Characterization of Listeria monocytogenes from an ice cream plant by serotyping and pulsed-field gel electrophoresis
One dominating strain of serotype 1/2b was found when serotyping and pulsed-field gel electrophoresis (PFGE) patterns were used for the characterization of 41 Listeria monocytogenes isolates originating from an ice cream plant. Samples were taken from the production environment, equipment and ice cr...
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| Veröffentlicht in: | International journal of food microbiology 1999-02, Vol.46 (3), p.187-192 |
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| container_title | International journal of food microbiology |
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| creator | Miettinen, Maria K Björkroth, K.Johanna Korkeala, Hannu J |
| description | One dominating strain of serotype 1/2b was found when serotyping and pulsed-field gel electrophoresis (PFGE) patterns were used for the characterization of 41
Listeria monocytogenes isolates originating from an ice cream plant. Samples were taken from the production environment, equipment and ice cream during the years 1990–1997. Serotyping divided the isolates into two serovars, 1/2b and 4b. Three rare-cutting enzymes (
ApaI,
AscI and
SmaI) were used in the creation of PFGE patterns.
AscI resulted in the best restriction enzyme digestion patterns (REDPs) for visual comparison. Eight different
AscI REDPs were obtained, whereas
ApaI produced six and
SmaI seven banding patterns. When one-band differences are taken into account, 12 different PFGE types were distinguished based on information obtained with all three enzymes. The dominant PFGE type was found to have persisted in the ice cream plant for seven years. Improved and precisely targeted cleaning and disinfection practices combined with structural changes making for easier cleaning of the packaging machine, resulted in eradication of
L. monocytogenes from this plant. |
| doi_str_mv | 10.1016/S0168-1605(98)00185-8 |
| format | Article |
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Listeria monocytogenes isolates originating from an ice cream plant. Samples were taken from the production environment, equipment and ice cream during the years 1990–1997. Serotyping divided the isolates into two serovars, 1/2b and 4b. Three rare-cutting enzymes (
ApaI,
AscI and
SmaI) were used in the creation of PFGE patterns.
AscI resulted in the best restriction enzyme digestion patterns (REDPs) for visual comparison. Eight different
AscI REDPs were obtained, whereas
ApaI produced six and
SmaI seven banding patterns. When one-band differences are taken into account, 12 different PFGE types were distinguished based on information obtained with all three enzymes. The dominant PFGE type was found to have persisted in the ice cream plant for seven years. Improved and precisely targeted cleaning and disinfection practices combined with structural changes making for easier cleaning of the packaging machine, resulted in eradication of
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Listeria monocytogenes isolates originating from an ice cream plant. Samples were taken from the production environment, equipment and ice cream during the years 1990–1997. Serotyping divided the isolates into two serovars, 1/2b and 4b. Three rare-cutting enzymes (
ApaI,
AscI and
SmaI) were used in the creation of PFGE patterns.
AscI resulted in the best restriction enzyme digestion patterns (REDPs) for visual comparison. Eight different
AscI REDPs were obtained, whereas
ApaI produced six and
SmaI seven banding patterns. When one-band differences are taken into account, 12 different PFGE types were distinguished based on information obtained with all three enzymes. The dominant PFGE type was found to have persisted in the ice cream plant for seven years. Improved and precisely targeted cleaning and disinfection practices combined with structural changes making for easier cleaning of the packaging machine, resulted in eradication of
L. monocytogenes from this plant.</description><subject>Biological and medical sciences</subject><subject>DNA Restriction Enzymes - chemistry</subject><subject>DNA, Bacterial - chemistry</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Food-Processing Industry - standards</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ice Cream - microbiology</subject><subject>Ice cream plant</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - chemistry</subject><subject>Listeria monocytogenes - classification</subject><subject>Listeriosis - prevention & control</subject><subject>Milk and cheese industries. 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Psychology</topic><topic>Ice Cream - microbiology</topic><topic>Ice cream plant</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - chemistry</topic><topic>Listeria monocytogenes - classification</topic><topic>Listeriosis - prevention & control</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Pulsed-field gel electrophoresis</topic><topic>Serotyping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miettinen, Maria K</creatorcontrib><creatorcontrib>Björkroth, K.Johanna</creatorcontrib><creatorcontrib>Korkeala, Hannu J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miettinen, Maria K</au><au>Björkroth, K.Johanna</au><au>Korkeala, Hannu J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Listeria monocytogenes from an ice cream plant by serotyping and pulsed-field gel electrophoresis</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>1999-02-18</date><risdate>1999</risdate><volume>46</volume><issue>3</issue><spage>187</spage><epage>192</epage><pages>187-192</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>One dominating strain of serotype 1/2b was found when serotyping and pulsed-field gel electrophoresis (PFGE) patterns were used for the characterization of 41
Listeria monocytogenes isolates originating from an ice cream plant. Samples were taken from the production environment, equipment and ice cream during the years 1990–1997. Serotyping divided the isolates into two serovars, 1/2b and 4b. Three rare-cutting enzymes (
ApaI,
AscI and
SmaI) were used in the creation of PFGE patterns.
AscI resulted in the best restriction enzyme digestion patterns (REDPs) for visual comparison. Eight different
AscI REDPs were obtained, whereas
ApaI produced six and
SmaI seven banding patterns. When one-band differences are taken into account, 12 different PFGE types were distinguished based on information obtained with all three enzymes. The dominant PFGE type was found to have persisted in the ice cream plant for seven years. Improved and precisely targeted cleaning and disinfection practices combined with structural changes making for easier cleaning of the packaging machine, resulted in eradication of
L. monocytogenes from this plant.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10100898</pmid><doi>10.1016/S0168-1605(98)00185-8</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences DNA Restriction Enzymes - chemistry DNA, Bacterial - chemistry Electrophoresis, Gel, Pulsed-Field Food industries Food Microbiology Food-Processing Industry - standards Fundamental and applied biological sciences. Psychology Ice Cream - microbiology Ice cream plant Listeria monocytogenes Listeria monocytogenes - chemistry Listeria monocytogenes - classification Listeriosis - prevention & control Milk and cheese industries. Ice creams Pulsed-field gel electrophoresis Serotyping |
| title | Characterization of Listeria monocytogenes from an ice cream plant by serotyping and pulsed-field gel electrophoresis |
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