Microbial source markers assessment in the Bogotá River basin (Colombia)
The microbiological indicators traditionally used to assess fecal contamination are insufficient to identify the source. The aim of this study was to detect microbial markers to identify the source of fecal pollution in the Bogotá River (Colombia). For this, we determined non-discriminating indicato...
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| Veröffentlicht in: | Journal of water and health 2015-09, Vol.13 (3), p.801-810 |
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| creator | Venegas, Camilo Diez, Hugo Blanch, Anicet R Jofre, Juan Campos, Claudia |
| description | The microbiological indicators traditionally used to assess fecal contamination are insufficient to identify the source. The aim of this study was to detect microbial markers to identify the source of fecal pollution in the Bogotá River (Colombia). For this, we determined non-discriminating indicators such as Escherichia coli, somatic coliphages and phages infecting strain RYC2056 of Bacteroides, and potential source tracking markers as phages infecting strains GA17, HB13, and CA8 of Bacteroides, sorbitol-fermenting bifidobacteria, and molecular markers of Bifidobacterium adolescentis, Bifiodobacterium dentium, and Bacteroidetes in raw municipal wastewaters, slaughterhouse wastewaters, and the Bogotá River. Bacteriophages infecting Bacteroides strain GA17 and the molecular markers identified the wastewater sources. In contrast, sorbitol-fermenting bifidobacteria failed regarding specificity. In the Bogotá River, phages infecting strain GA17 were detected in all samples downstream of Bogotá, whereas they should be concentrated from 1 l samples in upstream samples containing less than 10(3) E. coli/100 ml to be detected. In the river water, the fraction of positive detections of molecular markers was lower than that of phages infecting strain GA17. The ratio SOMCPH/GA17PH was shown also to be a good marker. These results provide information that will allow focusing measures for sanitation of the Bogotá River. |
| doi_str_mv | 10.2166/wh.2015.240 |
| format | Article |
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The aim of this study was to detect microbial markers to identify the source of fecal pollution in the Bogotá River (Colombia). For this, we determined non-discriminating indicators such as Escherichia coli, somatic coliphages and phages infecting strain RYC2056 of Bacteroides, and potential source tracking markers as phages infecting strains GA17, HB13, and CA8 of Bacteroides, sorbitol-fermenting bifidobacteria, and molecular markers of Bifidobacterium adolescentis, Bifiodobacterium dentium, and Bacteroidetes in raw municipal wastewaters, slaughterhouse wastewaters, and the Bogotá River. Bacteriophages infecting Bacteroides strain GA17 and the molecular markers identified the wastewater sources. In contrast, sorbitol-fermenting bifidobacteria failed regarding specificity. In the Bogotá River, phages infecting strain GA17 were detected in all samples downstream of Bogotá, whereas they should be concentrated from 1 l samples in upstream samples containing less than 10(3) E. coli/100 ml to be detected. In the river water, the fraction of positive detections of molecular markers was lower than that of phages infecting strain GA17. The ratio SOMCPH/GA17PH was shown also to be a good marker. These results provide information that will allow focusing measures for sanitation of the Bogotá River.</description><identifier>ISSN: 1477-8920</identifier><identifier>EISSN: 1996-7829</identifier><identifier>DOI: 10.2166/wh.2015.240</identifier><identifier>PMID: 26322765</identifier><language>eng</language><publisher>England: IWA Publishing</publisher><subject>Abattoirs ; Bacteriophages ; Bacteroides ; Bacteroides - genetics ; Bacteroides fragilis ; Bacteroidetes ; Bifidobacterium adolescentis ; Colombia ; Contamination ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; E coli ; Fecal coliforms ; Feces ; Fermentation ; Freshwater ; Genes, Bacterial - genetics ; Genetic Markers - genetics ; Indicators ; Markers ; Microorganisms ; Mitochondrial DNA ; Municipal wastewater ; Phages ; Pollution ; Ribosomal DNA ; River basins ; River water ; Rivers ; Rivers - microbiology ; Sanitation ; Sorbitol ; Specificity ; Waste Water - microbiology ; Wastewater ; Water pollution</subject><ispartof>Journal of water and health, 2015-09, Vol.13 (3), p.801-810</ispartof><rights>Copyright IWA Publishing Sep 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-468ed95dfa6c7947c22f59fb591984a7962fe35a33fc03cc5f61b03bffb869f33</citedby><cites>FETCH-LOGICAL-c387t-468ed95dfa6c7947c22f59fb591984a7962fe35a33fc03cc5f61b03bffb869f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26322765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Venegas, Camilo</creatorcontrib><creatorcontrib>Diez, Hugo</creatorcontrib><creatorcontrib>Blanch, Anicet R</creatorcontrib><creatorcontrib>Jofre, Juan</creatorcontrib><creatorcontrib>Campos, Claudia</creatorcontrib><title>Microbial source markers assessment in the Bogotá River basin (Colombia)</title><title>Journal of water and health</title><addtitle>J Water Health</addtitle><description>The microbiological indicators traditionally used to assess fecal contamination are insufficient to identify the source. The aim of this study was to detect microbial markers to identify the source of fecal pollution in the Bogotá River (Colombia). For this, we determined non-discriminating indicators such as Escherichia coli, somatic coliphages and phages infecting strain RYC2056 of Bacteroides, and potential source tracking markers as phages infecting strains GA17, HB13, and CA8 of Bacteroides, sorbitol-fermenting bifidobacteria, and molecular markers of Bifidobacterium adolescentis, Bifiodobacterium dentium, and Bacteroidetes in raw municipal wastewaters, slaughterhouse wastewaters, and the Bogotá River. Bacteriophages infecting Bacteroides strain GA17 and the molecular markers identified the wastewater sources. In contrast, sorbitol-fermenting bifidobacteria failed regarding specificity. In the Bogotá River, phages infecting strain GA17 were detected in all samples downstream of Bogotá, whereas they should be concentrated from 1 l samples in upstream samples containing less than 10(3) E. coli/100 ml to be detected. In the river water, the fraction of positive detections of molecular markers was lower than that of phages infecting strain GA17. The ratio SOMCPH/GA17PH was shown also to be a good marker. These results provide information that will allow focusing measures for sanitation of the Bogotá River.</description><subject>Abattoirs</subject><subject>Bacteriophages</subject><subject>Bacteroides</subject><subject>Bacteroides - genetics</subject><subject>Bacteroides fragilis</subject><subject>Bacteroidetes</subject><subject>Bifidobacterium adolescentis</subject><subject>Colombia</subject><subject>Contamination</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>E coli</subject><subject>Fecal coliforms</subject><subject>Feces</subject><subject>Fermentation</subject><subject>Freshwater</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetic Markers - genetics</subject><subject>Indicators</subject><subject>Markers</subject><subject>Microorganisms</subject><subject>Mitochondrial DNA</subject><subject>Municipal wastewater</subject><subject>Phages</subject><subject>Pollution</subject><subject>Ribosomal DNA</subject><subject>River basins</subject><subject>River water</subject><subject>Rivers</subject><subject>Rivers - microbiology</subject><subject>Sanitation</subject><subject>Sorbitol</subject><subject>Specificity</subject><subject>Waste Water - microbiology</subject><subject>Wastewater</subject><subject>Water pollution</subject><issn>1477-8920</issn><issn>1996-7829</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1LAzEQhoMotlZP3mXBS0W2JpOvzVGLH4WKIHoO2TSxW3e7dbO1-HP8Lf4xU1o9ePI0w_DMwDwvQscED4AIcbGaDgATPgCGd1CXKCVSmYHajT2TMs0U4A46CGGGMQjgsI86ICiAFLyLRveFbeq8MGUS6mVjXVKZ5tU1ITEhuBAqN2-TYp60U5dc1S91-_WZPBbvrklyE-K8P6zLuor7Z4doz5syuKNt7aHnm-un4V06frgdDS_HqaWZbFMmMjdRfOKNsFIxaQE8Vz7niqiMGakEeEe5odRbTK3lXpAc09z7PBPKU9pD_c3dRVO_LV1odVUE68rSzF29DJpIACIlMPEPFGcKM8xZRE__oLOoYx4f0URRrARhREbqfENFZyE0zutFU0RhH5pgvQ5Dr6Z6HYaOYUT6ZHtzmVdu8sv-2KffPLeDXQ</recordid><startdate>201509</startdate><enddate>201509</enddate><creator>Venegas, Camilo</creator><creator>Diez, Hugo</creator><creator>Blanch, Anicet R</creator><creator>Jofre, Juan</creator><creator>Campos, Claudia</creator><general>IWA Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>7X8</scope><scope>7ST</scope><scope>7TV</scope><scope>7U9</scope><scope>H94</scope><scope>SOI</scope></search><sort><creationdate>201509</creationdate><title>Microbial source markers assessment in the Bogotá River basin (Colombia)</title><author>Venegas, Camilo ; Diez, Hugo ; Blanch, Anicet R ; Jofre, Juan ; Campos, Claudia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-468ed95dfa6c7947c22f59fb591984a7962fe35a33fc03cc5f61b03bffb869f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abattoirs</topic><topic>Bacteriophages</topic><topic>Bacteroides</topic><topic>Bacteroides - genetics</topic><topic>Bacteroides fragilis</topic><topic>Bacteroidetes</topic><topic>Bifidobacterium adolescentis</topic><topic>Colombia</topic><topic>Contamination</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>E coli</topic><topic>Fecal coliforms</topic><topic>Feces</topic><topic>Fermentation</topic><topic>Freshwater</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetic Markers - genetics</topic><topic>Indicators</topic><topic>Markers</topic><topic>Microorganisms</topic><topic>Mitochondrial DNA</topic><topic>Municipal wastewater</topic><topic>Phages</topic><topic>Pollution</topic><topic>Ribosomal DNA</topic><topic>River basins</topic><topic>River water</topic><topic>Rivers</topic><topic>Rivers - microbiology</topic><topic>Sanitation</topic><topic>Sorbitol</topic><topic>Specificity</topic><topic>Waste Water - microbiology</topic><topic>Wastewater</topic><topic>Water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Venegas, Camilo</creatorcontrib><creatorcontrib>Diez, Hugo</creatorcontrib><creatorcontrib>Blanch, Anicet R</creatorcontrib><creatorcontrib>Jofre, Juan</creatorcontrib><creatorcontrib>Campos, Claudia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of water and health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Venegas, Camilo</au><au>Diez, Hugo</au><au>Blanch, Anicet R</au><au>Jofre, Juan</au><au>Campos, Claudia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial source markers assessment in the Bogotá River basin (Colombia)</atitle><jtitle>Journal of water and health</jtitle><addtitle>J Water Health</addtitle><date>2015-09</date><risdate>2015</risdate><volume>13</volume><issue>3</issue><spage>801</spage><epage>810</epage><pages>801-810</pages><issn>1477-8920</issn><eissn>1996-7829</eissn><abstract>The microbiological indicators traditionally used to assess fecal contamination are insufficient to identify the source. The aim of this study was to detect microbial markers to identify the source of fecal pollution in the Bogotá River (Colombia). For this, we determined non-discriminating indicators such as Escherichia coli, somatic coliphages and phages infecting strain RYC2056 of Bacteroides, and potential source tracking markers as phages infecting strains GA17, HB13, and CA8 of Bacteroides, sorbitol-fermenting bifidobacteria, and molecular markers of Bifidobacterium adolescentis, Bifiodobacterium dentium, and Bacteroidetes in raw municipal wastewaters, slaughterhouse wastewaters, and the Bogotá River. Bacteriophages infecting Bacteroides strain GA17 and the molecular markers identified the wastewater sources. In contrast, sorbitol-fermenting bifidobacteria failed regarding specificity. In the Bogotá River, phages infecting strain GA17 were detected in all samples downstream of Bogotá, whereas they should be concentrated from 1 l samples in upstream samples containing less than 10(3) E. coli/100 ml to be detected. In the river water, the fraction of positive detections of molecular markers was lower than that of phages infecting strain GA17. The ratio SOMCPH/GA17PH was shown also to be a good marker. These results provide information that will allow focusing measures for sanitation of the Bogotá River.</abstract><cop>England</cop><pub>IWA Publishing</pub><pmid>26322765</pmid><doi>10.2166/wh.2015.240</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1477-8920 |
| ispartof | Journal of water and health, 2015-09, Vol.13 (3), p.801-810 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Abattoirs Bacteriophages Bacteroides Bacteroides - genetics Bacteroides fragilis Bacteroidetes Bifidobacterium adolescentis Colombia Contamination Deoxyribonucleic acid DNA DNA, Bacterial - analysis DNA, Bacterial - genetics E coli Fecal coliforms Feces Fermentation Freshwater Genes, Bacterial - genetics Genetic Markers - genetics Indicators Markers Microorganisms Mitochondrial DNA Municipal wastewater Phages Pollution Ribosomal DNA River basins River water Rivers Rivers - microbiology Sanitation Sorbitol Specificity Waste Water - microbiology Wastewater Water pollution |
| title | Microbial source markers assessment in the Bogotá River basin (Colombia) |
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