Sensitive Quantitation of Chromium-DNA Adducts by Inductively Coupled Plasma Mass Spectrometry with a Direct Injection High-Efficiency Nebulizer
A novel method is described for the sensitive detection of chromium-DNA adducts. Chromium-DNA adducts were determined in 1 μg of DNA from normal human lung fibroblasts exposed to sodium chromate using microscale flow injection analysis with a direct injection high-efficiency nebulizer and inductivel...
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| Veröffentlicht in: | Toxicological sciences 1998-12, Vol.46 (2), p.260-265 |
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| container_title | Toxicological sciences |
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| creator | Singh, Jatinder McLean, John A. Pritchard, Daryl E. Montaser, Akbar Patierno, Steven R. |
| description | A novel method is described for the sensitive detection of chromium-DNA adducts. Chromium-DNA adducts were determined in 1 μg of DNA from normal human lung fibroblasts exposed to sodium chromate using microscale flow injection analysis with a direct injection high-efficiency nebulizer and inductively coupled plasma mass spectrometry detection. The frequency of Cr-DNA adducts increased in a dose-dependent sigmoidal manner, indicating saturation and toxicity. The low detection limits (on the order of parts per trillion) allows the detection of as few as two Cr adducts per 10,000 bases, which, coupled with the small DNA sample requirement, makes this technique suitable for measuring metal-DNA adducts as biomarkers of exposure to toxic and carcinogenic metals such as Cr, in cultured cells, animals, and humans. |
| doi_str_mv | 10.1093/toxsci/46.2.260 |
| format | Article |
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The low detection limits (on the order of parts per trillion) allows the detection of as few as two Cr adducts per 10,000 bases, which, coupled with the small DNA sample requirement, makes this technique suitable for measuring metal-DNA adducts as biomarkers of exposure to toxic and carcinogenic metals such as Cr, in cultured cells, animals, and humans.</description><identifier>ISSN: 1096-6080</identifier><identifier>EISSN: 1096-0929</identifier><identifier>DOI: 10.1093/toxsci/46.2.260</identifier><identifier>PMID: 10048129</identifier><identifier>CODEN: TOSCF2</identifier><language>eng</language><publisher>Cary, NC: Oxford University Press</publisher><subject>Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Chemical agents ; Chromium - metabolism ; DNA Adducts - analysis ; DNA Adducts - isolation & purification ; Dose-Response Relationship, Drug ; Fibroblasts ; Humans ; Lung - cytology ; Mass Spectrometry - methods ; Medical sciences ; Nebulizers and Vaporizers ; Sensitivity and Specificity ; Tumors</subject><ispartof>Toxicological sciences, 1998-12, Vol.46 (2), p.260-265</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-cc85db0ac725dc38373a596a9f4cd662d7c95593b2eddcd852e7de05c0cad4693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1816417$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10048129$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Jatinder</creatorcontrib><creatorcontrib>McLean, John A.</creatorcontrib><creatorcontrib>Pritchard, Daryl E.</creatorcontrib><creatorcontrib>Montaser, Akbar</creatorcontrib><creatorcontrib>Patierno, Steven R.</creatorcontrib><title>Sensitive Quantitation of Chromium-DNA Adducts by Inductively Coupled Plasma Mass Spectrometry with a Direct Injection High-Efficiency Nebulizer</title><title>Toxicological sciences</title><addtitle>Toxicol Sci</addtitle><description>A novel method is described for the sensitive detection of chromium-DNA adducts. Chromium-DNA adducts were determined in 1 μg of DNA from normal human lung fibroblasts exposed to sodium chromate using microscale flow injection analysis with a direct injection high-efficiency nebulizer and inductively coupled plasma mass spectrometry detection. The frequency of Cr-DNA adducts increased in a dose-dependent sigmoidal manner, indicating saturation and toxicity. The low detection limits (on the order of parts per trillion) allows the detection of as few as two Cr adducts per 10,000 bases, which, coupled with the small DNA sample requirement, makes this technique suitable for measuring metal-DNA adducts as biomarkers of exposure to toxic and carcinogenic metals such as Cr, in cultured cells, animals, and humans.</description><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Chemical agents</subject><subject>Chromium - metabolism</subject><subject>DNA Adducts - analysis</subject><subject>DNA Adducts - isolation & purification</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fibroblasts</subject><subject>Humans</subject><subject>Lung - cytology</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Nebulizers and Vaporizers</subject><subject>Sensitivity and Specificity</subject><subject>Tumors</subject><issn>1096-6080</issn><issn>1096-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkUtv1DAUhS0EoqWwZoe8QOwy40fixMvRtHSK2kJVQBUby7l2GJc8BtuBhl_BT8ajjIDVPbr3O2dxD0IvKVlQIvkyDg8B3DIXC7ZggjxCx2ktMiKZfHzQglTkCD0L4Z4QSgWRT9ERJSSvKJPH6Pet7YOL7ofFN6Puo4s6uqHHQ4PXWz90buyy0-sVXhkzQgy4nvBFv5fJ0U54PYy71hr8odWh0_hKh4BvdxZistroJ_zTxS3W-NT5tEzW-zT2-Rv3dZudNY0DZ3uY8LWtx9b9sv45etLoNtgXh3mCPr09-7jeZJfvzy_Wq8sMuMhjBlAVpiYaSlYY4BUvuS6k0LLJwQjBTAmyKCSvmTUGTFUwWxpLCiCgTS4kP0Fv5tydH76PNkTVuQC2bXVvhzEoWjIueMUSuJxB8EMI3jZq512n_aQoUfsS1FyCyoViKpWQHK8O0WPdWfMfP389Aa8PgA6g28brHlz4x1VU5LRMWDZjLkT78Pes_TclSl4WanP3RV1tPpfn7-5ulOR_AD8Jo10</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Singh, Jatinder</creator><creator>McLean, John A.</creator><creator>Pritchard, Daryl E.</creator><creator>Montaser, Akbar</creator><creator>Patierno, Steven R.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19981201</creationdate><title>Sensitive Quantitation of Chromium-DNA Adducts by Inductively Coupled Plasma Mass Spectrometry with a Direct Injection High-Efficiency Nebulizer</title><author>Singh, Jatinder ; McLean, John A. ; Pritchard, Daryl E. ; Montaser, Akbar ; Patierno, Steven R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-cc85db0ac725dc38373a596a9f4cd662d7c95593b2eddcd852e7de05c0cad4693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Chemical agents</topic><topic>Chromium - metabolism</topic><topic>DNA Adducts - analysis</topic><topic>DNA Adducts - isolation & purification</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fibroblasts</topic><topic>Humans</topic><topic>Lung - cytology</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Nebulizers and Vaporizers</topic><topic>Sensitivity and Specificity</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Jatinder</creatorcontrib><creatorcontrib>McLean, John A.</creatorcontrib><creatorcontrib>Pritchard, Daryl E.</creatorcontrib><creatorcontrib>Montaser, Akbar</creatorcontrib><creatorcontrib>Patierno, Steven R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Jatinder</au><au>McLean, John A.</au><au>Pritchard, Daryl E.</au><au>Montaser, Akbar</au><au>Patierno, Steven R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive Quantitation of Chromium-DNA Adducts by Inductively Coupled Plasma Mass Spectrometry with a Direct Injection High-Efficiency Nebulizer</atitle><jtitle>Toxicological sciences</jtitle><addtitle>Toxicol Sci</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>46</volume><issue>2</issue><spage>260</spage><epage>265</epage><pages>260-265</pages><issn>1096-6080</issn><eissn>1096-0929</eissn><coden>TOSCF2</coden><abstract>A novel method is described for the sensitive detection of chromium-DNA adducts. Chromium-DNA adducts were determined in 1 μg of DNA from normal human lung fibroblasts exposed to sodium chromate using microscale flow injection analysis with a direct injection high-efficiency nebulizer and inductively coupled plasma mass spectrometry detection. The frequency of Cr-DNA adducts increased in a dose-dependent sigmoidal manner, indicating saturation and toxicity. The low detection limits (on the order of parts per trillion) allows the detection of as few as two Cr adducts per 10,000 bases, which, coupled with the small DNA sample requirement, makes this technique suitable for measuring metal-DNA adducts as biomarkers of exposure to toxic and carcinogenic metals such as Cr, in cultured cells, animals, and humans.</abstract><cop>Cary, NC</cop><pub>Oxford University Press</pub><pmid>10048129</pmid><doi>10.1093/toxsci/46.2.260</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1096-6080 |
| ispartof | Toxicological sciences, 1998-12, Vol.46 (2), p.260-265 |
| issn | 1096-6080 1096-0929 |
| language | eng |
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| source | MEDLINE; Full-Text Journals in Chemistry (Open access); OUP_牛津大学出版社现刊; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Chemical agents Chromium - metabolism DNA Adducts - analysis DNA Adducts - isolation & purification Dose-Response Relationship, Drug Fibroblasts Humans Lung - cytology Mass Spectrometry - methods Medical sciences Nebulizers and Vaporizers Sensitivity and Specificity Tumors |
| title | Sensitive Quantitation of Chromium-DNA Adducts by Inductively Coupled Plasma Mass Spectrometry with a Direct Injection High-Efficiency Nebulizer |
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