Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)
The purpose of this study was to quantify antibiotic resistance genes (ARG) in the sediments of the mixed-landscape Cache La Poudre River, which has previously been studied and shown to have high concentrations of antibiotics related to urban and agricultural activities. River sediments were sampled...
Gespeichert in:
| Veröffentlicht in: | Water research (Oxford) 2006-07, Vol.40 (12), p.2427-2435 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2435 |
|---|---|
| container_issue | 12 |
| container_start_page | 2427 |
| container_title | Water research (Oxford) |
| container_volume | 40 |
| creator | Pei, Ruoting Kim, Sung-Chul Carlson, Kenneth H. Pruden, Amy |
| description | The purpose of this study was to quantify antibiotic resistance genes (ARG) in the sediments of the mixed-landscape Cache La Poudre River, which has previously been studied and shown to have high concentrations of antibiotics related to urban and agricultural activities. River sediments were sampled during two events (high-flow and low-flow) from five sites with varying urban and agricultural impact levels. Polymerase-chain-reaction (PCR) detection assays were conducted for four sulfonamide resistance gene families, using newly designed primers, and five tetracycline resistance gene families, using previously published primers. S
ul(I),
sul(II),
tet(W), and
tet(O) gene families were further quantified by real-time quantitative polymerase chain reaction (Q-PCR). Resistance to four classes of antibiotics (tetracyclines, sulfonamides, ionophores, and macrolides) was also investigated using a culture-based approach. The quantities of resistance genes normalized to the 16S gene copy number were significantly different between the sites, with higher resistance gene concentrations at the impacted sites than at the pristine site. Total resistant CFUs were over an order of magnitude lower at the pristine site, but differences were less apparent when normalized to the total CFUs. Six tetracyclines and six sulfonamides were also quantified in the sediments and were found to be highest at sites impacted by urban and agricultural activity, with no antibiotics detected at the pristine sit. To the knowledge of the authors, this study is the first to demonstrate a relationship between urban and agricultural activity and microbial resistance in river sediments using quantitative molecular tools. |
| doi_str_mv | 10.1016/j.watres.2006.04.017 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17232075</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0043135406002338</els_id><sourcerecordid>14776320</sourcerecordid><originalsourceid>FETCH-LOGICAL-c542t-143ba295f8816c4f1b737fc7556f0b283bc0ba05947523fd4524dbd07362d2913</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhoMo7rj6D0RzUfQwbeWjk-7LwrKsqzAgrO45pPMxZphJxiSzIv55M_TAetJTQup5i0o9CL0k0BEg4sOm-6lrdqWjAKID3gGRj9CCDHJcUs6Hx2gBwNmSsJ6foWelbACAUjY-RWdEyJ6RUS7Q72vvnak4eXwb7l3GKx1tMXrvcIq4fne4OBt2LlZsUjTtzLqGFMsxoWMNU0g1mNLuthG5DbRP0Ya4_quK22soVbc8XrvoCn53eXvz_jl64vW2uBen8xzdfbz-dvVpufpy8_nqcrU0Pad1STibNB17PwxEGO7JJJn0Rva98DDRgU0GJg39yGVPmbe8p9xOFiQT1NKRsHP0du67z-nHwZWqdqEYt93q6NKhKCIpo9A28l-QSyka2kA-gyanUrLzap_DTudfioA62lEbNdtRRzsKuGp2WuzVqf9h2jn7EDrpaMCbE6Cbg63PbWWhPHByBMLE8UevZ87rpPQ6N-buK201IDAIAWMjLmbCtcXeB5dVMcE1ATbk5lvZFP496x-bHrkq</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14776320</pqid></control><display><type>article</type><title>Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Pei, Ruoting ; Kim, Sung-Chul ; Carlson, Kenneth H. ; Pruden, Amy</creator><creatorcontrib>Pei, Ruoting ; Kim, Sung-Chul ; Carlson, Kenneth H. ; Pruden, Amy</creatorcontrib><description>The purpose of this study was to quantify antibiotic resistance genes (ARG) in the sediments of the mixed-landscape Cache La Poudre River, which has previously been studied and shown to have high concentrations of antibiotics related to urban and agricultural activities. River sediments were sampled during two events (high-flow and low-flow) from five sites with varying urban and agricultural impact levels. Polymerase-chain-reaction (PCR) detection assays were conducted for four sulfonamide resistance gene families, using newly designed primers, and five tetracycline resistance gene families, using previously published primers. S
ul(I),
sul(II),
tet(W), and
tet(O) gene families were further quantified by real-time quantitative polymerase chain reaction (Q-PCR). Resistance to four classes of antibiotics (tetracyclines, sulfonamides, ionophores, and macrolides) was also investigated using a culture-based approach. The quantities of resistance genes normalized to the 16S gene copy number were significantly different between the sites, with higher resistance gene concentrations at the impacted sites than at the pristine site. Total resistant CFUs were over an order of magnitude lower at the pristine site, but differences were less apparent when normalized to the total CFUs. Six tetracyclines and six sulfonamides were also quantified in the sediments and were found to be highest at sites impacted by urban and agricultural activity, with no antibiotics detected at the pristine sit. To the knowledge of the authors, this study is the first to demonstrate a relationship between urban and agricultural activity and microbial resistance in river sediments using quantitative molecular tools.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/j.watres.2006.04.017</identifier><identifier>PMID: 16753197</identifier><identifier>CODEN: WATRAG</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>agricultural land ; agricultural watersheds ; Agriculture ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; antibiotics ; Applied sciences ; Cities ; Colorado ; DNA Primers ; DNA, Bacterial - genetics ; DNA, Bacterial - metabolism ; Drug Resistance, Bacterial ; Exact sciences and technology ; genes ; Genes, Bacterial - physiology ; Geologic Sediments ; ionophores ; landscape ecology ; macrolide antibiotics ; Macrolides ; microbial genetics ; Microbial Sensitivity Tests ; multigene family ; Other industrial wastes. Sewage sludge ; Pollution ; Polymerase Chain Reaction ; Quantification ; Reverse Transcriptase Polymerase Chain Reaction ; Rivers - microbiology ; RNA, Ribosomal, 16S ; sediment contamination ; Sediments ; Sulfonamides ; tetracycline ; Tetracycline Resistance ; Tetracyclines ; urban areas ; Waste Disposal, Fluid - methods ; Wastes ; Water Microbiology ; Water Pollutants, Chemical - analysis ; Water treatment and pollution</subject><ispartof>Water research (Oxford), 2006-07, Vol.40 (12), p.2427-2435</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-143ba295f8816c4f1b737fc7556f0b283bc0ba05947523fd4524dbd07362d2913</citedby><cites>FETCH-LOGICAL-c542t-143ba295f8816c4f1b737fc7556f0b283bc0ba05947523fd4524dbd07362d2913</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0043135406002338$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17901361$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16753197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pei, Ruoting</creatorcontrib><creatorcontrib>Kim, Sung-Chul</creatorcontrib><creatorcontrib>Carlson, Kenneth H.</creatorcontrib><creatorcontrib>Pruden, Amy</creatorcontrib><title>Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>The purpose of this study was to quantify antibiotic resistance genes (ARG) in the sediments of the mixed-landscape Cache La Poudre River, which has previously been studied and shown to have high concentrations of antibiotics related to urban and agricultural activities. River sediments were sampled during two events (high-flow and low-flow) from five sites with varying urban and agricultural impact levels. Polymerase-chain-reaction (PCR) detection assays were conducted for four sulfonamide resistance gene families, using newly designed primers, and five tetracycline resistance gene families, using previously published primers. S
ul(I),
sul(II),
tet(W), and
tet(O) gene families were further quantified by real-time quantitative polymerase chain reaction (Q-PCR). Resistance to four classes of antibiotics (tetracyclines, sulfonamides, ionophores, and macrolides) was also investigated using a culture-based approach. The quantities of resistance genes normalized to the 16S gene copy number were significantly different between the sites, with higher resistance gene concentrations at the impacted sites than at the pristine site. Total resistant CFUs were over an order of magnitude lower at the pristine site, but differences were less apparent when normalized to the total CFUs. Six tetracyclines and six sulfonamides were also quantified in the sediments and were found to be highest at sites impacted by urban and agricultural activity, with no antibiotics detected at the pristine sit. To the knowledge of the authors, this study is the first to demonstrate a relationship between urban and agricultural activity and microbial resistance in river sediments using quantitative molecular tools.</description><subject>agricultural land</subject><subject>agricultural watersheds</subject><subject>Agriculture</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>antibiotics</subject><subject>Applied sciences</subject><subject>Cities</subject><subject>Colorado</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - metabolism</subject><subject>Drug Resistance, Bacterial</subject><subject>Exact sciences and technology</subject><subject>genes</subject><subject>Genes, Bacterial - physiology</subject><subject>Geologic Sediments</subject><subject>ionophores</subject><subject>landscape ecology</subject><subject>macrolide antibiotics</subject><subject>Macrolides</subject><subject>microbial genetics</subject><subject>Microbial Sensitivity Tests</subject><subject>multigene family</subject><subject>Other industrial wastes. Sewage sludge</subject><subject>Pollution</subject><subject>Polymerase Chain Reaction</subject><subject>Quantification</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rivers - microbiology</subject><subject>RNA, Ribosomal, 16S</subject><subject>sediment contamination</subject><subject>Sediments</subject><subject>Sulfonamides</subject><subject>tetracycline</subject><subject>Tetracycline Resistance</subject><subject>Tetracyclines</subject><subject>urban areas</subject><subject>Waste Disposal, Fluid - methods</subject><subject>Wastes</subject><subject>Water Microbiology</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water treatment and pollution</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7rj6D0RzUfQwbeWjk-7LwrKsqzAgrO45pPMxZphJxiSzIv55M_TAetJTQup5i0o9CL0k0BEg4sOm-6lrdqWjAKID3gGRj9CCDHJcUs6Hx2gBwNmSsJ6foWelbACAUjY-RWdEyJ6RUS7Q72vvnak4eXwb7l3GKx1tMXrvcIq4fne4OBt2LlZsUjTtzLqGFMsxoWMNU0g1mNLuthG5DbRP0Ya4_quK22soVbc8XrvoCn53eXvz_jl64vW2uBen8xzdfbz-dvVpufpy8_nqcrU0Pad1STibNB17PwxEGO7JJJn0Rva98DDRgU0GJg39yGVPmbe8p9xOFiQT1NKRsHP0du67z-nHwZWqdqEYt93q6NKhKCIpo9A28l-QSyka2kA-gyanUrLzap_DTudfioA62lEbNdtRRzsKuGp2WuzVqf9h2jn7EDrpaMCbE6Cbg63PbWWhPHByBMLE8UevZ87rpPQ6N-buK201IDAIAWMjLmbCtcXeB5dVMcE1ATbk5lvZFP496x-bHrkq</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Pei, Ruoting</creator><creator>Kim, Sung-Chul</creator><creator>Carlson, Kenneth H.</creator><creator>Pruden, Amy</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7T7</scope><scope>7TV</scope><scope>7UA</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H96</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20060701</creationdate><title>Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)</title><author>Pei, Ruoting ; Kim, Sung-Chul ; Carlson, Kenneth H. ; Pruden, Amy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-143ba295f8816c4f1b737fc7556f0b283bc0ba05947523fd4524dbd07362d2913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>agricultural land</topic><topic>agricultural watersheds</topic><topic>Agriculture</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>antibiotics</topic><topic>Applied sciences</topic><topic>Cities</topic><topic>Colorado</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - metabolism</topic><topic>Drug Resistance, Bacterial</topic><topic>Exact sciences and technology</topic><topic>genes</topic><topic>Genes, Bacterial - physiology</topic><topic>Geologic Sediments</topic><topic>ionophores</topic><topic>landscape ecology</topic><topic>macrolide antibiotics</topic><topic>Macrolides</topic><topic>microbial genetics</topic><topic>Microbial Sensitivity Tests</topic><topic>multigene family</topic><topic>Other industrial wastes. Sewage sludge</topic><topic>Pollution</topic><topic>Polymerase Chain Reaction</topic><topic>Quantification</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rivers - microbiology</topic><topic>RNA, Ribosomal, 16S</topic><topic>sediment contamination</topic><topic>Sediments</topic><topic>Sulfonamides</topic><topic>tetracycline</topic><topic>Tetracycline Resistance</topic><topic>Tetracyclines</topic><topic>urban areas</topic><topic>Waste Disposal, Fluid - methods</topic><topic>Wastes</topic><topic>Water Microbiology</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pei, Ruoting</creatorcontrib><creatorcontrib>Kim, Sung-Chul</creatorcontrib><creatorcontrib>Carlson, Kenneth H.</creatorcontrib><creatorcontrib>Pruden, Amy</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pei, Ruoting</au><au>Kim, Sung-Chul</au><au>Carlson, Kenneth H.</au><au>Pruden, Amy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG)</atitle><jtitle>Water research (Oxford)</jtitle><addtitle>Water Res</addtitle><date>2006-07-01</date><risdate>2006</risdate><volume>40</volume><issue>12</issue><spage>2427</spage><epage>2435</epage><pages>2427-2435</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>The purpose of this study was to quantify antibiotic resistance genes (ARG) in the sediments of the mixed-landscape Cache La Poudre River, which has previously been studied and shown to have high concentrations of antibiotics related to urban and agricultural activities. River sediments were sampled during two events (high-flow and low-flow) from five sites with varying urban and agricultural impact levels. Polymerase-chain-reaction (PCR) detection assays were conducted for four sulfonamide resistance gene families, using newly designed primers, and five tetracycline resistance gene families, using previously published primers. S
ul(I),
sul(II),
tet(W), and
tet(O) gene families were further quantified by real-time quantitative polymerase chain reaction (Q-PCR). Resistance to four classes of antibiotics (tetracyclines, sulfonamides, ionophores, and macrolides) was also investigated using a culture-based approach. The quantities of resistance genes normalized to the 16S gene copy number were significantly different between the sites, with higher resistance gene concentrations at the impacted sites than at the pristine site. Total resistant CFUs were over an order of magnitude lower at the pristine site, but differences were less apparent when normalized to the total CFUs. Six tetracyclines and six sulfonamides were also quantified in the sediments and were found to be highest at sites impacted by urban and agricultural activity, with no antibiotics detected at the pristine sit. To the knowledge of the authors, this study is the first to demonstrate a relationship between urban and agricultural activity and microbial resistance in river sediments using quantitative molecular tools.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16753197</pmid><doi>10.1016/j.watres.2006.04.017</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0043-1354 |
| ispartof | Water research (Oxford), 2006-07, Vol.40 (12), p.2427-2435 |
| issn | 0043-1354 1879-2448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17232075 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | agricultural land agricultural watersheds Agriculture Anti-Bacterial Agents - pharmacology Antibiotic resistance antibiotics Applied sciences Cities Colorado DNA Primers DNA, Bacterial - genetics DNA, Bacterial - metabolism Drug Resistance, Bacterial Exact sciences and technology genes Genes, Bacterial - physiology Geologic Sediments ionophores landscape ecology macrolide antibiotics Macrolides microbial genetics Microbial Sensitivity Tests multigene family Other industrial wastes. Sewage sludge Pollution Polymerase Chain Reaction Quantification Reverse Transcriptase Polymerase Chain Reaction Rivers - microbiology RNA, Ribosomal, 16S sediment contamination Sediments Sulfonamides tetracycline Tetracycline Resistance Tetracyclines urban areas Waste Disposal, Fluid - methods Wastes Water Microbiology Water Pollutants, Chemical - analysis Water treatment and pollution |
| title | Effect of River Landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T19%3A37%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20River%20Landscape%20on%20the%20sediment%20concentrations%20of%20antibiotics%20and%20corresponding%20antibiotic%20resistance%20genes%20(ARG)&rft.jtitle=Water%20research%20(Oxford)&rft.au=Pei,%20Ruoting&rft.date=2006-07-01&rft.volume=40&rft.issue=12&rft.spage=2427&rft.epage=2435&rft.pages=2427-2435&rft.issn=0043-1354&rft.eissn=1879-2448&rft.coden=WATRAG&rft_id=info:doi/10.1016/j.watres.2006.04.017&rft_dat=%3Cproquest_cross%3E14776320%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14776320&rft_id=info:pmid/16753197&rft_els_id=S0043135406002338&rfr_iscdi=true |