Role of CGRP modified mesenchymal stem cells in restenosis in rat model with carotid angioplasty
To explore the role of calcitonin gene-related peptide (CGRP) in the proliferation of vascular smooth muscle cells (VSMCs) and restenosis. The high-expression CGRP lentivirus [Lenti-green fluorescent protein (GFP)-CGRP] was constructed. And mesenchymal stem cells (MSCs) were transfected with Lenti-G...
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| Veröffentlicht in: | Zhong hua yi xue za zhi 2015-05, Vol.95 (20), p.1619-1624 |
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| container_title | Zhong hua yi xue za zhi |
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| creator | Shi, Bei Chen, Panke Long, Xianping Zhao, Ranzun Deng, Wenwen |
| description | To explore the role of calcitonin gene-related peptide (CGRP) in the proliferation of vascular smooth muscle cells (VSMCs) and restenosis.
The high-expression CGRP lentivirus [Lenti-green fluorescent protein (GFP)-CGRP] was constructed. And mesenchymal stem cells (MSCs) were transfected with Lenti-GFP-CGRP, Lenti-GFP and phosphate buffer saline (PBS). Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to determine the CGRP gene and protein expression level of smooth muscle cells in each group respectively. Then MSCs were co-cultured with VSMCs. Experimental groups were MSCs(CGRP+/+)+VSMCs, MSCs(CGRP-/-)+VSMCs and MSCs(PBS)+VSMCs groups. The method of methyl thiazolyl tetrazolium (MTT) was employed to detect the proliferation of smooth muscle cells. Sacculus damaged atherosclerotic carotid was prepared according to the previous study. MSCs were transfected with Lv-CGRP-EGFP and Lv-CGRP and then transplanted into rat model. At Day 7 post-transplantat |
| doi_str_mv | 10.3760/cma.j.issn.0376-2491.2015.20.018 |
| format | Article |
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The high-expression CGRP lentivirus [Lenti-green fluorescent protein (GFP)-CGRP] was constructed. And mesenchymal stem cells (MSCs) were transfected with Lenti-GFP-CGRP, Lenti-GFP and phosphate buffer saline (PBS). Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to determine the CGRP gene and protein expression level of smooth muscle cells in each group respectively. Then MSCs were co-cultured with VSMCs. Experimental groups were MSCs(CGRP+/+)+VSMCs, MSCs(CGRP-/-)+VSMCs and MSCs(PBS)+VSMCs groups. The method of methyl thiazolyl tetrazolium (MTT) was employed to detect the proliferation of smooth muscle cells. Sacculus damaged atherosclerotic carotid was prepared according to the previous study. MSCs were transfected with Lv-CGRP-EGFP and Lv-CGRP and then transplanted into rat model. At Day 7 post-transplantat</description><identifier>ISSN: 0376-2491</identifier><identifier>DOI: 10.3760/cma.j.issn.0376-2491.2015.20.018</identifier><identifier>PMID: 26463616</identifier><language>chi</language><publisher>China</publisher><subject>Angioplasty ; Animals ; Calcitonin Gene-Related Peptide ; Carotid Arteries ; Carotid Artery, Common ; Coculture Techniques ; Endothelium, Vascular ; Enzyme-Linked Immunosorbent Assay ; Green Fluorescent Proteins ; Lentivirus ; Mesenchymal Stromal Cells ; Muscle, Smooth, Vascular ; Myocytes, Smooth Muscle ; Proliferating Cell Nuclear Antigen ; Rats ; Transfection</subject><ispartof>Zhong hua yi xue za zhi, 2015-05, Vol.95 (20), p.1619-1624</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26463616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Bei</creatorcontrib><creatorcontrib>Chen, Panke</creatorcontrib><creatorcontrib>Long, Xianping</creatorcontrib><creatorcontrib>Zhao, Ranzun</creatorcontrib><creatorcontrib>Deng, Wenwen</creatorcontrib><title>Role of CGRP modified mesenchymal stem cells in restenosis in rat model with carotid angioplasty</title><title>Zhong hua yi xue za zhi</title><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><description>To explore the role of calcitonin gene-related peptide (CGRP) in the proliferation of vascular smooth muscle cells (VSMCs) and restenosis.
The high-expression CGRP lentivirus [Lenti-green fluorescent protein (GFP)-CGRP] was constructed. And mesenchymal stem cells (MSCs) were transfected with Lenti-GFP-CGRP, Lenti-GFP and phosphate buffer saline (PBS). Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to determine the CGRP gene and protein expression level of smooth muscle cells in each group respectively. Then MSCs were co-cultured with VSMCs. Experimental groups were MSCs(CGRP+/+)+VSMCs, MSCs(CGRP-/-)+VSMCs and MSCs(PBS)+VSMCs groups. The method of methyl thiazolyl tetrazolium (MTT) was employed to detect the proliferation of smooth muscle cells. Sacculus damaged atherosclerotic carotid was prepared according to the previous study. MSCs were transfected with Lv-CGRP-EGFP and Lv-CGRP and then transplanted into rat model. At Day 7 post-transplantat</description><subject>Angioplasty</subject><subject>Animals</subject><subject>Calcitonin Gene-Related Peptide</subject><subject>Carotid Arteries</subject><subject>Carotid Artery, Common</subject><subject>Coculture Techniques</subject><subject>Endothelium, Vascular</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Green Fluorescent Proteins</subject><subject>Lentivirus</subject><subject>Mesenchymal Stromal Cells</subject><subject>Muscle, Smooth, Vascular</subject><subject>Myocytes, Smooth Muscle</subject><subject>Proliferating Cell Nuclear Antigen</subject><subject>Rats</subject><subject>Transfection</subject><issn>0376-2491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1OwzAQhH0A0Qr6CsjHXhL8Ezv2EVVQkCqBKjgHx1lTozgOcSrUtydVC5ddzejb1WgQWlKS81KSOxtM_pX7lLqcTEbGCk1zRqiYRk6oukDzf3-GFin5mhQl14wweoVmTBaSSyrn6GMbW8DR4dV6-4pDbLzz0OAACTq7OwTT4jRCwBbaNmHf4QEm3cXkT8qMxyNo8Y8fd9iaIY6-wab79LFvTRoPN-jSmTbB4ryv0fvjw9vqKdu8rJ9X95usp1qOmTBEKSbAccEJ5bYxWktaW4C6qJ0GyhXhxk6UEJzrptRKaCcVdUSDcZZfo-Xpbz_E7_0Usgo-HVObDuI-VbRkrKBKlXRCb8_ovg7QVP3ggxkO1V8r_BepFWgx</recordid><startdate>20150526</startdate><enddate>20150526</enddate><creator>Shi, Bei</creator><creator>Chen, Panke</creator><creator>Long, Xianping</creator><creator>Zhao, Ranzun</creator><creator>Deng, Wenwen</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20150526</creationdate><title>Role of CGRP modified mesenchymal stem cells in restenosis in rat model with carotid angioplasty</title><author>Shi, Bei ; Chen, Panke ; Long, Xianping ; Zhao, Ranzun ; Deng, Wenwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p196t-5a08825ef353013cda9961bceeb4bf9e13803aca0855339d79859f681f09eafc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2015</creationdate><topic>Angioplasty</topic><topic>Animals</topic><topic>Calcitonin Gene-Related Peptide</topic><topic>Carotid Arteries</topic><topic>Carotid Artery, Common</topic><topic>Coculture Techniques</topic><topic>Endothelium, Vascular</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Green Fluorescent Proteins</topic><topic>Lentivirus</topic><topic>Mesenchymal Stromal Cells</topic><topic>Muscle, Smooth, Vascular</topic><topic>Myocytes, Smooth Muscle</topic><topic>Proliferating Cell Nuclear Antigen</topic><topic>Rats</topic><topic>Transfection</topic><toplevel>online_resources</toplevel><creatorcontrib>Shi, Bei</creatorcontrib><creatorcontrib>Chen, Panke</creatorcontrib><creatorcontrib>Long, Xianping</creatorcontrib><creatorcontrib>Zhao, Ranzun</creatorcontrib><creatorcontrib>Deng, Wenwen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong hua yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Bei</au><au>Chen, Panke</au><au>Long, Xianping</au><au>Zhao, Ranzun</au><au>Deng, Wenwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of CGRP modified mesenchymal stem cells in restenosis in rat model with carotid angioplasty</atitle><jtitle>Zhong hua yi xue za zhi</jtitle><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><date>2015-05-26</date><risdate>2015</risdate><volume>95</volume><issue>20</issue><spage>1619</spage><epage>1624</epage><pages>1619-1624</pages><issn>0376-2491</issn><abstract>To explore the role of calcitonin gene-related peptide (CGRP) in the proliferation of vascular smooth muscle cells (VSMCs) and restenosis.
The high-expression CGRP lentivirus [Lenti-green fluorescent protein (GFP)-CGRP] was constructed. And mesenchymal stem cells (MSCs) were transfected with Lenti-GFP-CGRP, Lenti-GFP and phosphate buffer saline (PBS). Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to determine the CGRP gene and protein expression level of smooth muscle cells in each group respectively. Then MSCs were co-cultured with VSMCs. Experimental groups were MSCs(CGRP+/+)+VSMCs, MSCs(CGRP-/-)+VSMCs and MSCs(PBS)+VSMCs groups. The method of methyl thiazolyl tetrazolium (MTT) was employed to detect the proliferation of smooth muscle cells. Sacculus damaged atherosclerotic carotid was prepared according to the previous study. MSCs were transfected with Lv-CGRP-EGFP and Lv-CGRP and then transplanted into rat model. At Day 7 post-transplantat</abstract><cop>China</cop><pmid>26463616</pmid><doi>10.3760/cma.j.issn.0376-2491.2015.20.018</doi><tpages>6</tpages></addata></record> |
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| ispartof | Zhong hua yi xue za zhi, 2015-05, Vol.95 (20), p.1619-1624 |
| issn | 0376-2491 |
| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Angioplasty Animals Calcitonin Gene-Related Peptide Carotid Arteries Carotid Artery, Common Coculture Techniques Endothelium, Vascular Enzyme-Linked Immunosorbent Assay Green Fluorescent Proteins Lentivirus Mesenchymal Stromal Cells Muscle, Smooth, Vascular Myocytes, Smooth Muscle Proliferating Cell Nuclear Antigen Rats Transfection |
| title | Role of CGRP modified mesenchymal stem cells in restenosis in rat model with carotid angioplasty |
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