Stimulatory effects of histamine on migration of nasal fibroblasts
Background Fibroblast migration is crucial for normal wound repair after sinonasal surgery. Histamine is known to be involved in wound healing by its effects on cell proliferation and migration. This study aimed to determine whether histamine affects the migration of nasal fibroblasts and to investi...
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| Veröffentlicht in: | International forum of allergy & rhinology 2015-10, Vol.5 (10), p.923-928 |
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| creator | Hong, Sung-Moon Park, Il-Ho Um, Ji-Young Shin, Jae-Min Lee, Heung-Man |
| description | Background
Fibroblast migration is crucial for normal wound repair after sinonasal surgery. Histamine is known to be involved in wound healing by its effects on cell proliferation and migration. This study aimed to determine whether histamine affects the migration of nasal fibroblasts and to investigate the mechanism of action of histamine on nasal fibroblasts.
Methods
Primary cultures of nasal fibroblasts were established from inferior turbinate samples. Fibroblast migration was evaluated with scratch assays. Cells were treated with histamine and/or histamine receptor‐selective antagonists. U‐73122 and pertussis toxin, which are selective inhibitors of the lower signaling pathway of H1R and H4R, were used to confirm the modulation of nasal fibroblast migration by histamine. Fibroblast cytoskeletal structures were visualized with immunocytochemistry.
Results
Histamine significantly stimulated the migration of nasal fibroblasts. Antagonists selective for HR1 and HR4 significantly reduced nasal fibroblast migration. In immunocytochemical staining, histamine treatment increased membrane ruffling and pyrilamine, diphenhydramine, fexofenadine, and JNJ7777120 decreased histamine‐induced membrane ruffling. U‐73122 and pertussis toxin also decreased histamine‐induced migration of fibroblasts. Histamine maintains its stimulatory effects on fibroblast migration in the presence of mitomycin C, which blocks proliferation of cells.
Conclusion
We showed that histamine stimulates fibroblast migration in nasal fibroblasts. This effect appeared to be mediated by HR1 and HR4. However, because fibroblast migration also can be involved in scaring and fibrosis, more research is necessary to determine the effects of antihistamine on wound healing after sinus surgery. |
| doi_str_mv | 10.1002/alr.21555 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1722933542</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1722933542</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4615-b132022f4007b20d7a32d37332b1496cffc768492a91ac19235da8bba2eb3f6b3</originalsourceid><addsrcrecordid>eNp10E1LBCEYB3CJomLr0BeIgS51mNLHUddjLb2yFfQKXURntax5KZ2h9ttnbe0hyIsP-Hv-yB-hDYJ3Ccawp6uwC4QxtoBWAReQczksFuez4CtoPcZnnA5LjIhltAIcSwGYraKD687XfaW7Nkwz65wtu5i1LnvysdO1b2zWNlntH4PufJrSS6OjrjLnTWhNpWMX19CS01W06z_3AN0eHd6MTvLx5fHpaH-clwUnLDeEAgZwBcbCAJ4ITWFCBaVgSCF56Vwp-LCQoCXRJZFA2UQPjdFgDXXc0AHanuW-hvatt7FTtY-lrSrd2LaPiggASSkrINGtP_S57UOTfvetiJQ8yQHamakytDEG69Rr8LUOU0Ww-upWpW7Vd7fJbv4k9qa2k7n8bTKBvRl495Wd_p-k9sdXv5H5bCNVbT_mGzq8KC6oYOr-4ljd86OHszsG6px-AuGOkDk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1722199693</pqid></control><display><type>article</type><title>Stimulatory effects of histamine on migration of nasal fibroblasts</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Hong, Sung-Moon ; Park, Il-Ho ; Um, Ji-Young ; Shin, Jae-Min ; Lee, Heung-Man</creator><creatorcontrib>Hong, Sung-Moon ; Park, Il-Ho ; Um, Ji-Young ; Shin, Jae-Min ; Lee, Heung-Man</creatorcontrib><description>Background
Fibroblast migration is crucial for normal wound repair after sinonasal surgery. Histamine is known to be involved in wound healing by its effects on cell proliferation and migration. This study aimed to determine whether histamine affects the migration of nasal fibroblasts and to investigate the mechanism of action of histamine on nasal fibroblasts.
Methods
Primary cultures of nasal fibroblasts were established from inferior turbinate samples. Fibroblast migration was evaluated with scratch assays. Cells were treated with histamine and/or histamine receptor‐selective antagonists. U‐73122 and pertussis toxin, which are selective inhibitors of the lower signaling pathway of H1R and H4R, were used to confirm the modulation of nasal fibroblast migration by histamine. Fibroblast cytoskeletal structures were visualized with immunocytochemistry.
Results
Histamine significantly stimulated the migration of nasal fibroblasts. Antagonists selective for HR1 and HR4 significantly reduced nasal fibroblast migration. In immunocytochemical staining, histamine treatment increased membrane ruffling and pyrilamine, diphenhydramine, fexofenadine, and JNJ7777120 decreased histamine‐induced membrane ruffling. U‐73122 and pertussis toxin also decreased histamine‐induced migration of fibroblasts. Histamine maintains its stimulatory effects on fibroblast migration in the presence of mitomycin C, which blocks proliferation of cells.
Conclusion
We showed that histamine stimulates fibroblast migration in nasal fibroblasts. This effect appeared to be mediated by HR1 and HR4. However, because fibroblast migration also can be involved in scaring and fibrosis, more research is necessary to determine the effects of antihistamine on wound healing after sinus surgery.</description><identifier>ISSN: 2042-6976</identifier><identifier>EISSN: 2042-6984</identifier><identifier>DOI: 10.1002/alr.21555</identifier><identifier>PMID: 26097205</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Cell Adhesion - drug effects ; Cell Adhesion - immunology ; Cell Movement - drug effects ; Cell Movement - immunology ; Cells, Cultured ; Cytoskeleton - metabolism ; Estrenes - pharmacology ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - physiology ; Histamine ; Histamine - immunology ; Histamine - metabolism ; histamine receptor ; Humans ; Immunization ; Indoles - pharmacology ; Migration ; nasal fibroblast ; Pertussis Toxin - pharmacology ; Piperazines - pharmacology ; Pyrrolidinones - pharmacology ; Receptors, G-Protein-Coupled - antagonists & inhibitors ; Receptors, Histamine ; Receptors, Histamine H4 ; Signal Transduction - drug effects ; Turbinates - cytology ; Wound healing</subject><ispartof>International forum of allergy & rhinology, 2015-10, Vol.5 (10), p.923-928</ispartof><rights>2015 ARS‐AAOA, LLC</rights><rights>2015 ARS-AAOA, LLC.</rights><rights>2015 ARS-AAOA, LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4615-b132022f4007b20d7a32d37332b1496cffc768492a91ac19235da8bba2eb3f6b3</citedby><cites>FETCH-LOGICAL-c4615-b132022f4007b20d7a32d37332b1496cffc768492a91ac19235da8bba2eb3f6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Falr.21555$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Falr.21555$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26097205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hong, Sung-Moon</creatorcontrib><creatorcontrib>Park, Il-Ho</creatorcontrib><creatorcontrib>Um, Ji-Young</creatorcontrib><creatorcontrib>Shin, Jae-Min</creatorcontrib><creatorcontrib>Lee, Heung-Man</creatorcontrib><title>Stimulatory effects of histamine on migration of nasal fibroblasts</title><title>International forum of allergy & rhinology</title><addtitle>International Forum of Allergy and Rhinology</addtitle><description>Background
Fibroblast migration is crucial for normal wound repair after sinonasal surgery. Histamine is known to be involved in wound healing by its effects on cell proliferation and migration. This study aimed to determine whether histamine affects the migration of nasal fibroblasts and to investigate the mechanism of action of histamine on nasal fibroblasts.
Methods
Primary cultures of nasal fibroblasts were established from inferior turbinate samples. Fibroblast migration was evaluated with scratch assays. Cells were treated with histamine and/or histamine receptor‐selective antagonists. U‐73122 and pertussis toxin, which are selective inhibitors of the lower signaling pathway of H1R and H4R, were used to confirm the modulation of nasal fibroblast migration by histamine. Fibroblast cytoskeletal structures were visualized with immunocytochemistry.
Results
Histamine significantly stimulated the migration of nasal fibroblasts. Antagonists selective for HR1 and HR4 significantly reduced nasal fibroblast migration. In immunocytochemical staining, histamine treatment increased membrane ruffling and pyrilamine, diphenhydramine, fexofenadine, and JNJ7777120 decreased histamine‐induced membrane ruffling. U‐73122 and pertussis toxin also decreased histamine‐induced migration of fibroblasts. Histamine maintains its stimulatory effects on fibroblast migration in the presence of mitomycin C, which blocks proliferation of cells.
Conclusion
We showed that histamine stimulates fibroblast migration in nasal fibroblasts. This effect appeared to be mediated by HR1 and HR4. However, because fibroblast migration also can be involved in scaring and fibrosis, more research is necessary to determine the effects of antihistamine on wound healing after sinus surgery.</description><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - immunology</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - immunology</subject><subject>Cells, Cultured</subject><subject>Cytoskeleton - metabolism</subject><subject>Estrenes - pharmacology</subject><subject>Fibroblasts</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - physiology</subject><subject>Histamine</subject><subject>Histamine - immunology</subject><subject>Histamine - metabolism</subject><subject>histamine receptor</subject><subject>Humans</subject><subject>Immunization</subject><subject>Indoles - pharmacology</subject><subject>Migration</subject><subject>nasal fibroblast</subject><subject>Pertussis Toxin - pharmacology</subject><subject>Piperazines - pharmacology</subject><subject>Pyrrolidinones - pharmacology</subject><subject>Receptors, G-Protein-Coupled - antagonists & inhibitors</subject><subject>Receptors, Histamine</subject><subject>Receptors, Histamine H4</subject><subject>Signal Transduction - drug effects</subject><subject>Turbinates - cytology</subject><subject>Wound healing</subject><issn>2042-6976</issn><issn>2042-6984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E1LBCEYB3CJomLr0BeIgS51mNLHUddjLb2yFfQKXURntax5KZ2h9ttnbe0hyIsP-Hv-yB-hDYJ3Ccawp6uwC4QxtoBWAReQczksFuez4CtoPcZnnA5LjIhltAIcSwGYraKD687XfaW7Nkwz65wtu5i1LnvysdO1b2zWNlntH4PufJrSS6OjrjLnTWhNpWMX19CS01W06z_3AN0eHd6MTvLx5fHpaH-clwUnLDeEAgZwBcbCAJ4ITWFCBaVgSCF56Vwp-LCQoCXRJZFA2UQPjdFgDXXc0AHanuW-hvatt7FTtY-lrSrd2LaPiggASSkrINGtP_S57UOTfvetiJQ8yQHamakytDEG69Rr8LUOU0Ww-upWpW7Vd7fJbv4k9qa2k7n8bTKBvRl495Wd_p-k9sdXv5H5bCNVbT_mGzq8KC6oYOr-4ljd86OHszsG6px-AuGOkDk</recordid><startdate>201510</startdate><enddate>201510</enddate><creator>Hong, Sung-Moon</creator><creator>Park, Il-Ho</creator><creator>Um, Ji-Young</creator><creator>Shin, Jae-Min</creator><creator>Lee, Heung-Man</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201510</creationdate><title>Stimulatory effects of histamine on migration of nasal fibroblasts</title><author>Hong, Sung-Moon ; Park, Il-Ho ; Um, Ji-Young ; Shin, Jae-Min ; Lee, Heung-Man</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4615-b132022f4007b20d7a32d37332b1496cffc768492a91ac19235da8bba2eb3f6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cell Adhesion - drug effects</topic><topic>Cell Adhesion - immunology</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - immunology</topic><topic>Cells, Cultured</topic><topic>Cytoskeleton - metabolism</topic><topic>Estrenes - pharmacology</topic><topic>Fibroblasts</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - physiology</topic><topic>Histamine</topic><topic>Histamine - immunology</topic><topic>Histamine - metabolism</topic><topic>histamine receptor</topic><topic>Humans</topic><topic>Immunization</topic><topic>Indoles - pharmacology</topic><topic>Migration</topic><topic>nasal fibroblast</topic><topic>Pertussis Toxin - pharmacology</topic><topic>Piperazines - pharmacology</topic><topic>Pyrrolidinones - pharmacology</topic><topic>Receptors, G-Protein-Coupled - antagonists & inhibitors</topic><topic>Receptors, Histamine</topic><topic>Receptors, Histamine H4</topic><topic>Signal Transduction - drug effects</topic><topic>Turbinates - cytology</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hong, Sung-Moon</creatorcontrib><creatorcontrib>Park, Il-Ho</creatorcontrib><creatorcontrib>Um, Ji-Young</creatorcontrib><creatorcontrib>Shin, Jae-Min</creatorcontrib><creatorcontrib>Lee, Heung-Man</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International forum of allergy & rhinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hong, Sung-Moon</au><au>Park, Il-Ho</au><au>Um, Ji-Young</au><au>Shin, Jae-Min</au><au>Lee, Heung-Man</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulatory effects of histamine on migration of nasal fibroblasts</atitle><jtitle>International forum of allergy & rhinology</jtitle><addtitle>International Forum of Allergy and Rhinology</addtitle><date>2015-10</date><risdate>2015</risdate><volume>5</volume><issue>10</issue><spage>923</spage><epage>928</epage><pages>923-928</pages><issn>2042-6976</issn><eissn>2042-6984</eissn><abstract>Background
Fibroblast migration is crucial for normal wound repair after sinonasal surgery. Histamine is known to be involved in wound healing by its effects on cell proliferation and migration. This study aimed to determine whether histamine affects the migration of nasal fibroblasts and to investigate the mechanism of action of histamine on nasal fibroblasts.
Methods
Primary cultures of nasal fibroblasts were established from inferior turbinate samples. Fibroblast migration was evaluated with scratch assays. Cells were treated with histamine and/or histamine receptor‐selective antagonists. U‐73122 and pertussis toxin, which are selective inhibitors of the lower signaling pathway of H1R and H4R, were used to confirm the modulation of nasal fibroblast migration by histamine. Fibroblast cytoskeletal structures were visualized with immunocytochemistry.
Results
Histamine significantly stimulated the migration of nasal fibroblasts. Antagonists selective for HR1 and HR4 significantly reduced nasal fibroblast migration. In immunocytochemical staining, histamine treatment increased membrane ruffling and pyrilamine, diphenhydramine, fexofenadine, and JNJ7777120 decreased histamine‐induced membrane ruffling. U‐73122 and pertussis toxin also decreased histamine‐induced migration of fibroblasts. Histamine maintains its stimulatory effects on fibroblast migration in the presence of mitomycin C, which blocks proliferation of cells.
Conclusion
We showed that histamine stimulates fibroblast migration in nasal fibroblasts. This effect appeared to be mediated by HR1 and HR4. However, because fibroblast migration also can be involved in scaring and fibrosis, more research is necessary to determine the effects of antihistamine on wound healing after sinus surgery.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>26097205</pmid><doi>10.1002/alr.21555</doi><tpages>6</tpages></addata></record> |
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| subjects | Cell Adhesion - drug effects Cell Adhesion - immunology Cell Movement - drug effects Cell Movement - immunology Cells, Cultured Cytoskeleton - metabolism Estrenes - pharmacology Fibroblasts Fibroblasts - drug effects Fibroblasts - physiology Histamine Histamine - immunology Histamine - metabolism histamine receptor Humans Immunization Indoles - pharmacology Migration nasal fibroblast Pertussis Toxin - pharmacology Piperazines - pharmacology Pyrrolidinones - pharmacology Receptors, G-Protein-Coupled - antagonists & inhibitors Receptors, Histamine Receptors, Histamine H4 Signal Transduction - drug effects Turbinates - cytology Wound healing |
| title | Stimulatory effects of histamine on migration of nasal fibroblasts |
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