A survey of growth stimulators in natural rubber serum powder for Bifidobacterium bifidum
Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum, which was unable to grow in a fully synthetic medium, B12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cut of...
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| Veröffentlicht in: | Journal of the Faculty of Agriculture, Kyushu University Kyushu University, 1999-02, Vol.43 (3-4), p.451-460 |
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| container_title | Journal of the Faculty of Agriculture, Kyushu University |
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| creator | Etoh, S. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) Sonomoto, K Ishizaki, A |
| description | Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum, which was unable to grow in a fully synthetic medium, B12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cut off 1000). The active ultrafiltrate was furthermore concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. Through this purification step, it was found that the adsorbed fraction obtained could not exhibit a growth-stimulating activity independently but acted complementarily with ammonium sulfate contaminated in the ultrafiltrate. Furthermore,ammonium sulfate could be substituted with other ammonium salts, ammonium chloride and ammonium nitrate. The adsorbed fraction was subsequently analyzed on reversed-phase high Performance liquid chromatography, and the activities of the eluates were measured on B12 assay medium supplemented with ammonium sulfate. Consequently, it was proved that several ingredients in the adsorbed fraction enhanced the growth of B. bifidum |
| doi_str_mv | 10.5109/24292 |
| format | Article |
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(Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) ; Sonomoto, K ; Ishizaki, A</creator><creatorcontrib>Etoh, S. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) ; Sonomoto, K ; Ishizaki, A</creatorcontrib><description>Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum, which was unable to grow in a fully synthetic medium, B12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cut off 1000). The active ultrafiltrate was furthermore concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. Through this purification step, it was found that the adsorbed fraction obtained could not exhibit a growth-stimulating activity independently but acted complementarily with ammonium sulfate contaminated in the ultrafiltrate. Furthermore,ammonium sulfate could be substituted with other ammonium salts, ammonium chloride and ammonium nitrate. The adsorbed fraction was subsequently analyzed on reversed-phase high Performance liquid chromatography, and the activities of the eluates were measured on B12 assay medium supplemented with ammonium sulfate. Consequently, it was proved that several ingredients in the adsorbed fraction enhanced the growth of B. bifidum</description><identifier>ISSN: 0023-6152</identifier><identifier>DOI: 10.5109/24292</identifier><language>eng</language><subject>ADSORCION ; ADSORPTION ; BIFIDOBACTERIUM ; Bifidobacterium bifidum ; CAOUTCHOUC ; CAUCHO ; ESTIMULANTES DE CRECIMIENTO VEGETAL ; PLANT GROWTH STIMULANTS ; POLVOS (FORMULACIONES) ; POUDRE ; POWDERS ; RUBBER ; STIMULANT DE CROISSANCE VEGETALE ; ULTRAFILTRACION ; ULTRAFILTRATION</subject><ispartof>Journal of the Faculty of Agriculture, Kyushu University, 1999-02, Vol.43 (3-4), p.451-460</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Etoh, S. 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Through this purification step, it was found that the adsorbed fraction obtained could not exhibit a growth-stimulating activity independently but acted complementarily with ammonium sulfate contaminated in the ultrafiltrate. Furthermore,ammonium sulfate could be substituted with other ammonium salts, ammonium chloride and ammonium nitrate. The adsorbed fraction was subsequently analyzed on reversed-phase high Performance liquid chromatography, and the activities of the eluates were measured on B12 assay medium supplemented with ammonium sulfate. 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Through this purification step, it was found that the adsorbed fraction obtained could not exhibit a growth-stimulating activity independently but acted complementarily with ammonium sulfate contaminated in the ultrafiltrate. Furthermore,ammonium sulfate could be substituted with other ammonium salts, ammonium chloride and ammonium nitrate. The adsorbed fraction was subsequently analyzed on reversed-phase high Performance liquid chromatography, and the activities of the eluates were measured on B12 assay medium supplemented with ammonium sulfate. Consequently, it was proved that several ingredients in the adsorbed fraction enhanced the growth of B. bifidum</abstract><doi>10.5109/24292</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0023-6152 |
| ispartof | Journal of the Faculty of Agriculture, Kyushu University, 1999-02, Vol.43 (3-4), p.451-460 |
| issn | 0023-6152 |
| language | eng |
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| source | Open Access Titles of Japan; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | ADSORCION ADSORPTION BIFIDOBACTERIUM Bifidobacterium bifidum CAOUTCHOUC CAUCHO ESTIMULANTES DE CRECIMIENTO VEGETAL PLANT GROWTH STIMULANTS POLVOS (FORMULACIONES) POUDRE POWDERS RUBBER STIMULANT DE CROISSANCE VEGETALE ULTRAFILTRACION ULTRAFILTRATION |
| title | A survey of growth stimulators in natural rubber serum powder for Bifidobacterium bifidum |
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