Detection and molecular characterization of zoonotic viruses in swine fecal samples in Italian pig herds
Gastrointestinal disease is frequent in pigs, and among the different etiological agents involved, viruses are considered the leading cause of infection in this animal species. Furthermore, about half of the newly identified swine pathogens are viruses, many of which may be transmitted to humans by...
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| Veröffentlicht in: | Archives of virology 2015-10, Vol.160 (10), p.2547-2556 |
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| description | Gastrointestinal disease is frequent in pigs, and among the different etiological agents involved, viruses are considered the leading cause of infection in this animal species. Furthermore, about half of the newly identified swine pathogens are viruses, many of which may be transmitted to humans by direct contact or by indirect transmission pathways. In this study, the prevalence of astrovirus (AstV), group A rotavirus (RVA), norovirus (NoV) and hepatitis E virus (HEV) infections in pigs was investigated. During 2012-2014, 242 fecal samples were collected from pigs at different production stages (5 to 220 days old) on eight swine farms located in northern, central and southern Italy. Seven out of eight farms analyzed were positive for AstV, which was detected in 163 out of 242 (67.4 %) samples and was the most prevalent virus; 61 of the 163 AstV-positive animals (37.4 %) had diarrhea. HEV was detected on six farms and in 45 (18.6 %) of the 242 samples analyzed. Twenty-three HEV-infected pigs had diarrhea (51.1 %). A lower prevalence was observed for RVA, which was found in 10 of the 242 samples (4.1 %) from three positive farms, and diarrhea was present only in six infected pigs (60.0 %). No swine samples were found to be positive for NoV. Genetic diversity and phylogenetic relationships of some strains representative of the different viruses detected were investigated, confirming a wide heterogeneity of viral strains circulating among pigs. |
| doi_str_mv | 10.1007/s00705-015-2538-4 |
| format | Article |
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Furthermore, about half of the newly identified swine pathogens are viruses, many of which may be transmitted to humans by direct contact or by indirect transmission pathways. In this study, the prevalence of astrovirus (AstV), group A rotavirus (RVA), norovirus (NoV) and hepatitis E virus (HEV) infections in pigs was investigated. During 2012-2014, 242 fecal samples were collected from pigs at different production stages (5 to 220 days old) on eight swine farms located in northern, central and southern Italy. Seven out of eight farms analyzed were positive for AstV, which was detected in 163 out of 242 (67.4 %) samples and was the most prevalent virus; 61 of the 163 AstV-positive animals (37.4 %) had diarrhea. HEV was detected on six farms and in 45 (18.6 %) of the 242 samples analyzed. Twenty-three HEV-infected pigs had diarrhea (51.1 %). A lower prevalence was observed for RVA, which was found in 10 of the 242 samples (4.1 %) from three positive farms, and diarrhea was present only in six infected pigs (60.0 %). No swine samples were found to be positive for NoV. Genetic diversity and phylogenetic relationships of some strains representative of the different viruses detected were investigated, confirming a wide heterogeneity of viral strains circulating among pigs.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-015-2538-4</identifier><identifier>PMID: 26215443</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Animals ; Astrovirus ; Asymptomatic ; Biomedical and Life Sciences ; Biomedicine ; Developing countries ; Diarrhea ; direct contact ; farms ; Feces ; Feces - virology ; Food contamination & poisoning ; Gastroenteritis ; Genetic Variation ; Genomes ; Genotype ; Genotype & phenotype ; Group a rotavirus ; Hepatitis ; Hepatitis E virus ; herds ; Hogs ; humans ; Infectious Diseases ; Italy ; LDCs ; Medical Microbiology ; Molecular Sequence Data ; Norovirus ; Original Article ; Pathogens ; Phylogeny ; Proteins ; Rotavirus ; Rotavirus A ; Swine ; Swine Diseases - virology ; Virology ; Virus Diseases - veterinary ; Virus Diseases - virology ; Viruses ; Viruses - classification ; Viruses - genetics ; Viruses - isolation & purification ; Zoonoses ; Zoonoses - virology</subject><ispartof>Archives of virology, 2015-10, Vol.160 (10), p.2547-2556</ispartof><rights>Springer-Verlag Wien 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-56ec2f925cde9d2a3bc5b171d463357539254d99fa372438bb1efaef197823233</citedby><cites>FETCH-LOGICAL-c495t-56ec2f925cde9d2a3bc5b171d463357539254d99fa372438bb1efaef197823233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00705-015-2538-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00705-015-2538-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26215443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Monini, Marina</creatorcontrib><creatorcontrib>Di Bartolo, Ilaria</creatorcontrib><creatorcontrib>Ianiro, Giovanni</creatorcontrib><creatorcontrib>Angeloni, Giorgia</creatorcontrib><creatorcontrib>Magistrali, Chiara Francesca</creatorcontrib><creatorcontrib>Ostanello, Fabio</creatorcontrib><creatorcontrib>Ruggeri, Franco Maria</creatorcontrib><title>Detection and molecular characterization of zoonotic viruses in swine fecal samples in Italian pig herds</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><addtitle>Arch Virol</addtitle><description>Gastrointestinal disease is frequent in pigs, and among the different etiological agents involved, viruses are considered the leading cause of infection in this animal species. Furthermore, about half of the newly identified swine pathogens are viruses, many of which may be transmitted to humans by direct contact or by indirect transmission pathways. In this study, the prevalence of astrovirus (AstV), group A rotavirus (RVA), norovirus (NoV) and hepatitis E virus (HEV) infections in pigs was investigated. During 2012-2014, 242 fecal samples were collected from pigs at different production stages (5 to 220 days old) on eight swine farms located in northern, central and southern Italy. Seven out of eight farms analyzed were positive for AstV, which was detected in 163 out of 242 (67.4 %) samples and was the most prevalent virus; 61 of the 163 AstV-positive animals (37.4 %) had diarrhea. HEV was detected on six farms and in 45 (18.6 %) of the 242 samples analyzed. Twenty-three HEV-infected pigs had diarrhea (51.1 %). A lower prevalence was observed for RVA, which was found in 10 of the 242 samples (4.1 %) from three positive farms, and diarrhea was present only in six infected pigs (60.0 %). No swine samples were found to be positive for NoV. Genetic diversity and phylogenetic relationships of some strains representative of the different viruses detected were investigated, confirming a wide heterogeneity of viral strains circulating among pigs.</description><subject>Animals</subject><subject>Astrovirus</subject><subject>Asymptomatic</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Developing countries</subject><subject>Diarrhea</subject><subject>direct contact</subject><subject>farms</subject><subject>Feces</subject><subject>Feces - virology</subject><subject>Food contamination & poisoning</subject><subject>Gastroenteritis</subject><subject>Genetic Variation</subject><subject>Genomes</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Group a rotavirus</subject><subject>Hepatitis</subject><subject>Hepatitis E virus</subject><subject>herds</subject><subject>Hogs</subject><subject>humans</subject><subject>Infectious Diseases</subject><subject>Italy</subject><subject>LDCs</subject><subject>Medical Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Norovirus</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Rotavirus</subject><subject>Rotavirus A</subject><subject>Swine</subject><subject>Swine Diseases - virology</subject><subject>Virology</subject><subject>Virus Diseases - veterinary</subject><subject>Virus Diseases - virology</subject><subject>Viruses</subject><subject>Viruses - classification</subject><subject>Viruses - genetics</subject><subject>Viruses - isolation & purification</subject><subject>Zoonoses</subject><subject>Zoonoses - virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkU9rFTEUxYNY7LP6AdxooBs3U_N3MllKrVoouNCuQyZz817KTPJMZhT76c3rVJEuipsE7vmdc7kchF5RckYJUe9KfYhsCJUNk7xrxBO0oYKzplO6e4o2hBPRdC3pjtHzUm4IqQMun6Fj1jIqheAbtPsAM7g5pIhtHPCURnDLaDN2O5utmyGHW3snJ49vU4ppDg7_CHkpUHCIuPwMEbAHZ0dc7LQf1_HlbMdgI96HLd5BHsoLdOTtWODl_X-Crj9efDv_3Fx9-XR5_v6qcULLuZEtOOY1k24APTDLeyd7quggWs6lkrxKYtDaW66Y4F3fU_AWPNWqY5xxfoLerrn7nL4vUGYzheJgHG2EtBRDFWNUiRrzHyhttaC6JRU9fYDepCXHesgd1SrN6GE3XSmXUykZvNnnMNn8y1BiDo2ZtTFTGzOHxoyontf3yUs_wfDX8aeiCrAVKFWKW8j_rH4k9c1q8jYZu82hmOuvjNCWEMqUEB3_DR5gqUg</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Monini, Marina</creator><creator>Di Bartolo, Ilaria</creator><creator>Ianiro, Giovanni</creator><creator>Angeloni, Giorgia</creator><creator>Magistrali, Chiara Francesca</creator><creator>Ostanello, Fabio</creator><creator>Ruggeri, Franco Maria</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20151001</creationdate><title>Detection and molecular characterization of zoonotic viruses in swine fecal samples in Italian pig herds</title><author>Monini, Marina ; Di Bartolo, Ilaria ; Ianiro, Giovanni ; Angeloni, Giorgia ; Magistrali, Chiara Francesca ; Ostanello, Fabio ; Ruggeri, Franco Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-56ec2f925cde9d2a3bc5b171d463357539254d99fa372438bb1efaef197823233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Astrovirus</topic><topic>Asymptomatic</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Developing countries</topic><topic>Diarrhea</topic><topic>direct contact</topic><topic>farms</topic><topic>Feces</topic><topic>Feces - 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Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Monini, Marina</au><au>Di Bartolo, Ilaria</au><au>Ianiro, Giovanni</au><au>Angeloni, Giorgia</au><au>Magistrali, Chiara Francesca</au><au>Ostanello, Fabio</au><au>Ruggeri, Franco Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and molecular characterization of zoonotic viruses in swine fecal samples in Italian pig herds</atitle><jtitle>Archives of virology</jtitle><stitle>Arch Virol</stitle><addtitle>Arch Virol</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>160</volume><issue>10</issue><spage>2547</spage><epage>2556</epage><pages>2547-2556</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Gastrointestinal disease is frequent in pigs, and among the different etiological agents involved, viruses are considered the leading cause of infection in this animal species. Furthermore, about half of the newly identified swine pathogens are viruses, many of which may be transmitted to humans by direct contact or by indirect transmission pathways. In this study, the prevalence of astrovirus (AstV), group A rotavirus (RVA), norovirus (NoV) and hepatitis E virus (HEV) infections in pigs was investigated. During 2012-2014, 242 fecal samples were collected from pigs at different production stages (5 to 220 days old) on eight swine farms located in northern, central and southern Italy. Seven out of eight farms analyzed were positive for AstV, which was detected in 163 out of 242 (67.4 %) samples and was the most prevalent virus; 61 of the 163 AstV-positive animals (37.4 %) had diarrhea. HEV was detected on six farms and in 45 (18.6 %) of the 242 samples analyzed. Twenty-three HEV-infected pigs had diarrhea (51.1 %). A lower prevalence was observed for RVA, which was found in 10 of the 242 samples (4.1 %) from three positive farms, and diarrhea was present only in six infected pigs (60.0 %). No swine samples were found to be positive for NoV. Genetic diversity and phylogenetic relationships of some strains representative of the different viruses detected were investigated, confirming a wide heterogeneity of viral strains circulating among pigs.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>26215443</pmid><doi>10.1007/s00705-015-2538-4</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Astrovirus Asymptomatic Biomedical and Life Sciences Biomedicine Developing countries Diarrhea direct contact farms Feces Feces - virology Food contamination & poisoning Gastroenteritis Genetic Variation Genomes Genotype Genotype & phenotype Group a rotavirus Hepatitis Hepatitis E virus herds Hogs humans Infectious Diseases Italy LDCs Medical Microbiology Molecular Sequence Data Norovirus Original Article Pathogens Phylogeny Proteins Rotavirus Rotavirus A Swine Swine Diseases - virology Virology Virus Diseases - veterinary Virus Diseases - virology Viruses Viruses - classification Viruses - genetics Viruses - isolation & purification Zoonoses Zoonoses - virology |
| title | Detection and molecular characterization of zoonotic viruses in swine fecal samples in Italian pig herds |
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