Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody

We report the adaptation of the new CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR‐associated protein 9) system to disrupt the gene encoding fucosyltransferase 8 (FUT8), an α1,6‐fucosyltransferase that directs fucose addition to derived antibody Fc region asparagine 297...

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Veröffentlicht in:	Engineering in life sciences 2015-09, Vol.15 (6), p.660-666
Hauptverfasser:	Sun, Tao, Li, Chaodong, Han, Lei, Jiang, Hua, Xie, Yueqing, Zhang, Baohong, Qian, Xiuping, Lu, Huili, Zhu, Jianwei
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibody Antibody-dependent cellular cytotoxicity CHO-K1 cells CRISPR/Cas9 Fucose Lens culinaris
Online-Zugang:	Volltext
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