Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds
Human endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However,...
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| Veröffentlicht in: | Molecular neurobiology 2015-12, Vol.52 (3), p.1704-1713 |
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| creator | Ebrahimi-Barough, Somayeh Norouzi Javidan, Abbas Saberi, Hoshangh Joghataei, Mohammad Tghi Rahbarghazi, Reza Mirzaei, Esmaeil Faghihi, Faezeh Shirian, Sadegh Ai, Armin Ai, Jafar |
| description | Human endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15 days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases. |
| doi_str_mv | 10.1007/s12035-014-8931-2 |
| format | Article |
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The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15 days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases.</description><identifier>ISSN: 0893-7648</identifier><identifier>EISSN: 1559-1182</identifier><identifier>DOI: 10.1007/s12035-014-8931-2</identifier><identifier>PMID: 25377792</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Absorbable Implants ; Biochemistry ; Biodegradation ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell Differentiation - drug effects ; Cell Lineage - drug effects ; Cell Lineage - physiology ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Humans ; Lactic Acid - pharmacology ; Motor ability ; Motor Neurons - cytology ; Nanofibers ; Nervous system ; Neurobiology ; Neurology ; Neurons ; Neurosciences ; Polyglycolic Acid - pharmacology ; Regeneration - drug effects ; Stem cells ; Stem Cells - cytology ; Tissues</subject><ispartof>Molecular neurobiology, 2015-12, Vol.52 (3), p.1704-1713</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-de0589c969d3bbb6dfa245fb76adf7180c3a5e683d45eeba61291fdd126a75e93</citedby><cites>FETCH-LOGICAL-c475t-de0589c969d3bbb6dfa245fb76adf7180c3a5e683d45eeba61291fdd126a75e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12035-014-8931-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12035-014-8931-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25377792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ebrahimi-Barough, Somayeh</creatorcontrib><creatorcontrib>Norouzi Javidan, Abbas</creatorcontrib><creatorcontrib>Saberi, Hoshangh</creatorcontrib><creatorcontrib>Joghataei, Mohammad Tghi</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Mirzaei, Esmaeil</creatorcontrib><creatorcontrib>Faghihi, Faezeh</creatorcontrib><creatorcontrib>Shirian, Sadegh</creatorcontrib><creatorcontrib>Ai, Armin</creatorcontrib><creatorcontrib>Ai, Jafar</creatorcontrib><title>Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds</title><title>Molecular neurobiology</title><addtitle>Mol Neurobiol</addtitle><addtitle>Mol Neurobiol</addtitle><description>Human endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15 days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases.</description><subject>Absorbable Implants</subject><subject>Biochemistry</subject><subject>Biodegradation</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Lineage - drug effects</subject><subject>Cell Lineage - physiology</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Humans</subject><subject>Lactic Acid - pharmacology</subject><subject>Motor ability</subject><subject>Motor Neurons - cytology</subject><subject>Nanofibers</subject><subject>Nervous system</subject><subject>Neurobiology</subject><subject>Neurology</subject><subject>Neurons</subject><subject>Neurosciences</subject><subject>Polyglycolic Acid - pharmacology</subject><subject>Regeneration - drug effects</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Tissues</subject><issn>0893-7648</issn><issn>1559-1182</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkU1v1DAQhi0EarcfP4ALssSFi4vHie34WJbth7QUpJaz5cTjNiUbt3aC1H-PV1sqhITEybLnmXc0fgh5C_wEONcfMwheScahZo2pgIlXZAFSGgbQiNdkwcsr06pu9slBzvecCwFc75F9ISuttRELcrf66YbZTX0caQz0S5xiolc4pziydf8D6RKHgX7uQ8CE49S_kHer8XqZabl86qPH2-S8awek39bnp_TKjTH0LSZ63bkQ4uDzEXkT3JDx-Pk8JN_PVjfLC7b-en65PF2zrtZyYh65bExnlPFV27bKBydqGVqtnA8aGt5VTqJqKl9LxNYpEAaC9yCU0xJNdUg-7HIfUnycMU920-euLOFGjHO2oMsnaDCN_A-0zNNKmW3q-7_Q-zinsSyypUqkFEIVCnZUl2LOCYN9SP3GpScL3G6N2Z0xW4zZrTErSs-75-S53aB_6fitqABiB-RSGm8x_TH6n6m_AMZUn8g</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Ebrahimi-Barough, Somayeh</creator><creator>Norouzi Javidan, Abbas</creator><creator>Saberi, Hoshangh</creator><creator>Joghataei, Mohammad Tghi</creator><creator>Rahbarghazi, Reza</creator><creator>Mirzaei, Esmaeil</creator><creator>Faghihi, Faezeh</creator><creator>Shirian, Sadegh</creator><creator>Ai, Armin</creator><creator>Ai, Jafar</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QR</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>7T7</scope><scope>C1K</scope></search><sort><creationdate>20151201</creationdate><title>Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds</title><author>Ebrahimi-Barough, Somayeh ; Norouzi Javidan, Abbas ; Saberi, Hoshangh ; Joghataei, Mohammad Tghi ; Rahbarghazi, Reza ; Mirzaei, Esmaeil ; Faghihi, Faezeh ; Shirian, Sadegh ; Ai, Armin ; Ai, Jafar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-de0589c969d3bbb6dfa245fb76adf7180c3a5e683d45eeba61291fdd126a75e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Absorbable Implants</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Lineage - drug effects</topic><topic>Cell Lineage - physiology</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Humans</topic><topic>Lactic Acid - pharmacology</topic><topic>Motor ability</topic><topic>Motor Neurons - cytology</topic><topic>Nanofibers</topic><topic>Nervous system</topic><topic>Neurobiology</topic><topic>Neurology</topic><topic>Neurons</topic><topic>Neurosciences</topic><topic>Polyglycolic Acid - pharmacology</topic><topic>Regeneration - drug effects</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebrahimi-Barough, Somayeh</creatorcontrib><creatorcontrib>Norouzi Javidan, Abbas</creatorcontrib><creatorcontrib>Saberi, Hoshangh</creatorcontrib><creatorcontrib>Joghataei, Mohammad Tghi</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Mirzaei, Esmaeil</creatorcontrib><creatorcontrib>Faghihi, Faezeh</creatorcontrib><creatorcontrib>Shirian, Sadegh</creatorcontrib><creatorcontrib>Ai, Armin</creatorcontrib><creatorcontrib>Ai, Jafar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ebrahimi-Barough, Somayeh</au><au>Norouzi Javidan, Abbas</au><au>Saberi, Hoshangh</au><au>Joghataei, Mohammad Tghi</au><au>Rahbarghazi, Reza</au><au>Mirzaei, Esmaeil</au><au>Faghihi, Faezeh</au><au>Shirian, Sadegh</au><au>Ai, Armin</au><au>Ai, Jafar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds</atitle><jtitle>Molecular neurobiology</jtitle><stitle>Mol Neurobiol</stitle><addtitle>Mol Neurobiol</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>52</volume><issue>3</issue><spage>1704</spage><epage>1713</epage><pages>1704-1713</pages><issn>0893-7648</issn><eissn>1559-1182</eissn><abstract>Human endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15 days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25377792</pmid><doi>10.1007/s12035-014-8931-2</doi><tpages>10</tpages></addata></record> |
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| subjects | Absorbable Implants Biochemistry Biodegradation Biomedical and Life Sciences Biomedicine Cell Biology Cell Differentiation - drug effects Cell Lineage - drug effects Cell Lineage - physiology Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Humans Lactic Acid - pharmacology Motor ability Motor Neurons - cytology Nanofibers Nervous system Neurobiology Neurology Neurons Neurosciences Polyglycolic Acid - pharmacology Regeneration - drug effects Stem cells Stem Cells - cytology Tissues |
| title | Evaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds |
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