Enhancement of Veratridine-Induced Sodium Dynamics in NG108-15 Cells during Differentiation
Developmental changes in dynamics of Na+ were studied in neuroblastoma×glioma hybrid NG108-15 cells during differentiation which was induced by dibutyryl cAMP (Bt2cAMP). Ratiometric Na+ imaging with a Na+-sensitive fluorescent dye SBFI (sodium-binding benzofuran isophthalate) revealed that the intra...
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| Veröffentlicht in: | Biological & Pharmaceutical Bulletin 2006, Vol.29(4), pp.701-704 |
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| creator | Imanishi, Takashi Matsushima, Kayoko Kawaguchi, Akinori Wada, Tetsuyuki Masuko, Takashi Yoshida, Shigeru Ichida, Seiji |
| description | Developmental changes in dynamics of Na+ were studied in neuroblastoma×glioma hybrid NG108-15 cells during differentiation which was induced by dibutyryl cAMP (Bt2cAMP). Ratiometric Na+ imaging with a Na+-sensitive fluorescent dye SBFI (sodium-binding benzofuran isophthalate) revealed that the intracellular Na+ concentration ([Na+]i) was not affected by the application of high K+ (60 mM) solution to either control or differentiated cells. When cells were exposed to 50 μM veratridine (Vtd), an agonist of voltage-sensitive sodium channels (VSSCs), a significant increase in [Na+]i was observed in differentiated but not in undifferentiated cells. Calculated mean [Na+]i value increased from the basal 10.4 to 44.1 mM in response to 50 μM Vtd. This Vtd response was reversibly inhibited by tetrodotoxin (TTX), a specific blocker for VSSCs, in a dose-dependent manner (IC50=1 nM). It is suggested that VSSCs in NG108-15 cells are sensitive to TTX and Vtd and that the number of VSSCs increases during differentiation. |
| doi_str_mv | 10.1248/bpb.29.701 |
| format | Article |
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Ratiometric Na+ imaging with a Na+-sensitive fluorescent dye SBFI (sodium-binding benzofuran isophthalate) revealed that the intracellular Na+ concentration ([Na+]i) was not affected by the application of high K+ (60 mM) solution to either control or differentiated cells. When cells were exposed to 50 μM veratridine (Vtd), an agonist of voltage-sensitive sodium channels (VSSCs), a significant increase in [Na+]i was observed in differentiated but not in undifferentiated cells. Calculated mean [Na+]i value increased from the basal 10.4 to 44.1 mM in response to 50 μM Vtd. This Vtd response was reversibly inhibited by tetrodotoxin (TTX), a specific blocker for VSSCs, in a dose-dependent manner (IC50=1 nM). It is suggested that VSSCs in NG108-15 cells are sensitive to TTX and Vtd and that the number of VSSCs increases during differentiation.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.29.701</identifier><identifier>PMID: 16595902</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Benzofurans ; Bucladesine - pharmacology ; Cell Differentiation - drug effects ; Cell Line ; differentiation ; Dose-Response Relationship, Drug ; Ethers, Cyclic ; Fluorescent Dyes ; Humans ; Neurons - drug effects ; NG108-15 cell ; Potassium - pharmacology ; Sodium - metabolism ; sodium channel ; Sodium Channel Agonists ; Sodium Channel Blockers - pharmacology ; sodium-binding benzofuran isophthalate (SBFI) ; tetrodotoxin ; Tetrodotoxin - pharmacology ; veratridine ; Veratridine - pharmacology</subject><ispartof>Biological and Pharmaceutical Bulletin, 2006, Vol.29(4), pp.701-704</ispartof><rights>2006 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c714t-3c50a8c2deff58a5450d22e0dbd2517aa1fd7bc3c303c9db771be3cf5bc211513</citedby><cites>FETCH-LOGICAL-c714t-3c50a8c2deff58a5450d22e0dbd2517aa1fd7bc3c303c9db771be3cf5bc211513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16595902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imanishi, Takashi</creatorcontrib><creatorcontrib>Matsushima, Kayoko</creatorcontrib><creatorcontrib>Kawaguchi, Akinori</creatorcontrib><creatorcontrib>Wada, Tetsuyuki</creatorcontrib><creatorcontrib>Masuko, Takashi</creatorcontrib><creatorcontrib>Yoshida, Shigeru</creatorcontrib><creatorcontrib>Ichida, Seiji</creatorcontrib><creatorcontrib>School of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>cDepartment of Life Science</creatorcontrib><creatorcontrib>aDepartment of Biological Chemistry</creatorcontrib><creatorcontrib>bDepartment of Cell Biology</creatorcontrib><creatorcontrib>School of Science & Engineering</creatorcontrib><creatorcontrib>Kinki University</creatorcontrib><title>Enhancement of Veratridine-Induced Sodium Dynamics in NG108-15 Cells during Differentiation</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Developmental changes in dynamics of Na+ were studied in neuroblastoma×glioma hybrid NG108-15 cells during differentiation which was induced by dibutyryl cAMP (Bt2cAMP). Ratiometric Na+ imaging with a Na+-sensitive fluorescent dye SBFI (sodium-binding benzofuran isophthalate) revealed that the intracellular Na+ concentration ([Na+]i) was not affected by the application of high K+ (60 mM) solution to either control or differentiated cells. When cells were exposed to 50 μM veratridine (Vtd), an agonist of voltage-sensitive sodium channels (VSSCs), a significant increase in [Na+]i was observed in differentiated but not in undifferentiated cells. Calculated mean [Na+]i value increased from the basal 10.4 to 44.1 mM in response to 50 μM Vtd. This Vtd response was reversibly inhibited by tetrodotoxin (TTX), a specific blocker for VSSCs, in a dose-dependent manner (IC50=1 nM). It is suggested that VSSCs in NG108-15 cells are sensitive to TTX and Vtd and that the number of VSSCs increases during differentiation.</description><subject>Benzofurans</subject><subject>Bucladesine - pharmacology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>differentiation</subject><subject>Dose-Response Relationship, Drug</subject><subject>Ethers, Cyclic</subject><subject>Fluorescent Dyes</subject><subject>Humans</subject><subject>Neurons - drug effects</subject><subject>NG108-15 cell</subject><subject>Potassium - pharmacology</subject><subject>Sodium - metabolism</subject><subject>sodium channel</subject><subject>Sodium Channel Agonists</subject><subject>Sodium Channel Blockers - pharmacology</subject><subject>sodium-binding benzofuran isophthalate (SBFI)</subject><subject>tetrodotoxin</subject><subject>Tetrodotoxin - pharmacology</subject><subject>veratridine</subject><subject>Veratridine - pharmacology</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1v1DAQhiMEokvhwg9AkZA4IGWZ8cc6PiG03ZZKFRz4uHCwHNtpvUrsxU4O_fd4lW0rcZk5zDPvvHqnqt4irJGw9lN36NZErgXgs2qFlImGE-TPqxVIbJsN8vasepXzHgAEEPqyOsMNl1wCWVV_duFOB-NGF6Y69vVvl_SUvPXBNdfBzsbZ-ke0fh7ri_ugR29y7UP97QqhbZDXWzcMubZz8uG2vvB971JR8nryMbyuXvR6yO7NqZ9Xvy53P7dfm5vvV9fbLzeNEcimhhoOujXEur7nreaMgyXEge0s4Si0xt6KzlBDgRppOyGwc9T0vDMEkSM9rz4suocU_84uT2r02RRjOrg4Z4WCMCKlKOD7_8B9nFMo3hQyJilvOZeF-rhQJsWck-vVIflRp3uFoI6BqxK4IlKVwAv87iQ5d6OzT-gp4QLsFqBMvdFDDEPJ9umwyaLzcYiKAGwUAJHAFKBQ5Vd4LIxsWtgUnc-Lzj5P-tY9HtJp8mZwD57YUo67DxNzp5Nygf4DB0Sncg</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Imanishi, Takashi</creator><creator>Matsushima, Kayoko</creator><creator>Kawaguchi, Akinori</creator><creator>Wada, Tetsuyuki</creator><creator>Masuko, Takashi</creator><creator>Yoshida, Shigeru</creator><creator>Ichida, Seiji</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20060401</creationdate><title>Enhancement of Veratridine-Induced Sodium Dynamics in NG108-15 Cells during Differentiation</title><author>Imanishi, Takashi ; Matsushima, Kayoko ; Kawaguchi, Akinori ; Wada, Tetsuyuki ; Masuko, Takashi ; Yoshida, Shigeru ; Ichida, Seiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c714t-3c50a8c2deff58a5450d22e0dbd2517aa1fd7bc3c303c9db771be3cf5bc211513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Benzofurans</topic><topic>Bucladesine - pharmacology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>differentiation</topic><topic>Dose-Response Relationship, Drug</topic><topic>Ethers, Cyclic</topic><topic>Fluorescent Dyes</topic><topic>Humans</topic><topic>Neurons - drug effects</topic><topic>NG108-15 cell</topic><topic>Potassium - pharmacology</topic><topic>Sodium - metabolism</topic><topic>sodium channel</topic><topic>Sodium Channel Agonists</topic><topic>Sodium Channel Blockers - pharmacology</topic><topic>sodium-binding benzofuran isophthalate (SBFI)</topic><topic>tetrodotoxin</topic><topic>Tetrodotoxin - pharmacology</topic><topic>veratridine</topic><topic>Veratridine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Imanishi, Takashi</creatorcontrib><creatorcontrib>Matsushima, Kayoko</creatorcontrib><creatorcontrib>Kawaguchi, Akinori</creatorcontrib><creatorcontrib>Wada, Tetsuyuki</creatorcontrib><creatorcontrib>Masuko, Takashi</creatorcontrib><creatorcontrib>Yoshida, Shigeru</creatorcontrib><creatorcontrib>Ichida, Seiji</creatorcontrib><creatorcontrib>School of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>cDepartment of Life Science</creatorcontrib><creatorcontrib>aDepartment of Biological Chemistry</creatorcontrib><creatorcontrib>bDepartment of Cell Biology</creatorcontrib><creatorcontrib>School of Science & Engineering</creatorcontrib><creatorcontrib>Kinki University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Biological & Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imanishi, Takashi</au><au>Matsushima, Kayoko</au><au>Kawaguchi, Akinori</au><au>Wada, Tetsuyuki</au><au>Masuko, Takashi</au><au>Yoshida, Shigeru</au><au>Ichida, Seiji</au><aucorp>School of Pharmaceutical Sciences</aucorp><aucorp>cDepartment of Life Science</aucorp><aucorp>aDepartment of Biological Chemistry</aucorp><aucorp>bDepartment of Cell Biology</aucorp><aucorp>School of Science & Engineering</aucorp><aucorp>Kinki University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of Veratridine-Induced Sodium Dynamics in NG108-15 Cells during Differentiation</atitle><jtitle>Biological & Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>29</volume><issue>4</issue><spage>701</spage><epage>704</epage><pages>701-704</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Developmental changes in dynamics of Na+ were studied in neuroblastoma×glioma hybrid NG108-15 cells during differentiation which was induced by dibutyryl cAMP (Bt2cAMP). Ratiometric Na+ imaging with a Na+-sensitive fluorescent dye SBFI (sodium-binding benzofuran isophthalate) revealed that the intracellular Na+ concentration ([Na+]i) was not affected by the application of high K+ (60 mM) solution to either control or differentiated cells. When cells were exposed to 50 μM veratridine (Vtd), an agonist of voltage-sensitive sodium channels (VSSCs), a significant increase in [Na+]i was observed in differentiated but not in undifferentiated cells. Calculated mean [Na+]i value increased from the basal 10.4 to 44.1 mM in response to 50 μM Vtd. This Vtd response was reversibly inhibited by tetrodotoxin (TTX), a specific blocker for VSSCs, in a dose-dependent manner (IC50=1 nM). It is suggested that VSSCs in NG108-15 cells are sensitive to TTX and Vtd and that the number of VSSCs increases during differentiation.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>16595902</pmid><doi>10.1248/bpb.29.701</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Benzofurans Bucladesine - pharmacology Cell Differentiation - drug effects Cell Line differentiation Dose-Response Relationship, Drug Ethers, Cyclic Fluorescent Dyes Humans Neurons - drug effects NG108-15 cell Potassium - pharmacology Sodium - metabolism sodium channel Sodium Channel Agonists Sodium Channel Blockers - pharmacology sodium-binding benzofuran isophthalate (SBFI) tetrodotoxin Tetrodotoxin - pharmacology veratridine Veratridine - pharmacology |
| title | Enhancement of Veratridine-Induced Sodium Dynamics in NG108-15 Cells during Differentiation |
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