHHGLDVQ3BHCJOmRC9x6CUOvcShlzhmwauT82iO2PyDr_-fgdcnAKKFrg3QWxf_crImtWAzJlcM5zf8cBh0tA57i40LaJNuvPvfCr8ArDOqaQ</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Tscheudschilsuren, G.</creator><creator>Küchenhoff, A.</creator><creator>Klonisch, T.</creator><creator>Tetens, F.</creator><creator>Fischer, B.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19990601</creationdate><title>Induction of Arylhydrocarbon Receptor Expression in Embryoblast Cells of Rabbit Preimplantation Blastocysts upon Degeneration of Rauber's Polar Trophoblast</title><author>Tscheudschilsuren, G. ; Küchenhoff, A. ; Klonisch, T. ; Tetens, F. ; Fischer, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-8769a16182ac1500d7f7d1ce1818b29f4774bee66efc907eac892d03bac4e4863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>AhR nuclear translocator (ARNT)</topic><topic>Animals</topic><topic>Aryl Hydrocarbon Receptor Nuclear Translocator</topic><topic>arylhydrocarbon receptor (AhR)</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - chemistry</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - metabolism</topic><topic>Blotting, Western</topic><topic>Cytoplasm - chemistry</topic><topic>Cytoplasm - metabolism</topic><topic>DNA-Binding Proteins</topic><topic>Embryonic Development</topic><topic>Environmental pollutants toxicology</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental</topic><topic>General aspects</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Medical sciences</topic><topic>Pregnancy</topic><topic>preimplantation embryos</topic><topic>rabbit</topic><topic>Rabbits</topic><topic>Rauber's polar trophoblast</topic><topic>Receptors, Aryl Hydrocarbon - analysis</topic><topic>Receptors, Aryl Hydrocarbon - genetics</topic><topic>Receptors, Aryl Hydrocarbon - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Toxicology</topic><topic>Transcription Factors - genetics</topic><topic>Trophoblasts - cytology</topic><topic>Trophoblasts - metabolism</topic><topic>Trophoblasts - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tscheudschilsuren, G.</creatorcontrib><creatorcontrib>Küchenhoff, A.</creatorcontrib><creatorcontrib>Klonisch, T.</creatorcontrib><creatorcontrib>Tetens, F.</creatorcontrib><creatorcontrib>Fischer, B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology and applied pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tscheudschilsuren, G.</au><au>Küchenhoff, A.</au><au>Klonisch, T.</au><au>Tetens, F.</au><au>Fischer, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Arylhydrocarbon Receptor Expression in Embryoblast Cells of Rabbit Preimplantation Blastocysts upon Degeneration of Rauber's Polar Trophoblast</atitle><jtitle>Toxicology and applied pharmacology</jtitle><addtitle>Toxicol Appl Pharmacol</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>157</volume><issue>2</issue><spage>125</spage><epage>133</epage><pages>125-133</pages><issn>0041-008X</issn><eissn>1096-0333</eissn><coden>TXAPA9</coden><abstract>The arylhydrocarbon receptor (AhR) is a ligand-activated transcription factor and mediates carcinogenic, teratogenic, and toxic effects of xenobiotics such as dioxin and coplanar polychlorinated biphenyls. The AhR nuclear translocator (ARNT) is involved in AhR signal transduction. We have analyzed the expression of AhR and ARNT mRNA and AhR protein in Day 3 pc (postcoitum) rabbit morulae and Days 4 and 6 pc blastocysts using RT–PCR, nested PCR, whole mount in situ hybridization, and whole mount immunohistochemistry with subsequent confocal laser scanning analysis. AhR and ARNT transcripts were detected in all stages investigated, indicating coexpression of both transcription factors. AhR protein was localized in the cytoplasm. It was detected in Day 3 pc morulae and in blastocysts. In Day 4 pc blastocysts, only trophoblast cells but not embryoblast cells were immunopositive. However, at Day 6 pc, the embryoblast cells also expressed AhR protein and this expression was correlated with the degeneration of Rauber's trophoblast layer.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>10366545</pmid><doi>10.1006/taap.1999.8667</doi><tpages>9</tpages></addata></record> |
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| ispartof | Toxicology and applied pharmacology, 1999-06, Vol.157 (2), p.125-133 |
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| subjects | AhR nuclear translocator (ARNT) Animals Aryl Hydrocarbon Receptor Nuclear Translocator arylhydrocarbon receptor (AhR) Biological and medical sciences Blastocyst - chemistry Blastocyst - cytology Blastocyst - metabolism Blotting, Western Cytoplasm - chemistry Cytoplasm - metabolism DNA-Binding Proteins Embryonic Development Environmental pollutants toxicology Female Gene Expression Regulation, Developmental General aspects Immunohistochemistry In Situ Hybridization Medical sciences Pregnancy preimplantation embryos rabbit Rabbits Rauber's polar trophoblast Receptors, Aryl Hydrocarbon - analysis Receptors, Aryl Hydrocarbon - genetics Receptors, Aryl Hydrocarbon - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Homology, Nucleic Acid Toxicology Transcription Factors - genetics Trophoblasts - cytology Trophoblasts - metabolism Trophoblasts - physiology |
| title | Induction of Arylhydrocarbon Receptor Expression in Embryoblast Cells of Rabbit Preimplantation Blastocysts upon Degeneration of Rauber's Polar Trophoblast |
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