T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates
Background and Objectives Regulatory T cells (Tregs) and other T‐cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autolo...
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| Veröffentlicht in: | Vox sanguinis 2015-11, Vol.109 (4), p.375-386 |
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| creator | Strobel, J. Moellmer, I. Zingsem, J. Hauck-Dlimi, B. Eckstein, R. Strasser, E. |
| description | Background and Objectives
Regulatory T cells (Tregs) and other T‐cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting.
Material and Methods
We characterized 53 donors, patients and peripheral blood stem cell concentrates (PBSC) regarding the content of CD45+ cells, lymphocytes, CD3+ cells, CD3+ CD4+ T cells, CD3+ CD4+ CD25+ T cells, CD3+ CD4+ CD25+ CD127low/negative Tregs and CD34+ cells and calculated cell yields, recruitment factors and collection efficiency for all cell types. We compared allogeneic data with autologous data.
Results
Autologous PBSC show significantly lower concentrations of T‐cell subsets compared to allogeneic PBSC (17 112/μl CD4+, 14 858/μl CD4+ CD25+ and 1579/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in autologous compared to 65 539/μl CD4+, 44 208+/μl CD4+ CD25+ and 5040/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in allogeneic PBSC, respectively), in contrast to CD34+ concentrations (5342/μl CD34+ in autologous compared to 2367/μl CD34+ in allogeneic PBSC, respectively). Accordantly, all T‐cell yields are lower in the autologous setting compared to allogeneic PBSC. However, recruitment factor and collection efficiency of all cell types are higher in autologous compared to allogeneic PBSC, but not all parameters differ significantly when groups are compared.
Conclusion
T‐cell subsets and especially Tregs are a substantial part of PBSC transplantation, as considerable recruitment during apheresis occurs. In large volume apheresis, the collection efficiency of Treg is comparable to that of CD34+ cells, while recruitment factors are even higher. |
| doi_str_mv | 10.1111/vox.12289 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1722925671</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3835696201</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3369-4504f295ebca40fc766d0e7e2c0168b30ebe369745aa6181b23fa3d8903307453</originalsourceid><addsrcrecordid>eNp1kE1P3DAURa0KVAbaRf9AZYlNWQSe7cSOlzDqUCToLAqUneU4L20gEw92wse_r4dhWCDVG8vWuVdXh5AvDA5ZOkcP_umQcV7qD2TCci4yyBlskQlAzjMNoHbIboy3AFDysvhIdriEHLSCCfl1mTnsOhrHKuIQadtTOw6-83_8GKnta2q79MAeW0eXGNrlXwy2o1XnfU3jgAv6kne-d9gPwQ4YP5HtxnYRP7_ee-Rq9v1y-iM7n5-eTY_PMyeE1FleQN5wXWDlbA6NU1LWgAq5AybLSgBWmDiVF9ZKVrKKi8aKutQgBKRfsUe-rXuXwd-PGAezaONqje0xrTdMca55IRVL6P479NaPoU_rVhTTSkulE3WwplzwMQZszDK0CxueDQOzMm2SafNiOrFfXxvHaoH1G7lRm4CjNfDYdvj8_yZzPb_ZVGbrRJu8Pr0lbLgzUglVmN8_T43gnN9ML2bmRPwDMhKVxQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1721979679</pqid></control><display><type>article</type><title>T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Strobel, J. ; Moellmer, I. ; Zingsem, J. ; Hauck-Dlimi, B. ; Eckstein, R. ; Strasser, E.</creator><creatorcontrib>Strobel, J. ; Moellmer, I. ; Zingsem, J. ; Hauck-Dlimi, B. ; Eckstein, R. ; Strasser, E.</creatorcontrib><description>Background and Objectives
Regulatory T cells (Tregs) and other T‐cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting.
Material and Methods
We characterized 53 donors, patients and peripheral blood stem cell concentrates (PBSC) regarding the content of CD45+ cells, lymphocytes, CD3+ cells, CD3+ CD4+ T cells, CD3+ CD4+ CD25+ T cells, CD3+ CD4+ CD25+ CD127low/negative Tregs and CD34+ cells and calculated cell yields, recruitment factors and collection efficiency for all cell types. We compared allogeneic data with autologous data.
Results
Autologous PBSC show significantly lower concentrations of T‐cell subsets compared to allogeneic PBSC (17 112/μl CD4+, 14 858/μl CD4+ CD25+ and 1579/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in autologous compared to 65 539/μl CD4+, 44 208+/μl CD4+ CD25+ and 5040/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in allogeneic PBSC, respectively), in contrast to CD34+ concentrations (5342/μl CD34+ in autologous compared to 2367/μl CD34+ in allogeneic PBSC, respectively). Accordantly, all T‐cell yields are lower in the autologous setting compared to allogeneic PBSC. However, recruitment factor and collection efficiency of all cell types are higher in autologous compared to allogeneic PBSC, but not all parameters differ significantly when groups are compared.
Conclusion
T‐cell subsets and especially Tregs are a substantial part of PBSC transplantation, as considerable recruitment during apheresis occurs. In large volume apheresis, the collection efficiency of Treg is comparable to that of CD34+ cells, while recruitment factors are even higher.</description><identifier>ISSN: 0042-9007</identifier><identifier>EISSN: 1423-0410</identifier><identifier>DOI: 10.1111/vox.12289</identifier><identifier>PMID: 26040970</identifier><identifier>CODEN: VOSAAD</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Aged ; apheresis ; Blood & organ donations ; Blood Transfusion, Autologous - adverse effects ; Child, Preschool ; Female ; Hematopoietic Stem Cell Transplantation - adverse effects ; Hematopoietic Stem Cell Transplantation - methods ; Hematopoietic Stem Cells - immunology ; Humans ; Infant ; Male ; Middle Aged ; peripheral blood stem cells ; regulatory T cells ; Stem cells ; T cell receptors ; T-cell subsets ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - transplantation ; Transplantation, Homologous - adverse effects ; Transplants & implants</subject><ispartof>Vox sanguinis, 2015-11, Vol.109 (4), p.375-386</ispartof><rights>2015 International Society of Blood Transfusion</rights><rights>2015 International Society of Blood Transfusion.</rights><rights>Copyright © 2015 International Society of Blood Transfusion</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3369-4504f295ebca40fc766d0e7e2c0168b30ebe369745aa6181b23fa3d8903307453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fvox.12289$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fvox.12289$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26040970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Strobel, J.</creatorcontrib><creatorcontrib>Moellmer, I.</creatorcontrib><creatorcontrib>Zingsem, J.</creatorcontrib><creatorcontrib>Hauck-Dlimi, B.</creatorcontrib><creatorcontrib>Eckstein, R.</creatorcontrib><creatorcontrib>Strasser, E.</creatorcontrib><title>T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates</title><title>Vox sanguinis</title><addtitle>Vox Sang</addtitle><description>Background and Objectives
Regulatory T cells (Tregs) and other T‐cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting.
Material and Methods
We characterized 53 donors, patients and peripheral blood stem cell concentrates (PBSC) regarding the content of CD45+ cells, lymphocytes, CD3+ cells, CD3+ CD4+ T cells, CD3+ CD4+ CD25+ T cells, CD3+ CD4+ CD25+ CD127low/negative Tregs and CD34+ cells and calculated cell yields, recruitment factors and collection efficiency for all cell types. We compared allogeneic data with autologous data.
Results
Autologous PBSC show significantly lower concentrations of T‐cell subsets compared to allogeneic PBSC (17 112/μl CD4+, 14 858/μl CD4+ CD25+ and 1579/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in autologous compared to 65 539/μl CD4+, 44 208+/μl CD4+ CD25+ and 5040/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in allogeneic PBSC, respectively), in contrast to CD34+ concentrations (5342/μl CD34+ in autologous compared to 2367/μl CD34+ in allogeneic PBSC, respectively). Accordantly, all T‐cell yields are lower in the autologous setting compared to allogeneic PBSC. However, recruitment factor and collection efficiency of all cell types are higher in autologous compared to allogeneic PBSC, but not all parameters differ significantly when groups are compared.
Conclusion
T‐cell subsets and especially Tregs are a substantial part of PBSC transplantation, as considerable recruitment during apheresis occurs. In large volume apheresis, the collection efficiency of Treg is comparable to that of CD34+ cells, while recruitment factors are even higher.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>apheresis</subject><subject>Blood & organ donations</subject><subject>Blood Transfusion, Autologous - adverse effects</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Hematopoietic Stem Cell Transplantation - adverse effects</subject><subject>Hematopoietic Stem Cell Transplantation - methods</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Middle Aged</subject><subject>peripheral blood stem cells</subject><subject>regulatory T cells</subject><subject>Stem cells</subject><subject>T cell receptors</subject><subject>T-cell subsets</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - transplantation</subject><subject>Transplantation, Homologous - adverse effects</subject><subject>Transplants & implants</subject><issn>0042-9007</issn><issn>1423-0410</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1P3DAURa0KVAbaRf9AZYlNWQSe7cSOlzDqUCToLAqUneU4L20gEw92wse_r4dhWCDVG8vWuVdXh5AvDA5ZOkcP_umQcV7qD2TCci4yyBlskQlAzjMNoHbIboy3AFDysvhIdriEHLSCCfl1mTnsOhrHKuIQadtTOw6-83_8GKnta2q79MAeW0eXGNrlXwy2o1XnfU3jgAv6kne-d9gPwQ4YP5HtxnYRP7_ee-Rq9v1y-iM7n5-eTY_PMyeE1FleQN5wXWDlbA6NU1LWgAq5AybLSgBWmDiVF9ZKVrKKi8aKutQgBKRfsUe-rXuXwd-PGAezaONqje0xrTdMca55IRVL6P479NaPoU_rVhTTSkulE3WwplzwMQZszDK0CxueDQOzMm2SafNiOrFfXxvHaoH1G7lRm4CjNfDYdvj8_yZzPb_ZVGbrRJu8Pr0lbLgzUglVmN8_T43gnN9ML2bmRPwDMhKVxQ</recordid><startdate>201511</startdate><enddate>201511</enddate><creator>Strobel, J.</creator><creator>Moellmer, I.</creator><creator>Zingsem, J.</creator><creator>Hauck-Dlimi, B.</creator><creator>Eckstein, R.</creator><creator>Strasser, E.</creator><general>Blackwell Publishing Ltd</general><general>S. 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Regulatory T cells (Tregs) and other T‐cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting.
Material and Methods
We characterized 53 donors, patients and peripheral blood stem cell concentrates (PBSC) regarding the content of CD45+ cells, lymphocytes, CD3+ cells, CD3+ CD4+ T cells, CD3+ CD4+ CD25+ T cells, CD3+ CD4+ CD25+ CD127low/negative Tregs and CD34+ cells and calculated cell yields, recruitment factors and collection efficiency for all cell types. We compared allogeneic data with autologous data.
Results
Autologous PBSC show significantly lower concentrations of T‐cell subsets compared to allogeneic PBSC (17 112/μl CD4+, 14 858/μl CD4+ CD25+ and 1579/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in autologous compared to 65 539/μl CD4+, 44 208+/μl CD4+ CD25+ and 5040/μl CD3+ CD4+ CD25+ CD127low/negative Tregs in allogeneic PBSC, respectively), in contrast to CD34+ concentrations (5342/μl CD34+ in autologous compared to 2367/μl CD34+ in allogeneic PBSC, respectively). Accordantly, all T‐cell yields are lower in the autologous setting compared to allogeneic PBSC. However, recruitment factor and collection efficiency of all cell types are higher in autologous compared to allogeneic PBSC, but not all parameters differ significantly when groups are compared.
Conclusion
T‐cell subsets and especially Tregs are a substantial part of PBSC transplantation, as considerable recruitment during apheresis occurs. In large volume apheresis, the collection efficiency of Treg is comparable to that of CD34+ cells, while recruitment factors are even higher.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26040970</pmid><doi>10.1111/vox.12289</doi><tpages>12</tpages></addata></record> |
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| ispartof | Vox sanguinis, 2015-11, Vol.109 (4), p.375-386 |
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| subjects | Adolescent Adult Aged apheresis Blood & organ donations Blood Transfusion, Autologous - adverse effects Child, Preschool Female Hematopoietic Stem Cell Transplantation - adverse effects Hematopoietic Stem Cell Transplantation - methods Hematopoietic Stem Cells - immunology Humans Infant Male Middle Aged peripheral blood stem cells regulatory T cells Stem cells T cell receptors T-cell subsets T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - transplantation Transplantation, Homologous - adverse effects Transplants & implants |
| title | T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates |
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