Decreased UBASH3A mRNA Expression Levels in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus
Increasing evidence has demonstrated the association between UBASH3A gene and multiple autoimmune diseases (ADs). The aim of our study was to explore the potential effect of UBASH3A messenger RNA (mRNA) expression and its role in the pathogenesis of systemic lupus erythematosus (SLE). UBASH3A mRNA l...
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| Veröffentlicht in: | Inflammation 2015-10, Vol.38 (5), p.1903-1910 |
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| creator | Liu, Jie Ni, Jing Li, Lian-Ju Leng, Rui-Xue Pan, Hai-Feng Ye, Dong-Qing |
| description | Increasing evidence has demonstrated the association between UBASH3A gene and multiple autoimmune diseases (ADs). The aim of our study was to explore the potential effect of UBASH3A messenger RNA (mRNA) expression and its role in the pathogenesis of systemic lupus erythematosus (SLE). UBASH3A mRNA levels were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) in total RNA, isolated from the peripheral blood mononuclear cells (PBMCs) of 32 SLE patients and 30 healthy donors with TRIzol Reagent. The expression level of UBASH3A mRNA was significantly reduced in PBMCs from SLE patients when compared with healthy controls (
p
= 0.002). UBASH3A mRNA expression levels in lower active SLE were significantly lower than that in inactive SLE groups (
p
= 0.000). There was a negative association between mRNA levels of hyper-active and lower-active SLE patients (
p
= 0.000). Moreover, a significant negative correlation between UBASH3A mRNA expression and the onset age of SLE patients was found (
p
= 0.044). A negative correlation was found between UBASH3A mRNA expression and SLEDAI (
p
= 0.049). Nevertheless, no significant difference was found between patients with lupus nephritis (LN) and those without LN (
p
= 0.392). The presence of leukopenia, positive for anti-dsDNA antibody and anti-SSB antibody were associated with UBASH3A mRNA levels in SLE patients (all
p
|
| doi_str_mv | 10.1007/s10753-015-0170-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1722184058</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1709707338</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-4708adcc665924fd2384a2b6210e9dd5d70f8eedd5cdad954670c02f55238b663</originalsourceid><addsrcrecordid>eNqNkcFu1DAQhi0EotvCA3BBlrhwCYztOE6O22VLkRaoKD1HXnvCpkri4EmAvfDseNmCEBISB2ss-Zt_PPoYeyLghQAwL0mA0SoDodMxkFX32EJoozKpTXGfLUAVkKmqMifslOgWAMqqVA_ZidSlKYyQC_b9FbqIltDzm_Pl9aVa8v7DuyVffxsjErVh4Bv8gh3xduBXGNtxh9F2_LwLwfO3YQjD7Dq0ka-wS1QTQ8-v7NTiMBH_2k47fr2nCfvW8c08zsTXcT_tsLdToJkesQeN7Qgf39UzdnOx_ri6zDbvX79ZLTeZy42estxAab1zRaErmTdeqjK3cltIAVh5r72BpkRMN-etr3ReGHAgG60TuS0KdcaeH3PHGD7PSFPdt-TSj-2AYaZaGClFmYMu_wOFyoBR6oA--wu9DXMc0iI_KS20hjxR4ki5GIgiNvUY297GfS2gPnisjx7r5LE-eKyr1PP0Lnne9uh_d_wSlwB5BCg9DZ8w_jH6n6k_AOIDp60</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1709515504</pqid></control><display><type>article</type><title>Decreased UBASH3A mRNA Expression Levels in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Liu, Jie ; Ni, Jing ; Li, Lian-Ju ; Leng, Rui-Xue ; Pan, Hai-Feng ; Ye, Dong-Qing</creator><creatorcontrib>Liu, Jie ; Ni, Jing ; Li, Lian-Ju ; Leng, Rui-Xue ; Pan, Hai-Feng ; Ye, Dong-Qing</creatorcontrib><description>Increasing evidence has demonstrated the association between UBASH3A gene and multiple autoimmune diseases (ADs). The aim of our study was to explore the potential effect of UBASH3A messenger RNA (mRNA) expression and its role in the pathogenesis of systemic lupus erythematosus (SLE). UBASH3A mRNA levels were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) in total RNA, isolated from the peripheral blood mononuclear cells (PBMCs) of 32 SLE patients and 30 healthy donors with TRIzol Reagent. The expression level of UBASH3A mRNA was significantly reduced in PBMCs from SLE patients when compared with healthy controls (
p
= 0.002). UBASH3A mRNA expression levels in lower active SLE were significantly lower than that in inactive SLE groups (
p
= 0.000). There was a negative association between mRNA levels of hyper-active and lower-active SLE patients (
p
= 0.000). Moreover, a significant negative correlation between UBASH3A mRNA expression and the onset age of SLE patients was found (
p
= 0.044). A negative correlation was found between UBASH3A mRNA expression and SLEDAI (
p
= 0.049). Nevertheless, no significant difference was found between patients with lupus nephritis (LN) and those without LN (
p
= 0.392). The presence of leukopenia, positive for anti-dsDNA antibody and anti-SSB antibody were associated with UBASH3A mRNA levels in SLE patients (all
p
< 0.05). The dysregulation of UBASH3A mRNA levels in SLE patients and their correlations with experimental parameters suggested that UBASH3A may involve in the pathogenesis of SLE.</description><identifier>ISSN: 0360-3997</identifier><identifier>EISSN: 1573-2576</identifier><identifier>DOI: 10.1007/s10753-015-0170-9</identifier><identifier>PMID: 25876712</identifier><identifier>CODEN: INFLD4</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adaptor Proteins, Signal Transducing - biosynthesis ; Adaptor Proteins, Signal Transducing - genetics ; Adult ; Biomedical and Life Sciences ; Biomedicine ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Immunology ; Internal Medicine ; Leukocytes, Mononuclear - metabolism ; Leukocytes, Mononuclear - pathology ; Lupus Erythematosus, Systemic - diagnosis ; Lupus Erythematosus, Systemic - genetics ; Lupus Erythematosus, Systemic - metabolism ; Male ; Middle Aged ; Pathology ; Pharmacology/Toxicology ; Rheumatology ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Young Adult</subject><ispartof>Inflammation, 2015-10, Vol.38 (5), p.1903-1910</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-4708adcc665924fd2384a2b6210e9dd5d70f8eedd5cdad954670c02f55238b663</citedby><cites>FETCH-LOGICAL-c475t-4708adcc665924fd2384a2b6210e9dd5d70f8eedd5cdad954670c02f55238b663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10753-015-0170-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10753-015-0170-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25876712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>Ni, Jing</creatorcontrib><creatorcontrib>Li, Lian-Ju</creatorcontrib><creatorcontrib>Leng, Rui-Xue</creatorcontrib><creatorcontrib>Pan, Hai-Feng</creatorcontrib><creatorcontrib>Ye, Dong-Qing</creatorcontrib><title>Decreased UBASH3A mRNA Expression Levels in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus</title><title>Inflammation</title><addtitle>Inflammation</addtitle><addtitle>Inflammation</addtitle><description>Increasing evidence has demonstrated the association between UBASH3A gene and multiple autoimmune diseases (ADs). The aim of our study was to explore the potential effect of UBASH3A messenger RNA (mRNA) expression and its role in the pathogenesis of systemic lupus erythematosus (SLE). UBASH3A mRNA levels were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) in total RNA, isolated from the peripheral blood mononuclear cells (PBMCs) of 32 SLE patients and 30 healthy donors with TRIzol Reagent. The expression level of UBASH3A mRNA was significantly reduced in PBMCs from SLE patients when compared with healthy controls (
p
= 0.002). UBASH3A mRNA expression levels in lower active SLE were significantly lower than that in inactive SLE groups (
p
= 0.000). There was a negative association between mRNA levels of hyper-active and lower-active SLE patients (
p
= 0.000). Moreover, a significant negative correlation between UBASH3A mRNA expression and the onset age of SLE patients was found (
p
= 0.044). A negative correlation was found between UBASH3A mRNA expression and SLEDAI (
p
= 0.049). Nevertheless, no significant difference was found between patients with lupus nephritis (LN) and those without LN (
p
= 0.392). The presence of leukopenia, positive for anti-dsDNA antibody and anti-SSB antibody were associated with UBASH3A mRNA levels in SLE patients (all
p
< 0.05). The dysregulation of UBASH3A mRNA levels in SLE patients and their correlations with experimental parameters suggested that UBASH3A may involve in the pathogenesis of SLE.</description><subject>Adaptor Proteins, Signal Transducing - biosynthesis</subject><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Adult</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunology</subject><subject>Internal Medicine</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Leukocytes, Mononuclear - pathology</subject><subject>Lupus Erythematosus, Systemic - diagnosis</subject><subject>Lupus Erythematosus, Systemic - genetics</subject><subject>Lupus Erythematosus, Systemic - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pathology</subject><subject>Pharmacology/Toxicology</subject><subject>Rheumatology</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Young Adult</subject><issn>0360-3997</issn><issn>1573-2576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkcFu1DAQhi0EotvCA3BBlrhwCYztOE6O22VLkRaoKD1HXnvCpkri4EmAvfDseNmCEBISB2ss-Zt_PPoYeyLghQAwL0mA0SoDodMxkFX32EJoozKpTXGfLUAVkKmqMifslOgWAMqqVA_ZidSlKYyQC_b9FbqIltDzm_Pl9aVa8v7DuyVffxsjErVh4Bv8gh3xduBXGNtxh9F2_LwLwfO3YQjD7Dq0ka-wS1QTQ8-v7NTiMBH_2k47fr2nCfvW8c08zsTXcT_tsLdToJkesQeN7Qgf39UzdnOx_ri6zDbvX79ZLTeZy42estxAab1zRaErmTdeqjK3cltIAVh5r72BpkRMN-etr3ReGHAgG60TuS0KdcaeH3PHGD7PSFPdt-TSj-2AYaZaGClFmYMu_wOFyoBR6oA--wu9DXMc0iI_KS20hjxR4ki5GIgiNvUY297GfS2gPnisjx7r5LE-eKyr1PP0Lnne9uh_d_wSlwB5BCg9DZ8w_jH6n6k_AOIDp60</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Liu, Jie</creator><creator>Ni, Jing</creator><creator>Li, Lian-Ju</creator><creator>Leng, Rui-Xue</creator><creator>Pan, Hai-Feng</creator><creator>Ye, Dong-Qing</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20151001</creationdate><title>Decreased UBASH3A mRNA Expression Levels in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus</title><author>Liu, Jie ; Ni, Jing ; Li, Lian-Ju ; Leng, Rui-Xue ; Pan, Hai-Feng ; Ye, Dong-Qing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-4708adcc665924fd2384a2b6210e9dd5d70f8eedd5cdad954670c02f55238b663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adaptor Proteins, Signal Transducing - biosynthesis</topic><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Adult</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunology</topic><topic>Internal Medicine</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Leukocytes, Mononuclear - pathology</topic><topic>Lupus Erythematosus, Systemic - diagnosis</topic><topic>Lupus Erythematosus, Systemic - genetics</topic><topic>Lupus Erythematosus, Systemic - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pathology</topic><topic>Pharmacology/Toxicology</topic><topic>Rheumatology</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>Ni, Jing</creatorcontrib><creatorcontrib>Li, Lian-Ju</creatorcontrib><creatorcontrib>Leng, Rui-Xue</creatorcontrib><creatorcontrib>Pan, Hai-Feng</creatorcontrib><creatorcontrib>Ye, Dong-Qing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Inflammation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Jie</au><au>Ni, Jing</au><au>Li, Lian-Ju</au><au>Leng, Rui-Xue</au><au>Pan, Hai-Feng</au><au>Ye, Dong-Qing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased UBASH3A mRNA Expression Levels in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus</atitle><jtitle>Inflammation</jtitle><stitle>Inflammation</stitle><addtitle>Inflammation</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>38</volume><issue>5</issue><spage>1903</spage><epage>1910</epage><pages>1903-1910</pages><issn>0360-3997</issn><eissn>1573-2576</eissn><coden>INFLD4</coden><abstract>Increasing evidence has demonstrated the association between UBASH3A gene and multiple autoimmune diseases (ADs). The aim of our study was to explore the potential effect of UBASH3A messenger RNA (mRNA) expression and its role in the pathogenesis of systemic lupus erythematosus (SLE). UBASH3A mRNA levels were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) in total RNA, isolated from the peripheral blood mononuclear cells (PBMCs) of 32 SLE patients and 30 healthy donors with TRIzol Reagent. The expression level of UBASH3A mRNA was significantly reduced in PBMCs from SLE patients when compared with healthy controls (
p
= 0.002). UBASH3A mRNA expression levels in lower active SLE were significantly lower than that in inactive SLE groups (
p
= 0.000). There was a negative association between mRNA levels of hyper-active and lower-active SLE patients (
p
= 0.000). Moreover, a significant negative correlation between UBASH3A mRNA expression and the onset age of SLE patients was found (
p
= 0.044). A negative correlation was found between UBASH3A mRNA expression and SLEDAI (
p
= 0.049). Nevertheless, no significant difference was found between patients with lupus nephritis (LN) and those without LN (
p
= 0.392). The presence of leukopenia, positive for anti-dsDNA antibody and anti-SSB antibody were associated with UBASH3A mRNA levels in SLE patients (all
p
< 0.05). The dysregulation of UBASH3A mRNA levels in SLE patients and their correlations with experimental parameters suggested that UBASH3A may involve in the pathogenesis of SLE.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25876712</pmid><doi>10.1007/s10753-015-0170-9</doi><tpages>8</tpages></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - biosynthesis Adaptor Proteins, Signal Transducing - genetics Adult Biomedical and Life Sciences Biomedicine Female Gene Expression Regulation, Neoplastic Humans Immunology Internal Medicine Leukocytes, Mononuclear - metabolism Leukocytes, Mononuclear - pathology Lupus Erythematosus, Systemic - diagnosis Lupus Erythematosus, Systemic - genetics Lupus Erythematosus, Systemic - metabolism Male Middle Aged Pathology Pharmacology/Toxicology Rheumatology RNA, Messenger - biosynthesis RNA, Messenger - genetics Young Adult |
| title | Decreased UBASH3A mRNA Expression Levels in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus |
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