Evaluation of serum carbohydrate-deficient transferrin by HPLC and MALDI-TOF MS
The percentage of carbohydrate-deficient transferrin (%CDT) in serum is a marker of habitual alcohol intake that can be determined by antibody detection of abnormal disialo sugar chains at D432 and D630. However, this approach lacks specificity for alcoholic liver disease. To decrease the false-posi...
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| Veröffentlicht in: | Clinica chimica acta 2015-08, Vol.448, p.8-12 |
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| container_title | Clinica chimica acta |
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| creator | Sogawa, Kazuyuki Iida, Fumie Kawshima, Yusuke Yamada, Mako Satoh, Mamoru Sanda, Akihiro Takizawa, Hirotaka Maruyama, Katsuya Wada, Yoshinao Nomura, Fumio |
| description | The percentage of carbohydrate-deficient transferrin (%CDT) in serum is a marker of habitual alcohol intake that can be determined by antibody detection of abnormal disialo sugar chains at D432 and D630. However, this approach lacks specificity for alcoholic liver disease. To decrease the false-positive rate in patients with non-alcoholic liver diseases, we developed a screening method using the disialo sugar chain at D630 alone.
Serum was obtained from 12 patients with alcoholic liver disease, 12 with type C chronic liver disease, 6 with non-alcoholic steatohepatitis (NASH), and 12 healthy non-alcohol drinkers. Transferrin with two sialic acids (disialotransferrin) was fractionated from serum using HPLC, digested with trypsin, and evaluated using MALDI-TOF MS.
An abnormal sugar chain at D630 of transferrin was not detected in healthy subjects or in patients with chronic liver disease or NASH, but was detected in 9 patients (75%) with alcoholic liver disease. Positive results were found in 3 samples that were negative using an N-Latex CDT kit and in one sample negative for γ-glutamylaminotransferase and CDT.
Detection of CDT by HPLC/MALDI-TOF MS based on an abnormal sugar chain at D630 may permit identification of habitual alcohol drinkers when used in combination with current markers. |
| doi_str_mv | 10.1016/j.cca.2015.06.005 |
| format | Article |
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Serum was obtained from 12 patients with alcoholic liver disease, 12 with type C chronic liver disease, 6 with non-alcoholic steatohepatitis (NASH), and 12 healthy non-alcohol drinkers. Transferrin with two sialic acids (disialotransferrin) was fractionated from serum using HPLC, digested with trypsin, and evaluated using MALDI-TOF MS.
An abnormal sugar chain at D630 of transferrin was not detected in healthy subjects or in patients with chronic liver disease or NASH, but was detected in 9 patients (75%) with alcoholic liver disease. Positive results were found in 3 samples that were negative using an N-Latex CDT kit and in one sample negative for γ-glutamylaminotransferase and CDT.
Detection of CDT by HPLC/MALDI-TOF MS based on an abnormal sugar chain at D630 may permit identification of habitual alcohol drinkers when used in combination with current markers.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/j.cca.2015.06.005</identifier><identifier>PMID: 26092603</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Alcohol ; Chromatography, High Pressure Liquid ; Disialotransferrin ; Female ; HPLC ; Humans ; Liver Diseases, Alcoholic - blood ; Liver Diseases, Alcoholic - diagnosis ; Liver Diseases, Alcoholic - metabolism ; MALDI-TOF MS ; Male ; Middle Aged ; Serum ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Transferrin - analogs & derivatives ; Transferrin - analysis ; Transferrin - metabolism</subject><ispartof>Clinica chimica acta, 2015-08, Vol.448, p.8-12</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-551ed700ba6daafcd47c476bfe661b05aa65012b1d9fd0f2f6403e2a7a4840713</citedby><cites>FETCH-LOGICAL-c419t-551ed700ba6daafcd47c476bfe661b05aa65012b1d9fd0f2f6403e2a7a4840713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0009898115002855$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26092603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sogawa, Kazuyuki</creatorcontrib><creatorcontrib>Iida, Fumie</creatorcontrib><creatorcontrib>Kawshima, Yusuke</creatorcontrib><creatorcontrib>Yamada, Mako</creatorcontrib><creatorcontrib>Satoh, Mamoru</creatorcontrib><creatorcontrib>Sanda, Akihiro</creatorcontrib><creatorcontrib>Takizawa, Hirotaka</creatorcontrib><creatorcontrib>Maruyama, Katsuya</creatorcontrib><creatorcontrib>Wada, Yoshinao</creatorcontrib><creatorcontrib>Nomura, Fumio</creatorcontrib><title>Evaluation of serum carbohydrate-deficient transferrin by HPLC and MALDI-TOF MS</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>The percentage of carbohydrate-deficient transferrin (%CDT) in serum is a marker of habitual alcohol intake that can be determined by antibody detection of abnormal disialo sugar chains at D432 and D630. However, this approach lacks specificity for alcoholic liver disease. To decrease the false-positive rate in patients with non-alcoholic liver diseases, we developed a screening method using the disialo sugar chain at D630 alone.
Serum was obtained from 12 patients with alcoholic liver disease, 12 with type C chronic liver disease, 6 with non-alcoholic steatohepatitis (NASH), and 12 healthy non-alcohol drinkers. Transferrin with two sialic acids (disialotransferrin) was fractionated from serum using HPLC, digested with trypsin, and evaluated using MALDI-TOF MS.
An abnormal sugar chain at D630 of transferrin was not detected in healthy subjects or in patients with chronic liver disease or NASH, but was detected in 9 patients (75%) with alcoholic liver disease. Positive results were found in 3 samples that were negative using an N-Latex CDT kit and in one sample negative for γ-glutamylaminotransferase and CDT.
Detection of CDT by HPLC/MALDI-TOF MS based on an abnormal sugar chain at D630 may permit identification of habitual alcohol drinkers when used in combination with current markers.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Alcohol</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Disialotransferrin</subject><subject>Female</subject><subject>HPLC</subject><subject>Humans</subject><subject>Liver Diseases, Alcoholic - blood</subject><subject>Liver Diseases, Alcoholic - diagnosis</subject><subject>Liver Diseases, Alcoholic - metabolism</subject><subject>MALDI-TOF MS</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Serum</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Transferrin - analogs & derivatives</subject><subject>Transferrin - analysis</subject><subject>Transferrin - metabolism</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRtH78AC-yRy-Js8lm0-BJ6ie0VLCel8nuLG5pE91NCv33plQ9ehiGged9YR7GLgWkAoS6WabGYJqBKFJQKUBxwEZiXOZJLqvskI0AoErG1VicsNMYl8MpQYljdpIpqIbJR2z-sMFVj51vG946Hin0a24w1O3H1gbsKLHkvPHUdLwL2ERHIfiG11v-_DqdcGwsn91N71-SxfyRz97O2ZHDVaSLn33G3h8fFpPnZDp_epncTRMjRdUlRSHIlgA1KovojJWlkaWqHSklaigQVQEiq4WtnAWXOSUhpwxLlGMJpcjP2PW-9zO0Xz3FTq99NLRaYUNtH7UoswKyIpflgIo9akIbYyCnP4NfY9hqAXrnUS_14FHvPGpQevA4ZK5-6vt6TfYv8StuAG73AA1PbjwFHXeSDFkfyHTatv6f-m9b2IFe</recordid><startdate>20150825</startdate><enddate>20150825</enddate><creator>Sogawa, Kazuyuki</creator><creator>Iida, Fumie</creator><creator>Kawshima, Yusuke</creator><creator>Yamada, Mako</creator><creator>Satoh, Mamoru</creator><creator>Sanda, Akihiro</creator><creator>Takizawa, Hirotaka</creator><creator>Maruyama, Katsuya</creator><creator>Wada, Yoshinao</creator><creator>Nomura, Fumio</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150825</creationdate><title>Evaluation of serum carbohydrate-deficient transferrin by HPLC and MALDI-TOF MS</title><author>Sogawa, Kazuyuki ; Iida, Fumie ; Kawshima, Yusuke ; Yamada, Mako ; Satoh, Mamoru ; Sanda, Akihiro ; Takizawa, Hirotaka ; Maruyama, Katsuya ; Wada, Yoshinao ; Nomura, Fumio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-551ed700ba6daafcd47c476bfe661b05aa65012b1d9fd0f2f6403e2a7a4840713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Alcohol</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Disialotransferrin</topic><topic>Female</topic><topic>HPLC</topic><topic>Humans</topic><topic>Liver Diseases, Alcoholic - blood</topic><topic>Liver Diseases, Alcoholic - diagnosis</topic><topic>Liver Diseases, Alcoholic - metabolism</topic><topic>MALDI-TOF MS</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Serum</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Transferrin - analogs & derivatives</topic><topic>Transferrin - analysis</topic><topic>Transferrin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sogawa, Kazuyuki</creatorcontrib><creatorcontrib>Iida, Fumie</creatorcontrib><creatorcontrib>Kawshima, Yusuke</creatorcontrib><creatorcontrib>Yamada, Mako</creatorcontrib><creatorcontrib>Satoh, Mamoru</creatorcontrib><creatorcontrib>Sanda, Akihiro</creatorcontrib><creatorcontrib>Takizawa, Hirotaka</creatorcontrib><creatorcontrib>Maruyama, Katsuya</creatorcontrib><creatorcontrib>Wada, Yoshinao</creatorcontrib><creatorcontrib>Nomura, Fumio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sogawa, Kazuyuki</au><au>Iida, Fumie</au><au>Kawshima, Yusuke</au><au>Yamada, Mako</au><au>Satoh, Mamoru</au><au>Sanda, Akihiro</au><au>Takizawa, Hirotaka</au><au>Maruyama, Katsuya</au><au>Wada, Yoshinao</au><au>Nomura, Fumio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of serum carbohydrate-deficient transferrin by HPLC and MALDI-TOF MS</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>2015-08-25</date><risdate>2015</risdate><volume>448</volume><spage>8</spage><epage>12</epage><pages>8-12</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>The percentage of carbohydrate-deficient transferrin (%CDT) in serum is a marker of habitual alcohol intake that can be determined by antibody detection of abnormal disialo sugar chains at D432 and D630. However, this approach lacks specificity for alcoholic liver disease. To decrease the false-positive rate in patients with non-alcoholic liver diseases, we developed a screening method using the disialo sugar chain at D630 alone.
Serum was obtained from 12 patients with alcoholic liver disease, 12 with type C chronic liver disease, 6 with non-alcoholic steatohepatitis (NASH), and 12 healthy non-alcohol drinkers. Transferrin with two sialic acids (disialotransferrin) was fractionated from serum using HPLC, digested with trypsin, and evaluated using MALDI-TOF MS.
An abnormal sugar chain at D630 of transferrin was not detected in healthy subjects or in patients with chronic liver disease or NASH, but was detected in 9 patients (75%) with alcoholic liver disease. Positive results were found in 3 samples that were negative using an N-Latex CDT kit and in one sample negative for γ-glutamylaminotransferase and CDT.
Detection of CDT by HPLC/MALDI-TOF MS based on an abnormal sugar chain at D630 may permit identification of habitual alcohol drinkers when used in combination with current markers.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26092603</pmid><doi>10.1016/j.cca.2015.06.005</doi><tpages>5</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Alcohol Chromatography, High Pressure Liquid Disialotransferrin Female HPLC Humans Liver Diseases, Alcoholic - blood Liver Diseases, Alcoholic - diagnosis Liver Diseases, Alcoholic - metabolism MALDI-TOF MS Male Middle Aged Serum Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Transferrin - analogs & derivatives Transferrin - analysis Transferrin - metabolism |
| title | Evaluation of serum carbohydrate-deficient transferrin by HPLC and MALDI-TOF MS |
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