Combined Fluorescence and Magnetic Resonance Imaging of Primary Macrophage Migration to Sites of Acute Inflammation Using Near-Infrared Fluorescent Magnetic Nanoparticles
Purpose This study aimed to track the migration of primary macrophages labeled with near-infrared (NIR) fluorescent magnetic nanoparticles toward chemically induced acute inflammatory lesions in mice and to visualize the effect of anti-inflammatory drugs on macrophage migration using combined fluore...
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| Veröffentlicht in: | Molecular imaging and biology 2015-10, Vol.17 (5), p.643-651 |
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| creator | Kang, Sungmin Lee, Ho Won Jeon, Young Hyun Singh, Thoudam Debraj Choi, Yun Ju Park, Ji Young Kim, Jun Sung Lee, Hyunseung Hong, Kwan Soo Lee, Inkyu Jeong, Shin Young Lee, Sang-Woo Ha, Jeoung-Hee Ahn, Byeong-Cheol Lee, Jaetae |
| description | Purpose
This study aimed to track the migration of primary macrophages labeled with near-infrared (NIR) fluorescent magnetic nanoparticles toward chemically induced acute inflammatory lesions in mice and to visualize the effect of anti-inflammatory drugs on macrophage migration using combined fluorescence and magnetic resonance imaging (FLI/MRI).
Procedures
Primary macrophages were labeled with NIR fluorescent magnetic nanoparticles, and labeled cells were injected into mice intravenously. One day later, inflammation was induced by subcutaneous injection of 1 % carrageenan (CG) solution to footpads of the right hind leg, and phosphate-buffered saline (PBS) as control treatment was subcutaneously injected to footpad of the left hind leg. To evaluate the effect of drug treatment on macrophage migration, a single dose of dexamethasone (DEX) was intraperitoneally administered to the mice immediately after the induction of inflammation and was followed by combined FLI/MRI at predetermined time points.
Results
No difference in cellular viability or phagocytic activity was observed between the labeled and parent macrophages.
In vivo
optical imaging revealed an increase in FLI signals in CG-injected footpads in a time-dependent manner, but not in PBS-treated footpads. DEX treatment inhibited the migration of the labeled macrophages to the CG-injected footpads, with relative decreases in FLI activity. In accordance with FLI, T
2
*-weighted MR images showed hypo-intense signals in the CG-injected footpads but not in the PBS-injected footpads. The DEX-treated mice did not show a dark signal loss zone on MR images in the CG-treated paw.
Conclusions
We successfully tracked the migration of macrophages to inflammatory lesions using both FLI and MRI with NIR fluorescent magnetic nanoparticles and demonstrated the inhibitory effects of DEX on macrophage migration to inflammation sites. |
| doi_str_mv | 10.1007/s11307-015-0830-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1722183833</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1713530851</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-77b0ca72499524eb72485c062d92bcd9a1872b14897fbf0217f57a8f1833ae133</originalsourceid><addsrcrecordid>eNqNkc1u1TAQhS0EoqXwAGyQJTZsTD12HCfL6opCpbZUQNeW40xCqsS-2MmCPhJPiaOUnyIhdeXRzDdnxnMIeQn8LXCujxOA5JpxUIxXkrPbR-QQqpIzwbl4nGMlSwalFAfkWUo3nIMGIZ-SA6HKsq5FfUh-7MLUDB5bejouIWJy6B1S61t6YXuP8-DoJ0zB2zV9Ntl-8D0NHb2Kw2Tj90y5GPZfbY_0YuijnYfg6Rzo52HGtIInbplzp-9GO01b-TqtIpdoI8v5aOO98fOfyZfWh72NORwxPSdPOjsmfHH3HpHr03dfdh_Y-cf3Z7uTc-YKrWamdcOd1aKoayUKbHJUKcdL0daicW1todKigaKqddd0XIDulLZVB5WUFkHKI_Jm093H8G3BNJtpyHuNo_UYlmRAC5HhjD8ABakkrxRk9PU_6E1Yos8fWSlRZJeEyhRsVD5qShE7s9_ubICb1XOzeW6y52b13Nzmnld3ykszYfu745fJGRAbkHLJ9xj_Gv1f1Z_5nLiH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1712463225</pqid></control><display><type>article</type><title>Combined Fluorescence and Magnetic Resonance Imaging of Primary Macrophage Migration to Sites of Acute Inflammation Using Near-Infrared Fluorescent Magnetic Nanoparticles</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Kang, Sungmin ; Lee, Ho Won ; Jeon, Young Hyun ; Singh, Thoudam Debraj ; Choi, Yun Ju ; Park, Ji Young ; Kim, Jun Sung ; Lee, Hyunseung ; Hong, Kwan Soo ; Lee, Inkyu ; Jeong, Shin Young ; Lee, Sang-Woo ; Ha, Jeoung-Hee ; Ahn, Byeong-Cheol ; Lee, Jaetae</creator><creatorcontrib>Kang, Sungmin ; Lee, Ho Won ; Jeon, Young Hyun ; Singh, Thoudam Debraj ; Choi, Yun Ju ; Park, Ji Young ; Kim, Jun Sung ; Lee, Hyunseung ; Hong, Kwan Soo ; Lee, Inkyu ; Jeong, Shin Young ; Lee, Sang-Woo ; Ha, Jeoung-Hee ; Ahn, Byeong-Cheol ; Lee, Jaetae</creatorcontrib><description>Purpose
This study aimed to track the migration of primary macrophages labeled with near-infrared (NIR) fluorescent magnetic nanoparticles toward chemically induced acute inflammatory lesions in mice and to visualize the effect of anti-inflammatory drugs on macrophage migration using combined fluorescence and magnetic resonance imaging (FLI/MRI).
Procedures
Primary macrophages were labeled with NIR fluorescent magnetic nanoparticles, and labeled cells were injected into mice intravenously. One day later, inflammation was induced by subcutaneous injection of 1 % carrageenan (CG) solution to footpads of the right hind leg, and phosphate-buffered saline (PBS) as control treatment was subcutaneously injected to footpad of the left hind leg. To evaluate the effect of drug treatment on macrophage migration, a single dose of dexamethasone (DEX) was intraperitoneally administered to the mice immediately after the induction of inflammation and was followed by combined FLI/MRI at predetermined time points.
Results
No difference in cellular viability or phagocytic activity was observed between the labeled and parent macrophages.
In vivo
optical imaging revealed an increase in FLI signals in CG-injected footpads in a time-dependent manner, but not in PBS-treated footpads. DEX treatment inhibited the migration of the labeled macrophages to the CG-injected footpads, with relative decreases in FLI activity. In accordance with FLI, T
2
*-weighted MR images showed hypo-intense signals in the CG-injected footpads but not in the PBS-injected footpads. The DEX-treated mice did not show a dark signal loss zone on MR images in the CG-treated paw.
Conclusions
We successfully tracked the migration of macrophages to inflammatory lesions using both FLI and MRI with NIR fluorescent magnetic nanoparticles and demonstrated the inhibitory effects of DEX on macrophage migration to inflammation sites.</description><identifier>ISSN: 1536-1632</identifier><identifier>EISSN: 1860-2002</identifier><identifier>DOI: 10.1007/s11307-015-0830-z</identifier><identifier>PMID: 25669929</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Cell Movement - immunology ; Cell Survival ; Fluorescent Dyes ; Imaging ; Inflammation - immunology ; Macrophages - chemistry ; Macrophages - immunology ; Macrophages - metabolism ; Magnetic Resonance Imaging - methods ; Magnetite Nanoparticles ; Medicine ; Medicine & Public Health ; Mice ; Mice, Inbred BALB C ; Molecular Imaging - methods ; Radiology ; Research Article ; Spectrometry, Fluorescence - methods</subject><ispartof>Molecular imaging and biology, 2015-10, Vol.17 (5), p.643-651</ispartof><rights>World Molecular Imaging Society 2015</rights><rights>Academy of Molecular Imaging and Society for Molecular Imaging 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-77b0ca72499524eb72485c062d92bcd9a1872b14897fbf0217f57a8f1833ae133</citedby><cites>FETCH-LOGICAL-c475t-77b0ca72499524eb72485c062d92bcd9a1872b14897fbf0217f57a8f1833ae133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11307-015-0830-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11307-015-0830-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25669929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kang, Sungmin</creatorcontrib><creatorcontrib>Lee, Ho Won</creatorcontrib><creatorcontrib>Jeon, Young Hyun</creatorcontrib><creatorcontrib>Singh, Thoudam Debraj</creatorcontrib><creatorcontrib>Choi, Yun Ju</creatorcontrib><creatorcontrib>Park, Ji Young</creatorcontrib><creatorcontrib>Kim, Jun Sung</creatorcontrib><creatorcontrib>Lee, Hyunseung</creatorcontrib><creatorcontrib>Hong, Kwan Soo</creatorcontrib><creatorcontrib>Lee, Inkyu</creatorcontrib><creatorcontrib>Jeong, Shin Young</creatorcontrib><creatorcontrib>Lee, Sang-Woo</creatorcontrib><creatorcontrib>Ha, Jeoung-Hee</creatorcontrib><creatorcontrib>Ahn, Byeong-Cheol</creatorcontrib><creatorcontrib>Lee, Jaetae</creatorcontrib><title>Combined Fluorescence and Magnetic Resonance Imaging of Primary Macrophage Migration to Sites of Acute Inflammation Using Near-Infrared Fluorescent Magnetic Nanoparticles</title><title>Molecular imaging and biology</title><addtitle>Mol Imaging Biol</addtitle><addtitle>Mol Imaging Biol</addtitle><description>Purpose
This study aimed to track the migration of primary macrophages labeled with near-infrared (NIR) fluorescent magnetic nanoparticles toward chemically induced acute inflammatory lesions in mice and to visualize the effect of anti-inflammatory drugs on macrophage migration using combined fluorescence and magnetic resonance imaging (FLI/MRI).
Procedures
Primary macrophages were labeled with NIR fluorescent magnetic nanoparticles, and labeled cells were injected into mice intravenously. One day later, inflammation was induced by subcutaneous injection of 1 % carrageenan (CG) solution to footpads of the right hind leg, and phosphate-buffered saline (PBS) as control treatment was subcutaneously injected to footpad of the left hind leg. To evaluate the effect of drug treatment on macrophage migration, a single dose of dexamethasone (DEX) was intraperitoneally administered to the mice immediately after the induction of inflammation and was followed by combined FLI/MRI at predetermined time points.
Results
No difference in cellular viability or phagocytic activity was observed between the labeled and parent macrophages.
In vivo
optical imaging revealed an increase in FLI signals in CG-injected footpads in a time-dependent manner, but not in PBS-treated footpads. DEX treatment inhibited the migration of the labeled macrophages to the CG-injected footpads, with relative decreases in FLI activity. In accordance with FLI, T
2
*-weighted MR images showed hypo-intense signals in the CG-injected footpads but not in the PBS-injected footpads. The DEX-treated mice did not show a dark signal loss zone on MR images in the CG-treated paw.
Conclusions
We successfully tracked the migration of macrophages to inflammatory lesions using both FLI and MRI with NIR fluorescent magnetic nanoparticles and demonstrated the inhibitory effects of DEX on macrophage migration to inflammation sites.</description><subject>Animals</subject><subject>Cell Movement - immunology</subject><subject>Cell Survival</subject><subject>Fluorescent Dyes</subject><subject>Imaging</subject><subject>Inflammation - immunology</subject><subject>Macrophages - chemistry</subject><subject>Macrophages - immunology</subject><subject>Macrophages - metabolism</subject><subject>Magnetic Resonance Imaging - methods</subject><subject>Magnetite Nanoparticles</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Imaging - methods</subject><subject>Radiology</subject><subject>Research Article</subject><subject>Spectrometry, Fluorescence - methods</subject><issn>1536-1632</issn><issn>1860-2002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc1u1TAQhS0EoqXwAGyQJTZsTD12HCfL6opCpbZUQNeW40xCqsS-2MmCPhJPiaOUnyIhdeXRzDdnxnMIeQn8LXCujxOA5JpxUIxXkrPbR-QQqpIzwbl4nGMlSwalFAfkWUo3nIMGIZ-SA6HKsq5FfUh-7MLUDB5bejouIWJy6B1S61t6YXuP8-DoJ0zB2zV9Ntl-8D0NHb2Kw2Tj90y5GPZfbY_0YuijnYfg6Rzo52HGtIInbplzp-9GO01b-TqtIpdoI8v5aOO98fOfyZfWh72NORwxPSdPOjsmfHH3HpHr03dfdh_Y-cf3Z7uTc-YKrWamdcOd1aKoayUKbHJUKcdL0daicW1todKigaKqddd0XIDulLZVB5WUFkHKI_Jm093H8G3BNJtpyHuNo_UYlmRAC5HhjD8ABakkrxRk9PU_6E1Yos8fWSlRZJeEyhRsVD5qShE7s9_ubICb1XOzeW6y52b13Nzmnld3ykszYfu745fJGRAbkHLJ9xj_Gv1f1Z_5nLiH</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Kang, Sungmin</creator><creator>Lee, Ho Won</creator><creator>Jeon, Young Hyun</creator><creator>Singh, Thoudam Debraj</creator><creator>Choi, Yun Ju</creator><creator>Park, Ji Young</creator><creator>Kim, Jun Sung</creator><creator>Lee, Hyunseung</creator><creator>Hong, Kwan Soo</creator><creator>Lee, Inkyu</creator><creator>Jeong, Shin Young</creator><creator>Lee, Sang-Woo</creator><creator>Ha, Jeoung-Hee</creator><creator>Ahn, Byeong-Cheol</creator><creator>Lee, Jaetae</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20151001</creationdate><title>Combined Fluorescence and Magnetic Resonance Imaging of Primary Macrophage Migration to Sites of Acute Inflammation Using Near-Infrared Fluorescent Magnetic Nanoparticles</title><author>Kang, Sungmin ; Lee, Ho Won ; Jeon, Young Hyun ; Singh, Thoudam Debraj ; Choi, Yun Ju ; Park, Ji Young ; Kim, Jun Sung ; Lee, Hyunseung ; Hong, Kwan Soo ; Lee, Inkyu ; Jeong, Shin Young ; Lee, Sang-Woo ; Ha, Jeoung-Hee ; Ahn, Byeong-Cheol ; Lee, Jaetae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-77b0ca72499524eb72485c062d92bcd9a1872b14897fbf0217f57a8f1833ae133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Cell Movement - immunology</topic><topic>Cell Survival</topic><topic>Fluorescent Dyes</topic><topic>Imaging</topic><topic>Inflammation - immunology</topic><topic>Macrophages - chemistry</topic><topic>Macrophages - immunology</topic><topic>Macrophages - metabolism</topic><topic>Magnetic Resonance Imaging - methods</topic><topic>Magnetite Nanoparticles</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Imaging - methods</topic><topic>Radiology</topic><topic>Research Article</topic><topic>Spectrometry, Fluorescence - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kang, Sungmin</creatorcontrib><creatorcontrib>Lee, Ho Won</creatorcontrib><creatorcontrib>Jeon, Young Hyun</creatorcontrib><creatorcontrib>Singh, Thoudam Debraj</creatorcontrib><creatorcontrib>Choi, Yun Ju</creatorcontrib><creatorcontrib>Park, Ji Young</creatorcontrib><creatorcontrib>Kim, Jun Sung</creatorcontrib><creatorcontrib>Lee, Hyunseung</creatorcontrib><creatorcontrib>Hong, Kwan Soo</creatorcontrib><creatorcontrib>Lee, Inkyu</creatorcontrib><creatorcontrib>Jeong, Shin Young</creatorcontrib><creatorcontrib>Lee, Sang-Woo</creatorcontrib><creatorcontrib>Ha, Jeoung-Hee</creatorcontrib><creatorcontrib>Ahn, Byeong-Cheol</creatorcontrib><creatorcontrib>Lee, Jaetae</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Molecular imaging and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kang, Sungmin</au><au>Lee, Ho Won</au><au>Jeon, Young Hyun</au><au>Singh, Thoudam Debraj</au><au>Choi, Yun Ju</au><au>Park, Ji Young</au><au>Kim, Jun Sung</au><au>Lee, Hyunseung</au><au>Hong, Kwan Soo</au><au>Lee, Inkyu</au><au>Jeong, Shin Young</au><au>Lee, Sang-Woo</au><au>Ha, Jeoung-Hee</au><au>Ahn, Byeong-Cheol</au><au>Lee, Jaetae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined Fluorescence and Magnetic Resonance Imaging of Primary Macrophage Migration to Sites of Acute Inflammation Using Near-Infrared Fluorescent Magnetic Nanoparticles</atitle><jtitle>Molecular imaging and biology</jtitle><stitle>Mol Imaging Biol</stitle><addtitle>Mol Imaging Biol</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>17</volume><issue>5</issue><spage>643</spage><epage>651</epage><pages>643-651</pages><issn>1536-1632</issn><eissn>1860-2002</eissn><abstract>Purpose
This study aimed to track the migration of primary macrophages labeled with near-infrared (NIR) fluorescent magnetic nanoparticles toward chemically induced acute inflammatory lesions in mice and to visualize the effect of anti-inflammatory drugs on macrophage migration using combined fluorescence and magnetic resonance imaging (FLI/MRI).
Procedures
Primary macrophages were labeled with NIR fluorescent magnetic nanoparticles, and labeled cells were injected into mice intravenously. One day later, inflammation was induced by subcutaneous injection of 1 % carrageenan (CG) solution to footpads of the right hind leg, and phosphate-buffered saline (PBS) as control treatment was subcutaneously injected to footpad of the left hind leg. To evaluate the effect of drug treatment on macrophage migration, a single dose of dexamethasone (DEX) was intraperitoneally administered to the mice immediately after the induction of inflammation and was followed by combined FLI/MRI at predetermined time points.
Results
No difference in cellular viability or phagocytic activity was observed between the labeled and parent macrophages.
In vivo
optical imaging revealed an increase in FLI signals in CG-injected footpads in a time-dependent manner, but not in PBS-treated footpads. DEX treatment inhibited the migration of the labeled macrophages to the CG-injected footpads, with relative decreases in FLI activity. In accordance with FLI, T
2
*-weighted MR images showed hypo-intense signals in the CG-injected footpads but not in the PBS-injected footpads. The DEX-treated mice did not show a dark signal loss zone on MR images in the CG-treated paw.
Conclusions
We successfully tracked the migration of macrophages to inflammatory lesions using both FLI and MRI with NIR fluorescent magnetic nanoparticles and demonstrated the inhibitory effects of DEX on macrophage migration to inflammation sites.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25669929</pmid><doi>10.1007/s11307-015-0830-z</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular imaging and biology, 2015-10, Vol.17 (5), p.643-651 |
| issn | 1536-1632 1860-2002 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Cell Movement - immunology Cell Survival Fluorescent Dyes Imaging Inflammation - immunology Macrophages - chemistry Macrophages - immunology Macrophages - metabolism Magnetic Resonance Imaging - methods Magnetite Nanoparticles Medicine Medicine & Public Health Mice Mice, Inbred BALB C Molecular Imaging - methods Radiology Research Article Spectrometry, Fluorescence - methods |
| title | Combined Fluorescence and Magnetic Resonance Imaging of Primary Macrophage Migration to Sites of Acute Inflammation Using Near-Infrared Fluorescent Magnetic Nanoparticles |
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